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The map label for this gene is prfB

Identifier: 229916623

GI number: 229916623

Start: 850185

End: 851285

Strand: Reverse

Name: prfB

Synonym: EAT1b_0895

Alternate gene names: 229916623

Gene position: 851285-850185 (Counterclockwise)

Preceding gene: 229916624

Following gene: 229916622

Centisome position: 28.38

GC content: 54.04

Gene sequence:

>1101_bases
ATGGAAATTTCAGATATTCGGAACGAACTAGAGGCGATGGCGAAACGCCTCGAGAATTACAGAGCGTCTCTTAACTTGAC
AGAGAAGGAAGCACGCATCGCCGAGCTCGAGAACGAGATGACGTATCCAGACTTCTGGGACAACCAGGAGAAGGCACAAA
AAGTCATCGACGAGTCAAACGGACTCAAAGCGATGGTCGATAAGTATCAAGACCTCGCGAACCAACATGAGGACGGCGAA
GTGACGCTCGAACTCATCAAGGAAGAGCCGGACGCCGAGATGCAAGAAGAGCTCGGGGAATCGATTCAAGCGCTCAAGAA
GGAGTTTGATGACTTCGAACTCGAAATCCTCTTGAACGGACCATATGACGCGAACAACGCCATCCTCGAATTACACCCGG
GTGCGGGCGGTACCGAGTCACAAGACTGGGCATCGATGCTCCTTCGGATGTATCAACGTTTCGCTGACAAACAAGGCTGG
AAAGTCGAGACGGTCGATTACCTCCCGGGTGAAGAGGCGGGCGTCAAGTCGGTCACGCTCCGCATCGTCGGTCATAACGC
GTACGGCTACTTGAAGGCGGAGAAAGGAATCCATCGATTGGTCCGCATTTCGCCGTTCGACTCGTCAGGCCGTCGACACA
CGTCGTTCGTGTCATGTGACGTCATGCCGGAATTCGATGATGACATCGAGATCGAAGTACGGACAGAGGACCTCCGTATC
GACACGTATCGCGCGTCAGGTGCCGGCGGTCAGCACATCAACACGACGGACTCTGCCGTCCGAATCACGCACGTGCCGAC
GGGCGTCGTCGTTTCGTGTCAGACCGAGCGCTCACAGATCAAGAACCGTGAGTCGGCGATGAAGATGTTGAAAGCGAAAT
TGTACCAACGCGAACTCGACGAGCAGCAACGCAAACTCGACGAAATCCGTGGGGAGAAGACGGACATCGGTTGGGGTAGC
CAAATCCGTTCGTACGTCTTCCACCCGTACTCGATGGTCAAAGACCACCGGACGAACTATGAAGTCGGAAACACGAGTGG
GGTCATGGACGGGGATGTTATGCCGTTCATCGATGCCTACCTTCGTAAAACAAATATGTGA

Upstream 100 bases:

>100_bases
TTCGGGTAAGAAGTTCAAGAACTGTCACGGTAGACAACAGGCTTAATTATTGAAGAGGCGGGTCCGCTCGCCTCTTTCCT
ACGGAAAGGTGAATCACACT

Downstream 100 bases:

>100_bases
TGCATGTGGCTCAAAGACACCCGTCTTTGGGCCATGTTGTGTGTAAAGGAGACTTTCTTTAATGAAGCAAATCGTCAAAG
ACTATCTGTATTTGTTGACC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MEISDIRNELEAMAKRLENYRASLNLTEKEARIAELENEMTYPDFWDNQEKAQKVIDESNGLKAMVDKYQDLANQHEDGE
VTLELIKEEPDAEMQEELGESIQALKKEFDDFELEILLNGPYDANNAILELHPGAGGTESQDWASMLLRMYQRFADKQGW
KVETVDYLPGEEAGVKSVTLRIVGHNAYGYLKAEKGIHRLVRISPFDSSGRRHTSFVSCDVMPEFDDDIEIEVRTEDLRI
DTYRASGAGGQHINTTDSAVRITHVPTGVVVSCQTERSQIKNRESAMKMLKAKLYQRELDEQQRKLDEIRGEKTDIGWGS
QIRSYVFHPYSMVKDHRTNYEVGNTSGVMDGDVMPFIDAYLRKTNM

Sequences:

>Translated_366_residues
MEISDIRNELEAMAKRLENYRASLNLTEKEARIAELENEMTYPDFWDNQEKAQKVIDESNGLKAMVDKYQDLANQHEDGE
VTLELIKEEPDAEMQEELGESIQALKKEFDDFELEILLNGPYDANNAILELHPGAGGTESQDWASMLLRMYQRFADKQGW
KVETVDYLPGEEAGVKSVTLRIVGHNAYGYLKAEKGIHRLVRISPFDSSGRRHTSFVSCDVMPEFDDDIEIEVRTEDLRI
DTYRASGAGGQHINTTDSAVRITHVPTGVVVSCQTERSQIKNRESAMKMLKAKLYQRELDEQQRKLDEIRGEKTDIGWGS
QIRSYVFHPYSMVKDHRTNYEVGNTSGVMDGDVMPFIDAYLRKTNM
>Mature_366_residues
MEISDIRNELEAMAKRLENYRASLNLTEKEARIAELENEMTYPDFWDNQEKAQKVIDESNGLKAMVDKYQDLANQHEDGE
VTLELIKEEPDAEMQEELGESIQALKKEFDDFELEILLNGPYDANNAILELHPGAGGTESQDWASMLLRMYQRFADKQGW
KVETVDYLPGEEAGVKSVTLRIVGHNAYGYLKAEKGIHRLVRISPFDSSGRRHTSFVSCDVMPEFDDDIEIEVRTEDLRI
DTYRASGAGGQHINTTDSAVRITHVPTGVVVSCQTERSQIKNRESAMKMLKAKLYQRELDEQQRKLDEIRGEKTDIGWGS
QIRSYVFHPYSMVKDHRTNYEVGNTSGVMDGDVMPFIDAYLRKTNM

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=260, Percent_Identity=41.9230769230769, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI34577120, Length=302, Percent_Identity=35.7615894039735, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI166795305, Length=170, Percent_Identity=41.1764705882353, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI2367172, Length=362, Percent_Identity=47.2375690607735, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI1787462, Length=246, Percent_Identity=42.2764227642276, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=325, Percent_Identity=31.6923076923077, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=302, Percent_Identity=31.7880794701987, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19921226, Length=274, Percent_Identity=37.5912408759124, Blast_Score=167, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_EXISA (C4L5K9)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885269.1
- ProteinModelPortal:   C4L5K9
- GeneID:   7869300
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0895
- OMA:   TEMLLRM
- ProtClustDB:   CLSK2489825
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41860; Mature: 41860

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISDIRNELEAMAKRLENYRASLNLTEKEARIAELENEMTYPDFWDNQEKAQKVIDESN
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GLKAMVDKYQDLANQHEDGEVTLELIKEEPDAEMQEELGESIQALKKEFDDFELEILLNG
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
PYDANNAILELHPGAGGTESQDWASMLLRMYQRFADKQGWKVETVDYLPGEEAGVKSVTL
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEE
RIVGHNAYGYLKAEKGIHRLVRISPFDSSGRRHTSFVSCDVMPEFDDDIEIEVRTEDLRI
EEEECCCEEEEEHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCEEE
DTYRASGAGGQHINTTDSAVRITHVPTGVVVSCQTERSQIKNRESAMKMLKAKLYQRELD
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EQQRKLDEIRGEKTDIGWGSQIRSYVFHPYSMVKDHRTNYEVGNTSGVMDGDVMPFIDAY
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LRKTNM
HHHCCC
>Mature Secondary Structure
MEISDIRNELEAMAKRLENYRASLNLTEKEARIAELENEMTYPDFWDNQEKAQKVIDESN
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GLKAMVDKYQDLANQHEDGEVTLELIKEEPDAEMQEELGESIQALKKEFDDFELEILLNG
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
PYDANNAILELHPGAGGTESQDWASMLLRMYQRFADKQGWKVETVDYLPGEEAGVKSVTL
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEE
RIVGHNAYGYLKAEKGIHRLVRISPFDSSGRRHTSFVSCDVMPEFDDDIEIEVRTEDLRI
EEEECCCEEEEEHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCEEE
DTYRASGAGGQHINTTDSAVRITHVPTGVVVSCQTERSQIKNRESAMKMLKAKLYQRELD
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EQQRKLDEIRGEKTDIGWGSQIRSYVFHPYSMVKDHRTNYEVGNTSGVMDGDVMPFIDAY
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LRKTNM
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA