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The map label for this gene is cysM [H]

Identifier: 229916339

GI number: 229916339

Start: 558772

End: 559710

Strand: Reverse

Name: cysM [H]

Synonym: EAT1b_0609

Alternate gene names: 229916339

Gene position: 559710-558772 (Counterclockwise)

Preceding gene: 229916340

Following gene: 229916338

Centisome position: 18.66

GC content: 50.69

Gene sequence:

>939_bases
ATGATTGTCCGTGACGTCTATGAACTCATCGGAGACACACCGCTCATTGAACTTCGAACGCTTCCTGTACCAGCAAATGT
CAAAGTGTACGGGAAGCTCGAGATGATGAATCCAGGTGGGAGTGTTAAAGACCGACTCGGGATGAATTTGATTGAACGGG
CGATCGAGCGTGGCGATGTGGCACCCGGTGGAACGGTGATCGAACCGACAGCAGGTAATACGGGGATCGGGCTTGCGCTC
GCTTGCCAACGATTCGGGATTCGCCTCATGACGGTCACTCCTGAAAAATTCAGTCAAGAGAAGCAGGCATTGATGCGACT
GCTCGGAGCAACTGTCGTCAACACGCCGACCGAACTCGGCATGAAGGGCGCGATCGCACATGCCATTGAGTTGGCTAAAG
AACATGACGCCTATGTCCCAGAACAGTTCTCGAACCCGGATAACCCGGATGCTTACGTCGACATCCTTTCCCATGAGTTG
TTGAAAGATGTGCCACAAATTGACGTATTTGTAGCGGGAGCGGGGTCAGGTGGCACATTCACCGGTGTCGCCAAATCATT
AAAGCCACTTGGAACGAAAACTGTCGTCGTAGAGCCGGTCGGTTCCATTTTGAATGGCGGTGAACCAGGACCACATAAAA
CAGAAGGAATCGGCGTCGAAAAATGGCCACCCTTTTTGGAGAGGGCTTACGTCGATGCGATTCATACCATTACAGATGAG
GAAGCCTTCTACTACGTGGCACATTTAGCGAAACAAGAAGGTTTGCTCATCGGTAGTTCTTCAGGCGCTGCCATTGCAGC
ATGTGTAAAAGAAGCGAGTCAATTGACGGAAGGCACGATTGTCACCATCTTGCCTGATAGTGCAGAACGATATTTAAGTC
AAGGAATTGTCAAGGAGGCAGAACATGCGTACAAAGACAGCACTCATTCACGGCGGTAA

Upstream 100 bases:

>100_bases
AATGCGAACCATCATGATTTCGAAGGTGCCAAAAAATTAGCGGCAGACATGCTCGCAAAACGCGATGAATGGCATATCGT
CTTCAAGGACGAGGCATAAG

Downstream 100 bases:

>100_bases
AACGACGGACCCGTTGACAGGGGCCGTCACGCCACCGATTTATCAAACGAGCACGTATAAGCAAGACGGGATTGGCAACT
TGCGAGAAGGGTACGAGTAC

Product: Pyridoxal-5'-phosphate-dependent protein subunit beta

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIVRDVYELIGDTPLIELRTLPVPANVKVYGKLEMMNPGGSVKDRLGMNLIERAIERGDVAPGGTVIEPTAGNTGIGLAL
ACQRFGIRLMTVTPEKFSQEKQALMRLLGATVVNTPTELGMKGAIAHAIELAKEHDAYVPEQFSNPDNPDAYVDILSHEL
LKDVPQIDVFVAGAGSGGTFTGVAKSLKPLGTKTVVVEPVGSILNGGEPGPHKTEGIGVEKWPPFLERAYVDAIHTITDE
EAFYYVAHLAKQEGLLIGSSSGAAIAACVKEASQLTEGTIVTILPDSAERYLSQGIVKEAEHAYKDSTHSRR

Sequences:

>Translated_312_residues
MIVRDVYELIGDTPLIELRTLPVPANVKVYGKLEMMNPGGSVKDRLGMNLIERAIERGDVAPGGTVIEPTAGNTGIGLAL
ACQRFGIRLMTVTPEKFSQEKQALMRLLGATVVNTPTELGMKGAIAHAIELAKEHDAYVPEQFSNPDNPDAYVDILSHEL
LKDVPQIDVFVAGAGSGGTFTGVAKSLKPLGTKTVVVEPVGSILNGGEPGPHKTEGIGVEKWPPFLERAYVDAIHTITDE
EAFYYVAHLAKQEGLLIGSSSGAAIAACVKEASQLTEGTIVTILPDSAERYLSQGIVKEAEHAYKDSTHSRR
>Mature_312_residues
MIVRDVYELIGDTPLIELRTLPVPANVKVYGKLEMMNPGGSVKDRLGMNLIERAIERGDVAPGGTVIEPTAGNTGIGLAL
ACQRFGIRLMTVTPEKFSQEKQALMRLLGATVVNTPTELGMKGAIAHAIELAKEHDAYVPEQFSNPDNPDAYVDILSHEL
LKDVPQIDVFVAGAGSGGTFTGVAKSLKPLGTKTVVVEPVGSILNGGEPGPHKTEGIGVEKWPPFLERAYVDAIHTITDE
EAFYYVAHLAKQEGLLIGSSSGAAIAACVKEASQLTEGTIVTILPDSAERYLSQGIVKEAEHAYKDSTHSRR

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=309, Percent_Identity=40.7766990291262, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI295821200, Length=309, Percent_Identity=40.7766990291262, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI4557415, Length=309, Percent_Identity=40.7766990291262, Blast_Score=207, Evalue=7e-54,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=42.4920127795527, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=34.2105263157895, Blast_Score=167, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI25147552, Length=313, Percent_Identity=37.0607028753994, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=36.3333333333333, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17534315, Length=315, Percent_Identity=35.5555555555556, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI32566674, Length=300, Percent_Identity=35.3333333333333, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=34.8837209302326, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI115535073, Length=297, Percent_Identity=39.3939393939394, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17561720, Length=302, Percent_Identity=35.7615894039735, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32566672, Length=207, Percent_Identity=34.2995169082126, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71996324, Length=202, Percent_Identity=26.7326732673267, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=321, Percent_Identity=38.3177570093458, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=333, Percent_Identity=33.6336336336336, Blast_Score=126, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=38.1877022653722, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=38.1877022653722, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33339; Mature: 33339

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVRDVYELIGDTPLIELRTLPVPANVKVYGKLEMMNPGGSVKDRLGMNLIERAIERGDV
CCHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
APGGTVIEPTAGNTGIGLALACQRFGIRLMTVTPEKFSQEKQALMRLLGATVVNTPTELG
CCCCEEEECCCCCCCCHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHC
MKGAIAHAIELAKEHDAYVPEQFSNPDNPDAYVDILSHELLKDVPQIDVFVAGAGSGGTF
HHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
TGVAKSLKPLGTKTVVVEPVGSILNGGEPGPHKTEGIGVEKWPPFLERAYVDAIHTITDE
HHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
EAFYYVAHLAKQEGLLIGSSSGAAIAACVKEASQLTEGTIVTILPDSAERYLSQGIVKEA
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
EHAYKDSTHSRR
HHHHHCCCCCCC
>Mature Secondary Structure
MIVRDVYELIGDTPLIELRTLPVPANVKVYGKLEMMNPGGSVKDRLGMNLIERAIERGDV
CCHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
APGGTVIEPTAGNTGIGLALACQRFGIRLMTVTPEKFSQEKQALMRLLGATVVNTPTELG
CCCCEEEECCCCCCCCHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHC
MKGAIAHAIELAKEHDAYVPEQFSNPDNPDAYVDILSHELLKDVPQIDVFVAGAGSGGTF
HHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
TGVAKSLKPLGTKTVVVEPVGSILNGGEPGPHKTEGIGVEKWPPFLERAYVDAIHTITDE
HHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
EAFYYVAHLAKQEGLLIGSSSGAAIAACVKEASQLTEGTIVTILPDSAERYLSQGIVKEA
HHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
EHAYKDSTHSRR
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]