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The map label for this gene is gshAB [H]

Identifier: 229917148

GI number: 229917148

Start: 1391209

End: 1393500

Strand: Direct

Name: gshAB [H]

Synonym: EAT1b_1422

Alternate gene names: 229917148

Gene position: 1391209-1393500 (Clockwise)

Preceding gene: 229917147

Following gene: 229917149

Centisome position: 46.38

GC content: 44.68

Gene sequence:

>2292_bases
TTGCAGAAGCAGTTTAATCAACTACCCCACCATCTCATCACAAGTGGAAAATTTGCCATCGAGCGAGAAATGTTACGAGT
CACGTCAGAAGGCACACTAGCTGTTACGAAGCATCCAAAAGCACTCGGTCATAAAGCGACACATCCATACATCACGACCG
ACTTTTCTGAAAGTCAAATTGAGCTTGTCACCCCGACGTTTCCGACGATTGAGGCGGCACATCATTTCTTAGAAACGCTT
TACGATATTACGGCGCTCAACATAGGCGATGAACGCCTTTGGCCACAATCGATGCCATGTGCCTTGCCGGAAGGTGGTAC
GATTCCACTCGCTGATTTTGGTGTGTCCGGACTAGAGGCTAGCCGTTATCGTGAGCATCTGCTCCAAAAATATGGTGCGA
AAAAACAACTCATTTCTGGAATCCATTTCAACTTTTCATTTTCCGATGAGATGTTATCCGCATTATACGAAGGATCCCCT
GAACAATATCGACACTTCAAAGATCACCTATATTTGAAGTTAGTTCGTCAATATTTACGAATGCGTTGGCTCATCGTCTA
TTTGCTCGGTGCTACACCAGCCGTTCATGCGAGTTATGAAGAACGATGTGTCAGTCAGATGCAAGCGCTCCATGTAGACA
CTTTTTCAAATAAACAAGCATTGTCCTATCGGAATAGCGACTGTGGGTATACGAATGTTGAACCACTTTATCCAAATTAT
GCGACACTCTCTAGTTATATAGATGATGTAAGATGGTTCATTGCCAAAGGCGAGATTGCCAATCCGAACGAACTCTACTC
CCCCATCCGATTGAAAACATCGAAAGGTGGTAACGACTTAAATCAACTCGAGGCACACGGTATCGATTACATTGAAATTC
GCAATATCGATTTGAATCCGTTTGAAAAGACCGGAATTGCTCGTTCAGATTTACGGTTCCTTCATCTGTTTTTGTTGTAC
TTGACCTTTACTGAGGAGACATCTCATGTCGATTGGCAAGCTGAAGCGTACAATAATCAACAACGTGTCGCCAAAGAAGG
CATGTCTCCCGCAAGCATATTGACTCGAGATGGTTACGCCATACCTTTACACACGTACGCGACAGAACTTCTCGACGCCA
TCGATTCGTTCAACTCGCACTATGACCTCGGCTTCGAAGATGTCTTTCCCGCTATACGCGAAAAAATTGATCACGTGGAC
CGGACGTACGCTTCCCGATTAAAGACGATTATGATCGAGACAGGTTATCACGCATGGTCCCTCGCCCAAGCAGAACACTT
TTATGAGGACGCCTATGCAAATCGTTTTCGCATTCACGGTTATGCCGACCTGGAACTGTCTACTCAAATCTTAATGAAAT
CCGCAATTAAACGTGGTTATCGTGTCGATGTCATCGATCGTTCTGACCAGTTCATTCGGATTCGAAACGGCAAACACGAA
GAATACGTCAAACAGGCAACGAAAACATCAAAAGACAATTATGTTTCCGTCTTAATGATGGAGAATAAAACGGTGACAAA
GTTTGTACTCGCAGAGAATGGCATTCGTGTCCCTGAAGGGGTAGAACTTCAAGAGGAATCGGATTTATCTCAGGCATGGC
ATTCATTTTCAAAACAACCCATCGTCTTAAAGCCGAAGTCTACAAACTTCGGAGTCGGTATTCACATTTTTAAAGACGGA
GCGACGTATGAGGAAGCACTGTCTGCATTTCAGTATGCGAAAACGTTTGATGATGTCGTTTTACTTGAGACATTCTTACC
CGGAAAAGAATATCGCTTCCTCTTCATCGGGGATGATGTCGCGGGCATCCTCCATCGTGTCCCGGCGAACGTCACTGGAG
ATGGCACCTCTTCTATCCGTCAACTCGTTGCGACAAAAAATGAAGACCCGTTGCGTGGGAAAGGATACAAGACACCGCTC
GAGAAAATCGAAATCGACGAGGTCGTCATCTCATTTTTACGTGCTCAAGGCAAGACCCCCGATAGTATTCCCGCTCTCGA
TGAGCGTGTCTTCTTACGCGAAAACTCTAATATCAGTACCGGCGGAGATAGTATCGACTATACGGATCAAATTTCTGACA
TCTATAAGCGTATTGCTGTCGATGCCGCTAAGTCAGTCGGGGCAACCATCTGTGGTGTCGATATGATGATTCCCTCTCTC
GATGATGCGCAAATCGAATACGGTATCATCGAATTGAATTTTAATCCCGCCATCCATATCCACGCTTTTCCTTATGAAGG
AAAAGAACGACCGATTGGTGAGATGCTACTTGATTTATTGTTTAAATATTGA

Upstream 100 bases:

>100_bases
GGAATCGATGCTATCTGGTGGAGTATCGCCATCTCAAGTATCACAAAAGGGCTTGTCTCTGCCCTCTTTGTCCGACTAAC
GAAAAAGGAAGTGATTTCCC

Downstream 100 bases:

>100_bases
GTATTCCTGTTTCGGAATATTCCTAATTGGGAATATTCGAAATAAGAGGACGGTGACGAAAAATGGAAGAGAGATTGGCT
TCTTTGTTTCAAGCGTTAGC

Product: glutamate/cysteine ligase, /amino acid ligase

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MQKQFNQLPHHLITSGKFAIEREMLRVTSEGTLAVTKHPKALGHKATHPYITTDFSESQIELVTPTFPTIEAAHHFLETL
YDITALNIGDERLWPQSMPCALPEGGTIPLADFGVSGLEASRYREHLLQKYGAKKQLISGIHFNFSFSDEMLSALYEGSP
EQYRHFKDHLYLKLVRQYLRMRWLIVYLLGATPAVHASYEERCVSQMQALHVDTFSNKQALSYRNSDCGYTNVEPLYPNY
ATLSSYIDDVRWFIAKGEIANPNELYSPIRLKTSKGGNDLNQLEAHGIDYIEIRNIDLNPFEKTGIARSDLRFLHLFLLY
LTFTEETSHVDWQAEAYNNQQRVAKEGMSPASILTRDGYAIPLHTYATELLDAIDSFNSHYDLGFEDVFPAIREKIDHVD
RTYASRLKTIMIETGYHAWSLAQAEHFYEDAYANRFRIHGYADLELSTQILMKSAIKRGYRVDVIDRSDQFIRIRNGKHE
EYVKQATKTSKDNYVSVLMMENKTVTKFVLAENGIRVPEGVELQEESDLSQAWHSFSKQPIVLKPKSTNFGVGIHIFKDG
ATYEEALSAFQYAKTFDDVVLLETFLPGKEYRFLFIGDDVAGILHRVPANVTGDGTSSIRQLVATKNEDPLRGKGYKTPL
EKIEIDEVVISFLRAQGKTPDSIPALDERVFLRENSNISTGGDSIDYTDQISDIYKRIAVDAAKSVGATICGVDMMIPSL
DDAQIEYGIIELNFNPAIHIHAFPYEGKERPIGEMLLDLLFKY

Sequences:

