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The map label for this gene is ispH

Identifier: 229916316

GI number: 229916316

Start: 536226

End: 537179

Strand: Direct

Name: ispH

Synonym: EAT1b_0585

Alternate gene names: 229916316

Gene position: 536226-537179 (Clockwise)

Preceding gene: 229916310

Following gene: 229916342

Centisome position: 17.87

GC content: 47.59

Gene sequence:

>954_bases
ATGAAAGTTAAAAAAATTAGCCCACGCGGATATTGTTATGGTGTCGTCGATGCCATGAAGCTCGCAAATGAGGCGGTCGC
AAATCCTGATTTGCCACGCCCGATTCATATACTTGGAATGATTGTCCACAACCGTCACGTCACACAGGCGTTTGAGGATC
TCGGTGTCAAAACCGTTGATGGAGAAGACCGCATGCAAGCGCTCGAAACCATCGATCGAGGAACAGTCGTCTTCACGGCG
CACGGCATTTCACCACTCGTCCGGAAACGTGCCATCGAGAAAGGATTGACGATTGTAGATGCCTCTTGTCCTGACGTCTT
GGTCACTCACGATTTGATTCGTGAGAAAACGGCAGAAGGATATGATGTCATCTATATCGGAAAACACGGGCATCCGGAAC
CGGAGGGTGCAATCGGGGTTGCACCAGACCATGTTCATTTGATTCAATATGAACATGAAATCGATCAGTTGCCGAGTCGC
TTGTTCGAACGAAAAATTTTGGTGACGAACCAGACGACGATGAGTCAATGGGACGTTTCCGCATTAATTGAAACGATTCA
AGCCCGTTATCCTCACGTGGAAGTGCATAATGAAATCTGTAACGCGACGCAAGTTCGTCAAGAAGCCGTCGCTGAACAAG
CGGGAGACTGCGAATTGTTGATTGTCGTCGGAGATCCCAAATCAAATAACTCCAATCGTTTAGCACAAGTTTCAAAAGAG
ATTGCCGGAACGAATGCATATCGGATCGGTGACTTGAGTGAACTTGACTTGAATTGGCTTGAAGGCGTGGAGACCGTAGC
AGTGACGTCGGGCGCCTCTACCCCGACACCGATCACGAAAAAAGTCATCGACTTTTTATCGCAATATGACCCAGACGATT
TAAGCACGCACGATAAAACGCCATTTCTTGAACTTCGGAGTATCTTACCGAAAGTGAAAGCATTGCGTAAGTGA

Upstream 100 bases:

>100_bases
TAAAAATTATAGCATGAATACACGCAAATCTTTTCGTATTTTCGAAACAATGTTAGACTAATACGTATGAAAGCACTTCG
AAAGGAGCCTTCGTCTTCAT

Downstream 100 bases:

>100_bases
AGGAATCCCCCATTCTCTCAGAGGATGGGGGATTTTTAGGCTTTACTTGAATTGAAACGGATTCGTGTTGGCAACAGATT
GAATCAAGTCGACATTGATT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKVKKISPRGYCYGVVDAMKLANEAVANPDLPRPIHILGMIVHNRHVTQAFEDLGVKTVDGEDRMQALETIDRGTVVFTA
HGISPLVRKRAIEKGLTIVDASCPDVLVTHDLIREKTAEGYDVIYIGKHGHPEPEGAIGVAPDHVHLIQYEHEIDQLPSR
LFERKILVTNQTTMSQWDVSALIETIQARYPHVEVHNEICNATQVRQEAVAEQAGDCELLIVVGDPKSNNSNRLAQVSKE
IAGTNAYRIGDLSELDLNWLEGVETVAVTSGASTPTPITKKVIDFLSQYDPDDLSTHDKTPFLELRSILPKVKALRK

Sequences:

>Translated_317_residues
MKVKKISPRGYCYGVVDAMKLANEAVANPDLPRPIHILGMIVHNRHVTQAFEDLGVKTVDGEDRMQALETIDRGTVVFTA
HGISPLVRKRAIEKGLTIVDASCPDVLVTHDLIREKTAEGYDVIYIGKHGHPEPEGAIGVAPDHVHLIQYEHEIDQLPSR
LFERKILVTNQTTMSQWDVSALIETIQARYPHVEVHNEICNATQVRQEAVAEQAGDCELLIVVGDPKSNNSNRLAQVSKE
IAGTNAYRIGDLSELDLNWLEGVETVAVTSGASTPTPITKKVIDFLSQYDPDDLSTHDKTPFLELRSILPKVKALRK
>Mature_317_residues
MKVKKISPRGYCYGVVDAMKLANEAVANPDLPRPIHILGMIVHNRHVTQAFEDLGVKTVDGEDRMQALETIDRGTVVFTA
HGISPLVRKRAIEKGLTIVDASCPDVLVTHDLIREKTAEGYDVIYIGKHGHPEPEGAIGVAPDHVHLIQYEHEIDQLPSR
LFERKILVTNQTTMSQWDVSALIETIQARYPHVEVHNEICNATQVRQEAVAEQAGDCELLIVVGDPKSNNSNRLAQVSKE
IAGTNAYRIGDLSELDLNWLEGVETVAVTSGASTPTPITKKVIDFLSQYDPDDLSTHDKTPFLELRSILPKVKALRK

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=293, Percent_Identity=33.4470989761092, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_EXISA (C4L3Z5)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884962.1
- GeneID:   7871324
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0585
- OMA:   WGAPVYV
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 35098; Mature: 35098

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKKISPRGYCYGVVDAMKLANEAVANPDLPRPIHILGMIVHNRHVTQAFEDLGVKTVD
CCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCEEEC
GEDRMQALETIDRGTVVFTAHGISPLVRKRAIEKGLTIVDASCPDVLVTHDLIREKTAEG
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCC
YDVIYIGKHGHPEPEGAIGVAPDHVHLIQYEHEIDQLPSRLFERKILVTNQTTMSQWDVS
CEEEEECCCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHCEEEEECCCHHHHHHHH
ALIETIQARYPHVEVHNEICNATQVRQEAVAEQAGDCELLIVVGDPKSNNSNRLAQVSKE
HHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
IAGTNAYRIGDLSELDLNWLEGVETVAVTSGASTPTPITKKVIDFLSQYDPDDLSTHDKT
HHCCCCEECCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PFLELRSILPKVKALRK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVKKISPRGYCYGVVDAMKLANEAVANPDLPRPIHILGMIVHNRHVTQAFEDLGVKTVD
CCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCEEEC
GEDRMQALETIDRGTVVFTAHGISPLVRKRAIEKGLTIVDASCPDVLVTHDLIREKTAEG
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCC
YDVIYIGKHGHPEPEGAIGVAPDHVHLIQYEHEIDQLPSRLFERKILVTNQTTMSQWDVS
CEEEEECCCCCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHCEEEEECCCHHHHHHHH
ALIETIQARYPHVEVHNEICNATQVRQEAVAEQAGDCELLIVVGDPKSNNSNRLAQVSKE
HHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
IAGTNAYRIGDLSELDLNWLEGVETVAVTSGASTPTPITKKVIDFLSQYDPDDLSTHDKT
HHCCCCEECCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PFLELRSILPKVKALRK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA