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The map label for this gene is yhcK [H]

Identifier: 229916655

GI number: 229916655

Start: 877909

End: 878964

Strand: Reverse

Name: yhcK [H]

Synonym: EAT1b_0927

Alternate gene names: 229916655

Gene position: 878964-877909 (Counterclockwise)

Preceding gene: 229916656

Following gene: 229916654

Centisome position: 29.3

GC content: 52.75

Gene sequence:

>1056_bases
GTGGGAATCGTCATTAACTATTTTGTGATCAACATCAGTCTTATCTTTTTATTCATGTCGCTCACACTCTATGTGATGCG
GACGGTCTTACCGATTCAGCCGACCTCCCCGTTATTTGTACGATTTTGGTTTGGGGTGTCAAACGGGTTGACGGCCATCC
TGTTGACGATCAATGCGATTGAACTCGGTGATGCCCGCATTGACTTACGTCTGATTCCGATCGCCTTAGCCGCTACATTT
GCGGGACCGTTCGGGGCAATCGTCACCCTGGGGCTCATCTTTATCGGTCGGATGACGATTCATGGGATGAGCGCCCCGTT
CATTGACGCCGTCCTCATGTTTGTTGTCTTTTTCTTGTTTTCTCTCGTGATATCACGTGTGCCCATCAGTCGAGGAAGAG
GGTATGCCATCTACATCGGATTTGGTGCACTCCTCGTTTTGATTCGCGTGACCGGAAGTGTGGATATGGACGTGTTTGTG
AACGTATTCGTGCCCTATTTTCTGATGCTGTCACTGGGGGCATGGCTCTGTTATTGGGCCGCGAAGAAGCTCGAGACGCA
CCTGTGGATGTTCCTTCTTCATACGCACCGGGCGACCATCGATGAATTGACGGGACTTCCGAACCGATATCGGACCCTCG
AGCAGATGAACGATATGGAAATGTCCGGGAAGCCATGGACGTTACTCGTCATTGATGTCGATCATTTCAAACAGTTGAAC
GATACATATGGACACTTGGCAGGGGACGCGGCGTTACGTCATATCGGACAGACGCTTGAGCGACATCGTCCACCAGCCGG
ATTTGTCGGCCGCTATGGCGGTGAGGAATTTTTACTCGTCATCGAAGGCGGTGAAGGGATGACGAAGGTGGCGGAAGGCA
TCGTGCAGACGGTACGGCATACGACGTTCGAATTTCAAGGCGAACGCATCCCGATGACCGTCTCCATCGGCGCGGCGATC
GCTGGGCATGAGCCGAGCATGGTCGTGTTTGAGCGGGCCGATGCGGCGTTATACGAAGCAAAACGAAATGGACGGGACCA
GGTCCGGTTCGCATGA

Upstream 100 bases:

>100_bases
AGCGTGAGCTTTCGACTTTTTTCATCATTATGGCACTTGAGGGTGACATTTCTCACCGAGTTGGGATAGGATGGAAACGG
AAAGGGAGGGAGGAGAATCC

Downstream 100 bases:

>100_bases
AGCTATGAATCGTGAGCATACACGATTCATAGCTTTTTTTGGTTTTTATATCGATAAAATCTATATTAATTGAAAAAAAG
ATAAAAATAGACAATATAAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MGIVINYFVINISLIFLFMSLTLYVMRTVLPIQPTSPLFVRFWFGVSNGLTAILLTINAIELGDARIDLRLIPIALAATF
AGPFGAIVTLGLIFIGRMTIHGMSAPFIDAVLMFVVFFLFSLVISRVPISRGRGYAIYIGFGALLVLIRVTGSVDMDVFV
NVFVPYFLMLSLGAWLCYWAAKKLETHLWMFLLHTHRATIDELTGLPNRYRTLEQMNDMEMSGKPWTLLVIDVDHFKQLN
DTYGHLAGDAALRHIGQTLERHRPPAGFVGRYGGEEFLLVIEGGEGMTKVAEGIVQTVRHTTFEFQGERIPMTVSIGAAI
AGHEPSMVVFERADAALYEAKRNGRDQVRFA

Sequences:

>Translated_351_residues
MGIVINYFVINISLIFLFMSLTLYVMRTVLPIQPTSPLFVRFWFGVSNGLTAILLTINAIELGDARIDLRLIPIALAATF
AGPFGAIVTLGLIFIGRMTIHGMSAPFIDAVLMFVVFFLFSLVISRVPISRGRGYAIYIGFGALLVLIRVTGSVDMDVFV
NVFVPYFLMLSLGAWLCYWAAKKLETHLWMFLLHTHRATIDELTGLPNRYRTLEQMNDMEMSGKPWTLLVIDVDHFKQLN
DTYGHLAGDAALRHIGQTLERHRPPAGFVGRYGGEEFLLVIEGGEGMTKVAEGIVQTVRHTTFEFQGERIPMTVSIGAAI
AGHEPSMVVFERADAALYEAKRNGRDQVRFA
>Mature_350_residues
GIVINYFVINISLIFLFMSLTLYVMRTVLPIQPTSPLFVRFWFGVSNGLTAILLTINAIELGDARIDLRLIPIALAATFA
GPFGAIVTLGLIFIGRMTIHGMSAPFIDAVLMFVVFFLFSLVISRVPISRGRGYAIYIGFGALLVLIRVTGSVDMDVFVN
VFVPYFLMLSLGAWLCYWAAKKLETHLWMFLLHTHRATIDELTGLPNRYRTLEQMNDMEMSGKPWTLLVIDVDHFKQLND
TYGHLAGDAALRHIGQTLERHRPPAGFVGRYGGEEFLLVIEGGEGMTKVAEGIVQTVRHTTFEFQGERIPMTVSIGAAIA
GHEPSMVVFERADAALYEAKRNGRDQVRFA

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=39.6341463414634, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=37.5, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=33.9506172839506, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=37.3563218390805, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786584, Length=189, Percent_Identity=30.1587301587302, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=34.375, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=29.1925465838509, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=31.055900621118, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=29.0123456790123, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=29.2993630573248, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=30.2857142857143, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 39153; Mature: 39022

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIVINYFVINISLIFLFMSLTLYVMRTVLPIQPTSPLFVRFWFGVSNGLTAILLTINAI
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEEEE
ELGDARIDLRLIPIALAATFAGPFGAIVTLGLIFIGRMTIHGMSAPFIDAVLMFVVFFLF
EECCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SLVISRVPISRGRGYAIYIGFGALLVLIRVTGSVDMDVFVNVFVPYFLMLSLGAWLCYWA
HHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKKLETHLWMFLLHTHRATIDELTGLPNRYRTLEQMNDMEMSGKPWTLLVIDVDHFKQLN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHH
DTYGHLAGDAALRHIGQTLERHRPPAGFVGRYGGEEFLLVIEGGEGMTKVAEGIVQTVRH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
TTFEFQGERIPMTVSIGAAIAGHEPSMVVFERADAALYEAKRNGRDQVRFA
HHHEECCCEEEEEEEECCEEECCCCCEEEEECCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GIVINYFVINISLIFLFMSLTLYVMRTVLPIQPTSPLFVRFWFGVSNGLTAILLTINAI
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEEEE
ELGDARIDLRLIPIALAATFAGPFGAIVTLGLIFIGRMTIHGMSAPFIDAVLMFVVFFLF
EECCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
SLVISRVPISRGRGYAIYIGFGALLVLIRVTGSVDMDVFVNVFVPYFLMLSLGAWLCYWA
HHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKKLETHLWMFLLHTHRATIDELTGLPNRYRTLEQMNDMEMSGKPWTLLVIDVDHFKQLN
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHHHH
DTYGHLAGDAALRHIGQTLERHRPPAGFVGRYGGEEFLLVIEGGEGMTKVAEGIVQTVRH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
TTFEFQGERIPMTVSIGAAIAGHEPSMVVFERADAALYEAKRNGRDQVRFA
HHHEECCCEEEEEEEECCEEECCCCCEEEEECCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]