>Translated_763_residues
MQKQFNQLPHHLITSGKFAIEREMLRVTSEGTLAVTKHPKALGHKATHPYITTDFSESQIELVTPTFPTIEAAHHFLETL
YDITALNIGDERLWPQSMPCALPEGGTIPLADFGVSGLEASRYREHLLQKYGAKKQLISGIHFNFSFSDEMLSALYEGSP
EQYRHFKDHLYLKLVRQYLRMRWLIVYLLGATPAVHASYEERCVSQMQALHVDTFSNKQALSYRNSDCGYTNVEPLYPNY
ATLSSYIDDVRWFIAKGEIANPNELYSPIRLKTSKGGNDLNQLEAHGIDYIEIRNIDLNPFEKTGIARSDLRFLHLFLLY
LTFTEETSHVDWQAEAYNNQQRVAKEGMSPASILTRDGYAIPLHTYATELLDAIDSFNSHYDLGFEDVFPAIREKIDHVD
RTYASRLKTIMIETGYHAWSLAQAEHFYEDAYANRFRIHGYADLELSTQILMKSAIKRGYRVDVIDRSDQFIRIRNGKHE
EYVKQATKTSKDNYVSVLMMENKTVTKFVLAENGIRVPEGVELQEESDLSQAWHSFSKQPIVLKPKSTNFGVGIHIFKDG
ATYEEALSAFQYAKTFDDVVLLETFLPGKEYRFLFIGDDVAGILHRVPANVTGDGTSSIRQLVATKNEDPLRGKGYKTPL
EKIEIDEVVISFLRAQGKTPDSIPALDERVFLRENSNISTGGDSIDYTDQISDIYKRIAVDAAKSVGATICGVDMMIPSL
DDAQIEYGIIELNFNPAIHIHAFPYEGKERPIGEMLLDLLFKY
>Mature_763_residues
MQKQFNQLPHHLITSGKFAIEREMLRVTSEGTLAVTKHPKALGHKATHPYITTDFSESQIELVTPTFPTIEAAHHFLETL
YDITALNIGDERLWPQSMPCALPEGGTIPLADFGVSGLEASRYREHLLQKYGAKKQLISGIHFNFSFSDEMLSALYEGSP
EQYRHFKDHLYLKLVRQYLRMRWLIVYLLGATPAVHASYEERCVSQMQALHVDTFSNKQALSYRNSDCGYTNVEPLYPNY
ATLSSYIDDVRWFIAKGEIANPNELYSPIRLKTSKGGNDLNQLEAHGIDYIEIRNIDLNPFEKTGIARSDLRFLHLFLLY
LTFTEETSHVDWQAEAYNNQQRVAKEGMSPASILTRDGYAIPLHTYATELLDAIDSFNSHYDLGFEDVFPAIREKIDHVD
RTYASRLKTIMIETGYHAWSLAQAEHFYEDAYANRFRIHGYADLELSTQILMKSAIKRGYRVDVIDRSDQFIRIRNGKHE
EYVKQATKTSKDNYVSVLMMENKTVTKFVLAENGIRVPEGVELQEESDLSQAWHSFSKQPIVLKPKSTNFGVGIHIFKDG
ATYEEALSAFQYAKTFDDVVLLETFLPGKEYRFLFIGDDVAGILHRVPANVTGDGTSSIRQLVATKNEDPLRGKGYKTPL
EKIEIDEVVISFLRAQGKTPDSIPALDERVFLRENSNISTGGDSIDYTDQISDIYKRIAVDAAKSVGATICGVDMMIPSL
DDAQIEYGIIELNFNPAIHIHAFPYEGKERPIGEMLLDLLFKY

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=459, Percent_Identity=33.9869281045752, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR007370
- InterPro:   IPR006335
- InterPro:   IPR020561 [H]

Pfam domain/function: PF01071 GARS_A; PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 86723; Mature: 86723

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQFNQLPHHLITSGKFAIEREMLRVTSEGTLAVTKHPKALGHKATHPYITTDFSESQI
CCCHHHHCCHHHHCCCCHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCEEEECCCCCEE
ELVTPTFPTIEAAHHFLETLYDITALNIGDERLWPQSMPCALPEGGTIPLADFGVSGLEA
EEECCCCCHHHHHHHHHHHHHHHHEECCCCHHCCCCCCCCCCCCCCCEEHHHCCCCCCCH
SRYREHLLQKYGAKKQLISGIHFNFSFSDEMLSALYEGSPEQYRHFKDHLYLKLVRQYLR
HHHHHHHHHHHCCHHHHHCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MRWLIVYLLGATPAVHASYEERCVSQMQALHVDTFSNKQALSYRNSDCGYTNVEPLYPNY
HHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCH
ATLSSYIDDVRWFIAKGEIANPNELYSPIRLKTSKGGNDLNQLEAHGIDYIEIRNIDLNP
HHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHCCCCEEEEECCCCCC
FEKTGIARSDLRFLHLFLLYLTFTEETSHVDWQAEAYNNQQRVAKEGMSPASILTRDGYA
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCHHHHHHCCCCCHHHEECCCCE
IPLHTYATELLDAIDSFNSHYDLGFEDVFPAIREKIDHVDRTYASRLKTIMIETGYHAWS
EEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHH
LAQAEHFYEDAYANRFRIHGYADLELSTQILMKSAIKRGYRVDVIDRSDQFIRIRNGKHE
HHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCHH
EYVKQATKTSKDNYVSVLMMENKTVTKFVLAENGIRVPEGVELQEESDLSQAWHSFSKQP
HHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IVLKPKSTNFGVGIHIFKDGATYEEALSAFQYAKTFDDVVLLETFLPGKEYRFLFIGDDV
EEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
AGILHRVPANVTGDGTSSIRQLVATKNEDPLRGKGYKTPLEKIEIDEVVISFLRAQGKTP
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DSIPALDERVFLRENSNISTGGDSIDYTDQISDIYKRIAVDAAKSVGATICGVDMMIPSL
CCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCC
DDAQIEYGIIELNFNPAIHIHAFPYEGKERPIGEMLLDLLFKY
CCCEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQKQFNQLPHHLITSGKFAIEREMLRVTSEGTLAVTKHPKALGHKATHPYITTDFSESQI
CCCHHHHCCHHHHCCCCHHHHHHHHHHCCCCCEEEECCCHHHCCCCCCCEEEECCCCCEE
ELVTPTFPTIEAAHHFLETLYDITALNIGDERLWPQSMPCALPEGGTIPLADFGVSGLEA
EEECCCCCHHHHHHHHHHHHHHHHEECCCCHHCCCCCCCCCCCCCCCEEHHHCCCCCCCH
SRYREHLLQKYGAKKQLISGIHFNFSFSDEMLSALYEGSPEQYRHFKDHLYLKLVRQYLR
HHHHHHHHHHHCCHHHHHCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MRWLIVYLLGATPAVHASYEERCVSQMQALHVDTFSNKQALSYRNSDCGYTNVEPLYPNY
HHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCH
ATLSSYIDDVRWFIAKGEIANPNELYSPIRLKTSKGGNDLNQLEAHGIDYIEIRNIDLNP
HHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHCCCCEEEEECCCCCC
FEKTGIARSDLRFLHLFLLYLTFTEETSHVDWQAEAYNNQQRVAKEGMSPASILTRDGYA
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCHHHHHHCCCCCHHHEECCCCE
IPLHTYATELLDAIDSFNSHYDLGFEDVFPAIREKIDHVDRTYASRLKTIMIETGYHAWS
EEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHH
LAQAEHFYEDAYANRFRIHGYADLELSTQILMKSAIKRGYRVDVIDRSDQFIRIRNGKHE
HHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCHH
EYVKQATKTSKDNYVSVLMMENKTVTKFVLAENGIRVPEGVELQEESDLSQAWHSFSKQP
HHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
IVLKPKSTNFGVGIHIFKDGATYEEALSAFQYAKTFDDVVLLETFLPGKEYRFLFIGDDV
EEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
AGILHRVPANVTGDGTSSIRQLVATKNEDPLRGKGYKTPLEKIEIDEVVISFLRAQGKTP
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DSIPALDERVFLRENSNISTGGDSIDYTDQISDIYKRIAVDAAKSVGATICGVDMMIPSL
CCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCC
DDAQIEYGIIELNFNPAIHIHAFPYEGKERPIGEMLLDLLFKY
CCCEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA