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The map label for this gene is rpoN [H]

Identifier: 229916595

GI number: 229916595

Start: 820378

End: 821523

Strand: Reverse

Name: rpoN [H]

Synonym: EAT1b_0867

Alternate gene names: 229916595

Gene position: 821523-820378 (Counterclockwise)

Preceding gene: 229916597

Following gene: 229916594

Centisome position: 27.39

GC content: 53.75

Gene sequence:

>1146_bases
ATGCAACGGCAGGAGCAAAAACAAATTCAGCAACAACAGATGACACAAGACCTCGTGCAGCGGTTACCGGTCCTTGAAGC
GGATCGTCTCGCGCTAGTATTCCGGTTGGCGCGTCTTGCACGACATAACCCGAACATTCACATCGGGAGCGGGGGCGTCG
ATTGGATTGCAGATGCGGCAAGCTCGTTTGAAGAGAAGCTGTTCACACAGATTCATGAGGGGAGCGGCAGTGAGTCTGAG
AAAGCGTTGCGACGACAACTCGTCCTCTTGCTCGACCATCGGGGATTATTGCCGGAGGCGAATGAAGTGTTGGCGGAGCG
ACTCGGTGTCGACGTCACTCAGGTCGAACAGGCGAGGCAGACACTCTTATTTCTAGAACCGACGGGGATCGGGTCAAGAA
CGGAACGGGAGTGTCAATATCGACATGCGATCGAACATGAGGACCCTCGCGTCGTCGATGTATTCGAAGCGATCGAGAGT
GGGGTGAGTCCTCAACAGATGACGACTGCTCTCGCTATTTCAAATGAGGAATTGGCAAGGCTGGAACGAATCGTCGCGAC
ACTTCCGAAACGTCCTCTATTATATGAAGAAACGGGTCATGTTTTTCCGGAACTCGAAGTACGCTATGAAGAGGGGACAC
TCGTCACGACGTGGCTCAGTCCAGAACTCGACGAAACGCGTGTCGGGAACGACCGAGAGGCGAAATGGTGGATGACGTCC
CTTTCGATGCGGAACGAGACGCTGCGTGAGATCTGGTCGGTCATCGAATCACGTCAGACTGAGTGGCTCCAAGGGGAACT
CGTTCTCCATCCCTTGACCCGGCGGACGGTCGCACAGCTCATAGGAAAACATGAATCGACGGTCGGACGAGCCATCCAAG
GGAAGTTCGCCAAGACCGAGAGTGGCATCATGGAATGGCGACATTTTTTTGTTCGGTCTACAAGTCGCGGGCATTCGCCG
TTTATGATTAAACGGGCACTCGCAGAACTGATTCAACGAGAGAAGGCACCACAAAGTGATCAGGCGCTTGCGAATGAATT
GATTCGATACGGTTATCAAGTGACCCGGCGGACGGTGACGAACTATCGAGAAGAGCTTGGATTCGGTAATTCGCGCGAGC
GTGAGCGACAGTTATGGAAGGGATGA

Upstream 100 bases:

>100_bases
TATTATACATTTGTTTTATTAAAAAAGCAATCCTGTTCGCATAGCTTTTTTGAGCGCGACGGAAGCGGGTTCGTTACAAT
AGAAAAGAGGGGGGAGAACG

Downstream 100 bases:

>100_bases
CGAAAATGAAATTAACATTATATAAACGCGACAATTGTTCACTATGTGACGAGGCGGTCGTCATGCTCGAATGGTTACAG
GAAGATTATCCGATCGAACT

Product: sigma-54 DNA-binding domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MQRQEQKQIQQQQMTQDLVQRLPVLEADRLALVFRLARLARHNPNIHIGSGGVDWIADAASSFEEKLFTQIHEGSGSESE
KALRRQLVLLLDHRGLLPEANEVLAERLGVDVTQVEQARQTLLFLEPTGIGSRTERECQYRHAIEHEDPRVVDVFEAIES
GVSPQQMTTALAISNEELARLERIVATLPKRPLLYEETGHVFPELEVRYEEGTLVTTWLSPELDETRVGNDREAKWWMTS
LSMRNETLREIWSVIESRQTEWLQGELVLHPLTRRTVAQLIGKHESTVGRAIQGKFAKTESGIMEWRHFFVRSTSRGHSP
FMIKRALAELIQREKAPQSDQALANELIRYGYQVTRRTVTNYREELGFGNSRERERQLWKG

Sequences:

>Translated_381_residues
MQRQEQKQIQQQQMTQDLVQRLPVLEADRLALVFRLARLARHNPNIHIGSGGVDWIADAASSFEEKLFTQIHEGSGSESE
KALRRQLVLLLDHRGLLPEANEVLAERLGVDVTQVEQARQTLLFLEPTGIGSRTERECQYRHAIEHEDPRVVDVFEAIES
GVSPQQMTTALAISNEELARLERIVATLPKRPLLYEETGHVFPELEVRYEEGTLVTTWLSPELDETRVGNDREAKWWMTS
LSMRNETLREIWSVIESRQTEWLQGELVLHPLTRRTVAQLIGKHESTVGRAIQGKFAKTESGIMEWRHFFVRSTSRGHSP
FMIKRALAELIQREKAPQSDQALANELIRYGYQVTRRTVTNYREELGFGNSRERERQLWKG
>Mature_381_residues
MQRQEQKQIQQQQMTQDLVQRLPVLEADRLALVFRLARLARHNPNIHIGSGGVDWIADAASSFEEKLFTQIHEGSGSESE
KALRRQLVLLLDHRGLLPEANEVLAERLGVDVTQVEQARQTLLFLEPTGIGSRTERECQYRHAIEHEDPRVVDVFEAIES
GVSPQQMTTALAISNEELARLERIVATLPKRPLLYEETGHVFPELEVRYEEGTLVTTWLSPELDETRVGNDREAKWWMTS
LSMRNETLREIWSVIESRQTEWLQGELVLHPLTRRTVAQLIGKHESTVGRAIQGKFAKTESGIMEWRHFFVRSTSRGHSP
FMIKRALAELIQREKAPQSDQALANELIRYGYQVTRRTVTNYREELGFGNSRERERQLWKG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=379, Percent_Identity=24.5382585751979, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 43947; Mature: 43947

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRQEQKQIQQQQMTQDLVQRLPVLEADRLALVFRLARLARHNPNIHIGSGGVDWIADAA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHH
SSFEEKLFTQIHEGSGSESEKALRRQLVLLLDHRGLLPEANEVLAERLGVDVTQVEQARQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
TLLFLEPTGIGSRTERECQYRHAIEHEDPRVVDVFEAIESGVSPQQMTTALAISNEELAR
HEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
LERIVATLPKRPLLYEETGHVFPELEVRYEEGTLVTTWLSPELDETRVGNDREAKWWMTS
HHHHHHHCCCCCCEECCCCCCCCCEEEEEECCCEEEEECCCCCCHHCCCCCCCHHHHHHH
LSMRNETLREIWSVIESRQTEWLQGELVLHPLTRRTVAQLIGKHESTVGRAIQGKFAKTE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHCCHHHHHHHHHHCCHHHHH
SGIMEWRHFFVRSTSRGHSPFMIKRALAELIQREKAPQSDQALANELIRYGYQVTRRTVT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
NYREELGFGNSRERERQLWKG
HHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure
MQRQEQKQIQQQQMTQDLVQRLPVLEADRLALVFRLARLARHNPNIHIGSGGVDWIADAA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHH
SSFEEKLFTQIHEGSGSESEKALRRQLVLLLDHRGLLPEANEVLAERLGVDVTQVEQARQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHH
TLLFLEPTGIGSRTERECQYRHAIEHEDPRVVDVFEAIESGVSPQQMTTALAISNEELAR
HEEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
LERIVATLPKRPLLYEETGHVFPELEVRYEEGTLVTTWLSPELDETRVGNDREAKWWMTS
HHHHHHHCCCCCCEECCCCCCCCCEEEEEECCCEEEEECCCCCCHHCCCCCCCHHHHHHH
LSMRNETLREIWSVIESRQTEWLQGELVLHPLTRRTVAQLIGKHESTVGRAIQGKFAKTE
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHCCHHHHHHHHHHCCHHHHH
SGIMEWRHFFVRSTSRGHSPFMIKRALAELIQREKAPQSDQALANELIRYGYQVTRRTVT
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
NYREELGFGNSRERERQLWKG
HHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]