Click here to switch to the map view.

The map label for this gene is yqiG [H]

Identifier: 229917079

GI number: 229917079

Start: 1322468

End: 1323583

Strand: Direct

Name: yqiG [H]

Synonym: EAT1b_1353

Alternate gene names: 229917079

Gene position: 1322468-1323583 (Clockwise)

Preceding gene: 229917069

Following gene: 229917086

Centisome position: 44.08

GC content: 54.12

Gene sequence:

>1116_bases
ATGAAACGTATGTTACAACCTTTTCAGTTCAAAAATGGCGTCGAAGTACCGAACCGCCTTGTATTGGCACCGATGACACA
TTACTCATCAGAGCCAACGGGCGAAGTATCCGAGCATGAGCTCGCCTACTATCGGAAACGGGCGCAAAACGTCGGGCTCG
CCATCACGGCTTGCGCGTACGTGACAGAAGACGGGAAAGGGTTCCCGAACGCATTCGGGGTCAATGACGACAAATTCATC
CCAGGTCTCCGTCAGTTGGCTGAAAGTTTAAAAGCGGAAGGCGCAAAAGCCATCCTTCAAATTTTCCACGCTGGGCGGAT
GTCACCACCTGAACTCGTCCCGAACGAACAACCTGTCAGTGCAAGTGCCGTCGCACCGGTCCGAGAAGGTGCCGTCACAC
CACGTGCCCTCGAGACGTCAGAAGTCGAAGCCATCATTGAGGCGTTCGGTGAAGCGACGCGTCGCGCCATCGAAGCCGGA
TTTGACGGAGTCGAAATTCACGGAGCGAACACCTACTTGATTCAACAATTCTTCTCACCGCATTCAAATCGCCGTGAAGA
TCGCTTCGGCGGTGATCTCCACGGACGTTTGACGTTCCCACGCGAAGTCATTCGTCGTGTCAAAGAAGCCGCTGCAGATG
CAGAAGACTTCCTCGTCGGCTATCGCTTCTCACCGGAAGAAATCGAAGAGCCAGGCATCACACTCGACGACACGGATGTG
TTACTCGATATGATCTTAGCAGAGGGTGTCGATTATATTCACGCGTCTCTCTGGAACTTCAACACAGCTTCCATGCGCGA
TGAGAACGTCACGGAGCGTTCGATTGACCGCATCGCCAAACGGATCAATGGTGCCATCCCACTCATCGGAGTCGGGCAAC
TCCAACGTCCGGCGCAAGTGGAAGACGCACTCGAGGTCGTGGACCTTGCCGCACTCGGTCGCGAACTGATCATCGACCCA
GAATGGCTCGAAAAAGTGAAATCGGACCGCGAAGACGAGATTGAGACCGAGCTCGACTTGAACAAGCAAGCCGAACTCGC
CGTCCCAGCTCCACTATGGAACGCCATCACGACACGTGCCGGTTGGTTCCCGATTAAAGATCAAGAAGTGAAATAA

Upstream 100 bases:

>100_bases
GCTTATGTAATCTGTAATGTGTTCATTCACAAGCATCCGACTCGCATTTTTGAGTCGATAAGCTTAGACTTCAAGGTATC
ATGTTGAAGGAGGATTTCAG

Downstream 100 bases:

>100_bases
AAAAATGAACCTGCCGTTTATCACGGCGGGTTCATTTACTATTTCAACGTCAAGAGCTCACTCATCGTCACAAACGTGTA
CCCTTCACGTTTCAGCGCAT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKRMLQPFQFKNGVEVPNRLVLAPMTHYSSEPTGEVSEHELAYYRKRAQNVGLAITACAYVTEDGKGFPNAFGVNDDKFI
PGLRQLAESLKAEGAKAILQIFHAGRMSPPELVPNEQPVSASAVAPVREGAVTPRALETSEVEAIIEAFGEATRRAIEAG
FDGVEIHGANTYLIQQFFSPHSNRREDRFGGDLHGRLTFPREVIRRVKEAAADAEDFLVGYRFSPEEIEEPGITLDDTDV
LLDMILAEGVDYIHASLWNFNTASMRDENVTERSIDRIAKRINGAIPLIGVGQLQRPAQVEDALEVVDLAALGRELIIDP
EWLEKVKSDREDEIETELDLNKQAELAVPAPLWNAITTRAGWFPIKDQEVK

Sequences:

>Translated_371_residues
MKRMLQPFQFKNGVEVPNRLVLAPMTHYSSEPTGEVSEHELAYYRKRAQNVGLAITACAYVTEDGKGFPNAFGVNDDKFI
PGLRQLAESLKAEGAKAILQIFHAGRMSPPELVPNEQPVSASAVAPVREGAVTPRALETSEVEAIIEAFGEATRRAIEAG
FDGVEIHGANTYLIQQFFSPHSNRREDRFGGDLHGRLTFPREVIRRVKEAAADAEDFLVGYRFSPEEIEEPGITLDDTDV
LLDMILAEGVDYIHASLWNFNTASMRDENVTERSIDRIAKRINGAIPLIGVGQLQRPAQVEDALEVVDLAALGRELIIDP
EWLEKVKSDREDEIETELDLNKQAELAVPAPLWNAITTRAGWFPIKDQEVK
>Mature_371_residues
MKRMLQPFQFKNGVEVPNRLVLAPMTHYSSEPTGEVSEHELAYYRKRAQNVGLAITACAYVTEDGKGFPNAFGVNDDKFI
PGLRQLAESLKAEGAKAILQIFHAGRMSPPELVPNEQPVSASAVAPVREGAVTPRALETSEVEAIIEAFGEATRRAIEAG
FDGVEIHGANTYLIQQFFSPHSNRREDRFGGDLHGRLTFPREVIRRVKEAAADAEDFLVGYRFSPEEIEEPGITLDDTDV
LLDMILAEGVDYIHASLWNFNTASMRDENVTERSIDRIAKRINGAIPLIGVGQLQRPAQVEDALEVVDLAALGRELIIDP
EWLEKVKSDREDEIETELDLNKQAELAVPAPLWNAITTRAGWFPIKDQEVK

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=333, Percent_Identity=30.6306306306306, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787939, Length=357, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17559804, Length=263, Percent_Identity=32.6996197718631, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=266, Percent_Identity=31.203007518797, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=179, Percent_Identity=35.7541899441341, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=256, Percent_Identity=30.078125, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17540738, Length=261, Percent_Identity=28.735632183908, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=178, Percent_Identity=32.5842696629214, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17566914, Length=264, Percent_Identity=25, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=261, Percent_Identity=28.735632183908, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321973, Length=235, Percent_Identity=28.936170212766, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 41227; Mature: 41227

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMLQPFQFKNGVEVPNRLVLAPMTHYSSEPTGEVSEHELAYYRKRAQNVGLAITACAY
CCCCCCCHHHCCCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEE
VTEDGKGFPNAFGVNDDKFIPGLRQLAESLKAEGAKAILQIFHAGRMSPPELVPNEQPVS
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCC
ASAVAPVREGAVTPRALETSEVEAIIEAFGEATRRAIEAGFDGVEIHGANTYLIQQFFSP
CHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
HSNRREDRFGGDLHGRLTFPREVIRRVKEAAADAEDFLVGYRFSPEEIEEPGITLDDTDV
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEECCCCHHHHCCCCCEECHHHH
LLDMILAEGVDYIHASLWNFNTASMRDENVTERSIDRIAKRINGAIPLIGVGQLQRPAQV
HHHHHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
EDALEVVDLAALGRELIIDPEWLEKVKSDREDEIETELDLNKQAELAVPAPLWNAITTRA
HHHHHHHHHHHHCHHHEECHHHHHHHHCCCHHHHHHHHCCCCCCCEECCCHHHHHHHHCC
GWFPIKDQEVK
CCCCCCCCCCC
>Mature Secondary Structure
MKRMLQPFQFKNGVEVPNRLVLAPMTHYSSEPTGEVSEHELAYYRKRAQNVGLAITACAY
CCCCCCCHHHCCCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEE
VTEDGKGFPNAFGVNDDKFIPGLRQLAESLKAEGAKAILQIFHAGRMSPPELVPNEQPVS
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCC
ASAVAPVREGAVTPRALETSEVEAIIEAFGEATRRAIEAGFDGVEIHGANTYLIQQFFSP
CHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCC
HSNRREDRFGGDLHGRLTFPREVIRRVKEAAADAEDFLVGYRFSPEEIEEPGITLDDTDV
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEECCCCHHHHCCCCCEECHHHH
LLDMILAEGVDYIHASLWNFNTASMRDENVTERSIDRIAKRINGAIPLIGVGQLQRPAQV
HHHHHHHHCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
EDALEVVDLAALGRELIIDPEWLEKVKSDREDEIETELDLNKQAELAVPAPLWNAITTRA
HHHHHHHHHHHHCHHHEECHHHHHHHHCCCHHHHHHHHCCCCCCCEECCCHHHHHHHHCC
GWFPIKDQEVK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]