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The map label for this gene is yfkN [H]

Identifier: 229916554

GI number: 229916554

Start: 779408

End: 782161

Strand: Reverse

Name: yfkN [H]

Synonym: EAT1b_0825

Alternate gene names: 229916554

Gene position: 782161-779408 (Counterclockwise)

Preceding gene: 229916555

Following gene: 229916553

Centisome position: 26.07

GC content: 51.13

Gene sequence:

>2754_bases
ATGAAAACCGCATTGAAATGGTCGATCGCGACCGCGCTCGTGTTACCAACCGTTTTGCCGCTACAGGCACCGAACGTCTT
GGCAGAAGTGGACGAGGATATCGTCAAGCTCCGTTTCTTAGAGACGACTGACTTACATACGAACATCACGAACTACGACT
ACTTCCAAGACAAGGTCGATAACACGATCGGATTGACGAAAGTCGCGACACTCATCAATGAACATCGTACAGCCGCTGGG
GAAACGAACACATTCTTGTTCGATAACGGGGATACGATTCAGGGGACACCGTTTGGTGATTACGTCCGTGAGAGCCAAGC
TGGGAAGAATGAAGCGTTCGAACATCCGATGTACAAGGCGATGGCGGCACTCAACTACGATGCCGTCACACTCGGGAACC
ATGAATTCAACTTCGGTCTCGAATTCTTATACAACGCGATGGAAGGGTCGAAAGGGAAACTTCGTTTCGTCAACTCGAAC
GTCAAAGATTTAGAAGGCAACCCGATTGTCTCGAAGTTCAACGTGGATGGACAAGAAGTCCAAATCATTGAACGAACGGT
CACAGACATGGATGGGGAAACGCATACGATCAAAGTCGGCGTATTCGGAGTCGTCCCTCCGAAAATCATGTCATGGGATT
CGGGGAACCTTCAAGGCCGTGTGACGGCAGAAGATATCATCCCGTCCGCACGTGAAGCGGTTCAAAAATTGAAAGACCGG
GGAGCGGACGTCGTCGTCGCCCTCGCGCACTCAGGCATCGGTGACAGTGCGGCGCTCGCGGACGAACAACTCGACAGCGA
AGAGAACGTCGGATACGCGCTCACGAAGATTGACGGACTCGACATGGTCATGACGGGGCACCAACACGGTCGCTTCCCGG
ATGCGAAAGGCATGTTCGCCTCATTCCCGAACGTCGACATGACAAAAGGGACGATCAATGGCAAACCGGTCGTCATGGCG
AACAACCAAGGGAAGGACCTCGGCGTTATCGACTTCGAGCTTGAACTCGTAGAAGGGAAATGGCAAATCGTCGATGCGTC
AGCAGCACTCGATACGGTGAAGGAAGATACAGCGGTCGACCCGACGATTCAAGCGTTGATCGAAGACGATCACTTGGCGA
CGATCGACTACATCAACCAACCGGTCGGTGAGATTCAAGATGACATCCAAAGCTACTTCGCACTCGTCCGTGACGACGCA
TCGGTTCAGTTCGTGACGAACGCACAGAAGTGGTATGTCGAGAAAGAGCTTGAAACGAACCCAGAACTTTCTGCATATAA
AGAGTTGCCGCTCTTGTCAGCGGGTGCACCGTTCAAAGCAGGTGGACGTGAATTGACGGATGCAGGTTACTACACGTATA
TTTCAAAAGGACCAATCGCTTTGAAAAACGTCGCGGACCTTTACGTGTACTCGAATACACTTGAAATCGTCAAATTGACA
GGGAAAGATGTCATGGACTGGCTTGAAATGTCTGCAGGGGCATACAATCAAATTGATGCAGACGGCGAAAACTTACTCAA
CCTTCAATTCCGTAGCTACAACTTCGATGTTCTCGACGGTTTGACGTATGAAATCGATGTCACGGGTCCTGCGAAGTTCG
ATGTCGGCGGAAAGCTCGTCAACGAGAACGCGAACCGCGTGAAAAACGTCGAGTACAATGGACAACCGATCAAGATGGAC
CAAGAGTTCTTAGTCGCGACGAACAACTATCGCGCAGGTTCTTCAACATTCCCAGGCCTTGGTGGCGGGAAGAACATCGT
CTATCGCTCAGCGTATGAGACACGTAACGTCATCTCGGACTACATTGTCGAAAGTAAAGACAAGTTGGACTACAAAGCCG
ACAACAACTGGAAGATTGTCGCAGACGCGAAGCGTTCGGTGACATTTGAGACAGCGGACGCAGGGAAGCAGTACTTGAAC
CTTTACGAAGGAATCGAAGCGACGGACGCGACGCGTCAAGATGCGGCAGGTCGTCTCTTCCGCACTTATACGACGGAGCT
CGAGGCAGCACCAGTTGATTTGACGCTTTCGGTCGATGTCATCAAACCAGGTGCGAAAGTCGTGACCGGGAAGACGGCGC
CAAATGCGAACGTGACATTGAAAGATGGCCAAACGGTTCTCGCCACGACGACAGCCGATGCACAAGGGATGTACCGTTTG
ACGGTCAAGCCACTCAAACTTCGTCAGTCGTTGACGATTGAAAGTGAATTGAACGGGAAGACGATGACGGAAACGAAAGT
CGTCGGAGACGGATTTGTCGGAGCGCCTGCCATCTCGAAACGTTTGACGTATGCCAACACGGCAGTACTGACGGGAACGT
CTTCGCCAGGTGCGAAAATGGTGTTGAAGCGGGACGGAAAACGAATCGCCTATGCGACGGCAGACGAATCTGGTAAATTC
GAATTCACGAAACGTGCTGGTTTCGTTGCGGCAAGCTATGAAATCGAAGCGAAAGATGTTTCTGGTAAAGTGGTTGAATC
CGAGTCATTTGTGATCGAGAAACCTAAGATGGTGACGCCGAAGAACACGAAAGGCGTGAAAGCGGGTACGCGTGCCTTGA
TCGGGAAAACGACACCAGGCGCGACGGTCGTCTTGAAAGACGCCAAAGGTAAGCAAGTCGCGAAAGTGAAGGCGAGCTGG
TCTGGTACGTATACGTTCACATTCAAAAAAGGCCTTGCGAAAGGGACGTACAAAGTCATCGCGAGTGACTACGATGGTTC
TGGCGCAAAACAATCAACTTTTAACTTGAAATAA

Upstream 100 bases:

>100_bases
TAGGTCTATTTCTATACAAAAATTTAATAGGAGGGAAAGACAGCTGGTGTTATATTGAATATGGTTAGACAATTATCAAC
CAGAAAAAGGGGACAATCAC

Downstream 100 bases:

>100_bases
GTACAGAACGACGTCCGAATCGGGCGTCGTTTTTTGTCGAATAAAAGTGTCTAAAATGTGAATGAAAGCCCTTACATTAT
CATCCGGTCTTATGTATAAT

Product: 5'-Nucleotidase domain protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 917; Mature: 917

Protein sequence:

>917_residues
MKTALKWSIATALVLPTVLPLQAPNVLAEVDEDIVKLRFLETTDLHTNITNYDYFQDKVDNTIGLTKVATLINEHRTAAG
ETNTFLFDNGDTIQGTPFGDYVRESQAGKNEAFEHPMYKAMAALNYDAVTLGNHEFNFGLEFLYNAMEGSKGKLRFVNSN
VKDLEGNPIVSKFNVDGQEVQIIERTVTDMDGETHTIKVGVFGVVPPKIMSWDSGNLQGRVTAEDIIPSAREAVQKLKDR
GADVVVALAHSGIGDSAALADEQLDSEENVGYALTKIDGLDMVMTGHQHGRFPDAKGMFASFPNVDMTKGTINGKPVVMA
NNQGKDLGVIDFELELVEGKWQIVDASAALDTVKEDTAVDPTIQALIEDDHLATIDYINQPVGEIQDDIQSYFALVRDDA
SVQFVTNAQKWYVEKELETNPELSAYKELPLLSAGAPFKAGGRELTDAGYYTYISKGPIALKNVADLYVYSNTLEIVKLT
GKDVMDWLEMSAGAYNQIDADGENLLNLQFRSYNFDVLDGLTYEIDVTGPAKFDVGGKLVNENANRVKNVEYNGQPIKMD
QEFLVATNNYRAGSSTFPGLGGGKNIVYRSAYETRNVISDYIVESKDKLDYKADNNWKIVADAKRSVTFETADAGKQYLN
LYEGIEATDATRQDAAGRLFRTYTTELEAAPVDLTLSVDVIKPGAKVVTGKTAPNANVTLKDGQTVLATTTADAQGMYRL
TVKPLKLRQSLTIESELNGKTMTETKVVGDGFVGAPAISKRLTYANTAVLTGTSSPGAKMVLKRDGKRIAYATADESGKF
EFTKRAGFVAASYEIEAKDVSGKVVESESFVIEKPKMVTPKNTKGVKAGTRALIGKTTPGATVVLKDAKGKQVAKVKASW
SGTYTFTFKKGLAKGTYKVIASDYDGSGAKQSTFNLK

Sequences:

>Translated_917_residues
MKTALKWSIATALVLPTVLPLQAPNVLAEVDEDIVKLRFLETTDLHTNITNYDYFQDKVDNTIGLTKVATLINEHRTAAG
ETNTFLFDNGDTIQGTPFGDYVRESQAGKNEAFEHPMYKAMAALNYDAVTLGNHEFNFGLEFLYNAMEGSKGKLRFVNSN
VKDLEGNPIVSKFNVDGQEVQIIERTVTDMDGETHTIKVGVFGVVPPKIMSWDSGNLQGRVTAEDIIPSAREAVQKLKDR
GADVVVALAHSGIGDSAALADEQLDSEENVGYALTKIDGLDMVMTGHQHGRFPDAKGMFASFPNVDMTKGTINGKPVVMA
NNQGKDLGVIDFELELVEGKWQIVDASAALDTVKEDTAVDPTIQALIEDDHLATIDYINQPVGEIQDDIQSYFALVRDDA
SVQFVTNAQKWYVEKELETNPELSAYKELPLLSAGAPFKAGGRELTDAGYYTYISKGPIALKNVADLYVYSNTLEIVKLT
GKDVMDWLEMSAGAYNQIDADGENLLNLQFRSYNFDVLDGLTYEIDVTGPAKFDVGGKLVNENANRVKNVEYNGQPIKMD
QEFLVATNNYRAGSSTFPGLGGGKNIVYRSAYETRNVISDYIVESKDKLDYKADNNWKIVADAKRSVTFETADAGKQYLN
LYEGIEATDATRQDAAGRLFRTYTTELEAAPVDLTLSVDVIKPGAKVVTGKTAPNANVTLKDGQTVLATTTADAQGMYRL
TVKPLKLRQSLTIESELNGKTMTETKVVGDGFVGAPAISKRLTYANTAVLTGTSSPGAKMVLKRDGKRIAYATADESGKF
EFTKRAGFVAASYEIEAKDVSGKVVESESFVIEKPKMVTPKNTKGVKAGTRALIGKTTPGATVVLKDAKGKQVAKVKASW
SGTYTFTFKKGLAKGTYKVIASDYDGSGAKQSTFNLK
>Mature_917_residues
MKTALKWSIATALVLPTVLPLQAPNVLAEVDEDIVKLRFLETTDLHTNITNYDYFQDKVDNTIGLTKVATLINEHRTAAG
ETNTFLFDNGDTIQGTPFGDYVRESQAGKNEAFEHPMYKAMAALNYDAVTLGNHEFNFGLEFLYNAMEGSKGKLRFVNSN
VKDLEGNPIVSKFNVDGQEVQIIERTVTDMDGETHTIKVGVFGVVPPKIMSWDSGNLQGRVTAEDIIPSAREAVQKLKDR
GADVVVALAHSGIGDSAALADEQLDSEENVGYALTKIDGLDMVMTGHQHGRFPDAKGMFASFPNVDMTKGTINGKPVVMA
NNQGKDLGVIDFELELVEGKWQIVDASAALDTVKEDTAVDPTIQALIEDDHLATIDYINQPVGEIQDDIQSYFALVRDDA
SVQFVTNAQKWYVEKELETNPELSAYKELPLLSAGAPFKAGGRELTDAGYYTYISKGPIALKNVADLYVYSNTLEIVKLT
GKDVMDWLEMSAGAYNQIDADGENLLNLQFRSYNFDVLDGLTYEIDVTGPAKFDVGGKLVNENANRVKNVEYNGQPIKMD
QEFLVATNNYRAGSSTFPGLGGGKNIVYRSAYETRNVISDYIVESKDKLDYKADNNWKIVADAKRSVTFETADAGKQYLN
LYEGIEATDATRQDAAGRLFRTYTTELEAAPVDLTLSVDVIKPGAKVVTGKTAPNANVTLKDGQTVLATTTADAQGMYRL
TVKPLKLRQSLTIESELNGKTMTETKVVGDGFVGAPAISKRLTYANTAVLTGTSSPGAKMVLKRDGKRIAYATADESGKF
EFTKRAGFVAASYEIEAKDVSGKVVESESFVIEKPKMVTPKNTKGVKAGTRALIGKTTPGATVVLKDAKGKQVAKVKASW
SGTYTFTFKKGLAKGTYKVIASDYDGSGAKQSTFNLK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=633, Percent_Identity=42.4960505529226, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 99818; Mature: 99818

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALKWSIATALVLPTVLPLQAPNVLAEVDEDIVKLRFLETTDLHTNITNYDYFQDKVD
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEEEEECCCCCCCCCCHHHHHHHC
NTIGLTKVATLINEHRTAAGETNTFLFDNGDTIQGTPFGDYVRESQAGKNEAFEHPMYKA
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEECCCCCHHHHHHHCCCCCHHHCCHHHHH
MAALNYDAVTLGNHEFNFGLEFLYNAMEGSKGKLRFVNSNVKDLEGNPIVSKFNVDGQEV
HHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHE
QIIERTVTDMDGETHTIKVGVFGVVPPKIMSWDSGNLQGRVTAEDIIPSAREAVQKLKDR
EEEEHHHHCCCCCEEEEEEEEEECCCCHHEECCCCCCEEEEEHHHHCCHHHHHHHHHHHC
GADVVVALAHSGIGDSAALADEQLDSEENVGYALTKIDGLDMVMTGHQHGRFPDAKGMFA
CCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCEE
SFPNVDMTKGTINGKPVVMANNQGKDLGVIDFELELVEGKWQIVDASAALDTVKEDTAVD
CCCCCCEEECCCCCCEEEEECCCCCEEEEEEEEEEEECCEEEEEECHHHHHHHHCCCCCC
PTIQALIEDDHLATIDYINQPVGEIQDDIQSYFALVRDDASVQFVTNAQKWYVEKELETN
HHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCC
PELSAYKELPLLSAGAPFKAGGRELTDAGYYTYISKGPIALKNVADLYVYSNTLEIVKLT
CCHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCEEECCCEEEEEEECCEEEEEEC
GKDVMDWLEMSAGAYNQIDADGENLLNLQFRSYNFDVLDGLTYEIDVTGPAKFDVGGKLV
CHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEECCEEEEEEECCCCEECCCCEEE
NENANRVKNVEYNGQPIKMDQEFLVATNNYRAGSSTFPGLGGGKNIVYRSAYETRNVISD
CCCCCCEECCEECCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHH
YIVESKDKLDYKADNNWKIVADAKRSVTFETADAGKQYLNLYEGIEATDATRQDAAGRLF
HHHCCCCCCCEECCCCEEEEEECCCEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHCCCEE
RTYTTELEAAPVDLTLSVDVIKPGAKVVTGKTAPNANVTLKDGQTVLATTTADAQGMYRL
EEEECCCCCCCEEEEEEEEEECCCCEEEECCCCCCCCEEEECCCEEEEEECCCCCCEEEE
TVKPLKLRQSLTIESELNGKTMTETKVVGDGFVGAPAISKRLTYANTAVLTGTSSPGAKM
EECEEEHHCEEEEECCCCCCEEEEEEEECCCCCCCCHHHHCEEEECEEEEECCCCCCCEE
VLKRDGKRIAYATADESGKFEFTKRAGFVAASYEIEAKDVSGKVVESESFVIEKPKMVTP
EEEECCCEEEEEECCCCCCEEEEECCCEEEEEEEEEECCCCCEEEECCCEEEECCCEECC
KNTKGVKAGTRALIGKTTPGATVVLKDAKGKQVAKVKASWSGTYTFTFKKGLAKGTYKVI
CCCCCCCCCCCEEEECCCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCEEEEE
ASDYDGSGAKQSTFNLK
EECCCCCCCCCCEECCC
>Mature Secondary Structure
MKTALKWSIATALVLPTVLPLQAPNVLAEVDEDIVKLRFLETTDLHTNITNYDYFQDKVD
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEEEEEEECCCCCCCCCCHHHHHHHC
NTIGLTKVATLINEHRTAAGETNTFLFDNGDTIQGTPFGDYVRESQAGKNEAFEHPMYKA
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEECCCCCHHHHHHHCCCCCHHHCCHHHHH
MAALNYDAVTLGNHEFNFGLEFLYNAMEGSKGKLRFVNSNVKDLEGNPIVSKFNVDGQEV
HHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHE
QIIERTVTDMDGETHTIKVGVFGVVPPKIMSWDSGNLQGRVTAEDIIPSAREAVQKLKDR
EEEEHHHHCCCCCEEEEEEEEEECCCCHHEECCCCCCEEEEEHHHHCCHHHHHHHHHHHC
GADVVVALAHSGIGDSAALADEQLDSEENVGYALTKIDGLDMVMTGHQHGRFPDAKGMFA
CCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCEE
SFPNVDMTKGTINGKPVVMANNQGKDLGVIDFELELVEGKWQIVDASAALDTVKEDTAVD
CCCCCCEEECCCCCCEEEEECCCCCEEEEEEEEEEEECCEEEEEECHHHHHHHHCCCCCC
PTIQALIEDDHLATIDYINQPVGEIQDDIQSYFALVRDDASVQFVTNAQKWYVEKELETN
HHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCC
PELSAYKELPLLSAGAPFKAGGRELTDAGYYTYISKGPIALKNVADLYVYSNTLEIVKLT
CCHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCEEECCCEEEEEEECCEEEEEEC
GKDVMDWLEMSAGAYNQIDADGENLLNLQFRSYNFDVLDGLTYEIDVTGPAKFDVGGKLV
CHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEEECCEEEEEEECCCCEECCCCEEE
NENANRVKNVEYNGQPIKMDQEFLVATNNYRAGSSTFPGLGGGKNIVYRSAYETRNVISD
CCCCCCEECCEECCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHH
YIVESKDKLDYKADNNWKIVADAKRSVTFETADAGKQYLNLYEGIEATDATRQDAAGRLF
HHHCCCCCCCEECCCCEEEEEECCCEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHCCCEE
RTYTTELEAAPVDLTLSVDVIKPGAKVVTGKTAPNANVTLKDGQTVLATTTADAQGMYRL
EEEECCCCCCCEEEEEEEEEECCCCEEEECCCCCCCCEEEECCCEEEEEECCCCCCEEEE
TVKPLKLRQSLTIESELNGKTMTETKVVGDGFVGAPAISKRLTYANTAVLTGTSSPGAKM
EECEEEHHCEEEEECCCCCCEEEEEEEECCCCCCCCHHHHCEEEECEEEEECCCCCCCEE
VLKRDGKRIAYATADESGKFEFTKRAGFVAASYEIEAKDVSGKVVESESFVIEKPKMVTP
EEEECCCEEEEEECCCCCCEEEEECCCEEEEEEEEEECCCCCEEEECCCEEEECCCEECC
KNTKGVKAGTRALIGKTTPGATVVLKDAKGKQVAKVKASWSGTYTFTFKKGLAKGTYKVI
CCCCCCCCCCCEEEECCCCCCEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCEEEEE
ASDYDGSGAKQSTFNLK
EECCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]