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The map label for this gene is murE [H]

Identifier: 229917213

GI number: 229917213

Start: 1457526

End: 1458983

Strand: Reverse

Name: murE [H]

Synonym: EAT1b_1488

Alternate gene names: 229917213

Gene position: 1458983-1457526 (Counterclockwise)

Preceding gene: 229917214

Following gene: 229917212

Centisome position: 48.63

GC content: 49.86

Gene sequence:

>1458_bases
ATGAGAACGAAACGTTTATCCGCCATTTTACAAAATGAGATGGATGACATTTTTGATCCATGGGTGACCGGAGTCGAAAC
CGATTCCAGGAAGGTCAGTTCAGGGAATGTCTTTATTTGCATTCGCGGTTATACGGTTGACGGTCATAAGTTCGCGGAGG
ACGCCGTCGCCAACGGTGCGAGTGCGGTCATCTCAGAATATCCACTCGATCTCGATGTTCCGAATGTGGTTGTTGCCGAT
ACGAAACAGACGGCAGGAGAGGTTGCGAGTGTATTTTATGATCACCCCTCTCATCGGATGCGTATATACGGTGTGACAGG
AACGAATGGAAAAACTACGACGGCGACATTGACTTATGACTTGTTACGTCTTTCAGGACGTCGTGCAGGAATCGTGAGTA
CGATTGGCGCGCGTTTCCATAATCAAACGATTGAGACAGCCAATACGACACCAGAAGCCGTCGTCTTACATCGCATCTTA
GCAGAGATGGTCGATGCAGGCGTCACGGATTGCGTACTTGAAGTATCATCGCATGCCTTGATGGAAGGTCGCGTAGCGGG
AATCCACTTTCATGCGGCCGCGTTTACGAATTTGACGCATGACCATTTGGACTACCATCGTTCGATGGATGAGTATGCCC
GGGCCAAGGCGCGATTGTTTGAACAGGTGGAAGTGACGAACGGTGAGGCGATCGTGTTAAATGCGGAAGATTCGGCTAGT
CATGTCATGGCTGAGTTTGCGCCGACACGTCGAGCGATTATGTATAGCACGACAGTAGATGTAGAGGCGGATGTACAAGT
CATCTGGCGTGACGAGACGGTACTCGTCAGGATGAAGGAGTTCAAGCTCGAAGTGCCCGTTCGATTCATGGGGGCGTTCA
ATGCGGCGAACTTAGCAGCGGCAATCGGGCTCGTGTCGACTTCGGATATGGCGCTACGGACCATCGTTTCAAATGTTCCG
GGACTCACTTTGCCAAAAGGGCGGTTGGAACGTTTGGCTACAGAGGATGCTGAGATTTATATTGACTATGCCCACACACC
AGACGGGCTAGAGAAATGTCTATCCACATTGCAACAAGACGACCGTGCACTTGTCGTCGTGTTAAGTGCGGCAGGAGACC
GTGATCCTACGAAACGTCAAGATATGGGACGTATCGCAAGTCGCTATTCGAACGAGGTGATTGTCACCGTACATGATGTC
CGCATGGAAGACCCGAAACAAATCATTCGTGATTTAGTAAAAGGTATCCAACCGGATGTGAAGTATGTGACGTTTGAAGC
AAGGGAAGAGGCCCTTCGACATGCGGCCACGCAAGCTTTAGAAGGAAAGCGTGTCGTGGTCGTCGGAAAAGGACATGACC
ACATCGAACGGATCGGTCGCCAGTCGATTCCATTCAATGAATCGAACATTTTATTAGAAGAATTGAAAAAGCGAAAAGGG
CAGGAACCTGCCGTTTAA

Upstream 100 bases:

>100_bases
GAAGTAATATATTTGTCATCTCAATTGCCGTTAAAATCTAGTAACATGACGATAAGGTTTTATGAACCTAGCGACACCCA
TGAGAAAGGACGTACACAGC

Downstream 100 bases:

>100_bases
CAAAACGATGACGTTTGTCATCGTTTTTTAATGTGTGAAGAACAGGAAGGAAGGTAATAACTAATAATGTAGTAGAATTG
AAGGAGGAGAATAGACATGT

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MRTKRLSAILQNEMDDIFDPWVTGVETDSRKVSSGNVFICIRGYTVDGHKFAEDAVANGASAVISEYPLDLDVPNVVVAD
TKQTAGEVASVFYDHPSHRMRIYGVTGTNGKTTTATLTYDLLRLSGRRAGIVSTIGARFHNQTIETANTTPEAVVLHRIL
AEMVDAGVTDCVLEVSSHALMEGRVAGIHFHAAAFTNLTHDHLDYHRSMDEYARAKARLFEQVEVTNGEAIVLNAEDSAS
HVMAEFAPTRRAIMYSTTVDVEADVQVIWRDETVLVRMKEFKLEVPVRFMGAFNAANLAAAIGLVSTSDMALRTIVSNVP
GLTLPKGRLERLATEDAEIYIDYAHTPDGLEKCLSTLQQDDRALVVVLSAAGDRDPTKRQDMGRIASRYSNEVIVTVHDV
RMEDPKQIIRDLVKGIQPDVKYVTFEAREEALRHAATQALEGKRVVVVGKGHDHIERIGRQSIPFNESNILLEELKKRKG
QEPAV

Sequences:

>Translated_485_residues
MRTKRLSAILQNEMDDIFDPWVTGVETDSRKVSSGNVFICIRGYTVDGHKFAEDAVANGASAVISEYPLDLDVPNVVVAD
TKQTAGEVASVFYDHPSHRMRIYGVTGTNGKTTTATLTYDLLRLSGRRAGIVSTIGARFHNQTIETANTTPEAVVLHRIL
AEMVDAGVTDCVLEVSSHALMEGRVAGIHFHAAAFTNLTHDHLDYHRSMDEYARAKARLFEQVEVTNGEAIVLNAEDSAS
HVMAEFAPTRRAIMYSTTVDVEADVQVIWRDETVLVRMKEFKLEVPVRFMGAFNAANLAAAIGLVSTSDMALRTIVSNVP
GLTLPKGRLERLATEDAEIYIDYAHTPDGLEKCLSTLQQDDRALVVVLSAAGDRDPTKRQDMGRIASRYSNEVIVTVHDV
RMEDPKQIIRDLVKGIQPDVKYVTFEAREEALRHAATQALEGKRVVVVGKGHDHIERIGRQSIPFNESNILLEELKKRKG
QEPAV
>Mature_485_residues
MRTKRLSAILQNEMDDIFDPWVTGVETDSRKVSSGNVFICIRGYTVDGHKFAEDAVANGASAVISEYPLDLDVPNVVVAD
TKQTAGEVASVFYDHPSHRMRIYGVTGTNGKTTTATLTYDLLRLSGRRAGIVSTIGARFHNQTIETANTTPEAVVLHRIL
AEMVDAGVTDCVLEVSSHALMEGRVAGIHFHAAAFTNLTHDHLDYHRSMDEYARAKARLFEQVEVTNGEAIVLNAEDSAS
HVMAEFAPTRRAIMYSTTVDVEADVQVIWRDETVLVRMKEFKLEVPVRFMGAFNAANLAAAIGLVSTSDMALRTIVSNVP
GLTLPKGRLERLATEDAEIYIDYAHTPDGLEKCLSTLQQDDRALVVVLSAAGDRDPTKRQDMGRIASRYSNEVIVTVHDV
RMEDPKQIIRDLVKGIQPDVKYVTFEAREEALRHAATQALEGKRVVVVGKGHDHIERIGRQSIPFNESNILLEELKKRKG
QEPAV

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=479, Percent_Identity=32.5678496868476, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1786274, Length=125, Percent_Identity=34.4, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53574; Mature: 53574

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKRLSAILQNEMDDIFDPWVTGVETDSRKVSSGNVFICIRGYTVDGHKFAEDAVANGA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEEEEEECCHHHHHHHHCCCH
SAVISEYPLDLDVPNVVVADTKQTAGEVASVFYDHPSHRMRIYGVTGTNGKTTTATLTYD
HHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEHH
LLRLSGRRAGIVSTIGARFHNQTIETANTTPEAVVLHRILAEMVDAGVTDCVLEVSSHAL
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MEGRVAGIHFHAAAFTNLTHDHLDYHRSMDEYARAKARLFEQVEVTNGEAIVLNAEDSAS
HCCCEEEEEEEHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHEECCCCEEEEECCCCHH
HVMAEFAPTRRAIMYSTTVDVEADVQVIWRDETVLVRMKEFKLEVPVRFMGAFNAANLAA
HHHHHHCCCCEEEEEEEEECCCCCEEEEEECCEEEEEEECCEEECCCHHHCCCCHHHHHH
AIGLVSTSDMALRTIVSNVPGLTLPKGRLERLATEDAEIYIDYAHTPDGLEKCLSTLQQD
HHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCC
DRALVVVLSAAGDRDPTKRQDMGRIASRYSNEVIVTVHDVRMEDPKQIIRDLVKGIQPDV
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
KYVTFEAREEALRHAATQALEGKRVVVVGKGHDHIERIGRQSIPFNESNILLEELKKRKG
EEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHCC
QEPAV
CCCCC
>Mature Secondary Structure
MRTKRLSAILQNEMDDIFDPWVTGVETDSRKVSSGNVFICIRGYTVDGHKFAEDAVANGA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEEEEEECCHHHHHHHHCCCH
SAVISEYPLDLDVPNVVVADTKQTAGEVASVFYDHPSHRMRIYGVTGTNGKTTTATLTYD
HHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEHH
LLRLSGRRAGIVSTIGARFHNQTIETANTTPEAVVLHRILAEMVDAGVTDCVLEVSSHAL
HHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MEGRVAGIHFHAAAFTNLTHDHLDYHRSMDEYARAKARLFEQVEVTNGEAIVLNAEDSAS
HCCCEEEEEEEHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHEECCCCEEEEECCCCHH
HVMAEFAPTRRAIMYSTTVDVEADVQVIWRDETVLVRMKEFKLEVPVRFMGAFNAANLAA
HHHHHHCCCCEEEEEEEEECCCCCEEEEEECCEEEEEEECCEEECCCHHHCCCCHHHHHH
AIGLVSTSDMALRTIVSNVPGLTLPKGRLERLATEDAEIYIDYAHTPDGLEKCLSTLQQD
HHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCC
DRALVVVLSAAGDRDPTKRQDMGRIASRYSNEVIVTVHDVRMEDPKQIIRDLVKGIQPDV
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCC
KYVTFEAREEALRHAATQALEGKRVVVVGKGHDHIERIGRQSIPFNESNILLEELKKRKG
EEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHCC
QEPAV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA