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The map label for this gene is yutF [H]

Identifier: 229916514

GI number: 229916514

Start: 739905

End: 740684

Strand: Reverse

Name: yutF [H]

Synonym: EAT1b_0784

Alternate gene names: 229916514

Gene position: 740684-739905 (Counterclockwise)

Preceding gene: 229916515

Following gene: 229916512

Centisome position: 24.69

GC content: 49.23

Gene sequence:

>780_bases
ATGGAACCGATAAAAGGATATTTATTTGATTTGGATGGTACGATGTATGCCGGAACAGAGCCCGTTCAAGCGGCAGTTGA
ATTCGTCAACGAGTTAGAAGCGACGGGCGTGCCATACTTATTCGTCACGAATAACGCCTCGATGACACAACAGCAAATTG
CAGATAAGTTGATTTCAATGGGGGCAAATGTGAAGGCAGAAAACGTGTTGACGAGTGCCATGGCGACGGCTTTTTATATT
GAGAAGATGTCACCGGGCGCGACGGTCTATATGATTGGCGAAGACGGACTCCGCCTCGCCCTAGAATCCCGTGGGTTCCA
CGTCACGGATGAACCGAAAGCAGACTATGTGGTCATCGGTCTCGACCGTCACATCACGTATGAGAAGTTGGCGAGAGGAG
CAATCGCCATTCGTTCAGGAGCCCGATTCATTTCGACGAATGGGGATATCGCCATTCCGACAGAACGTGGGTTCTTACCG
GGGAATGGGTCGCTCACATCCGTGTTGACGGTGACGACCGAGAAAGAACCGTTCTTCATTGGGAAGCCGGAACCGGTCAT
GATTGACATCGCACTCGACATGATTGGATTGACGAAAGAAGATGTCGTGATGGTAGGGGATAACTATCATACGGATATTC
TGTTCGGAATCAACGGGGGAATCCGTACACTTCACGTCAACTCAGGTGTACACGGTCCAGCCTTTGTTCAGGAACAAGAA
AAACTCCCGACCTATATGGTCGAGAGTTTAGATGAATGGAAGCATGAAAAAAAGGGATGA

Upstream 100 bases:

>100_bases
TCAAAACAAGACTTCACAGCGTTCTCGACACAAGTTCGTCGCTACTTAGAGGTCGAACTTGGACCAGTGTCCGCATTCTT
ACCAGAAAGCGAGTGATTGG

Downstream 100 bases:

>100_bases
CGAATGTCTCCCTTTTTACTTAGAATACTTGTTCTACTTCGACGATGCCTGGTACTTCTTCGATGAGCGCGCGCTCAATA
CCAGCTTTTAGCGTGATTGT

Product: HAD-superfamily subfamily IIA hydrolase like protein

Products: 4-nitrophenol; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MEPIKGYLFDLDGTMYAGTEPVQAAVEFVNELEATGVPYLFVTNNASMTQQQIADKLISMGANVKAENVLTSAMATAFYI
EKMSPGATVYMIGEDGLRLALESRGFHVTDEPKADYVVIGLDRHITYEKLARGAIAIRSGARFISTNGDIAIPTERGFLP
GNGSLTSVLTVTTEKEPFFIGKPEPVMIDIALDMIGLTKEDVVMVGDNYHTDILFGINGGIRTLHVNSGVHGPAFVQEQE
KLPTYMVESLDEWKHEKKG

Sequences:

>Translated_259_residues
MEPIKGYLFDLDGTMYAGTEPVQAAVEFVNELEATGVPYLFVTNNASMTQQQIADKLISMGANVKAENVLTSAMATAFYI
EKMSPGATVYMIGEDGLRLALESRGFHVTDEPKADYVVIGLDRHITYEKLARGAIAIRSGARFISTNGDIAIPTERGFLP
GNGSLTSVLTVTTEKEPFFIGKPEPVMIDIALDMIGLTKEDVVMVGDNYHTDILFGINGGIRTLHVNSGVHGPAFVQEQE
KLPTYMVESLDEWKHEKKG
>Mature_259_residues
MEPIKGYLFDLDGTMYAGTEPVQAAVEFVNELEATGVPYLFVTNNASMTQQQIADKLISMGANVKAENVLTSAMATAFYI
EKMSPGATVYMIGEDGLRLALESRGFHVTDEPKADYVVIGLDRHITYEKLARGAIAIRSGARFISTNGDIAIPTERGFLP
GNGSLTSVLTVTTEKEPFFIGKPEPVMIDIALDMIGLTKEDVVMVGDNYHTDILFGINGGIRTLHVNSGVHGPAFVQEQE
KLPTYMVESLDEWKHEKKG

Specific function: Unknown

COG id: COG0647

COG function: function code G; Predicted sugar phosphatases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI10092677, Length=268, Percent_Identity=28.3582089552239, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI108796653, Length=281, Percent_Identity=29.1814946619217, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI14149777, Length=244, Percent_Identity=25.4098360655738, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786890, Length=249, Percent_Identity=33.7349397590361, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI193210059, Length=256, Percent_Identity=29.296875, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI86563050, Length=245, Percent_Identity=29.3877551020408, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558880, Length=260, Percent_Identity=24.6153846153846, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17562458, Length=260, Percent_Identity=24.6153846153846, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17560956, Length=260, Percent_Identity=24.6153846153846, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71984613, Length=259, Percent_Identity=25.8687258687259, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319965, Length=237, Percent_Identity=29.1139240506329, Blast_Score=108, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24666141, Length=243, Percent_Identity=28.8065843621399, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24656326, Length=251, Percent_Identity=28.2868525896414, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24656330, Length=254, Percent_Identity=27.1653543307087, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI22026920, Length=235, Percent_Identity=24.6808510638298, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19920940, Length=255, Percent_Identity=27.843137254902, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24666137, Length=273, Percent_Identity=25.6410256410256, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI18859765, Length=242, Percent_Identity=24.3801652892562, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006357
- InterPro:   IPR006354
- InterPro:   IPR023215 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.41

Molecular weight: Translated: 28266; Mature: 28266

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIKGYLFDLDGTMYAGTEPVQAAVEFVNELEATGVPYLFVTNNASMTQQQIADKLISM
CCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHC
GANVKAENVLTSAMATAFYIEKMSPGATVYMIGEDGLRLALESRGFHVTDEPKADYVVIG
CCCCCHHHHHHHHHHHHHEEEECCCCCEEEEECCCCEEEEEECCCEEECCCCCCCEEEEE
LDRHITYEKLARGAIAIRSGARFISTNGDIAIPTERGFLPGNGSLTSVLTVTTEKEPFFI
CCCCCCHHHHHCCEEEEECCCEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECCCCEEE
GKPEPVMIDIALDMIGLTKEDVVMVGDNYHTDILFGINGGIRTLHVNSGVHGPAFVQEQE
CCCCCEEEEEEEEECCCCCCCEEEECCCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHH
KLPTYMVESLDEWKHEKKG
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPIKGYLFDLDGTMYAGTEPVQAAVEFVNELEATGVPYLFVTNNASMTQQQIADKLISM
CCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHC
GANVKAENVLTSAMATAFYIEKMSPGATVYMIGEDGLRLALESRGFHVTDEPKADYVVIG
CCCCCHHHHHHHHHHHHHEEEECCCCCEEEEECCCCEEEEEECCCEEECCCCCCCEEEEE
LDRHITYEKLARGAIAIRSGARFISTNGDIAIPTERGFLPGNGSLTSVLTVTTEKEPFFI
CCCCCCHHHHHCCEEEEECCCEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECCCCEEE
GKPEPVMIDIALDMIGLTKEDVVMVGDNYHTDILFGINGGIRTLHVNSGVHGPAFVQEQE
CCCCCEEEEEEEEECCCCCCCEEEECCCCCEEEEEEECCCEEEEEECCCCCCCHHHHHHH
KLPTYMVESLDEWKHEKKG
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-nitrophenyl phosphate; H2O

Specific reaction: 4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]