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The map label for this gene is ldh2 [H]

Identifier: 229916526

GI number: 229916526

Start: 750779

End: 751762

Strand: Reverse

Name: ldh2 [H]

Synonym: EAT1b_0796

Alternate gene names: 229916526

Gene position: 751762-750779 (Counterclockwise)

Preceding gene: 229916527

Following gene: 229916524

Centisome position: 25.06

GC content: 52.13

Gene sequence:

>984_bases
ATGTCACGTATTCCGCATAAAGATGAGATTGACCCGAAAGACATCACTCGCATCGCCGTCATCGGTGCCGGTTGGGTCGG
GGTCAGTTTTGCTTATCAATTATCGACGGCTGCCCTTTGTGAGGAGCTCGTCTTGATCGACTCGAATCATGCCAAGGCAG
AAGGGGAGGCGATGGACCTCAATCATGGGATCTCATTCGCCCCGACACCGGTCCGGATTTGGGCAGGCGACTACTCGGAT
TGTAAAGACGCAGATATCGTCGTCATCACGGCAGGAGCCGCACAAAAACTTGGGCAGACACGGATGGACCTCGTCGAGCA
AAATGCAAAGGTCGTCCGCACCGTCACACAAGACCTGATGAAGAGTGGATTTGACGGCATCTTAGTCGTCGCGACGAACC
CGGTCGATGTGATGGCACACGTCGCTTGGAAAGCATCAGGTTTACCAAAAGAACGTGTCATCGGTTCAGGAACGGTCCTC
GATACAGCACGACTCCGTTACAAACTCGGAGACTATTTCGAATTGTCGCCACGAAACTGCCACGCTTATTATATGGGGGA
ACATGGCGATACGGGATTTGTCGCATGGAACAACGCTCGTATCTATGGGAAGAGTATCGATGAACTGTTAGCAGAGAACG
AACAGTATCAGTTCGAGGACCTCGAAGCGATTTATACTGACGTGCGCGACGCCGCTTATCAGATTATCGAGTATAAGAGT
GCCGCCTATTATGCGATTGGGGTCGGATTGCTTCGCATCATCCGGGCCATCGTGCGAAATGAAAACTCCGTCGTGACGAT
TGGTGCTCATTTAGACGGCGAGTACGGGGCGAGTGACGTGCATATCGGCGTACCGGCCTTGATCGACCGGAGCGGTATTC
GTAAGATCATCGAGATTGAATTGACGGAGGAAGAACAACAGAAGTTTGACGCCTCGGTACAAGCCATCAAGGCGGGAATC
GAGAAAGTCGAACCTGCATCCTAA

Upstream 100 bases:

>100_bases
TATAACGGAATCACGTATTTTTCATGCAGCTTTTGTCGTTAAAGATGGAAGAGTCGGGTATGCTAAAAAAGAGAATGAAT
CATGGAAAGGATGGATACAC

Downstream 100 bases:

>100_bases
AAAAGATCCGACTGCCTAAGCAGTCGGATTGTTGGTTAAGACGCCTCTTCTTCGGCGATATCTTTCTTATAGTACTTCTC
GGATGTGAAGAATTCGGTCG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2 [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSRIPHKDEIDPKDITRIAVIGAGWVGVSFAYQLSTAALCEELVLIDSNHAKAEGEAMDLNHGISFAPTPVRIWAGDYSD
CKDADIVVITAGAAQKLGQTRMDLVEQNAKVVRTVTQDLMKSGFDGILVVATNPVDVMAHVAWKASGLPKERVIGSGTVL
DTARLRYKLGDYFELSPRNCHAYYMGEHGDTGFVAWNNARIYGKSIDELLAENEQYQFEDLEAIYTDVRDAAYQIIEYKS
AAYYAIGVGLLRIIRAIVRNENSVVTIGAHLDGEYGASDVHIGVPALIDRSGIRKIIEIELTEEEQQKFDASVQAIKAGI
EKVEPAS

Sequences:

>Translated_327_residues
MSRIPHKDEIDPKDITRIAVIGAGWVGVSFAYQLSTAALCEELVLIDSNHAKAEGEAMDLNHGISFAPTPVRIWAGDYSD
CKDADIVVITAGAAQKLGQTRMDLVEQNAKVVRTVTQDLMKSGFDGILVVATNPVDVMAHVAWKASGLPKERVIGSGTVL
DTARLRYKLGDYFELSPRNCHAYYMGEHGDTGFVAWNNARIYGKSIDELLAENEQYQFEDLEAIYTDVRDAAYQIIEYKS
AAYYAIGVGLLRIIRAIVRNENSVVTIGAHLDGEYGASDVHIGVPALIDRSGIRKIIEIELTEEEQQKFDASVQAIKAGI
EKVEPAS
>Mature_326_residues
SRIPHKDEIDPKDITRIAVIGAGWVGVSFAYQLSTAALCEELVLIDSNHAKAEGEAMDLNHGISFAPTPVRIWAGDYSDC
KDADIVVITAGAAQKLGQTRMDLVEQNAKVVRTVTQDLMKSGFDGILVVATNPVDVMAHVAWKASGLPKERVIGSGTVLD
TARLRYKLGDYFELSPRNCHAYYMGEHGDTGFVAWNNARIYGKSIDELLAENEQYQFEDLEAIYTDVRDAAYQIIEYKSA
AYYAIGVGLLRIIRAIVRNENSVVTIGAHLDGEYGASDVHIGVPALIDRSGIRKIIEIELTEEEQQKFDASVQAIKAGIE
KVEPAS

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI260099723, Length=318, Percent_Identity=33.9622641509434, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI5031857, Length=318, Percent_Identity=33.9622641509434, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=33.6633663366337, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=33.6633663366337, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI47059044, Length=303, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI221136809, Length=303, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=31.9218241042345, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=33.0128205128205, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=33.0128205128205, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI260099727, Length=203, Percent_Identity=38.9162561576355, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI260099725, Length=248, Percent_Identity=34.6774193548387, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=32.8205128205128, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI103472011, Length=308, Percent_Identity=24.6753246753247, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI103472015, Length=208, Percent_Identity=26.9230769230769, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI21735621, Length=329, Percent_Identity=25.531914893617, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789632, Length=321, Percent_Identity=27.1028037383178, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=35.6164383561644, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17554310, Length=328, Percent_Identity=26.8292682926829, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17136226, Length=320, Percent_Identity=33.125, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=28.4345047923323, Blast_Score=150, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24647881, Length=327, Percent_Identity=24.4648318042813, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 35847; Mature: 35716

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIPHKDEIDPKDITRIAVIGAGWVGVSFAYQLSTAALCEELVLIDSNHAKAEGEAMDL
CCCCCCCCCCCHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEC
NHGISFAPTPVRIWAGDYSDCKDADIVVITAGAAQKLGQTRMDLVEQNAKVVRTVTQDLM
CCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSGFDGILVVATNPVDVMAHVAWKASGLPKERVIGSGTVLDTARLRYKLGDYFELSPRNC
HCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCEEHHHHHHHHCCCEEEECCCCE
HAYYMGEHGDTGFVAWNNARIYGKSIDELLAENEQYQFEDLEAIYTDVRDAAYQIIEYKS
EEEEECCCCCCCEEEECCCEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AAYYAIGVGLLRIIRAIVRNENSVVTIGAHLDGEYGASDVHIGVPALIDRSGIRKIIEIE
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCHHHHCHHCCCEEEEEE
LTEEEQQKFDASVQAIKAGIEKVEPAS
ECCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRIPHKDEIDPKDITRIAVIGAGWVGVSFAYQLSTAALCEELVLIDSNHAKAEGEAMDL
CCCCCCCCCCHHHCEEEEEEECCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEC
NHGISFAPTPVRIWAGDYSDCKDADIVVITAGAAQKLGQTRMDLVEQNAKVVRTVTQDLM
CCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSGFDGILVVATNPVDVMAHVAWKASGLPKERVIGSGTVLDTARLRYKLGDYFELSPRNC
HCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCEEHHHHHHHHCCCEEEECCCCE
HAYYMGEHGDTGFVAWNNARIYGKSIDELLAENEQYQFEDLEAIYTDVRDAAYQIIEYKS
EEEEECCCCCCCEEEECCCEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AAYYAIGVGLLRIIRAIVRNENSVVTIGAHLDGEYGASDVHIGVPALIDRSGIRKIIEIE
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCHHHHCHHCCCEEEEEE
LTEEEQQKFDASVQAIKAGIEKVEPAS
ECCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]