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The map label for this gene is dus2 [H]

Identifier: 229916707

GI number: 229916707

Start: 921946

End: 922938

Strand: Reverse

Name: dus2 [H]

Synonym: EAT1b_0980

Alternate gene names: 229916707

Gene position: 922938-921946 (Counterclockwise)

Preceding gene: 229916708

Following gene: 229916706

Centisome position: 30.77

GC content: 52.47

Gene sequence:

>993_bases
ATGAAAGAAAACTTTTGGCGCGATCTTCCGCGTCCATTCTTTATACTTGCCCCGATGGAAGATGTGACTGATGTCGTTTT
CCGCCACGTCGTCGCCCATGCGGGACGCCCGGACGTCTTTTTCACAGAATTCGCAAATTCGGATAGCTATTGCCATCCGG
AAGGGAAAGGCAGCCTCCGTGGCCGTTTGACGTTCACCGAGGACGAGCAGCCGATGGTCGCCCACATCTGGGGAGACAAT
CCGGAATACTTCCGTCAAATGAGTATCGGGATGGCCGAGATGGGCTATAAAGGACTCGATATCAATATGGGTTGCCCGGT
ACCGAACGTCGCGAAACGTGGAAAGGGGAGCGGACTCATTCTCCGTCCTGAAGTTGCGGCTGAGCTCATTCAAGCAGCAA
AAGCCGGCGGCATCCCGGTCAGCGTCAAAACGCGTCTCGGTTTCGCGAAGCTCGATGAGTGGCGTGACTGGTTGACGCAT
CTCTTGAAACAAGACATCGCGAACTTGTCCATTCACTTGCGCACGCGAAACGAGATGAGTAAAGTCGACGCGCACTGGGA
GATGATCCCGGACATCGTCAAATTGCGTGACGAGATTGCACCGGATACGCTCATCACGATCAACGGGGACATCCTCGACC
GTCAAATGGGACTCGAACTCGTTGAGAAATACGGGGTCGATGGAGTCATGATCGGTCGTGGGATCTTCAAGAACCCGTTC
GCGTTTGAGAAAGAGCCACGTGAGCACTCGATGGAAGAACATCTTGATCTATTGCGTCTGCAACTGGACCTTCAGGATAA
ATTCCAAGAAGACGTGCCGCGTTCGATTTCGAATTTGCACCGTTTCTTCAAGATTTATGTCAAAGGATTCCGTGGGGCGA
GCGAGCTACGCCATAACTTGATGGAAACGAAATCGACGGATGAGGTACGAGCGTTACTCGATGCCTTTGTCGAGAAATAT
CATGAAGAAGAGGCAGCGCTGGCTGCTGAATAA

Upstream 100 bases:

>100_bases
AACGGATTATGAGTAATGTCAGTTGATACAATCCGACGGACATAATAGAATAGATGAGTGATTTTGTTGGACTGTATGGA
TGAAAGGAACTGATTTTACG

Downstream 100 bases:

>100_bases
GCTTACTGGATACGAGGCGTACTCGTATCTTTTTTTGCAGGAAAAGTCATTTACGGGACGAATGAGAACAAGAGAGATAA
AAAGGAGGGAGAGCGGATGC

Product: dihydrouridine synthase DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKENFWRDLPRPFFILAPMEDVTDVVFRHVVAHAGRPDVFFTEFANSDSYCHPEGKGSLRGRLTFTEDEQPMVAHIWGDN
PEYFRQMSIGMAEMGYKGLDINMGCPVPNVAKRGKGSGLILRPEVAAELIQAAKAGGIPVSVKTRLGFAKLDEWRDWLTH
LLKQDIANLSIHLRTRNEMSKVDAHWEMIPDIVKLRDEIAPDTLITINGDILDRQMGLELVEKYGVDGVMIGRGIFKNPF
AFEKEPREHSMEEHLDLLRLQLDLQDKFQEDVPRSISNLHRFFKIYVKGFRGASELRHNLMETKSTDEVRALLDAFVEKY
HEEEAALAAE

Sequences:

>Translated_330_residues
MKENFWRDLPRPFFILAPMEDVTDVVFRHVVAHAGRPDVFFTEFANSDSYCHPEGKGSLRGRLTFTEDEQPMVAHIWGDN
PEYFRQMSIGMAEMGYKGLDINMGCPVPNVAKRGKGSGLILRPEVAAELIQAAKAGGIPVSVKTRLGFAKLDEWRDWLTH
LLKQDIANLSIHLRTRNEMSKVDAHWEMIPDIVKLRDEIAPDTLITINGDILDRQMGLELVEKYGVDGVMIGRGIFKNPF
AFEKEPREHSMEEHLDLLRLQLDLQDKFQEDVPRSISNLHRFFKIYVKGFRGASELRHNLMETKSTDEVRALLDAFVEKY
HEEEAALAAE
>Mature_330_residues
MKENFWRDLPRPFFILAPMEDVTDVVFRHVVAHAGRPDVFFTEFANSDSYCHPEGKGSLRGRLTFTEDEQPMVAHIWGDN
PEYFRQMSIGMAEMGYKGLDINMGCPVPNVAKRGKGSGLILRPEVAAELIQAAKAGGIPVSVKTRLGFAKLDEWRDWLTH
LLKQDIANLSIHLRTRNEMSKVDAHWEMIPDIVKLRDEIAPDTLITINGDILDRQMGLELVEKYGVDGVMIGRGIFKNPF
AFEKEPREHSMEEHLDLLRLQLDLQDKFQEDVPRSISNLHRFFKIYVKGFRGASELRHNLMETKSTDEVRALLDAFVEKY
HEEEAALAAE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=327, Percent_Identity=24.7706422018349, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789660, Length=325, Percent_Identity=27.6923076923077, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788462, Length=325, Percent_Identity=26.1538461538462, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI145693211, Length=251, Percent_Identity=26.2948207171315, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=216, Percent_Identity=31.0185185185185, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=260, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=312, Percent_Identity=26.2820512820513, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921524, Length=238, Percent_Identity=26.890756302521, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45549423, Length=182, Percent_Identity=30.2197802197802, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37748; Mature: 37748

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENFWRDLPRPFFILAPMEDVTDVVFRHVVAHAGRPDVFFTEFANSDSYCHPEGKGSLR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCE
GRLTFTEDEQPMVAHIWGDNPEYFRQMSIGMAEMGYKGLDINMGCPVPNVAKRGKGSGLI
EEEEECCCCCCEEEEEECCCHHHHHHHHCCHHHCCCCCCEEECCCCCCHHHHCCCCCCEE
LRPEVAAELIQAAKAGGIPVSVKTRLGFAKLDEWRDWLTHLLKQDIANLSIHLRTRNEMS
ECHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHH
KVDAHWEMIPDIVKLRDEIAPDTLITINGDILDRQMGLELVEKYGVDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSMEEHLDLLRLQLDLQDKFQEDVPRSISNLHRFFKIYVKGFRGASELRHNL
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
METKSTDEVRALLDAFVEKYHEEEAALAAE
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKENFWRDLPRPFFILAPMEDVTDVVFRHVVAHAGRPDVFFTEFANSDSYCHPEGKGSLR
CCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCE
GRLTFTEDEQPMVAHIWGDNPEYFRQMSIGMAEMGYKGLDINMGCPVPNVAKRGKGSGLI
EEEEECCCCCCEEEEEECCCHHHHHHHHCCHHHCCCCCCEEECCCCCCHHHHCCCCCCEE
LRPEVAAELIQAAKAGGIPVSVKTRLGFAKLDEWRDWLTHLLKQDIANLSIHLRTRNEMS
ECHHHHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHH
KVDAHWEMIPDIVKLRDEIAPDTLITINGDILDRQMGLELVEKYGVDGVMIGRGIFKNPF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
AFEKEPREHSMEEHLDLLRLQLDLQDKFQEDVPRSISNLHRFFKIYVKGFRGASELRHNL
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
METKSTDEVRALLDAFVEKYHEEEAALAAE
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]