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The map label for this gene is xylS [H]

Identifier: 229917198

GI number: 229917198

Start: 1440473

End: 1442815

Strand: Reverse

Name: xylS [H]

Synonym: EAT1b_1473

Alternate gene names: 229917198

Gene position: 1442815-1440473 (Counterclockwise)

Preceding gene: 229917199

Following gene: 229917197

Centisome position: 48.1

GC content: 51.73

Gene sequence:

>2343_bases
ATGCGCCTATTCGAAGAAAATCATGTGTACCAGTCGTGGATGAGACCAAAAGCAAGAGAAGAAGCAATCGTACAAGGAAG
CTGCTATCGCTTCACCGTATTGACGAGTCAGTTGATTCGAATGGAATACGCGGAAGACGGTGTCTTTGAAGACCGTGCCA
CCCAAGTAGTATGGAATCGGGAATTTGACGTTCCGCAATTCCGAGTGATTGAAGATGAGACAGAACTCCAAATCATCACA
GAACATGTCCATTTGTATTATACGAAAGGTCCGTTCGCACCGAACACACTCTACGTAGACGTGAAAGGGAACTTTAGCAC
GTATTATAGCCGCTACATGTTCAACGGTCCGGAGCGGACGCTGAAAGGGACGGCGCGGACACTCGACCATGTGGACGGAG
CGACGGAGTTAGAAGAAGGAATCCTCTCCAAACAAGGGTATGCGATCATTGATGATTCATCCTCGTTTTTGATGACAGAC
GATCGCTTCGTCGAGCCGAGACGAAAAGGGATTCATGACATTTACTACTTCGGATATGGCCATGACTATAAACAGGCACT
TCGAGACTTTTACACGTTGACGGGTCCGACGCCGATGCTTCCTCGTAAAGCGCTCGGGAACTGGTGGAGCCGCTACTGGC
GATACGACGAAAAAGAGTATAAGGCGTTGATGCGCCGTTTCAAGTCAGAAGATGTCCCGTTCTCGGTCAGTGTCATCGAT
ATGGATTGGCACGTGACGGACATCCCGGAAGAGTATGGGAGTGGTTGGACGGGCTATTCGTGGAACAAAAACTTGTTTCC
CGAACCGAAGCGTTTCCTTAACTGGTTAAAGGATGAGGGAATGATGGTGACGTTGAACCTGCATCCCGCGGACGGGGTCC
GTGCGTTTGAAGATCAGTACGTGGCGATGGCCGAGGCCATGGGGGGCGACCCGGAATCGGGGGACCGAATCCCATTCGAT
TTTTCAGACAAACGATTCATTCAAGCATATTTTGAGCAGATGCATCATCCACATGAGGCGGATGGCGTTGATTTTTGGTG
GATCGATTGGCAACAAGGGTCGACCTCGAAGATGGAAGGACTCGATCCGTTATGGATGTTAAATCATTATCATGCCATCG
ACATCGCCCGTGACGGTAAGCGTCCGCTCATTTTCTCTCGTTACGCAGGACCAGGGAGCCACCGTTACCCAGTCGGATTT
TCAGGAGACACGATCATCTCGTGGGCTTCGCTCGCCTTCCAGCCGTATTTTACAGCCACAGCGACAAATATCGGTTACGG
GTGGTGGAGTCATGACATCGGAGGCCATCATCGTGGGAAAAAAGACGATGAGCTCGCCGCGCGTTGGGTCGCATACGGGG
TATTCAGTCCAATCATGCGCCTCCACTCGACGTTCAGCACATTCAACGGGAAAGAGCCTTGGCGTTTCGGGATCGAAGCC
GAACAGTCGATGAAGCGATTCTTACGTCTGCGTCACGAGCTCATTCCTTATCTATACACGATGAATTATCGTAACCATGC
CAATGCGTTACCGCTCGTGTTGCCGATGTATATCGAACATCCCGATGACGAAGCGGCTTATCAAGTGCCGAATCAGTTTT
ATTTTGGCAGTGAACTCATTGTGTCCCCGATTGTCACGCCGATGGACCGAAAGCTCCACGTCGCCGGAACGAATGTCTGG
CTACCGAAAGGAGAATGGTTTGACTTCTTCACAGGACATCGTTACGAAGGCGACAAGTCGATTCGTGTGTTCCGTGGACT
CGATGGACAACCGGTCTTCGCCAAAGCGGGTGCCATCGTTCCCCTTATGCCGCATCAACCGCATGAGAACAAGACGGGGA
ATCCGAGCGAGCTAGAAGTACTCGTCTTTCCAGGTGCCTCGAACACGTTCACGATGTATGAAGATGACGGGGAAAGCCGC
GATTATGAGACAGGTGCCTCACTTGAAACGACGTTCACGCTCGACTGGGAAGCGCGGACGCTTCGCATCGAGATGTCAGG
AGACCTGACCCTCGTTCCGGCGAATCGGACATTGACCGTTCTTGTTCGAGGCGTTGTCGGGGAAGGGGACTATGACGCTG
AGACGCAGACGTTACACATCCCGATTGGTGAATTGAAGCAGGAGCAGACGGTGACGCTCCCCGTCGATGTGACACAGAAC
GCCCATCGACTTGACCGTCTCTATCGATTCTTAGATCGCGCGGAAATCGCCTACGATTTGAAAGATCGTTTGTACCAGAT
GGCGACGACGAAGACGCGTCTCGAGGCCTGGCTGTTTGACCTCCATGCGTTAGAACTCGATCGAGATTTGCAAGACGCCC
TATTAGAACTGATATTGGACTGA

Upstream 100 bases:

>100_bases
CGAAGGTTGTATGCCAAAAATGAGATTGCTATCATATTCAGTGTAAACGTTTGCACTAATTTCAGGAAGGAAGTGACGGA
GAGCGGAAACGCTCGAATGT

Downstream 100 bases:

>100_bases
AAAGGGGGGAGACCCCCTTTTGTCATGTCAGAAAACTGTCGGATTTTGAAAGCGGTATCATTCTGAATCGTTTTATACTT
AAGGTAGGGGATTTAACTAG

Product: glycoside hydrolase family 31

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 780; Mature: 780

Protein sequence:

>780_residues
MRLFEENHVYQSWMRPKAREEAIVQGSCYRFTVLTSQLIRMEYAEDGVFEDRATQVVWNREFDVPQFRVIEDETELQIIT
EHVHLYYTKGPFAPNTLYVDVKGNFSTYYSRYMFNGPERTLKGTARTLDHVDGATELEEGILSKQGYAIIDDSSSFLMTD
DRFVEPRRKGIHDIYYFGYGHDYKQALRDFYTLTGPTPMLPRKALGNWWSRYWRYDEKEYKALMRRFKSEDVPFSVSVID
MDWHVTDIPEEYGSGWTGYSWNKNLFPEPKRFLNWLKDEGMMVTLNLHPADGVRAFEDQYVAMAEAMGGDPESGDRIPFD
FSDKRFIQAYFEQMHHPHEADGVDFWWIDWQQGSTSKMEGLDPLWMLNHYHAIDIARDGKRPLIFSRYAGPGSHRYPVGF
SGDTIISWASLAFQPYFTATATNIGYGWWSHDIGGHHRGKKDDELAARWVAYGVFSPIMRLHSTFSTFNGKEPWRFGIEA
EQSMKRFLRLRHELIPYLYTMNYRNHANALPLVLPMYIEHPDDEAAYQVPNQFYFGSELIVSPIVTPMDRKLHVAGTNVW
LPKGEWFDFFTGHRYEGDKSIRVFRGLDGQPVFAKAGAIVPLMPHQPHENKTGNPSELEVLVFPGASNTFTMYEDDGESR
DYETGASLETTFTLDWEARTLRIEMSGDLTLVPANRTLTVLVRGVVGEGDYDAETQTLHIPIGELKQEQTVTLPVDVTQN
AHRLDRLYRFLDRAEIAYDLKDRLYQMATTKTRLEAWLFDLHALELDRDLQDALLELILD

Sequences:

>Translated_780_residues
MRLFEENHVYQSWMRPKAREEAIVQGSCYRFTVLTSQLIRMEYAEDGVFEDRATQVVWNREFDVPQFRVIEDETELQIIT
EHVHLYYTKGPFAPNTLYVDVKGNFSTYYSRYMFNGPERTLKGTARTLDHVDGATELEEGILSKQGYAIIDDSSSFLMTD
DRFVEPRRKGIHDIYYFGYGHDYKQALRDFYTLTGPTPMLPRKALGNWWSRYWRYDEKEYKALMRRFKSEDVPFSVSVID
MDWHVTDIPEEYGSGWTGYSWNKNLFPEPKRFLNWLKDEGMMVTLNLHPADGVRAFEDQYVAMAEAMGGDPESGDRIPFD
FSDKRFIQAYFEQMHHPHEADGVDFWWIDWQQGSTSKMEGLDPLWMLNHYHAIDIARDGKRPLIFSRYAGPGSHRYPVGF
SGDTIISWASLAFQPYFTATATNIGYGWWSHDIGGHHRGKKDDELAARWVAYGVFSPIMRLHSTFSTFNGKEPWRFGIEA
EQSMKRFLRLRHELIPYLYTMNYRNHANALPLVLPMYIEHPDDEAAYQVPNQFYFGSELIVSPIVTPMDRKLHVAGTNVW
LPKGEWFDFFTGHRYEGDKSIRVFRGLDGQPVFAKAGAIVPLMPHQPHENKTGNPSELEVLVFPGASNTFTMYEDDGESR
DYETGASLETTFTLDWEARTLRIEMSGDLTLVPANRTLTVLVRGVVGEGDYDAETQTLHIPIGELKQEQTVTLPVDVTQN
AHRLDRLYRFLDRAEIAYDLKDRLYQMATTKTRLEAWLFDLHALELDRDLQDALLELILD
>Mature_780_residues
MRLFEENHVYQSWMRPKAREEAIVQGSCYRFTVLTSQLIRMEYAEDGVFEDRATQVVWNREFDVPQFRVIEDETELQIIT
EHVHLYYTKGPFAPNTLYVDVKGNFSTYYSRYMFNGPERTLKGTARTLDHVDGATELEEGILSKQGYAIIDDSSSFLMTD
DRFVEPRRKGIHDIYYFGYGHDYKQALRDFYTLTGPTPMLPRKALGNWWSRYWRYDEKEYKALMRRFKSEDVPFSVSVID
MDWHVTDIPEEYGSGWTGYSWNKNLFPEPKRFLNWLKDEGMMVTLNLHPADGVRAFEDQYVAMAEAMGGDPESGDRIPFD
FSDKRFIQAYFEQMHHPHEADGVDFWWIDWQQGSTSKMEGLDPLWMLNHYHAIDIARDGKRPLIFSRYAGPGSHRYPVGF
SGDTIISWASLAFQPYFTATATNIGYGWWSHDIGGHHRGKKDDELAARWVAYGVFSPIMRLHSTFSTFNGKEPWRFGIEA
EQSMKRFLRLRHELIPYLYTMNYRNHANALPLVLPMYIEHPDDEAAYQVPNQFYFGSELIVSPIVTPMDRKLHVAGTNVW
LPKGEWFDFFTGHRYEGDKSIRVFRGLDGQPVFAKAGAIVPLMPHQPHENKTGNPSELEVLVFPGASNTFTMYEDDGESR
DYETGASLETTFTLDWEARTLRIEMSGDLTLVPANRTLTVLVRGVVGEGDYDAETQTLHIPIGELKQEQTVTLPVDVTQN
AHRLDRLYRFLDRAEIAYDLKDRLYQMATTKTRLEAWLFDLHALELDRDLQDALLELILD

Specific function: Catalyzes the liberation of alpha-xylose from the non- reducing terminal glucose of xyloglucan oligosaccharides. Has high hydrolytic activity on the disaccharide isoprimeverose. Follows a retaining mechanism of substrate hydrolysis [H]

COG id: COG1501

COG function: function code G; Alpha-glucosidases, family 31 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 31 family [H]

Homologues:

Organism=Homo sapiens, GI66346737, Length=513, Percent_Identity=25.5360623781676, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI38202257, Length=528, Percent_Identity=24.0530303030303, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI88900491, Length=528, Percent_Identity=24.0530303030303, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI119393895, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI119393893, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI119393891, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI157364974, Length=294, Percent_Identity=25.5102040816327, Blast_Score=94, Evalue=6e-19,
Organism=Homo sapiens, GI221316699, Length=194, Percent_Identity=25.7731958762887, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310115361, Length=224, Percent_Identity=24.5535714285714, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI2367256, Length=506, Percent_Identity=23.1225296442688, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI2367323, Length=249, Percent_Identity=27.3092369477912, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560800, Length=609, Percent_Identity=24.4663382594417, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560798, Length=609, Percent_Identity=24.4663382594417, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71991189, Length=500, Percent_Identity=23.2, Blast_Score=115, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71985706, Length=268, Percent_Identity=25.7462686567164, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32563849, Length=265, Percent_Identity=24.5283018867925, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319706, Length=365, Percent_Identity=24.6575342465753, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24643749, Length=572, Percent_Identity=22.2027972027972, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24643753, Length=572, Percent_Identity=22.2027972027972, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24643751, Length=572, Percent_Identity=22.2027972027972, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24643746, Length=572, Percent_Identity=22.2027972027972, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357605, Length=572, Percent_Identity=22.2027972027972, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011013
- InterPro:   IPR000322
- InterPro:   IPR017853 [H]

Pfam domain/function: PF01055 Glyco_hydro_31 [H]

EC number: NA

Molecular weight: Translated: 90626; Mature: 90626

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFEENHVYQSWMRPKAREEAIVQGSCYRFTVLTSQLIRMEYAEDGVFEDRATQVVWNR
CCCCCCCHHHHHHCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEECC
EFDVPQFRVIEDETELQIITEHVHLYYTKGPFAPNTLYVDVKGNFSTYYSRYMFNGPERT
CCCCCCEEEECCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCCHHHHHHHHEECCCCHH
LKGTARTLDHVDGATELEEGILSKQGYAIIDDSSSFLMTDDRFVEPRRKGIHDIYYFGYG
HHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHCCCCEEEEECCC
HDYKQALRDFYTLTGPTPMLPRKALGNWWSRYWRYDEKEYKALMRRFKSEDVPFSVSVID
CHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEE
MDWHVTDIPEEYGSGWTGYSWNKNLFPEPKRFLNWLKDEGMMVTLNLHPADGVRAFEDQY
ECCEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
VAMAEAMGGDPESGDRIPFDFSDKRFIQAYFEQMHHPHEADGVDFWWIDWQQGSTSKMEG
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHCC
LDPLWMLNHYHAIDIARDGKRPLIFSRYAGPGSHRYPVGFSGDTIISWASLAFQPYFTAT
CCCCHHHCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEE
ATNIGYGWWSHDIGGHHRGKKDDELAARWVAYGVFSPIMRLHSTFSTFNGKEPWRFGIEA
ECCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCH
EQSMKRFLRLRHELIPYLYTMNYRNHANALPLVLPMYIEHPDDEAAYQVPNQFYFGSELI
HHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEEEECCCCCHHHCCCCCEECCCHHH
VSPIVTPMDRKLHVAGTNVWLPKGEWFDFFTGHRYEGDKSIRVFRGLDGQPVFAKAGAIV
HCCCCCCCCCEEEEECCEEECCCCCCEECCCCCEECCCCCEEEEECCCCCCEEECCCEEE
PLMPHQPHENKTGNPSELEVLVFPGASNTFTMYEDDGESRDYETGASLETTFTLDWEART
ECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECEEE
LRIEMSGDLTLVPANRTLTVLVRGVVGEGDYDAETQTLHIPIGELKQEQTVTLPVDVTQN
EEEEECCCEEEEECCCEEEEEEEEHCCCCCCCCCCEEEEEEHHHCCCCCEEEEEEECCCC
AHRLDRLYRFLDRAEIAYDLKDRLYQMATTKTRLEAWLFDLHALELDRDLQDALLELILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MRLFEENHVYQSWMRPKAREEAIVQGSCYRFTVLTSQLIRMEYAEDGVFEDRATQVVWNR
CCCCCCCHHHHHHCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEECC
EFDVPQFRVIEDETELQIITEHVHLYYTKGPFAPNTLYVDVKGNFSTYYSRYMFNGPERT
CCCCCCEEEECCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCCHHHHHHHHEECCCCHH
LKGTARTLDHVDGATELEEGILSKQGYAIIDDSSSFLMTDDRFVEPRRKGIHDIYYFGYG
HHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHCCCCEEEEECCC
HDYKQALRDFYTLTGPTPMLPRKALGNWWSRYWRYDEKEYKALMRRFKSEDVPFSVSVID
CHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEE
MDWHVTDIPEEYGSGWTGYSWNKNLFPEPKRFLNWLKDEGMMVTLNLHPADGVRAFEDQY
ECCEEECCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
VAMAEAMGGDPESGDRIPFDFSDKRFIQAYFEQMHHPHEADGVDFWWIDWQQGSTSKMEG
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHCC
LDPLWMLNHYHAIDIARDGKRPLIFSRYAGPGSHRYPVGFSGDTIISWASLAFQPYFTAT
CCCCHHHCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEE
ATNIGYGWWSHDIGGHHRGKKDDELAARWVAYGVFSPIMRLHSTFSTFNGKEPWRFGIEA
ECCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCH
EQSMKRFLRLRHELIPYLYTMNYRNHANALPLVLPMYIEHPDDEAAYQVPNQFYFGSELI
HHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEEEECCCCCHHHCCCCCEECCCHHH
VSPIVTPMDRKLHVAGTNVWLPKGEWFDFFTGHRYEGDKSIRVFRGLDGQPVFAKAGAIV
HCCCCCCCCCEEEEECCEEECCCCCCEECCCCCEECCCCCEEEEECCCCCCEEECCCEEE
PLMPHQPHENKTGNPSELEVLVFPGASNTFTMYEDDGESRDYETGASLETTFTLDWEART
ECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECEEE
LRIEMSGDLTLVPANRTLTVLVRGVVGEGDYDAETQTLHIPIGELKQEQTVTLPVDVTQN
EEEEECCCEEEEECCCEEEEEEEEHCCCCCCCCCCEEEEEEHHHCCCCCEEEEEEECCCC
AHRLDRLYRFLDRAEIAYDLKDRLYQMATTKTRLEAWLFDLHALELDRDLQDALLELILD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10801892; 11427726 [H]