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The map label for this gene is metK [H]

Identifier: 229916472

GI number: 229916472

Start: 695073

End: 696281

Strand: Reverse

Name: metK [H]

Synonym: EAT1b_0742

Alternate gene names: 229916472

Gene position: 696281-695073 (Counterclockwise)

Preceding gene: 229916474

Following gene: 229916470

Centisome position: 23.21

GC content: 49.63

Gene sequence:

>1209_bases
ATGTCAAAATTGAACCGTCGACTTTTTACTTCAGAGTCGGTTACTGAAGGACATCCAGACAAAATTTGTGATCAGATTTC
TGACTCCATCTTGGATGCCATTTTAGCGGAAGACCCGAATGCCCGTGTTGCGGCAGAAACGTCTGTCACAACAGGTCTTG
TACTCGTAGCTGGTGAAATCACAACGTCAACTTACGTGGATATCCCGAAAGTTGTCCGTGAAACAATCCGCGAAATCGGA
TACACACGTGCGAAATACGGCTTTGATGCTGAAACGTGTGCGGTCTTGACGTCGATTGATGAGCAATCGGTAGACATCGC
GCAAGGTGTTGACCAGGCGCTTGAAGCACGTGAAGGTCAGATGACTGAAGAGGAACTCGATGCAATCGGTGCAGGAGACC
AAGGTCTCATGTTCGGATATGCAACAAACGAGACACCTGAACTCATGCCGCTTCCGATCTCGTTGGCACACAAGTTGTCA
CGTCGTCTTGCTGAAGTACGTAAGAACGGTACGCTCGAGTACCTTCGTCCGGACGGGAAGACGCAAGTAACGGTTGAGTA
TGATGAGGACAACAACCCAGTTCGCATCGATACGATCGTTATTTCGACACAACACGCGGAAGAGATCACGCTTGAGCAAA
TTCAAGAAGACTTGAAAAAACATGTCATCGAGGCAGTCATTCCGGCTGAACTCATCGATGCTGAAACAAAATACTTCATC
AACCCGACTGGTCGCTTCGTAATCGGTGGACCACAAGGAGATGCAGGTTTGACAGGACGTAAGATCATCGTTGATACGTA
CGGTGGATACGCTCGTCACGGTGGCGGCGCATTCTCTGGTAAAGATCCTACAAAAGTTGACCGTTCAGCGGCATACGCAG
CTCGCTATGTAGCGAAAAACATCGTTGCAGCAGGACTTGCGGATAAAGCGGAAGTTCAACTTGCGTACGCAATCGGTGTT
GCACACCCAGTATCGATCGCTGTCGATACGTTCGGCACAGGTAAATTGAGCGAAACGGAGCTCGTTGAACTCGTTCGTGA
GAACTTCGACCTCCGTCCGGCAGGCATCATCAAGATGCTCGACCTTCGTCGCCCAATCTACAAACAAACGGCAGCATACG
GTCACTTCGGTCGTACAGACATCGAGCTCCCATGGGAGCAAACAGATAAAGCAGCCATTCTTGAAGAAGGCGCGAAACGT
TTCGCGTAA

Upstream 100 bases:

>100_bases
TATGTCATCGGGGGAGGTTTTTTTCATTACCGTCCCAAAGCAACGCGTAAGGGGAACGAGTACCGTCCACATAATACCCT
TATTATAGGAGGTTTTTTGA

Downstream 100 bases:

>100_bases
TCATCAAAAACGACCTTTCCGCGGAAAGGTCGTTTTTTTACGTTGTCGGAGGTGTCTGTTTATTTGAATCGGATGAAGAA
TCAGTTGTCGTCGTATCTTG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSKLNRRLFTSESVTEGHPDKICDQISDSILDAILAEDPNARVAAETSVTTGLVLVAGEITTSTYVDIPKVVRETIREIG
YTRAKYGFDAETCAVLTSIDEQSVDIAQGVDQALEAREGQMTEEELDAIGAGDQGLMFGYATNETPELMPLPISLAHKLS
RRLAEVRKNGTLEYLRPDGKTQVTVEYDEDNNPVRIDTIVISTQHAEEITLEQIQEDLKKHVIEAVIPAELIDAETKYFI
NPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKAEVQLAYAIGV
AHPVSIAVDTFGTGKLSETELVELVRENFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDIELPWEQTDKAAILEEGAKR
FA

Sequences:

>Translated_402_residues
MSKLNRRLFTSESVTEGHPDKICDQISDSILDAILAEDPNARVAAETSVTTGLVLVAGEITTSTYVDIPKVVRETIREIG
YTRAKYGFDAETCAVLTSIDEQSVDIAQGVDQALEAREGQMTEEELDAIGAGDQGLMFGYATNETPELMPLPISLAHKLS
RRLAEVRKNGTLEYLRPDGKTQVTVEYDEDNNPVRIDTIVISTQHAEEITLEQIQEDLKKHVIEAVIPAELIDAETKYFI
NPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKAEVQLAYAIGV
AHPVSIAVDTFGTGKLSETELVELVRENFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDIELPWEQTDKAAILEEGAKR
FA
>Mature_401_residues
SKLNRRLFTSESVTEGHPDKICDQISDSILDAILAEDPNARVAAETSVTTGLVLVAGEITTSTYVDIPKVVRETIREIGY
TRAKYGFDAETCAVLTSIDEQSVDIAQGVDQALEAREGQMTEEELDAIGAGDQGLMFGYATNETPELMPLPISLAHKLSR
RLAEVRKNGTLEYLRPDGKTQVTVEYDEDNNPVRIDTIVISTQHAEEITLEQIQEDLKKHVIEAVIPAELIDAETKYFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLADKAEVQLAYAIGVA
HPVSIAVDTFGTGKLSETELVELVRENFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDIELPWEQTDKAAILEEGAKRF
A

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=61.2565445026178, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=60.9947643979058, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=65.0632911392405, Blast_Score=522, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI25145633, Length=396, Percent_Identity=56.8181818181818, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17538498, Length=386, Percent_Identity=57.5129533678757, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17538494, Length=386, Percent_Identity=57.2538860103627, Blast_Score=441, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=55.1813471502591, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32565905, Length=386, Percent_Identity=53.1088082901554, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=55.9171597633136, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145635, Length=344, Percent_Identity=54.6511627906977, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=57.2347266881029, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=54.7619047619048, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=37, Percent_Identity=78.3783783783784, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=60.313315926893, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=58.2697201017812, Blast_Score=456, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45552159, Length=383, Percent_Identity=57.7023498694517, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580529, Length=383, Percent_Identity=57.7023498694517, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580537, Length=383, Percent_Identity=57.7023498694517, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580533, Length=383, Percent_Identity=57.7023498694517, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580535, Length=383, Percent_Identity=57.7023498694517, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580531, Length=383, Percent_Identity=57.7023498694517, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580527, Length=383, Percent_Identity=56.9190600522193, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580525, Length=383, Percent_Identity=56.9190600522193, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580541, Length=371, Percent_Identity=55.7951482479784, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=55.1181102362205, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43827; Mature: 43696

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNRRLFTSESVTEGHPDKICDQISDSILDAILAEDPNARVAAETSVTTGLVLVAGEI
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECC
TTSTYVDIPKVVRETIREIGYTRAKYGFDAETCAVLTSIDEQSVDIAQGVDQALEAREGQ
CCCHHCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
MTEEELDAIGAGDQGLMFGYATNETPELMPLPISLAHKLSRRLAEVRKNGTLEYLRPDGK
CCHHHHHHCCCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCC
TQVTVEYDEDNNPVRIDTIVISTQHAEEITLEQIQEDLKKHVIEAVIPAELIDAETKYFI
EEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVAAGLADKAEVQLAYAIGVAHPVSIAVDTFGTGKLSETELVELVRENFDLRPAGIIKML
HHHHCCCCCHHEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
DLRRPIYKQTAAYGHFGRTDIELPWEQTDKAAILEEGAKRFA
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLNRRLFTSESVTEGHPDKICDQISDSILDAILAEDPNARVAAETSVTTGLVLVAGEI
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECC
TTSTYVDIPKVVRETIREIGYTRAKYGFDAETCAVLTSIDEQSVDIAQGVDQALEAREGQ
CCCHHCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
MTEEELDAIGAGDQGLMFGYATNETPELMPLPISLAHKLSRRLAEVRKNGTLEYLRPDGK
CCHHHHHHCCCCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCC
TQVTVEYDEDNNPVRIDTIVISTQHAEEITLEQIQEDLKKHVIEAVIPAELIDAETKYFI
EEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
NPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVAAGLADKAEVQLAYAIGVAHPVSIAVDTFGTGKLSETELVELVRENFDLRPAGIIKML
HHHHCCCCCHHEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
DLRRPIYKQTAAYGHFGRTDIELPWEQTDKAAILEEGAKRFA
HHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]