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The map label for this gene is est [H]

Identifier: 229916581

GI number: 229916581

Start: 806200

End: 806940

Strand: Reverse

Name: est [H]

Synonym: EAT1b_0853

Alternate gene names: 229916581

Gene position: 806940-806200 (Counterclockwise)

Preceding gene: 229916582

Following gene: 229916580

Centisome position: 26.9

GC content: 52.9

Gene sequence:

>741_bases
ATGAAAGTGACAGCACCAAAACCGTTCTTCTTCGAGGGCGGACAACGTGCCGTGTTGATGTTACATGGCTTCACTGGATC
GAGCGCGGATGTCCGCATGCTCGGTCGCTTCCTTCAAAAAGAAGGATACACATGTATGGGACCGATCTATCGTGGTCATG
CCGTCCCACCGGAAGAATTACTCCAATATGGACCGAAAGACTGGTGGGAAGACGTCTTGGACGCCTACCAGACACTCGTC
GACAAAGGCCATGAGGAAATCGTCGTCTGCGGACTCTCGCTAGGTGGCGTGATGTCGCTCCGTCTCGCCGAGGAGAAAGA
CGTCAAGGCGGTCATCCCGATGTGCGCACCGACGGGAATCGATGCCGAGGACCGCTTGTATAAAGGAGTCCAAGCATATG
CCCGAGAATATAAGGGTAGAGAAGAAAAGTCGCCGGAACAGATCGAGCAGGAAATGGCAGAGTTCAAACCGATGCCAACA
TTGAAAGACCTCCGTGCCTTCGTCCGCGACACGGCGTCGAAGCTCGAGGACATCTTCGTCCCGACACTCGTCGTCCAAGG
CGCCAAAGACAACATGATCGACCCGGAATCGGCGAATCAAATTTACGAGGCGGTCAACACGTTCCAAAAAGAGTTGCTCA
TCTATGAGAACTCGGGACACGTGATCACACTCGACAAAGAAAAAGAGAAACTGCACCAGGACGTGCTCGATTTTCTCGAA
ACGTTAGACTGGTCAGTCTAA

Upstream 100 bases:

>100_bases
ATAAGGCTGATCGTTCTCGATTTTCACGTGAGGCGGTCAGCTTTTGTTTTTTTGATTTATTTTTTGGGAATAATGTAGAT
ACGAGGAGATGACAGATCAA

Downstream 100 bases:

>100_bases
GAAGGAGGTGTCACGTTGAATTTACGTGACCAATTACTAGAGATCTTGCAGGCGAGCGAAAAACCGCTCTCTGTGGATCA
ACTGACCGAGCGACTACAGC

Product: carboxylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKVTAPKPFFFEGGQRAVLMLHGFTGSSADVRMLGRFLQKEGYTCMGPIYRGHAVPPEELLQYGPKDWWEDVLDAYQTLV
DKGHEEIVVCGLSLGGVMSLRLAEEKDVKAVIPMCAPTGIDAEDRLYKGVQAYAREYKGREEKSPEQIEQEMAEFKPMPT
LKDLRAFVRDTASKLEDIFVPTLVVQGAKDNMIDPESANQIYEAVNTFQKELLIYENSGHVITLDKEKEKLHQDVLDFLE
TLDWSV

Sequences:

>Translated_246_residues
MKVTAPKPFFFEGGQRAVLMLHGFTGSSADVRMLGRFLQKEGYTCMGPIYRGHAVPPEELLQYGPKDWWEDVLDAYQTLV
DKGHEEIVVCGLSLGGVMSLRLAEEKDVKAVIPMCAPTGIDAEDRLYKGVQAYAREYKGREEKSPEQIEQEMAEFKPMPT
LKDLRAFVRDTASKLEDIFVPTLVVQGAKDNMIDPESANQIYEAVNTFQKELLIYENSGHVITLDKEKEKLHQDVLDFLE
TLDWSV
>Mature_246_residues
MKVTAPKPFFFEGGQRAVLMLHGFTGSSADVRMLGRFLQKEGYTCMGPIYRGHAVPPEELLQYGPKDWWEDVLDAYQTLV
DKGHEEIVVCGLSLGGVMSLRLAEEKDVKAVIPMCAPTGIDAEDRLYKGVQAYAREYKGREEKSPEQIEQEMAEFKPMPT
LKDLRAFVRDTASKLEDIFVPTLVVQGAKDNMIDPESANQIYEAVNTFQKELLIYENSGHVITLDKEKEKLHQDVLDFLE
TLDWSV

Specific function: Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones [H]

COG id: COG1647

COG function: function code R; Esterase/lipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012354
- InterPro:   IPR001375 [H]

Pfam domain/function: PF00326 Peptidase_S9 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 27836; Mature: 27836

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTAPKPFFFEGGQRAVLMLHGFTGSSADVRMLGRFLQKEGYTCMGPIYRGHAVPPEEL
CCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
LQYGPKDWWEDVLDAYQTLVDKGHEEIVVCGLSLGGVMSLRLAEEKDVKAVIPMCAPTGI
HHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCCHHEECCCCCCCC
DAEDRLYKGVQAYAREYKGREEKSPEQIEQEMAEFKPMPTLKDLRAFVRDTASKLEDIFV
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
PTLVVQGAKDNMIDPESANQIYEAVNTFQKELLIYENSGHVITLDKEKEKLHQDVLDFLE
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEECCHHHHHHHHHHHHHH
TLDWSV
HHCCCC
>Mature Secondary Structure
MKVTAPKPFFFEGGQRAVLMLHGFTGSSADVRMLGRFLQKEGYTCMGPIYRGHAVPPEEL
CCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
LQYGPKDWWEDVLDAYQTLVDKGHEEIVVCGLSLGGVMSLRLAEEKDVKAVIPMCAPTGI
HHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCCHHEECCCCCCCC
DAEDRLYKGVQAYAREYKGREEKSPEQIEQEMAEFKPMPTLKDLRAFVRDTASKLEDIFV
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
PTLVVQGAKDNMIDPESANQIYEAVNTFQKELLIYENSGHVITLDKEKEKLHQDVLDFLE
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEECCHHHHHHHHHHHHHH
TLDWSV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1369099 [H]