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The map label for this gene is yfkN [H]

Identifier: 229916796

GI number: 229916796

Start: 1024826

End: 1026904

Strand: Reverse

Name: yfkN [H]

Synonym: EAT1b_1069

Alternate gene names: 229916796

Gene position: 1026904-1024826 (Counterclockwise)

Preceding gene: 229916798

Following gene: 229916795

Centisome position: 34.23

GC content: 51.23

Gene sequence:

>2079_bases
ATGGCAAAGAAATCATCACGTAAACTGTATACAGCGCTTGCCGTAGCGGCTGTATCGACGGCATCACTCACGCCAGCTGC
TGTCACAGAAGCTGCACCGAAAGCATCGGTCGTCAAGGCGAAGTCAGGGTATGTATACAAAGGAGATTTGGATGCAGCAC
TCGACGCTACATATAAAGGGGCGCCAATCTACTGGTACAAATCAAGCGTTGACCTCGAGAAGCTCGGCACGTTCCAAACA
GCACGTGGGATCGTAAAAGGAAAAGGCATGTACATCGAGAAACGTGTACGCGTCCTCGATCATCCACTCGAAATCCTTCC
GCCGTCTGAGCCACTCGTGTTCAAACAAGGGAAGCCAGCAGTCGGAATCGTGAAGCAAGATGTGCGTTTCGCGAGCGGCA
CTTACGAAAAACCAGTTCGTTGGAGCGGGATCAACACAGATGAAGTCGGCGAATTCGTCGCGACGGCTACATACACGAAC
CGCGGAAAAACGATCACACTCGAAGTTCCTTATAAAGTAGAAGGATACAAGCTCTCGATCATGCACACGAACGACACGCA
CGCAGCGCTCAAGTACGCACCGAACCGTGCGACAGCAATCAAGCAAGTTCGTGCCGAGAAGCCAGATAGCCTCTTGATCG
ACGCAGGGGACGTCTTCTCAGGATCGCTCTACTTCAACGAGTTCAAAGGTCAAGCCGACCTCAAACTCATGAACTACATG
AAATACGACTTGATGGTGCCAGGTAACCACGAATTCGACCTCGGTACGGAGCAAGGACATAAAGAACTTTCTGACTTCGT
CCGCTATGCGAACTTCCCGTTCGTCAGCTCGAACGTGGACTATTCAAAAGACCAATACATGAAGAACTTGTACCGCAACG
AAACGACGGATAAGGCGTATAACGGTCGTTTGTATGAAGGGGTCATCAAAGTGGTCGACGGCAAGAAAGTCGGATTCTTC
GGTTTGACGACGGAAGAGACGGCTTCAATCGCTAGCCCAGGACCAATCGAATTCCAAAACTACATTGACGAAGCGCAAAA
AGCAGTCGATGCGTTCGAAGAGATGGGTGTCGACCAAATCGTCGCCGTGTCACACCTTGGTTTCAACGACAACCCAGCGT
TCGACAACGACCAGTTGCTTGCTGAGAACGTAGATGGCATCGACATCATCGTCGGTGGTCACACACATACGCGCTTGAAC
CAACCGGTCATGGTCACTGAAGGCAAAACTGAGCCGACATTGATCGTTCAAGCATATCAGTACAGCGAGTTCCTCGGGAC
ACTCGATGTCGAGTTCGACAAAGACGGGAAAATCGTCAAACATGCAGGTAGCTTGATTGATGTGAAGGCACTTCAGCCGG
ACCCATACGCGGTCTCACTTCTCGCACCGTTCAAAGAAGTGGTTGATGGCATCAGCAACGATCCAATCGGCGTCACGCTC
ACGGCACCACTTCTCAACCCACGTGTGGATGACGAAGGCAACGAAACCGGTGTGAGTGTTCGTAAAAACGAGACTGCACT
CGGAAACTTGATCACAGACGGCATGCTCACGAAAGCGAAAGAGTATGATTCAGCTGTCATCGGTGCCGTTCAAAACGGTG
GTGGAATCCGTGCGAAAATCGACCAAGGTCCAATCACGACAGGCGAAGTGTTGACGACACTTCCATTCGGAAACACGCTC
GCGATCATGGACTTGAAAGGTAGCGAATTGAAAGCGGCCTTCGAGCGTAGCGTTGGCGTTTACCCGCTCGAAAGTGGCGG
TTTCCTCCACGTATCTGGATTCAAAGTGTTGTTCGACAGCTCGAAACCGGCTGGCGAGCGCATCGTTAGCTTGGAATACA
ACAACGGCTCAGAATTCGTAGCAGTAGATGATGCGACGAGCTATAAAGTCGCAACGAACTTCTTCACAGCGCAAGGTGGA
GACAACTACGCTGAGTTCAAGAAGGCATTTGATGAAGGCCGCGTCAATGACCTCGGTCTCATCGACTGGGAAAACTTCCG
CGATCACTTGATCAGCCTCGGTGAAGAAGTCACACCGGCTGTTGAAGGACGGATCGTCGACGTTAATGCTGCAGAGTAA

Upstream 100 bases:

>100_bases
AAAACGGACGACTTTGGGGCAATGGGATTATACATAAGTCGAACGATGTAAGCGCTTTCCTTCGTGTGTCTTGTTCGCAA
ATCCGAAAGGGGAACTGGAC

Downstream 100 bases:

>100_bases
GTGAATAATGATTTTAGGGACGGATCCACGTGTGGGTCCGTCTTTTTGATGTGAAACGCCGGGCCGTACATTTGTCATCA
AATTGCGACCGACATGTAAC

Product: 5'-Nucleotidase domain protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MAKKSSRKLYTALAVAAVSTASLTPAAVTEAAPKASVVKAKSGYVYKGDLDAALDATYKGAPIYWYKSSVDLEKLGTFQT
ARGIVKGKGMYIEKRVRVLDHPLEILPPSEPLVFKQGKPAVGIVKQDVRFASGTYEKPVRWSGINTDEVGEFVATATYTN
RGKTITLEVPYKVEGYKLSIMHTNDTHAALKYAPNRATAIKQVRAEKPDSLLIDAGDVFSGSLYFNEFKGQADLKLMNYM
KYDLMVPGNHEFDLGTEQGHKELSDFVRYANFPFVSSNVDYSKDQYMKNLYRNETTDKAYNGRLYEGVIKVVDGKKVGFF
GLTTEETASIASPGPIEFQNYIDEAQKAVDAFEEMGVDQIVAVSHLGFNDNPAFDNDQLLAENVDGIDIIVGGHTHTRLN
QPVMVTEGKTEPTLIVQAYQYSEFLGTLDVEFDKDGKIVKHAGSLIDVKALQPDPYAVSLLAPFKEVVDGISNDPIGVTL
TAPLLNPRVDDEGNETGVSVRKNETALGNLITDGMLTKAKEYDSAVIGAVQNGGGIRAKIDQGPITTGEVLTTLPFGNTL
AIMDLKGSELKAAFERSVGVYPLESGGFLHVSGFKVLFDSSKPAGERIVSLEYNNGSEFVAVDDATSYKVATNFFTAQGG
DNYAEFKKAFDEGRVNDLGLIDWENFRDHLISLGEEVTPAVEGRIVDVNAAE

Sequences:

>Translated_692_residues
MAKKSSRKLYTALAVAAVSTASLTPAAVTEAAPKASVVKAKSGYVYKGDLDAALDATYKGAPIYWYKSSVDLEKLGTFQT
ARGIVKGKGMYIEKRVRVLDHPLEILPPSEPLVFKQGKPAVGIVKQDVRFASGTYEKPVRWSGINTDEVGEFVATATYTN
RGKTITLEVPYKVEGYKLSIMHTNDTHAALKYAPNRATAIKQVRAEKPDSLLIDAGDVFSGSLYFNEFKGQADLKLMNYM
KYDLMVPGNHEFDLGTEQGHKELSDFVRYANFPFVSSNVDYSKDQYMKNLYRNETTDKAYNGRLYEGVIKVVDGKKVGFF
GLTTEETASIASPGPIEFQNYIDEAQKAVDAFEEMGVDQIVAVSHLGFNDNPAFDNDQLLAENVDGIDIIVGGHTHTRLN
QPVMVTEGKTEPTLIVQAYQYSEFLGTLDVEFDKDGKIVKHAGSLIDVKALQPDPYAVSLLAPFKEVVDGISNDPIGVTL
TAPLLNPRVDDEGNETGVSVRKNETALGNLITDGMLTKAKEYDSAVIGAVQNGGGIRAKIDQGPITTGEVLTTLPFGNTL
AIMDLKGSELKAAFERSVGVYPLESGGFLHVSGFKVLFDSSKPAGERIVSLEYNNGSEFVAVDDATSYKVATNFFTAQGG
DNYAEFKKAFDEGRVNDLGLIDWENFRDHLISLGEEVTPAVEGRIVDVNAAE
>Mature_691_residues
AKKSSRKLYTALAVAAVSTASLTPAAVTEAAPKASVVKAKSGYVYKGDLDAALDATYKGAPIYWYKSSVDLEKLGTFQTA
RGIVKGKGMYIEKRVRVLDHPLEILPPSEPLVFKQGKPAVGIVKQDVRFASGTYEKPVRWSGINTDEVGEFVATATYTNR
GKTITLEVPYKVEGYKLSIMHTNDTHAALKYAPNRATAIKQVRAEKPDSLLIDAGDVFSGSLYFNEFKGQADLKLMNYMK
YDLMVPGNHEFDLGTEQGHKELSDFVRYANFPFVSSNVDYSKDQYMKNLYRNETTDKAYNGRLYEGVIKVVDGKKVGFFG
LTTEETASIASPGPIEFQNYIDEAQKAVDAFEEMGVDQIVAVSHLGFNDNPAFDNDQLLAENVDGIDIIVGGHTHTRLNQ
PVMVTEGKTEPTLIVQAYQYSEFLGTLDVEFDKDGKIVKHAGSLIDVKALQPDPYAVSLLAPFKEVVDGISNDPIGVTLT
APLLNPRVDDEGNETGVSVRKNETALGNLITDGMLTKAKEYDSAVIGAVQNGGGIRAKIDQGPITTGEVLTTLPFGNTLA
IMDLKGSELKAAFERSVGVYPLESGGFLHVSGFKVLFDSSKPAGERIVSLEYNNGSEFVAVDDATSYKVATNFFTAQGGD
NYAEFKKAFDEGRVNDLGLIDWENFRDHLISLGEEVTPAVEGRIVDVNAAE

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=554, Percent_Identity=31.7689530685921, Blast_Score=212, Evalue=1e-54,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=25.8652094717668, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922446, Length=544, Percent_Identity=29.7794117647059, Blast_Score=205, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24654424, Length=544, Percent_Identity=29.7794117647059, Blast_Score=205, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24641187, Length=571, Percent_Identity=27.1453590192644, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI19922444, Length=569, Percent_Identity=27.2407732864675, Blast_Score=175, Evalue=9e-44,
Organism=Drosophila melanogaster, GI28573524, Length=526, Percent_Identity=25.8555133079848, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI221329836, Length=565, Percent_Identity=25.1327433628319, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI19921980, Length=517, Percent_Identity=27.0793036750484, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24652512, Length=517, Percent_Identity=27.0793036750484, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161076508, Length=517, Percent_Identity=27.0793036750484, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 75401; Mature: 75269

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKSSRKLYTALAVAAVSTASLTPAAVTEAAPKASVVKAKSGYVYKGDLDAALDATYKG
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCEEEECCCCHHHCCCCCC
APIYWYKSSVDLEKLGTFQTARGIVKGKGMYIEKRVRVLDHPLEILPPSEPLVFKQGKPA
CEEEEEECCCCHHHHCCHHHHCCCCCCCCEEHHHHHHHHCCCHHHCCCCCCEEEECCCCC
VGIVKQDVRFASGTYEKPVRWSGINTDEVGEFVATATYTNRGKTITLEVPYKVEGYKLSI
EEEEECCCCCCCCCCCCCEEECCCCHHHHHHEEEEEEEECCCCEEEEEECEEECCEEEEE
MHTNDTHAALKYAPNRATAIKQVRAEKPDSLLIDAGDVFSGSLYFNEFKGQADLKLMNYM
EECCCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCCEECEEEEEECCCCCCCEEEEEE
KYDLMVPGNHEFDLGTEQGHKELSDFVRYANFPFVSSNVDYSKDQYMKNLYRNETTDKAY
EEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCHH
NGRLYEGVIKVVDGKKVGFFGLTTEETASIASPGPIEFQNYIDEAQKAVDAFEEMGVDQI
CCCCEEEEEEEECCCEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
VAVSHLGFNDNPAFDNDQLLAENVDGIDIIVGGHTHTRLNQPVMVTEGKTEPTLIVQAYQ
EEEEECCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEEEEEE
YSEFLGTLDVEFDKDGKIVKHAGSLIDVKALQPDPYAVSLLAPFKEVVDGISNDPIGVTL
HHHHHEEEEEEECCCCCEEEECCCEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCEEEEE
TAPLLNPRVDDEGNETGVSVRKNETALGNLITDGMLTKAKEYDSAVIGAVQNGGGIRAKI
EECCCCCCCCCCCCCCCCEEECCCCHHHHHHHCCHHHHHHHHCCEEEEEEECCCCEEEEE
DQGPITTGEVLTTLPFGNTLAIMDLKGSELKAAFERSVGVYPLESGGFLHVSGFKVLFDS
CCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCEEEEECEEEEEEC
SKPAGERIVSLEYNNGSEFVAVDDATSYKVATNFFTAQGGDNYAEFKKAFDEGRVNDLGL
CCCCCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEE
IDWENFRDHLISLGEEVTPAVEGRIVDVNAAE
EEHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
AKKSSRKLYTALAVAAVSTASLTPAAVTEAAPKASVVKAKSGYVYKGDLDAALDATYKG
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEECCCEEEECCCCHHHCCCCCC
APIYWYKSSVDLEKLGTFQTARGIVKGKGMYIEKRVRVLDHPLEILPPSEPLVFKQGKPA
CEEEEEECCCCHHHHCCHHHHCCCCCCCCEEHHHHHHHHCCCHHHCCCCCCEEEECCCCC
VGIVKQDVRFASGTYEKPVRWSGINTDEVGEFVATATYTNRGKTITLEVPYKVEGYKLSI
EEEEECCCCCCCCCCCCCEEECCCCHHHHHHEEEEEEEECCCCEEEEEECEEECCEEEEE
MHTNDTHAALKYAPNRATAIKQVRAEKPDSLLIDAGDVFSGSLYFNEFKGQADLKLMNYM
EECCCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCCEECEEEEEECCCCCCCEEEEEE
KYDLMVPGNHEFDLGTEQGHKELSDFVRYANFPFVSSNVDYSKDQYMKNLYRNETTDKAY
EEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCHH
NGRLYEGVIKVVDGKKVGFFGLTTEETASIASPGPIEFQNYIDEAQKAVDAFEEMGVDQI
CCCCEEEEEEEECCCEEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
VAVSHLGFNDNPAFDNDQLLAENVDGIDIIVGGHTHTRLNQPVMVTEGKTEPTLIVQAYQ
EEEEECCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCEEEECCCCCCEEEEEEEE
YSEFLGTLDVEFDKDGKIVKHAGSLIDVKALQPDPYAVSLLAPFKEVVDGISNDPIGVTL
HHHHHEEEEEEECCCCCEEEECCCEEEEEEECCCCCEEEHHHHHHHHHCCCCCCCEEEEE
TAPLLNPRVDDEGNETGVSVRKNETALGNLITDGMLTKAKEYDSAVIGAVQNGGGIRAKI
EECCCCCCCCCCCCCCCCEEECCCCHHHHHHHCCHHHHHHHHCCEEEEEEECCCCEEEEE
DQGPITTGEVLTTLPFGNTLAIMDLKGSELKAAFERSVGVYPLESGGFLHVSGFKVLFDS
CCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCEEEEECEEEEEEC
SKPAGERIVSLEYNNGSEFVAVDDATSYKVATNFFTAQGGDNYAEFKKAFDEGRVNDLGL
CCCCCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEE
IDWENFRDHLISLGEEVTPAVEGRIVDVNAAE
EEHHHHHHHHHHCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]