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The map label for this gene is leuS [H]

Identifier: 229916464

GI number: 229916464

Start: 686592

End: 688997

Strand: Reverse

Name: leuS [H]

Synonym: EAT1b_0734

Alternate gene names: 229916464

Gene position: 688997-686592 (Counterclockwise)

Preceding gene: 229916465

Following gene: 229916463

Centisome position: 22.97

GC content: 51.91

Gene sequence:

>2406_bases
ATGTTGAGCTATTTTAAACATCAAGAAATCGAGCCGAAGTGGCAAAAGCGTTGGGAAGAGAAAGAAGCATTTAAAACGAC
AGAAGACCCGGAAAAGGATTGTTTCTACGTCCTTGATATGTTCCCGTACCCATCCGGTGCAGGTCTTCACGTCGGTCACC
CGGAAGGTTACACAGCGACGGATATCATCGCTCGTATGAAACGGATGCAAGGATATAACGTCCTTCACCCGATTGGCTGG
GATGCGTTCGGTCTTCCTGCCGAGCAATATGCACTCGACACAGGGAACGACCCGCGTGAATTCACAGCGCGCAACATCGA
GACGTTCCGCCGTCAGTTGAAAGAACTCGGATTCTCATACGATTGGGACCGTGAAATTAGCACGACGGACCCGAAATACT
ATAAATGGACGCAGTGGATCTTCACGCAACTCTTCGAACGTGGTCTCGCATACGTCGATGAAGTCGCGGTCAACTGGTGC
CCGGCTCTCGGTACAGTCCTTGCGAACGAGGAAGTCATCGATGGGTTGTCAGAGCGTGGGAACCACCCGGTCTACCGTGT
CCCAATGAAGCAGTGGGTGCTTCGTATCACAGAATACGCAGAGCGTTTGCTTGAAGATTTGGAAGGTCTCGATTGGCCTG
AGAGCGTGAAAGAGATGCAACGCAACTGGATTGGCAAATCAGTCGGTGCCGAGGTCGACTTTAAAGTCGATGGTCACGAT
AAGATTGTCACGGTATTCACAACACGTCCAGATACGCTTCATGGGGCGACATACGTAACACTCGCACCAGAGCATCCGTT
CACGAAAGTGATCACGACGCCTGAACAATCGACGGCAGTCGAAGCTTACATTGAAGAAGTCTCGAAGAAGACGGACCTTG
AGCGTACAGATCTCGCCAAAGAGAAGACGGGTGTCTTCACGGGCGCATATGCGATCAACCCGGTGAACGGCGAGAAACTT
CCGATTTGGACAGCGGACTACGTTCTCTCGACATATGGGACAGGTGCAGTCATGGCTGTTCCTGGACACGATGAGCGCGA
TTATGAGTTTGCGAAAACGTTCGACCTCCCGATCCGTGAAGTCGTCAAAGGTGGAAACGTGGAAGAGGCGGCTTACGTCG
AAGACGGAGAGCACGTCAACTCTGGTAGCTTGAACGGACTTAATAAAGCCGATGCCATCAAGACGATCATTGAAGAGCTC
GAAGCGAGTGGCACAGGGCGTGCGAAGACGACATACCGTCTTCGTGACTGGTTGTTCAGCCGTCAACGTTACTGGGGCGA
GCCGATTCCGGTCGTCCACATGGAAGACGGAACAATGAAGACGGTACCGGTCGAAGAGCTTCCGCTCGAACTTCCGATCA
TCGATGAAATCAAACCATCTGGTACAGGTGAGTCGCCACTCGCGAACGCAGAAGAGTGGTTGACGTACACAGATCCGGTC
ACGGGGGAAAAAGGTCGTCGCGAGACGAACACGATGCCACAATGGGCAGGTAGCTGCTGGTACTACATCCGTTACATCGA
TCCAGATAACGAAGAGATGATCGCGGATCCTGAGAAACTCAAGCGTTGGCTTCCAGTCGACTTGTATATCGGGGGTACGG
AGCATGCCGTTCTTCACTTGCTCTACGCACGTTTCTGGCATAAAGTCCTCTTTGATATCGGTGTCGTCCCGACGAGCGAA
CCGTTCCAAAAACTCTACAACCAAGGAATGATCCTCGGTGAGAACAACGAGAAGATGTCGAAATCTCGTGGGAACGTTGT
CAACCCGGATGATATCGTGAAGTCGCACGGAGCCGATACACTCCGTCTTTACGAAATGTTCATGGGACCACTTGATGCGG
CCATCGCATGGTCGACGAATGGTCTTGACGGGGCACGTCGCTTCCTCGACCGCGTCTGGCGTTTGTTCGATCAGACGAAC
TTCGCAGACGTGGCACCGACTGCCGACTTTGAACGTACGTATCATCAGACGGTGAAAAAAGTCACGGAGGATTATGAAGC
CCTTCGCTTCAACACGGCAATCTCGCAGTTGATGGTCTTCGTCAATGAAGCGAACAAACAAGAGACGTTGCCGAAATCGC
AAATGGTCGACTTCATCAAGATGCTTTCACCGGTCGCGCCACACTTGTCTGAAGAGTTGTGGGAGAAGATGGGCATGACG
GAAGAGTTGACGTATGCGGCTTGGCCGACATACGATGAGTCAAAACTTGTGAGCGACACGATTGAAGTCGTCATCCAGGT
GAACGGGAAACTCCGTGCGAAAATGCACGTCTCTCGTGACGCATCAAAAGAAGCACTTGAAGCGGAAGCACTCGGGAACG
AGCGTGTCCAAGAGTTCACAGAAGGCAAGACGGTTCGTAAAGTCATTGTCGTTCCTGGAAAACTCGTCAATATTGTAGTG
GGGTAA

Upstream 100 bases:

>100_bases
AAGTGGCAGCAATGCAACATGGGTGGTACCGCGGAGAGCTTTCAAGCCTTTCGTCCCATTTTCGGGGACGGAAGGCTTTT
TATTATGCAGAGGAGGAAGA

Downstream 100 bases:

>100_bases
CAAAAAGAATGTGTGACATTGTCGCACATTCTTTTCTTTTTGGTGAGGTCATTTTCTTTTTTGGACGGTTTTGATACACT
AAAGGGCGATACTGAAGTAG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MLSYFKHQEIEPKWQKRWEEKEAFKTTEDPEKDCFYVLDMFPYPSGAGLHVGHPEGYTATDIIARMKRMQGYNVLHPIGW
DAFGLPAEQYALDTGNDPREFTARNIETFRRQLKELGFSYDWDREISTTDPKYYKWTQWIFTQLFERGLAYVDEVAVNWC
PALGTVLANEEVIDGLSERGNHPVYRVPMKQWVLRITEYAERLLEDLEGLDWPESVKEMQRNWIGKSVGAEVDFKVDGHD
KIVTVFTTRPDTLHGATYVTLAPEHPFTKVITTPEQSTAVEAYIEEVSKKTDLERTDLAKEKTGVFTGAYAINPVNGEKL
PIWTADYVLSTYGTGAVMAVPGHDERDYEFAKTFDLPIREVVKGGNVEEAAYVEDGEHVNSGSLNGLNKADAIKTIIEEL
EASGTGRAKTTYRLRDWLFSRQRYWGEPIPVVHMEDGTMKTVPVEELPLELPIIDEIKPSGTGESPLANAEEWLTYTDPV
TGEKGRRETNTMPQWAGSCWYYIRYIDPDNEEMIADPEKLKRWLPVDLYIGGTEHAVLHLLYARFWHKVLFDIGVVPTSE
PFQKLYNQGMILGENNEKMSKSRGNVVNPDDIVKSHGADTLRLYEMFMGPLDAAIAWSTNGLDGARRFLDRVWRLFDQTN
FADVAPTADFERTYHQTVKKVTEDYEALRFNTAISQLMVFVNEANKQETLPKSQMVDFIKMLSPVAPHLSEELWEKMGMT
EELTYAAWPTYDESKLVSDTIEVVIQVNGKLRAKMHVSRDASKEALEAEALGNERVQEFTEGKTVRKVIVVPGKLVNIVV
G

Sequences:

>Translated_801_residues
MLSYFKHQEIEPKWQKRWEEKEAFKTTEDPEKDCFYVLDMFPYPSGAGLHVGHPEGYTATDIIARMKRMQGYNVLHPIGW
DAFGLPAEQYALDTGNDPREFTARNIETFRRQLKELGFSYDWDREISTTDPKYYKWTQWIFTQLFERGLAYVDEVAVNWC
PALGTVLANEEVIDGLSERGNHPVYRVPMKQWVLRITEYAERLLEDLEGLDWPESVKEMQRNWIGKSVGAEVDFKVDGHD
KIVTVFTTRPDTLHGATYVTLAPEHPFTKVITTPEQSTAVEAYIEEVSKKTDLERTDLAKEKTGVFTGAYAINPVNGEKL
PIWTADYVLSTYGTGAVMAVPGHDERDYEFAKTFDLPIREVVKGGNVEEAAYVEDGEHVNSGSLNGLNKADAIKTIIEEL
EASGTGRAKTTYRLRDWLFSRQRYWGEPIPVVHMEDGTMKTVPVEELPLELPIIDEIKPSGTGESPLANAEEWLTYTDPV
TGEKGRRETNTMPQWAGSCWYYIRYIDPDNEEMIADPEKLKRWLPVDLYIGGTEHAVLHLLYARFWHKVLFDIGVVPTSE
PFQKLYNQGMILGENNEKMSKSRGNVVNPDDIVKSHGADTLRLYEMFMGPLDAAIAWSTNGLDGARRFLDRVWRLFDQTN
FADVAPTADFERTYHQTVKKVTEDYEALRFNTAISQLMVFVNEANKQETLPKSQMVDFIKMLSPVAPHLSEELWEKMGMT
EELTYAAWPTYDESKLVSDTIEVVIQVNGKLRAKMHVSRDASKEALEAEALGNERVQEFTEGKTVRKVIVVPGKLVNIVV
G
>Mature_801_residues
MLSYFKHQEIEPKWQKRWEEKEAFKTTEDPEKDCFYVLDMFPYPSGAGLHVGHPEGYTATDIIARMKRMQGYNVLHPIGW
DAFGLPAEQYALDTGNDPREFTARNIETFRRQLKELGFSYDWDREISTTDPKYYKWTQWIFTQLFERGLAYVDEVAVNWC
PALGTVLANEEVIDGLSERGNHPVYRVPMKQWVLRITEYAERLLEDLEGLDWPESVKEMQRNWIGKSVGAEVDFKVDGHD
KIVTVFTTRPDTLHGATYVTLAPEHPFTKVITTPEQSTAVEAYIEEVSKKTDLERTDLAKEKTGVFTGAYAINPVNGEKL
PIWTADYVLSTYGTGAVMAVPGHDERDYEFAKTFDLPIREVVKGGNVEEAAYVEDGEHVNSGSLNGLNKADAIKTIIEEL
EASGTGRAKTTYRLRDWLFSRQRYWGEPIPVVHMEDGTMKTVPVEELPLELPIIDEIKPSGTGESPLANAEEWLTYTDPV
TGEKGRRETNTMPQWAGSCWYYIRYIDPDNEEMIADPEKLKRWLPVDLYIGGTEHAVLHLLYARFWHKVLFDIGVVPTSE
PFQKLYNQGMILGENNEKMSKSRGNVVNPDDIVKSHGADTLRLYEMFMGPLDAAIAWSTNGLDGARRFLDRVWRLFDQTN
FADVAPTADFERTYHQTVKKVTEDYEALRFNTAISQLMVFVNEANKQETLPKSQMVDFIKMLSPVAPHLSEELWEKMGMT
EELTYAAWPTYDESKLVSDTIEVVIQVNGKLRAKMHVSRDASKEALEAEALGNERVQEFTEGKTVRKVIVVPGKLVNIVV
G

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=878, Percent_Identity=32.5740318906606, Blast_Score=445, Evalue=1e-125,
Organism=Escherichia coli, GI1786861, Length=871, Percent_Identity=43.2835820895522, Blast_Score=693, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=394, Percent_Identity=27.1573604060914, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71997510, Length=774, Percent_Identity=29.8449612403101, Blast_Score=311, Evalue=8e-85,
Organism=Caenorhabditis elegans, GI71997517, Length=774, Percent_Identity=29.8449612403101, Blast_Score=311, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI212645227, Length=321, Percent_Identity=28.3489096573209, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17510661, Length=886, Percent_Identity=21.3318284424379, Blast_Score=114, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323414, Length=874, Percent_Identity=34.5537757437071, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6319395, Length=188, Percent_Identity=28.7234042553192, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355409, Length=891, Percent_Identity=31.8742985409652, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91360; Mature: 91360

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYFKHQEIEPKWQKRWEEKEAFKTTEDPEKDCFYVLDMFPYPSGAGLHVGHPEGYTAT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHH
DIIARMKRMQGYNVLHPIGWDAFGLPAEQYALDTGNDPREFTARNIETFRRQLKELGFSY
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
DWDREISTTDPKYYKWTQWIFTQLFERGLAYVDEVAVNWCPALGTVLANEEVIDGLSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
NHPVYRVPMKQWVLRITEYAERLLEDLEGLDWPESVKEMQRNWIGKSVGAEVDFKVDGHD
CCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCEEEEEECCCC
KIVTVFTTRPDTLHGATYVTLAPEHPFTKVITTPEQSTAVEAYIEEVSKKTDLERTDLAK
EEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHH
EKTGVFTGAYAINPVNGEKLPIWTADYVLSTYGTGAVMAVPGHDERDYEFAKTFDLPIRE
HHCCEEEEEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCHHH
VVKGGNVEEAAYVEDGEHVNSGSLNGLNKADAIKTIIEELEASGTGRAKTTYRLRDWLFS
HHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
RQRYWGEPIPVVHMEDGTMKTVPVEELPLELPIIDEIKPSGTGESPLANAEEWLTYTDPV
HHHHCCCCCCEEEECCCCEEECCHHHCCCCCCCHHCCCCCCCCCCCCCCHHHCEEECCCC
TGEKGRRETNTMPQWAGSCWYYIRYIDPDNEEMIADPEKLKRWLPVDLYIGGTEHAVLHL
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCEEEEECCCHHHHHHH
LYARFWHKVLFDIGVVPTSEPFQKLYNQGMILGENNEKMSKSRGNVVNPDDIVKSHGADT
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEECCCCHHHHHCCCCCCCHHHHHHHCCCCH
LRLYEMFMGPLDAAIAWSTNGLDGARRFLDRVWRLFDQTNFADVAPTADFERTYHQTVKK
HHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
VTEDYEALRFNTAISQLMVFVNEANKQETLPKSQMVDFIKMLSPVAPHLSEELWEKMGMT
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
EELTYAAWPTYDESKLVSDTIEVVIQVNGKLRAKMHVSRDASKEALEAEALGNERVQEFT
HHCEEEECCCCCCHHHHHHHHHEEEEECCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHH
EGKTVRKVIVVPGKLVNIVVG
CCCCEEEEEEECCEEEEEEEC
>Mature Secondary Structure
MLSYFKHQEIEPKWQKRWEEKEAFKTTEDPEKDCFYVLDMFPYPSGAGLHVGHPEGYTAT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHH
DIIARMKRMQGYNVLHPIGWDAFGLPAEQYALDTGNDPREFTARNIETFRRQLKELGFSY
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
DWDREISTTDPKYYKWTQWIFTQLFERGLAYVDEVAVNWCPALGTVLANEEVIDGLSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
NHPVYRVPMKQWVLRITEYAERLLEDLEGLDWPESVKEMQRNWIGKSVGAEVDFKVDGHD
CCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCCCEEEEEECCCC
KIVTVFTTRPDTLHGATYVTLAPEHPFTKVITTPEQSTAVEAYIEEVSKKTDLERTDLAK
EEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHH
EKTGVFTGAYAINPVNGEKLPIWTADYVLSTYGTGAVMAVPGHDERDYEFAKTFDLPIRE
HHCCEEEEEEEECCCCCCCCEEEEHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCHHH
VVKGGNVEEAAYVEDGEHVNSGSLNGLNKADAIKTIIEELEASGTGRAKTTYRLRDWLFS
HHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
RQRYWGEPIPVVHMEDGTMKTVPVEELPLELPIIDEIKPSGTGESPLANAEEWLTYTDPV
HHHHCCCCCCEEEECCCCEEECCHHHCCCCCCCHHCCCCCCCCCCCCCCHHHCEEECCCC
TGEKGRRETNTMPQWAGSCWYYIRYIDPDNEEMIADPEKLKRWLPVDLYIGGTEHAVLHL
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCEEEEECCCHHHHHHH
LYARFWHKVLFDIGVVPTSEPFQKLYNQGMILGENNEKMSKSRGNVVNPDDIVKSHGADT
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEECCCCHHHHHCCCCCCCHHHHHHHCCCCH
LRLYEMFMGPLDAAIAWSTNGLDGARRFLDRVWRLFDQTNFADVAPTADFERTYHQTVKK
HHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
VTEDYEALRFNTAISQLMVFVNEANKQETLPKSQMVDFIKMLSPVAPHLSEELWEKMGMT
HHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
EELTYAAWPTYDESKLVSDTIEVVIQVNGKLRAKMHVSRDASKEALEAEALGNERVQEFT
HHCEEEECCCCCCHHHHHHHHHEEEEECCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHH
EGKTVRKVIVVPGKLVNIVVG
CCCCEEEEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA