Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 229916347

GI number: 229916347

Start: 569610

End: 570197

Strand: Reverse

Name: grpE [H]

Synonym: EAT1b_0617

Alternate gene names: 229916347

Gene position: 570197-569610 (Counterclockwise)

Preceding gene: 229916348

Following gene: 229916346

Centisome position: 19.01

GC content: 45.58

Gene sequence:

>588_bases
ATGACAGCAGATCACCAAAACGGGCATGAGCAAGATGACGTGCTTGAAGAAAAATCTGTAGACTCGGCACAAACAGAAGA
GAATGAGTCAACTGAATCAAATGAAGATGAGGTTTTAAATGCTGAGATTGTGGAAGAAAGTTCTGAGGGGACGGACTTAC
AAAAAGAAATCGAAGCGTTGAAAGCGAGCGAGCTCCGCATTCGTGCCGACTTTGACAACTTCCGTCGTCGGACGAACGAA
GAGAATGCCAAACGCGTCAAGTTTGCTTCACAGTCTGTCATTGAAAAATTGATTCCGCTCATTGACAACTTTGAGCGTGC
ACTTCAAGTAAACGCAACTTCGGAAGATGCGAAGCAGATTCAATCGGGAGTAGACATGATTCATCGTCAACTACTTGACG
TATTAAATGCTGAGCAAGTCGAAGTGATTGAAGCAGTCGGACAGCCGTTCGACCCGAATTTCCATCAAGCAGTCATGCAA
GAACCGAGCGATGAGTTCGAGTCAGGTATCGTGACGATGGAGCTTCAAAAAGGATATACGATGCATGGTCGAGTCATTCG
CCCGAGCATGGTAAAAGTCGCAGAATAA

Upstream 100 bases:

>100_bases
CAACGCGTATGAATTACCGTCATGGTGTCCAAATGATGGGACAAATTATCGAAGCGTTGCGACGAACGAAACTAGATGAG
TGACGGAAAGGAGTTTTACT

Downstream 100 bases:

>100_bases
AACTACTTAACGAGACGGAGGAATAAACGATGGGTAAAATTATCGGTATTGACTTAGGGACTACAAACTCATGTGTGGCA
GTAATGGAAGGTGGCGAAGC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MTADHQNGHEQDDVLEEKSVDSAQTEENESTESNEDEVLNAEIVEESSEGTDLQKEIEALKASELRIRADFDNFRRRTNE
ENAKRVKFASQSVIEKLIPLIDNFERALQVNATSEDAKQIQSGVDMIHRQLLDVLNAEQVEVIEAVGQPFDPNFHQAVMQ
EPSDEFESGIVTMELQKGYTMHGRVIRPSMVKVAE

Sequences:

>Translated_195_residues
MTADHQNGHEQDDVLEEKSVDSAQTEENESTESNEDEVLNAEIVEESSEGTDLQKEIEALKASELRIRADFDNFRRRTNE
ENAKRVKFASQSVIEKLIPLIDNFERALQVNATSEDAKQIQSGVDMIHRQLLDVLNAEQVEVIEAVGQPFDPNFHQAVMQ
EPSDEFESGIVTMELQKGYTMHGRVIRPSMVKVAE
>Mature_194_residues
TADHQNGHEQDDVLEEKSVDSAQTEENESTESNEDEVLNAEIVEESSEGTDLQKEIEALKASELRIRADFDNFRRRTNEE
NAKRVKFASQSVIEKLIPLIDNFERALQVNATSEDAKQIQSGVDMIHRQLLDVLNAEQVEVIEAVGQPFDPNFHQAVMQE
PSDEFESGIVTMELQKGYTMHGRVIRPSMVKVAE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=203, Percent_Identity=28.0788177339901, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1788967, Length=132, Percent_Identity=40.9090909090909, Blast_Score=104, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324806, Length=189, Percent_Identity=33.8624338624339, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24653432, Length=178, Percent_Identity=30.8988764044944, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22072; Mature: 21940

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADHQNGHEQDDVLEEKSVDSAQTEENESTESNEDEVLNAEIVEESSEGTDLQKEIEAL
CCCCCCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
KASELRIRADFDNFRRRTNEENAKRVKFASQSVIEKLIPLIDNFERALQVNATSEDAKQI
HHHHHEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
QSGVDMIHRQLLDVLNAEQVEVIEAVGQPFDPNFHQAVMQEPSDEFESGIVTMELQKGYT
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHCCEEEEEECCCCC
MHGRVIRPSMVKVAE
CCCEEECCHHHHHCC
>Mature Secondary Structure 
TADHQNGHEQDDVLEEKSVDSAQTEENESTESNEDEVLNAEIVEESSEGTDLQKEIEAL
CCCCCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
KASELRIRADFDNFRRRTNEENAKRVKFASQSVIEKLIPLIDNFERALQVNATSEDAKQI
HHHHHEEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
QSGVDMIHRQLLDVLNAEQVEVIEAVGQPFDPNFHQAVMQEPSDEFESGIVTMELQKGYT
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHCCEEEEEECCCCC
MHGRVIRPSMVKVAE
CCCEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA