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The map label for this gene is phoB [H]

Identifier: 229916985

GI number: 229916985

Start: 1232399

End: 1233073

Strand: Direct

Name: phoB [H]

Synonym: EAT1b_1259

Alternate gene names: 229916985

Gene position: 1232399-1233073 (Clockwise)

Preceding gene: 229916984

Following gene: 229916986

Centisome position: 41.08

GC content: 48.89

Gene sequence:

>675_bases
ATGAAGATTCTCATCGTAGACGATGAAGAAAAAATGAGACATTTGATTCGTTTGTTTCTTGAAAGTGACGGCTACTTTTG
CATCGAGGCGAGCGATGCCCGTTCCGCACTCGACAAAATCGAAGAGCGACCGGATCTCGTCCTTTTAGATATTATGATGC
CAGAAGTAGATGGGATCTCACTTTGTCGTCAGTTAAAGACGATGGATCCGGCATTACCTGTCGTCTTGTTGACGGCACGT
TCAGACAATGAGACGACCATCCAAGGATTAGATGCTGGAGCGGATGATTACGTCACCAAACCGTTCGATGGGAACGTGCT
CCTCGCTCGTATTCGTGCCGTCTTGCGCCGGACCCGTGTCGTCAACATCCAATACGGACCACTACGTTACGATGAGGAGA
GCGGACGCGTTTTGTATGAAGAACAAGAGATTGTCCTCACACCAAAAGCGACGGCCATCTTAAAATTGTTCCTTCAGCAC
CCGAATCGCCTCTTCAACCGCCTAGATTTGTTTGACCTTGTCTGGCCCTATTCTGCAGAATCCGACCCACGCACCGTTGA
TGCGCATATTCGTATGATTCGAGAAAAGTTAGGCGATGCAGGATTCCCGATTGATGATTATTTAAAGACGGTATGGGGAC
GTGGCTATCGATGGACCGAGGAGCGTTCCGGATGA

Upstream 100 bases:

>100_bases
GTCGAACGCGCACGTGAACAACTACAGACTCTTTTCGCCATGGAAGGAAATCACGCAAAGCACACGGAACGATAATAATA
AGAGGAAGGAGGAATGCAAG

Downstream 100 bases:

>100_bases
AGCGAGGTCGTAACAGTCTACCGTTTCGCATCAGCCTCGTCTTCCTGTTGTCTACGTTGTTGATTGAAGGAGTCTTATTC
TTACTCCTTTACCACACACT

Product: two component transcriptional regulator, winged helix family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKILIVDDEEKMRHLIRLFLESDGYFCIEASDARSALDKIEERPDLVLLDIMMPEVDGISLCRQLKTMDPALPVVLLTAR
SDNETTIQGLDAGADDYVTKPFDGNVLLARIRAVLRRTRVVNIQYGPLRYDEESGRVLYEEQEIVLTPKATAILKLFLQH
PNRLFNRLDLFDLVWPYSAESDPRTVDAHIRMIREKLGDAGFPIDDYLKTVWGRGYRWTEERSG

Sequences:

>Translated_224_residues
MKILIVDDEEKMRHLIRLFLESDGYFCIEASDARSALDKIEERPDLVLLDIMMPEVDGISLCRQLKTMDPALPVVLLTAR
SDNETTIQGLDAGADDYVTKPFDGNVLLARIRAVLRRTRVVNIQYGPLRYDEESGRVLYEEQEIVLTPKATAILKLFLQH
PNRLFNRLDLFDLVWPYSAESDPRTVDAHIRMIREKLGDAGFPIDDYLKTVWGRGYRWTEERSG
>Mature_224_residues
MKILIVDDEEKMRHLIRLFLESDGYFCIEASDARSALDKIEERPDLVLLDIMMPEVDGISLCRQLKTMDPALPVVLLTAR
SDNETTIQGLDAGADDYVTKPFDGNVLLARIRAVLRRTRVVNIQYGPLRYDEESGRVLYEEQEIVLTPKATAILKLFLQH
PNRLFNRLDLFDLVWPYSAESDPRTVDAHIRMIREKLGDAGFPIDDYLKTVWGRGYRWTEERSG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=39.5555555555556, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1790860, Length=201, Percent_Identity=34.8258706467662, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789809, Length=238, Percent_Identity=32.7731092436975, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=33.0357142857143, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=34.703196347032, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=33.9366515837104, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=33.0357142857143, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=34.0909090909091, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=29.5454545454545, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=36.4035087719298, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=28, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=29.5652173913043, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790437, Length=123, Percent_Identity=36.5853658536585, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788550, Length=103, Percent_Identity=40.7766990291262, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=31.4410480349345, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790299, Length=102, Percent_Identity=35.2941176470588, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=29.8701298701299, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=33.0578512396694, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI145693157, Length=120, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25799; Mature: 25799

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIVDDEEKMRHLIRLFLESDGYFCIEASDARSALDKIEERPDLVLLDIMMPEVDGIS
CEEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LCRQLKTMDPALPVVLLTARSDNETTIQGLDAGADDYVTKPFDGNVLLARIRAVLRRTRV
HHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
VNIQYGPLRYDEESGRVLYEEQEIVLTPKATAILKLFLQHPNRLFNRLDLFDLVWPYSAE
EEEEECCCEECCCCCCEEEECCEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC
SDPRTVDAHIRMIREKLGDAGFPIDDYLKTVWGRGYRWTEERSG
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKILIVDDEEKMRHLIRLFLESDGYFCIEASDARSALDKIEERPDLVLLDIMMPEVDGIS
CEEEEECCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCHHH
LCRQLKTMDPALPVVLLTARSDNETTIQGLDAGADDYVTKPFDGNVLLARIRAVLRRTRV
HHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
VNIQYGPLRYDEESGRVLYEEQEIVLTPKATAILKLFLQHPNRLFNRLDLFDLVWPYSAE
EEEEECCCEECCCCCCEEEECCEEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCC
SDPRTVDAHIRMIREKLGDAGFPIDDYLKTVWGRGYRWTEERSG
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]