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The map label for this gene is lipA [H]

Identifier: 229917129

GI number: 229917129

Start: 1375200

End: 1376096

Strand: Direct

Name: lipA [H]

Synonym: EAT1b_1403

Alternate gene names: 229917129

Gene position: 1375200-1376096 (Clockwise)

Preceding gene: 229917127

Following gene: 229917130

Centisome position: 45.84

GC content: 51.17

Gene sequence:

>897_bases
GTGGCAAGAGGGGAACTGAAACGTAAGCCGGAATGGTTAAAGATTAAATTAAACACGAATGATACGTATACCGGCCTAAA
GAAAATGATGCGCGAGAAAAATTTACATACGGTATGTGAGGAAGCGAAATGTCCGAACATTCATGAATGTTGGGCGGTAC
GTCGGACCGCGACCTTCATGATTCTTGGAAGTATCTGTACACGTGCTTGCCGTTTTTGTGCCGTCACGACTGGGAAACCG
AACGAGCTCGATTGGGAAGAGCCAAAGCGTGTCGCCGAATCGGTTCGACTTATGAACTTGAAACACGTCGTCATCACGGC
TGTCGCGCGTGACGACTTGAACGATTACGGGGCTACTGTCTTCGCAGAAACGGTTCGCGCGGTTCGCGCCATGAACCCGG
AGACATCGATTGAGGTGCTCCCGTCAGACATGATGGGTGACTTCTCTGCCTTGCAGACGTTGATCGATGCGGGTCCTGAC
ATCATGAACCATAACTTAGAGACAGTCCGTCGCCTCACACCGAGAGTCCGTGCGAAAGCAACATATGACCGCACGCTCGA
GTTCTTGAAGCGTTCGAAAGAATTGAACCCGGATATCCCGACGAAGTCATCGATCATGGTTGGGCTTGGGGAGACGAAAG
AGGAATTGATTGAAGCGATGGATGACCTCCGCGCGCACAACGTCGACATCTTAACGCTCGGTCAATACTTGCAGCCGACG
AAGAAACACCTTGAGGTGGAACGTTACTACCATCCAGACGAGTTCGCCGAGTTGAAAGAGATCGCTCTATCAAAAGGATT
CTCACATTGTGAGGCTGGTCCACTCGTCCGTTCGTCATACCATGCGGATGAGCAAGTACACAAGGCGCGTCGCCACACGA
TGTTGCCAATCGACTAA

Upstream 100 bases:

>100_bases
CTGTATTGGCAAAACCAATAATTTCTGTGGTTCGTTTTTGATTCGTTTTTTGGTAAACTCAATTCGATACAGGTTAACTT
TCAAAAAGGGGGAAATTATT

Downstream 100 bases:

>100_bases
CAGAAAGAAGAGAGATTATGACTGGTACGACGTGGCATTTACTTAGAACTGAATCGACTTCCCCGGCCGAAAATATGGCG
ATTGATGAAGCCATCGCCAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MARGELKRKPEWLKIKLNTNDTYTGLKKMMREKNLHTVCEEAKCPNIHECWAVRRTATFMILGSICTRACRFCAVTTGKP
NELDWEEPKRVAESVRLMNLKHVVITAVARDDLNDYGATVFAETVRAVRAMNPETSIEVLPSDMMGDFSALQTLIDAGPD
IMNHNLETVRRLTPRVRAKATYDRTLEFLKRSKELNPDIPTKSSIMVGLGETKEELIEAMDDLRAHNVDILTLGQYLQPT
KKHLEVERYYHPDEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVHKARRHTMLPID

Sequences:

>Translated_298_residues
MARGELKRKPEWLKIKLNTNDTYTGLKKMMREKNLHTVCEEAKCPNIHECWAVRRTATFMILGSICTRACRFCAVTTGKP
NELDWEEPKRVAESVRLMNLKHVVITAVARDDLNDYGATVFAETVRAVRAMNPETSIEVLPSDMMGDFSALQTLIDAGPD
IMNHNLETVRRLTPRVRAKATYDRTLEFLKRSKELNPDIPTKSSIMVGLGETKEELIEAMDDLRAHNVDILTLGQYLQPT
KKHLEVERYYHPDEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVHKARRHTMLPID
>Mature_297_residues
ARGELKRKPEWLKIKLNTNDTYTGLKKMMREKNLHTVCEEAKCPNIHECWAVRRTATFMILGSICTRACRFCAVTTGKPN
ELDWEEPKRVAESVRLMNLKHVVITAVARDDLNDYGATVFAETVRAVRAMNPETSIEVLPSDMMGDFSALQTLIDAGPDI
MNHNLETVRRLTPRVRAKATYDRTLEFLKRSKELNPDIPTKSSIMVGLGETKEELIEAMDDLRAHNVDILTLGQYLQPTK
KHLEVERYYHPDEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVHKARRHTMLPID

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=300, Percent_Identity=44.3333333333333, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=45.2, Blast_Score=224, Evalue=6e-59,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=54.0350877192982, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=43.3566433566434, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=286, Percent_Identity=46.8531468531469, Blast_Score=265, Evalue=5e-72,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.9964664310954, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34033; Mature: 33901

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGELKRKPEWLKIKLNTNDTYTGLKKMMREKNLHTVCEEAKCPNIHECWAVRRTATFM
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ILGSICTRACRFCAVTTGKPNELDWEEPKRVAESVRLMNLKHVVITAVARDDLNDYGATV
HHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FAETVRAVRAMNPETSIEVLPSDMMGDFSALQTLIDAGPDIMNHNLETVRRLTPRVRAKA
HHHHHHHHHHCCCCCCEEECCHHHHCCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHH
TYDRTLEFLKRSKELNPDIPTKSSIMVGLGETKEELIEAMDDLRAHNVDILTLGQYLQPT
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCHH
KKHLEVERYYHPDEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVHKARRHTMLPID
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARGELKRKPEWLKIKLNTNDTYTGLKKMMREKNLHTVCEEAKCPNIHECWAVRRTATFM
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ILGSICTRACRFCAVTTGKPNELDWEEPKRVAESVRLMNLKHVVITAVARDDLNDYGATV
HHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FAETVRAVRAMNPETSIEVLPSDMMGDFSALQTLIDAGPDIMNHNLETVRRLTPRVRAKA
HHHHHHHHHHCCCCCCEEECCHHHHCCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHH
TYDRTLEFLKRSKELNPDIPTKSSIMVGLGETKEELIEAMDDLRAHNVDILTLGQYLQPT
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCHH
KKHLEVERYYHPDEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVHKARRHTMLPID
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA