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The map label for this gene is dhaK [H]

Identifier: 229916444

GI number: 229916444

Start: 667859

End: 668836

Strand: Direct

Name: dhaK [H]

Synonym: EAT1b_0714

Alternate gene names: 229916444

Gene position: 667859-668836 (Clockwise)

Preceding gene: 229916443

Following gene: 229916445

Centisome position: 22.26

GC content: 52.76

Gene sequence:

>978_bases
GTGAAGAAGCTAATCGGTGAAGCAAGTGAACTCGTATCGCAAATGGTTGAAGGGATGGTCCATGCCCATCCTGAAATGTA
TGGTCGCGTCGACGGTGTGAATGTCATCTGTCGGGCTGACGGAGCGAAGTCTGGAAAAGTCGGTCTCGTATCTGGAGGCG
GTAGTGGTCACGAGCCGGCCCACGCTGGATATGTCGGGGACGGTATGCTCGATGCGGCAGTATGTGGGGAAGTGTTCACC
TCTCCTACTCCGGACATGGTACTTGAAGGAATCAAAGCAGCTGACGGCGGAAACGGCGTTCTTTTGATTGTGAAAAACTA
TTCCGGCGACGTGATGAACTTTGAGCTCGCCGCAGAACTGGCCGAAGGCGATGGCATCAAAGTCGACCACGTCGTCGTCG
CCGATGATGTCGCCATTTCGAACAAAGAAGATCGACGCGGGGTGGCCGGAACGGTCTTTGTTCACAAAATTGCCGGAGCC
GCTGCCGCTAGTGGCAAATCACTCGAAGAAGTCAAATCCATCGCCGAAAAAGTAGCTTCACACGTTCGCTCCATGGGGAT
GGCCGTAGAACCTTGTTACATGCCGGTCAGTGGTAAACCAGGTTTCGACTTGAACGAGGAAGAGATGGAAATCGGCATCG
GGATTCACGGGGAACGTGGTGTCGAGCGGAAACCGACGAGTGATGTCGACCAAATCGTCGACGACCTCCTCAGTCACTTG
AAAAAAGAAGTCGAGCCAGGAGAAGTTGCCGTCATGGTGAACGGAATGGGCGGAACGCCGTTGTCTGAGTTATACGTGAC
CTATCACTTCGTTGCGGAAAAACTGGAAGCTGCTGGGTATACCCTTAAACGGAAGTATGTCGGCAACTACATGACTTCAC
TCGAGATGCATGGGTTCTCCATCACGCTGCTCCCTCTCGATGATGAGATGACGACATATCTCGACGCTCCTTCGGCAGCA
CTTGGATTCAAAATTTAA

Upstream 100 bases:

>100_bases
TGAAACCGACGAATGATTCGTCGGTTTTTTTGTTTAGTTCCCTCGAAAGAGTGGAAAATTGAAATTGGTAGTTTTCAATT
TAAAAAAGGAGTGATCTCGC

Downstream 100 bases:

>100_bases
TAAATGAGGTGTTTGGTTTATGTTGACGATTGATCGGATGAAGCAGGCGTTACGTGACGCACAAGTTGGCATCGAAGAGC
AAAAGGATACGTTGACCGAT

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKKLIGEASELVSQMVEGMVHAHPEMYGRVDGVNVICRADGAKSGKVGLVSGGGSGHEPAHAGYVGDGMLDAAVCGEVFT
SPTPDMVLEGIKAADGGNGVLLIVKNYSGDVMNFELAAELAEGDGIKVDHVVVADDVAISNKEDRRGVAGTVFVHKIAGA
AAASGKSLEEVKSIAEKVASHVRSMGMAVEPCYMPVSGKPGFDLNEEEMEIGIGIHGERGVERKPTSDVDQIVDDLLSHL
KKEVEPGEVAVMVNGMGGTPLSELYVTYHFVAEKLEAAGYTLKRKYVGNYMTSLEMHGFSITLLPLDDEMTTYLDAPSAA
LGFKI

Sequences:

>Translated_325_residues
MKKLIGEASELVSQMVEGMVHAHPEMYGRVDGVNVICRADGAKSGKVGLVSGGGSGHEPAHAGYVGDGMLDAAVCGEVFT
SPTPDMVLEGIKAADGGNGVLLIVKNYSGDVMNFELAAELAEGDGIKVDHVVVADDVAISNKEDRRGVAGTVFVHKIAGA
AAASGKSLEEVKSIAEKVASHVRSMGMAVEPCYMPVSGKPGFDLNEEEMEIGIGIHGERGVERKPTSDVDQIVDDLLSHL
KKEVEPGEVAVMVNGMGGTPLSELYVTYHFVAEKLEAAGYTLKRKYVGNYMTSLEMHGFSITLLPLDDEMTTYLDAPSAA
LGFKI
>Mature_325_residues
MKKLIGEASELVSQMVEGMVHAHPEMYGRVDGVNVICRADGAKSGKVGLVSGGGSGHEPAHAGYVGDGMLDAAVCGEVFT
SPTPDMVLEGIKAADGGNGVLLIVKNYSGDVMNFELAAELAEGDGIKVDHVVVADDVAISNKEDRRGVAGTVFVHKIAGA
AAASGKSLEEVKSIAEKVASHVRSMGMAVEPCYMPVSGKPGFDLNEEEMEIGIGIHGERGVERKPTSDVDQIVDDLLSHL
KKEVEPGEVAVMVNGMGGTPLSELYVTYHFVAEKLEAAGYTLKRKYVGNYMTSLEMHGFSITLLPLDDEMTTYLDAPSAA
LGFKI

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=335, Percent_Identity=41.1940298507463, Blast_Score=236, Evalue=3e-62,
Organism=Escherichia coli, GI87081857, Length=347, Percent_Identity=44.6685878962536, Blast_Score=296, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17565018, Length=328, Percent_Identity=39.0243902439024, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321055, Length=334, Percent_Identity=38.622754491018, Blast_Score=195, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323570, Length=329, Percent_Identity=36.7781155015198, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34254; Mature: 34254

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIGEASELVSQMVEGMVHAHPEMYGRVDGVNVICRADGAKSGKVGLVSGGGSGHEPA
CCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCC
HAGYVGDGMLDAAVCGEVFTSPTPDMVLEGIKAADGGNGVLLIVKNYSGDVMNFELAAEL
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEEHHHHHHH
AEGDGIKVDHVVVADDVAISNKEDRRGVAGTVFVHKIAGAAAASGKSLEEVKSIAEKVAS
HCCCCEEEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HVRSMGMAVEPCYMPVSGKPGFDLNEEEMEIGIGIHGERGVERKPTSDVDQIVDDLLSHL
HHHHCCCEECCEEECCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
KKEVEPGEVAVMVNGMGGTPLSELYVTYHFVAEKLEAAGYTLKRKYVGNYMTSLEMHGFS
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCEE
ITLLPLDDEMTTYLDAPSAALGFKI
EEEEECCCCHHHHHCCCCHHCCCCC
>Mature Secondary Structure
MKKLIGEASELVSQMVEGMVHAHPEMYGRVDGVNVICRADGAKSGKVGLVSGGGSGHEPA
CCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCC
HAGYVGDGMLDAAVCGEVFTSPTPDMVLEGIKAADGGNGVLLIVKNYSGDVMNFELAAEL
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEEHHHHHHH
AEGDGIKVDHVVVADDVAISNKEDRRGVAGTVFVHKIAGAAAASGKSLEEVKSIAEKVAS
HCCCCEEEEEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
HVRSMGMAVEPCYMPVSGKPGFDLNEEEMEIGIGIHGERGVERKPTSDVDQIVDDLLSHL
HHHHCCCEECCEEECCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
KKEVEPGEVAVMVNGMGGTPLSELYVTYHFVAEKLEAAGYTLKRKYVGNYMTSLEMHGFS
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCEE
ITLLPLDDEMTTYLDAPSAALGFKI
EEEEECCCCHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]