Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 229917068

GI number: 229917068

Start: 1311294

End: 1312631

Strand: Reverse

Name: rho [H]

Synonym: EAT1b_1342

Alternate gene names: 229917068

Gene position: 1312631-1311294 (Counterclockwise)

Preceding gene: 229917070

Following gene: 229917067

Centisome position: 43.76

GC content: 49.63

Gene sequence:

>1338_bases
ATGAGTCAACAGACAGAACCGACAAAGAACTATACACTTCGTGAGCTTGAAACGAAGACGTTGAAAGAATTATATGAGAT
TGCCAAAGAAGTCAAAGTACCGAACTTCCGAGAAGCGCGGAAGCGTGAGCTCATGTTCCGTATTTTGAAATCACAGGCCG
AATCAAAAGATTTATACTTCCTCGAAGGTATTTTAGAGATCATCCCACCGAATCCACAGTCTCAAAATGATGGCGGATTT
GGATTCCTCCGACCAATCAACTATTCTCAATCTTCCGAAGACATCTATATCGCCGCATCGCAAATTCGTCGCTTCAATCT
TCGAAACGGAGACCTCGTCACAGGGAAGGTCCGTAAACCGAAAGAGAATGAACGGTTCCAAGGTTTGTTGAGTGTGGAAG
CGGTCAACGGAGAGTCGACGGACTCGACGCGTTCGCGTGATTATTTCCCGGCATTGACACCGATTTATCCAGAACAACAG
ATGGTGCTCGAGACAGAGCCGACCCATCTTTCGGTTCGTGTCATGGATATGATCGCACCAGTCGGGTTTGGCCAACGCGG
GTTGATTGTGGCACCACCAAAAGCGGGGAAAACGTCGCTTTTAAAAGAGATTGCCAACTCGATCACGACGAATCATCCGA
ACGTCGAATTGATCATTCTTCTAATCGATGAACGACCAGAGGAAGTGACGGATATTCAACGCTCTGTACCGGATGCGGAC
GTGGCGTTCTCGACTTTTGATGAGACACCGGAAAACCATGCGCGTATTTCAGAACTCGTTCTCGAACGCGCGATGCGTCT
CGTGGAACATAAAAAAGACGTGGTCATCCTACTCGATTCCATCACACGCTTGACGCGTGCGTACAACCTGTCTGTTCCAC
CAAGCGGACGGACACTCTCGGGTGGGATTGACCCGGCGGCGTTCCACAAACCGAAAAAGTTCTTCGGAGCGGCACGGAAT
ATCGAAGATGGGGGCAGCCTGACTATCCTCGCGACAGCGCTCGTCGATACCGGATCGCGAATGGACGACGTCATCTATGA
AGAGTTCAAAGGAACGGGGAACATGGAACTGCATCTGGACCGGAAATTGGCAGAGCGCCGGATCTTCCCTGCAATCGACA
TTCGTCGCTCGGGAACACGGAAAGAAGAGTTGTTACTCGGGAAGGACGAACTCGATTCGCTCTGGACGATTCGTCGTACG
ATGAACGAGTCTCCAGACTTCGTCGAACAGTTCTTACGCCGTGTCCGTGAGACGAAAACAAACCTCGACTTTTTTGCTGC
GCTAGAGGCAGATAAGAAAAAAAGACGACCGGTGAAAAAAACGAAAACAGACTTGTAA

Upstream 100 bases:

>100_bases
TACAGTGACGGCTTTGAAAAAATGTGGTTAGATGATGGCAGAACAAATGAATCAAGTTGAATTCTTCTTGTAATGAAATG
TAGAAAGATAGGTGACTTGA

Downstream 100 bases:

>100_bases
AACCTATAGTAGGGTGATATTATATTCTAGTGAACTTTCTCTAGGTTGAAAGAATACTTAGGGCCGAAGGAGTTGAAAAG
CAATGAAACAAGGAATCCAC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSQQTEPTKNYTLRELETKTLKELYEIAKEVKVPNFREARKRELMFRILKSQAESKDLYFLEGILEIIPPNPQSQNDGGF
GFLRPINYSQSSEDIYIAASQIRRFNLRNGDLVTGKVRKPKENERFQGLLSVEAVNGESTDSTRSRDYFPALTPIYPEQQ
MVLETEPTHLSVRVMDMIAPVGFGQRGLIVAPPKAGKTSLLKEIANSITTNHPNVELIILLIDERPEEVTDIQRSVPDAD
VAFSTFDETPENHARISELVLERAMRLVEHKKDVVILLDSITRLTRAYNLSVPPSGRTLSGGIDPAAFHKPKKFFGAARN
IEDGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRKLAERRIFPAIDIRRSGTRKEELLLGKDELDSLWTIRRT
MNESPDFVEQFLRRVRETKTNLDFFAALEADKKKRRPVKKTKTDL

