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The map label for this gene is yxbG [H]

Identifier: 229917054

GI number: 229917054

Start: 1300908

End: 1301561

Strand: Direct

Name: yxbG [H]

Synonym: EAT1b_1328

Alternate gene names: 229917054

Gene position: 1300908-1301561 (Clockwise)

Preceding gene: 229917053

Following gene: 229917055

Centisome position: 43.37

GC content: 49.54

Gene sequence:

>654_bases
ATGTCAATGAAAACAGCAGTCATTATCGGAGCCGGTCCAGGAATTGGTCTTCATACAGCGGAACGATTCGCCAAAAATGG
TTATCAAGTCGCGTTACTCGCTCGAAACGCTGATCGTCTAATGGACTATGAAAAACAACTTGCAAAGAAAGGATATAAAG
CGAAACGGTTCGAAGTCGATACGCGATCAGAAGAGGATTTAAAATTGGTAATGGATCAAATCTATGACACGTTCGGCTCC
ATCGATGCGGTCGTCTACAACGCCATGGCTGCGACACCAGGTCGTCCAACTACGCTTTCTTCAGCGGACGTCATCCGCGA
TTTCGAAGTGAACGTACTTGGCGCATTGATGAGTGCACGCTTTGCCGCCCCGCTCATGAATACGGGTTCCATCTTGTTGA
CAGGTGGCGGACTCGCACTTTACCCGTCGGCAGACTACGCCTCGCTCGCCATCGGAAAAGCTGCACTCCGTAATCTCGCC
TATAGTCTTCATGAAGAATTGGCCGACTCCTCGATTTACGTCGGGACATTGACCATTAAAGGGTTCGTACAAGAGGGGAC
TTATTTCGCACCAGACCGCATCGCGGAAGCATTATATGACATGCATGTTCAGCAAACGGAAGTTGAGCGCATGTATGGGG
AGGACCATGCATGA

Upstream 100 bases:

>100_bases
TTCTCAGCAAGACGTTGCACGAAGCAAATGAACAACTCGAAAAAAAATAAAATTTTTTACACCCTCATGTGTATATACAA
CTATTAAGGAGGACATCATC

Downstream 100 bases:

>100_bases
GCACGCTCGTCATCCTCGCCCACCCGAACTTAGAAACGTCTCGTGTCAACAAAGCATGGAAAGAAGCTCTCGAACAGCAT
CATGAGGTGACTGTTCATGA

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSMKTAVIIGAGPGIGLHTAERFAKNGYQVALLARNADRLMDYEKQLAKKGYKAKRFEVDTRSEEDLKLVMDQIYDTFGS
IDAVVYNAMAATPGRPTTLSSADVIRDFEVNVLGALMSARFAAPLMNTGSILLTGGGLALYPSADYASLAIGKAALRNLA
YSLHEELADSSIYVGTLTIKGFVQEGTYFAPDRIAEALYDMHVQQTEVERMYGEDHA

Sequences:

>Translated_217_residues
MSMKTAVIIGAGPGIGLHTAERFAKNGYQVALLARNADRLMDYEKQLAKKGYKAKRFEVDTRSEEDLKLVMDQIYDTFGS
IDAVVYNAMAATPGRPTTLSSADVIRDFEVNVLGALMSARFAAPLMNTGSILLTGGGLALYPSADYASLAIGKAALRNLA
YSLHEELADSSIYVGTLTIKGFVQEGTYFAPDRIAEALYDMHVQQTEVERMYGEDHA
>Mature_216_residues
SMKTAVIIGAGPGIGLHTAERFAKNGYQVALLARNADRLMDYEKQLAKKGYKAKRFEVDTRSEEDLKLVMDQIYDTFGSI
DAVVYNAMAATPGRPTTLSSADVIRDFEVNVLGALMSARFAAPLMNTGSILLTGGGLALYPSADYASLAIGKAALRNLAY
SLHEELADSSIYVGTLTIKGFVQEGTYFAPDRIAEALYDMHVQQTEVERMYGEDHA

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI1787905, Length=133, Percent_Identity=33.0827067669173, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 23603; Mature: 23472

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKTAVIIGAGPGIGLHTAERFAKNGYQVALLARNADRLMDYEKQLAKKGYKAKRFEVD
CCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEECC
TRSEEDLKLVMDQIYDTFGSIDAVVYNAMAATPGRPTTLSSADVIRDFEVNVLGALMSAR
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FAAPLMNTGSILLTGGGLALYPSADYASLAIGKAALRNLAYSLHEELADSSIYVGTLTIK
HHHCCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
GFVQEGTYFAPDRIAEALYDMHVQQTEVERMYGEDHA
EECCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SMKTAVIIGAGPGIGLHTAERFAKNGYQVALLARNADRLMDYEKQLAKKGYKAKRFEVD
CCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEECC
TRSEEDLKLVMDQIYDTFGSIDAVVYNAMAATPGRPTTLSSADVIRDFEVNVLGALMSAR
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FAAPLMNTGSILLTGGGLALYPSADYASLAIGKAALRNLAYSLHEELADSSIYVGTLTIK
HHHCCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
GFVQEGTYFAPDRIAEALYDMHVQQTEVERMYGEDHA
EECCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584049; 9384377 [H]