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The map label for this gene is crr [H]

Identifier: 229916685

GI number: 229916685

Start: 904629

End: 905129

Strand: Reverse

Name: crr [H]

Synonym: EAT1b_0958

Alternate gene names: 229916685

Gene position: 905129-904629 (Counterclockwise)

Preceding gene: 229916687

Following gene: 229916684

Centisome position: 30.17

GC content: 49.5

Gene sequence:

>501_bases
ATGGGATTCTTTAAAAAACTTTTTGGTGGTAAAGAAGAACAACTCAACGCGGTTTCACCGCTTACAGGAAAGGTCGTCGA
CATCACGAACGTACCGGACCAAGTATTCTCACAAAAAATGATGGGTGACGGGATTGCGATCGAGCCGACAGAAGGAAAAG
TCGTCTCACCGGTTAGCGGAACAATCGCGACAGTCTTCCCAACAAAACACGCGATCGGTATCAACGCGGACAACGGTGCG
GAATACTTGATTCACATCGGTTTGGACACGGTCAACCTTAAAGGTGAAGGCTTCGAGACACACGTTACAGAAGGTCAGTC
TGTCAAAGCAGGCGACCCACTCGTCACGTTCGACCTCGACTTCATCAAAGCGAACGCGCCATCGACAATCACTCCGATCA
TCATCACGAACCACGACAGCTTCGGCGTGAACAAACTTGTCGCAGAAGGCGACACGGTGACAGCTGGATCTTCGGAAGTC
GTCGAATTGACAAAGAAATAA

Upstream 100 bases:

>100_bases
TTACAACTATACCGTTAATGGGGTAGGGATTGAAGTATTAGCTTTTCGGGTATAAAAGTGGAGACTTCGATTCAATAGAA
CATGTTGGAGGGATTAAGCT

Downstream 100 bases:

>100_bases
GAAGACGACCCATGACTCGGGAGCTTAGGCTTCCGAGTATTTTTTTGCGAATGCGGGAATTATAATGAAGAGAGGGAAGG
GGGAATTGAGATGGATGGAA

Product: PTS system, glucose subfamily, IIA subunit

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MGFFKKLFGGKEEQLNAVSPLTGKVVDITNVPDQVFSQKMMGDGIAIEPTEGKVVSPVSGTIATVFPTKHAIGINADNGA
EYLIHIGLDTVNLKGEGFETHVTEGQSVKAGDPLVTFDLDFIKANAPSTITPIIITNHDSFGVNKLVAEGDTVTAGSSEV
VELTKK

Sequences:

>Translated_166_residues
MGFFKKLFGGKEEQLNAVSPLTGKVVDITNVPDQVFSQKMMGDGIAIEPTEGKVVSPVSGTIATVFPTKHAIGINADNGA
EYLIHIGLDTVNLKGEGFETHVTEGQSVKAGDPLVTFDLDFIKANAPSTITPIIITNHDSFGVNKLVAEGDTVTAGSSEV
VELTKK
>Mature_165_residues
GFFKKLFGGKEEQLNAVSPLTGKVVDITNVPDQVFSQKMMGDGIAIEPTEGKVVSPVSGTIATVFPTKHAIGINADNGAE
YLIHIGLDTVNLKGEGFETHVTEGQSVKAGDPLVTFDLDFIKANAPSTITPIIITNHDSFGVNKLVAEGDTVTAGSSEVV
ELTKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=171, Percent_Identity=43.2748538011696, Blast_Score=146, Evalue=8e-37,
Organism=Escherichia coli, GI1786894, Length=148, Percent_Identity=45.2702702702703, Blast_Score=136, Evalue=6e-34,
Organism=Escherichia coli, GI1790159, Length=129, Percent_Identity=46.5116279069767, Blast_Score=122, Evalue=9e-30,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17485; Mature: 17354

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFFKKLFGGKEEQLNAVSPLTGKVVDITNVPDQVFSQKMMGDGIAIEPTEGKVVSPVSG
CCCHHHHHCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEEECCCCCEECCCCC
TIATVFPTKHAIGINADNGAEYLIHIGLDTVNLKGEGFETHVTEGQSVKAGDPLVTFDLD
CEEEEECCCEEEEEECCCCCEEEEEECCEEEEECCCCCEEECCCCCCEECCCCEEEEEEE
FIKANAPSTITPIIITNHDSFGVNKLVAEGDTVTAGSSEVVELTKK
EEECCCCCCEEEEEEECCCCCCCEEEEECCCEEECCCCCEEEEECC
>Mature Secondary Structure 
GFFKKLFGGKEEQLNAVSPLTGKVVDITNVPDQVFSQKMMGDGIAIEPTEGKVVSPVSG
CCHHHHHCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEEECCCCCEECCCCC
TIATVFPTKHAIGINADNGAEYLIHIGLDTVNLKGEGFETHVTEGQSVKAGDPLVTFDLD
CEEEEECCCEEEEEECCCCCEEEEEECCEEEEECCCCCEEECCCCCCEECCCCEEEEEEE
FIKANAPSTITPIIITNHDSFGVNKLVAEGDTVTAGSSEVVELTKK
EEECCCCCCEEEEEEECCCCCCCEEEEECCCEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]