Sequences:

>Translated_445_residues
MSQQTEPTKNYTLRELETKTLKELYEIAKEVKVPNFREARKRELMFRILKSQAESKDLYFLEGILEIIPPNPQSQNDGGF
GFLRPINYSQSSEDIYIAASQIRRFNLRNGDLVTGKVRKPKENERFQGLLSVEAVNGESTDSTRSRDYFPALTPIYPEQQ
MVLETEPTHLSVRVMDMIAPVGFGQRGLIVAPPKAGKTSLLKEIANSITTNHPNVELIILLIDERPEEVTDIQRSVPDAD
VAFSTFDETPENHARISELVLERAMRLVEHKKDVVILLDSITRLTRAYNLSVPPSGRTLSGGIDPAAFHKPKKFFGAARN
IEDGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRKLAERRIFPAIDIRRSGTRKEELLLGKDELDSLWTIRRT
MNESPDFVEQFLRRVRETKTNLDFFAALEADKKKRRPVKKTKTDL
>Mature_444_residues
SQQTEPTKNYTLRELETKTLKELYEIAKEVKVPNFREARKRELMFRILKSQAESKDLYFLEGILEIIPPNPQSQNDGGFG
FLRPINYSQSSEDIYIAASQIRRFNLRNGDLVTGKVRKPKENERFQGLLSVEAVNGESTDSTRSRDYFPALTPIYPEQQM
VLETEPTHLSVRVMDMIAPVGFGQRGLIVAPPKAGKTSLLKEIANSITTNHPNVELIILLIDERPEEVTDIQRSVPDADV
AFSTFDETPENHARISELVLERAMRLVEHKKDVVILLDSITRLTRAYNLSVPPSGRTLSGGIDPAAFHKPKKFFGAARNI
EDGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRKLAERRIFPAIDIRRSGTRKEELLLGKDELDSLWTIRRTM
NESPDFVEQFLRRVRETKTNLDFFAALEADKKKRRPVKKTKTDL

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=424, Percent_Identity=52.3584905660377, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 50632; Mature: 50500

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQTEPTKNYTLRELETKTLKELYEIAKEVKVPNFREARKRELMFRILKSQAESKDLYF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
LEGILEIIPPNPQSQNDGGFGFLRPINYSQSSEDIYIAASQIRRFNLRNGDLVTGKVRKP
HHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHCCCCCCCEEECCCCCC
KENERFQGLLSVEAVNGESTDSTRSRDYFPALTPIYPEQQMVLETEPTHLSVRVMDMIAP
CCCHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHC
VGFGQRGLIVAPPKAGKTSLLKEIANSITTNHPNVELIILLIDERPEEVTDIQRSVPDAD
CCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCH
VAFSTFDETPENHARISELVLERAMRLVEHKKDVVILLDSITRLTRAYNLSVPPSGRTLS
HEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHEECCCCCCCCCCC
GGIDPAAFHKPKKFFGAARNIEDGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLD
CCCCHHHHCCCHHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEEC
RKLAERRIFPAIDIRRSGTRKEELLLGKDELDSLWTIRRTMNESPDFVEQFLRRVRETKT
HHHHHHHCCCCEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
NLDFFAALEADKKKRRPVKKTKTDL
CCCEEEEECCCHHHCCCCCHHCCCC
>Mature Secondary Structure 
SQQTEPTKNYTLRELETKTLKELYEIAKEVKVPNFREARKRELMFRILKSQAESKDLYF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
LEGILEIIPPNPQSQNDGGFGFLRPINYSQSSEDIYIAASQIRRFNLRNGDLVTGKVRKP
HHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHCCCCCCCEEECCCCCC
KENERFQGLLSVEAVNGESTDSTRSRDYFPALTPIYPEQQMVLETEPTHLSVRVMDMIAP
CCCHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEHHHHHHC
VGFGQRGLIVAPPKAGKTSLLKEIANSITTNHPNVELIILLIDERPEEVTDIQRSVPDAD
CCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCH
VAFSTFDETPENHARISELVLERAMRLVEHKKDVVILLDSITRLTRAYNLSVPPSGRTLS
HEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHEECCCCCCCCCCC
GGIDPAAFHKPKKFFGAARNIEDGGSLTILATALVDTGSRMDDVIYEEFKGTGNMELHLD
CCCCHHHHCCCHHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEEC
RKLAERRIFPAIDIRRSGTRKEELLLGKDELDSLWTIRRTMNESPDFVEQFLRRVRETKT
HHHHHHHCCCCEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
NLDFFAALEADKKKRRPVKKTKTDL
CCCEEEEECCCHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]