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The map label for this gene is amyX [H]

Identifier: 229916451

GI number: 229916451

Start: 672237

End: 674402

Strand: Reverse

Name: amyX [H]

Synonym: EAT1b_0721

Alternate gene names: 229916451

Gene position: 674402-672237 (Counterclockwise)

Preceding gene: 229916452

Following gene: 229916450

Centisome position: 22.48

GC content: 49.86

Gene sequence:

>2166_bases
ATGCATCACACACCAGATCAAAAGACCGCCTCACATGTGACCATCGAGAGTGCGTCTTTCGTATCATTTGAGACCATACA
ACTTGAGATTGTGCGCGAATATGAATCCGTTCCAGTACAAATCGAACAGAATGGACGCCCGGTCGACTACCTCGTCGAGC
AACAGTTTGTGACGGAATCCGGAAAGGTCGAACAAATCCTTCGTTTAAAGGAACCAATCTCTTTAGAAACGTTATACACC
GTCTGCCACCCGGGTCAAAAGACGACGGTTGTCGTACCAAGAGAAATCGTCCGTTCAGAGGAATTCGAACGGCGTTTCTC
ATATGATGGTCCACTCGGCGTATCGTTTACGCCGCACGGAATGGAAGTGACGGTATGGTCTCCGGTTGCTTTATCCATGT
GGATTACGGTACATGATGACACGAATCACCGAGAAATTGAGCGATTTCACATGAATCGCGGCACACGTGGGGAATGGAGT
GGCGAAATACCACAAGCGTTCCAAGGAATGTTGTATCGCATAGAAGTGAAGACACCGGTCGACACGAGGCAAGTCGTTGA
CCCTTATGCGCGAGCCCTGAGTTTGAACGGTGAGTATGGTGTATTGCAAGATGTGGCGCGATTTGTCAAGAAACAGGTCG
AACAAGCCGTGCGCCCCCCTTTATGGAAGGCAACGGATGCCGTCATCTATGAGTTGCACATCCGAGACTTCACAAGCCAC
CCCTCATCGATGAGTCAATTGAAAGGGCTGTATGGTGGGGTGTCTGAAAAAGGAGTTCGGACGAAAGAAGGTAACAGTGC
CGGACTCGACTATTTAAAAGAGCTTGGCGTGACGCATGTCCAGTTACTCCCTGTCCATGATTTTGGAAGTGTCGATGAGG
CGACTCGTATGCCGTACAACTGGGGCTATGACCCGATTCATTGGTTCGGGCTTGAAGGAAGTTATGCCTCAAGACCCGAT
GTGCCGATGAGTCGTGTGTTCGAATATGCGACACTCGTCTCTGACCTGCATCAAGCGGGTCTTCGAGTCGTCACAGACGT
CGTGATGAATCATATTTACATTCGAGAACAATCCCCACTCGAAGCGCTCGTACCATACTATTATTTTCGATACGAGCACG
ACGGGACGATAGCGAACGGGACCGGGGTCGGAAATGACACGGCATCGGAACGCTATATGATGCGACGTATGATTGTGGAC
TTCGTGACGTATTTCGCGACGACGTATCAAGTGGATGGGTTCCGCTTCGATTTGATGGGGATTCATGATGTCGAAACGAT
GAATCAAGTGCGGGCCGCGCTCGATAAGGTGGATCCCTCCATTCTCGTCTATGGAGAAGGGTGGGACTTGGCGACGCCGC
TTGGACAGTCATTGAAGGCAACGTCACAAAATGCTGCACGCATGCCTGGTATCGGACACTTCAACGATATGATTCGAGAT
GCATTAAAAGGGTCAACGTTTAATGACAGGGAACGCGGATTCATCTCTGGCAATGAGTGGAGAGAGTGGGAACTTCGAGA
GTGTCTCACCGGAGCCGTGCATATTCCGAACGTGACGGTCGGACGTTTCCCGAGTCCGGCTTACTCCATCAATTATGCGG
AAGCCCATGACAACTATACGATCTATGACAAGTTAATGTTATCTTGTGAAGGAGTCGATGAGACGACTCGTCTTGCCATG
CAACGTCTTGCCAATGCGGTCGTCATCACATCTCAAGGTATCCCGTTCATCCATGCCGGTCAAGAGTTCGGACGCACGAA
ACATGGTGTCGAGAACAGTTATAATTCACCCGATCACATCAATCATTTTGATTGGGATCTTCGTGACAGACGGATCAAGG
AAATCGAGTATGTGAAGACGCTCTTGAAGTTACGGCGACTCCATACATGTTTCCGCTATGATTCGAGACAAGAAGTGATT
GACCATTTCACGTTTCTCCCATCGCCGCCTGGCGTCATTATCTATGAGCTGACGGCCAGTCATTCTTACGATGCGTGGCA
ACGGGTTCGCGTCTATGTGAACGGTACGTTCGACTCGCTTTCATTTGAGAGCGATGCGAAATGGAACGTTTACGTTCAAG
GAGAAACGGCAAGTCTCGTTCCACTCGCACGAAACCCGGCACACATTCAAGTCGAGTCGTTGTCAATGACGATTCTCGCG
TGTTAA

Upstream 100 bases:

>100_bases
AAAGCACAGCAGATTCGTGACGTTGCCACCGTATTAGAAGTGACTGCCAAGTTACCGTTTCGTCAATTCATGGATCAGTG
ATTGAGAGGAAGGGACGCAT

Downstream 100 bases:

>100_bases
GATGTTGATTGTATCTCTTTAATTCAAGTAAGATTTGAGCGTGATGAATATGTATTCAGATATAGTCGGTGCGCTCGGGA
AAGAGTGGACAGTCGCTTCA

Product: pullulanase, type I

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MHHTPDQKTASHVTIESASFVSFETIQLEIVREYESVPVQIEQNGRPVDYLVEQQFVTESGKVEQILRLKEPISLETLYT
VCHPGQKTTVVVPREIVRSEEFERRFSYDGPLGVSFTPHGMEVTVWSPVALSMWITVHDDTNHREIERFHMNRGTRGEWS
GEIPQAFQGMLYRIEVKTPVDTRQVVDPYARALSLNGEYGVLQDVARFVKKQVEQAVRPPLWKATDAVIYELHIRDFTSH
PSSMSQLKGLYGGVSEKGVRTKEGNSAGLDYLKELGVTHVQLLPVHDFGSVDEATRMPYNWGYDPIHWFGLEGSYASRPD
VPMSRVFEYATLVSDLHQAGLRVVTDVVMNHIYIREQSPLEALVPYYYFRYEHDGTIANGTGVGNDTASERYMMRRMIVD
FVTYFATTYQVDGFRFDLMGIHDVETMNQVRAALDKVDPSILVYGEGWDLATPLGQSLKATSQNAARMPGIGHFNDMIRD
ALKGSTFNDRERGFISGNEWREWELRECLTGAVHIPNVTVGRFPSPAYSINYAEAHDNYTIYDKLMLSCEGVDETTRLAM
QRLANAVVITSQGIPFIHAGQEFGRTKHGVENSYNSPDHINHFDWDLRDRRIKEIEYVKTLLKLRRLHTCFRYDSRQEVI
DHFTFLPSPPGVIIYELTASHSYDAWQRVRVYVNGTFDSLSFESDAKWNVYVQGETASLVPLARNPAHIQVESLSMTILA
C

Sequences:

>Translated_721_residues
MHHTPDQKTASHVTIESASFVSFETIQLEIVREYESVPVQIEQNGRPVDYLVEQQFVTESGKVEQILRLKEPISLETLYT
VCHPGQKTTVVVPREIVRSEEFERRFSYDGPLGVSFTPHGMEVTVWSPVALSMWITVHDDTNHREIERFHMNRGTRGEWS
GEIPQAFQGMLYRIEVKTPVDTRQVVDPYARALSLNGEYGVLQDVARFVKKQVEQAVRPPLWKATDAVIYELHIRDFTSH
PSSMSQLKGLYGGVSEKGVRTKEGNSAGLDYLKELGVTHVQLLPVHDFGSVDEATRMPYNWGYDPIHWFGLEGSYASRPD
VPMSRVFEYATLVSDLHQAGLRVVTDVVMNHIYIREQSPLEALVPYYYFRYEHDGTIANGTGVGNDTASERYMMRRMIVD
FVTYFATTYQVDGFRFDLMGIHDVETMNQVRAALDKVDPSILVYGEGWDLATPLGQSLKATSQNAARMPGIGHFNDMIRD
ALKGSTFNDRERGFISGNEWREWELRECLTGAVHIPNVTVGRFPSPAYSINYAEAHDNYTIYDKLMLSCEGVDETTRLAM
QRLANAVVITSQGIPFIHAGQEFGRTKHGVENSYNSPDHINHFDWDLRDRRIKEIEYVKTLLKLRRLHTCFRYDSRQEVI
DHFTFLPSPPGVIIYELTASHSYDAWQRVRVYVNGTFDSLSFESDAKWNVYVQGETASLVPLARNPAHIQVESLSMTILA
C
>Mature_721_residues
MHHTPDQKTASHVTIESASFVSFETIQLEIVREYESVPVQIEQNGRPVDYLVEQQFVTESGKVEQILRLKEPISLETLYT
VCHPGQKTTVVVPREIVRSEEFERRFSYDGPLGVSFTPHGMEVTVWSPVALSMWITVHDDTNHREIERFHMNRGTRGEWS
GEIPQAFQGMLYRIEVKTPVDTRQVVDPYARALSLNGEYGVLQDVARFVKKQVEQAVRPPLWKATDAVIYELHIRDFTSH
PSSMSQLKGLYGGVSEKGVRTKEGNSAGLDYLKELGVTHVQLLPVHDFGSVDEATRMPYNWGYDPIHWFGLEGSYASRPD
VPMSRVFEYATLVSDLHQAGLRVVTDVVMNHIYIREQSPLEALVPYYYFRYEHDGTIANGTGVGNDTASERYMMRRMIVD
FVTYFATTYQVDGFRFDLMGIHDVETMNQVRAALDKVDPSILVYGEGWDLATPLGQSLKATSQNAARMPGIGHFNDMIRD
ALKGSTFNDRERGFISGNEWREWELRECLTGAVHIPNVTVGRFPSPAYSINYAEAHDNYTIYDKLMLSCEGVDETTRLAM
QRLANAVVITSQGIPFIHAGQEFGRTKHGVENSYNSPDHINHFDWDLRDRRIKEIEYVKTLLKLRRLHTCFRYDSRQEVI
DHFTFLPSPPGVIIYELTASHSYDAWQRVRVYVNGTFDSLSFESDAKWNVYVQGETASLVPLARNPAHIQVESLSMTILA
C

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=596, Percent_Identity=25.8389261744966, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 82222; Mature: 82222

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHTPDQKTASHVTIESASFVSFETIQLEIVREYESVPVQIEQNGRPVDYLVEQQFVTES
CCCCCCCCCCCEEEEECCCEEEEEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHHHHCCC
GKVEQILRLKEPISLETLYTVCHPGQKTTVVVPREIVRSEEFERRFSYDGPLGVSFTPHG
CCHHHHHHHCCCCCHHHHHHEECCCCCEEEECCHHHHCCHHHHHHCCCCCCCCCEECCCC
MEVTVWSPVALSMWITVHDDTNHREIERFHMNRGTRGEWSGEIPQAFQGMLYRIEVKTPV
EEEEEECCEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEEEECCCC
DTRQVVDPYARALSLNGEYGVLQDVARFVKKQVEQAVRPPLWKATDAVIYELHIRDFTSH
CHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCC
PSSMSQLKGLYGGVSEKGVRTKEGNSAGLDYLKELGVTHVQLLPVHDFGSVDEATRMPYN
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCC
WGYDPIHWFGLEGSYASRPDVPMSRVFEYATLVSDLHQAGLRVVTDVVMNHIYIREQSPL
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
EALVPYYYFRYEHDGTIANGTGVGNDTASERYMMRRMIVDFVTYFATTYQVDGFRFDLMG
HHHCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEC
IHDVETMNQVRAALDKVDPSILVYGEGWDLATPLGQSLKATSQNAARMPGIGHFNDMIRD
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHH
ALKGSTFNDRERGFISGNEWREWELRECLTGAVHIPNVTVGRFPSPAYSINYAEAHDNYT
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCEECCCCCCCEEEEEECCCCCEE
IYDKLMLSCEGVDETTRLAMQRLANAVVITSQGIPFIHAGQEFGRTKHGVENSYNSPDHI
EEEEHHHHCCCCCHHHHHHHHHHCCEEEEEECCCCEEECCHHHCCCCCCCCCCCCCCCCC
NHFDWDLRDRRIKEIEYVKTLLKLRRLHTCFRYDSRQEVIDHFTFLPSPPGVIIYELTAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECC
HSYDAWQRVRVYVNGTFDSLSFESDAKWNVYVQGETASLVPLARNPAHIQVESLSMTILA
CCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEEEEEEEEEE
C
C
>Mature Secondary Structure
MHHTPDQKTASHVTIESASFVSFETIQLEIVREYESVPVQIEQNGRPVDYLVEQQFVTES
CCCCCCCCCCCEEEEECCCEEEEEEEEEEEEHHHCCCCEEECCCCCCHHHHHHHHHHCCC
GKVEQILRLKEPISLETLYTVCHPGQKTTVVVPREIVRSEEFERRFSYDGPLGVSFTPHG
CCHHHHHHHCCCCCHHHHHHEECCCCCEEEECCHHHHCCHHHHHHCCCCCCCCCEECCCC
MEVTVWSPVALSMWITVHDDTNHREIERFHMNRGTRGEWSGEIPQAFQGMLYRIEVKTPV
EEEEEECCEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEEEECCCC
DTRQVVDPYARALSLNGEYGVLQDVARFVKKQVEQAVRPPLWKATDAVIYELHIRDFTSH
CHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCC
PSSMSQLKGLYGGVSEKGVRTKEGNSAGLDYLKELGVTHVQLLPVHDFGSVDEATRMPYN
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCC
WGYDPIHWFGLEGSYASRPDVPMSRVFEYATLVSDLHQAGLRVVTDVVMNHIYIREQSPL
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
EALVPYYYFRYEHDGTIANGTGVGNDTASERYMMRRMIVDFVTYFATTYQVDGFRFDLMG
HHHCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEC
IHDVETMNQVRAALDKVDPSILVYGEGWDLATPLGQSLKATSQNAARMPGIGHFNDMIRD
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHH
ALKGSTFNDRERGFISGNEWREWELRECLTGAVHIPNVTVGRFPSPAYSINYAEAHDNYT
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCEECCCCCCCEEEEEECCCCCEE
IYDKLMLSCEGVDETTRLAMQRLANAVVITSQGIPFIHAGQEFGRTKHGVENSYNSPDHI
EEEEHHHHCCCCCHHHHHHHHHHCCEEEEEECCCCEEECCHHHCCCCCCCCCCCCCCCCC
NHFDWDLRDRRIKEIEYVKTLLKLRRLHTCFRYDSRQEVIDHFTFLPSPPGVIIYELTAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEEECC
HSYDAWQRVRVYVNGTFDSLSFESDAKWNVYVQGETASLVPLARNPAHIQVESLSMTILA
CCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEEEEEEEEEE
C
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]