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The map label for this gene is cwlS [H]

Identifier: 229916898

GI number: 229916898

Start: 1140355

End: 1142127

Strand: Direct

Name: cwlS [H]

Synonym: EAT1b_1171

Alternate gene names: 229916898

Gene position: 1140355-1142127 (Clockwise)

Preceding gene: 229916896

Following gene: 229916899

Centisome position: 38.01

GC content: 52.57

Gene sequence:

>1773_bases
ATGAATCCAAAGTCTGGTCTCACCTATTCATTCGCGGCACTTGCAGCGGTCAGCGTGCTTGCCCACGCAGACGAAGCAGC
AGCGGCGAGCACACATACGGTGCGTTCGGGTGATACGTTATGGTCGATCAGTCGGTACTATGATGTGTCCGTTGCGACGA
TTAAATCGTTGAATAATCTCTCGTCCGATAGCATTCGCGTCGGTCAAAAGCTGAAGCTGACAGGCAATTCATCTGCTACG
ACAGGTGGTTCCTCGTTGACCACGACGAGCACGAATCGCGTCACGACGACACACGCATTGAACATGCGGGTCGCCGGAGG
AACGTGGCATCGCGTCCTCTTGACGATTCCAAAAGGTACGACGCTCACACCAATCCAATCAACTGGATCGTGGACGAAAG
TCAGCTATGGCGGACAGACCGGTTGGGTGCATAACGATTATTTGCAAAAGGCATCTATGTCTAGCAGTACCGACAAACCA
TCAACGGTCACACCGAGCTCGGCTACAGCTCAAACGAAAGCCAACTTGAATCTGCGTTCGTCGAAGTCGACGAAGACGAC
CGTATTGCTCACGATTCCAAAAGGCAAGACCGTGACCGTCTTATCTGTTGAAGGGTCATGGTCGAAAGTGAAGTACGGGT
CGAAAACAGGATATGTCGCCAATACATATTTAACGACATCAGGAGCGGCGACACCGACAACACCGAGTACAGGACAATCG
ATCAATCAACAATTCACTACGACGGCAAACTTGAACGTTCGACAAGGTGCAGGCGTCGGTTATCCGCTCGTCACGACCAT
CCCGAACGGAACCGTCGTCAAAGCGACGAAACAGTCGGGCAGCTGGTACTATGTCACGTATAATGGGAAGTCCGGATACG
TGAGCGCCGGTTACTTGAAGCAGACGTCCACGACACCGAGCAACCCGGCTCCGAACGAAGGCGATGCCGGTGCTGGTAAT
GCGGCTGTCGATTATATCGTCAACACACCGTCCTTGAACGTACGTTCGAGCGCATCGACGAGTGCGACCATCATCGGGAG
TGTCAAAGCCGGACAGACGTTGCGCGTCGTCCAGTCATCGAAGGGATGGCTTCAGATTTATTACGGGAATACGGTCGGAT
TTGTCGCCTCGGCTTATGTGAAAACCGTCCCGAAAGGTTCGACAGACGGTCCGTCTTGGGTCGAAGGTTCTTACGATGCG
AACTCGTATTACACGTACTATCCGACATCGATTCGGTCACAGGCGAACGAGTCGAGCAGCACGGTCGGTTCGACACTGCG
TGGAGAACTATTGAACGTGATCGGAGAGACGGCGACCCACTATCGCCTCGCATCAGGATTCGTCGCAAAATCAGCCGTCA
CGGAAATCAAAGGGCAAAGCGCTCAGTCGACACGGCTTTCAACTATTGACGTGGCAAAACGCTACGTTGGAACACCGTAC
GTGTGGGCATCGTCTAGTCCGGCAAACGGTGGATTCGATTGTTCAGGACTCATCTATTATGTATTCAATCAATCCGGCGT
CAGCATCCCACGGACGAACGTGGCCAATTATTGGGGTGGCGCTTATTTCGGACCGCAATTGCCTAAGTCGTTCGTTCCCC
AGGCGGGTGACCTCGTCTTCTTCGAGAACACCTATATTGCCGGACCGTCGCACATGGGCATCATGATCAATTCTGATACG
TTCATCCATGCCGGTTCATCTGGATTAGGATACAACCAAATCAGTAAAGAACCGTATTGGCAGTCACGCTTAATCGGTTA
TAAGCGACCGTGA

Upstream 100 bases:

>100_bases
TTGCCCCATTGCTTGCCTATTTCTTTGCACCGTACAATGAACTTGTATTGTTTCACTTGTTGAATTGTTTCGTTCTCACT
CTTTGAAAGGATGTGACGCT

Downstream 100 bases:

>100_bases
CTTTGGTCACGCCAAAAAACCCGACGAAAGTCGGGTTTTTCTTTCGTTTTGCAGGATTTTCATCGCCTGACCCGAATTCG
GAAGTATGATGAAGGAAAAA

Product: NLP/P60 protein

Products: NA

Alternate protein names: Cell wall lytic enzyme associated with cell separation [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MNPKSGLTYSFAALAAVSVLAHADEAAAASTHTVRSGDTLWSISRYYDVSVATIKSLNNLSSDSIRVGQKLKLTGNSSAT
TGGSSLTTTSTNRVTTTHALNMRVAGGTWHRVLLTIPKGTTLTPIQSTGSWTKVSYGGQTGWVHNDYLQKASMSSSTDKP
STVTPSSATAQTKANLNLRSSKSTKTTVLLTIPKGKTVTVLSVEGSWSKVKYGSKTGYVANTYLTTSGAATPTTPSTGQS
INQQFTTTANLNVRQGAGVGYPLVTTIPNGTVVKATKQSGSWYYVTYNGKSGYVSAGYLKQTSTTPSNPAPNEGDAGAGN
AAVDYIVNTPSLNVRSSASTSATIIGSVKAGQTLRVVQSSKGWLQIYYGNTVGFVASAYVKTVPKGSTDGPSWVEGSYDA
NSYYTYYPTSIRSQANESSSTVGSTLRGELLNVIGETATHYRLASGFVAKSAVTEIKGQSAQSTRLSTIDVAKRYVGTPY
VWASSSPANGGFDCSGLIYYVFNQSGVSIPRTNVANYWGGAYFGPQLPKSFVPQAGDLVFFENTYIAGPSHMGIMINSDT
FIHAGSSGLGYNQISKEPYWQSRLIGYKRP

Sequences:

>Translated_590_residues
MNPKSGLTYSFAALAAVSVLAHADEAAAASTHTVRSGDTLWSISRYYDVSVATIKSLNNLSSDSIRVGQKLKLTGNSSAT
TGGSSLTTTSTNRVTTTHALNMRVAGGTWHRVLLTIPKGTTLTPIQSTGSWTKVSYGGQTGWVHNDYLQKASMSSSTDKP
STVTPSSATAQTKANLNLRSSKSTKTTVLLTIPKGKTVTVLSVEGSWSKVKYGSKTGYVANTYLTTSGAATPTTPSTGQS
INQQFTTTANLNVRQGAGVGYPLVTTIPNGTVVKATKQSGSWYYVTYNGKSGYVSAGYLKQTSTTPSNPAPNEGDAGAGN
AAVDYIVNTPSLNVRSSASTSATIIGSVKAGQTLRVVQSSKGWLQIYYGNTVGFVASAYVKTVPKGSTDGPSWVEGSYDA
NSYYTYYPTSIRSQANESSSTVGSTLRGELLNVIGETATHYRLASGFVAKSAVTEIKGQSAQSTRLSTIDVAKRYVGTPY
VWASSSPANGGFDCSGLIYYVFNQSGVSIPRTNVANYWGGAYFGPQLPKSFVPQAGDLVFFENTYIAGPSHMGIMINSDT
FIHAGSSGLGYNQISKEPYWQSRLIGYKRP
>Mature_590_residues
MNPKSGLTYSFAALAAVSVLAHADEAAAASTHTVRSGDTLWSISRYYDVSVATIKSLNNLSSDSIRVGQKLKLTGNSSAT
TGGSSLTTTSTNRVTTTHALNMRVAGGTWHRVLLTIPKGTTLTPIQSTGSWTKVSYGGQTGWVHNDYLQKASMSSSTDKP
STVTPSSATAQTKANLNLRSSKSTKTTVLLTIPKGKTVTVLSVEGSWSKVKYGSKTGYVANTYLTTSGAATPTTPSTGQS
INQQFTTTANLNVRQGAGVGYPLVTTIPNGTVVKATKQSGSWYYVTYNGKSGYVSAGYLKQTSTTPSNPAPNEGDAGAGN
AAVDYIVNTPSLNVRSSASTSATIIGSVKAGQTLRVVQSSKGWLQIYYGNTVGFVASAYVKTVPKGSTDGPSWVEGSYDA
NSYYTYYPTSIRSQANESSSTVGSTLRGELLNVIGETATHYRLASGFVAKSAVTEIKGQSAQSTRLSTIDVAKRYVGTPY
VWASSSPANGGFDCSGLIYYVFNQSGVSIPRTNVANYWGGAYFGPQLPKSFVPQAGDLVFFENTYIAGPSHMGIMINSDT
FIHAGSSGLGYNQISKEPYWQSRLIGYKRP

Specific function: Probably functions as a cell separation enzyme in addition to lytE and lytF [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell surface. Note=Localized at cell separation sites and cell poles during late vegetative phase [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=116, Percent_Identity=35.3448275862069, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: =3.4.19.11 [H]

Molecular weight: Translated: 62288; Mature: 62288

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKSGLTYSFAALAAVSVLAHADEAAAASTHTVRSGDTLWSISRYYDVSVATIKSLNNL
CCCCCCCEEHHHHHHHHHHHHHCCHHHCCCCEEECCCCCEEEEEEEEEEEHHHHHHHHCC
SSDSIRVGQKLKLTGNSSATTGGSSLTTTSTNRVTTTHALNMRVAGGTWHRVLLTIPKGT
CCCCEECCCEEEEECCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCEEEEEEECCCCC
TLTPIQSTGSWTKVSYGGQTGWVHNDYLQKASMSSSTDKPSTVTPSSATAQTKANLNLRS
EECEECCCCCEEEEEECCCCCCEEHHHHHHHCCCCCCCCCCEECCCCCCCEEEECEEEEC
SKSTKTTVLLTIPKGKTVTVLSVEGSWSKVKYGSKTGYVANTYLTTSGAATPTTPSTGQS
CCCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCCC
INQQFTTTANLNVRQGAGVGYPLVTTIPNGTVVKATKQSGSWYYVTYNGKSGYVSAGYLK
CCCEEEEEECCEEECCCCCCCCEEEECCCCEEEEEEECCCCEEEEEECCCCCEEEECEEE
QTSTTPSNPAPNEGDAGAGNAAVDYIVNTPSLNVRSSASTSATIIGSVKAGQTLRVVQSS
ECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCEEEEEECCCCCEEEEEECC
KGWLQIYYGNTVGFVASAYVKTVPKGSTDGPSWVEGSYDANSYYTYYPTSIRSQANESSS
CCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHH
TVGSTLRGELLNVIGETATHYRLASGFVAKSAVTEIKGQSAQSTRLSTIDVAKRYVGTPY
HHHHHHHHHHHHHHCCHHHHEEHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCE
VWASSSPANGGFDCSGLIYYVFNQSGVSIPRTNVANYWGGAYFGPQLPKSFVPQAGDLVF
EEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEE
FENTYIAGPSHMGIMINSDTFIHAGSSGLGYNQISKEPYWQSRLIGYKRP
EECEEECCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MNPKSGLTYSFAALAAVSVLAHADEAAAASTHTVRSGDTLWSISRYYDVSVATIKSLNNL
CCCCCCCEEHHHHHHHHHHHHHCCHHHCCCCEEECCCCCEEEEEEEEEEEHHHHHHHHCC
SSDSIRVGQKLKLTGNSSATTGGSSLTTTSTNRVTTTHALNMRVAGGTWHRVLLTIPKGT
CCCCEECCCEEEEECCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCEEEEEEECCCCC
TLTPIQSTGSWTKVSYGGQTGWVHNDYLQKASMSSSTDKPSTVTPSSATAQTKANLNLRS
EECEECCCCCEEEEEECCCCCCEEHHHHHHHCCCCCCCCCCEECCCCCCCEEEECEEEEC
SKSTKTTVLLTIPKGKTVTVLSVEGSWSKVKYGSKTGYVANTYLTTSGAATPTTPSTGQS
CCCCCEEEEEEECCCCEEEEEEECCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCCC
INQQFTTTANLNVRQGAGVGYPLVTTIPNGTVVKATKQSGSWYYVTYNGKSGYVSAGYLK
CCCEEEEEECCEEECCCCCCCCEEEECCCCEEEEEEECCCCEEEEEECCCCCEEEECEEE
QTSTTPSNPAPNEGDAGAGNAAVDYIVNTPSLNVRSSASTSATIIGSVKAGQTLRVVQSS
ECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCEEEEEECCCCCEEEEEECC
KGWLQIYYGNTVGFVASAYVKTVPKGSTDGPSWVEGSYDANSYYTYYPTSIRSQANESSS
CCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCCHH
TVGSTLRGELLNVIGETATHYRLASGFVAKSAVTEIKGQSAQSTRLSTIDVAKRYVGTPY
HHHHHHHHHHHHHHCCHHHHEEHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCE
VWASSSPANGGFDCSGLIYYVFNQSGVSIPRTNVANYWGGAYFGPQLPKSFVPQAGDLVF
EEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEE
FENTYIAGPSHMGIMINSDTFIHAGSSGLGYNQISKEPYWQSRLIGYKRP
EECEEECCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]