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The map label for this gene is amyA [C]

Identifier: 229916298

GI number: 229916298

Start: 514939

End: 516411

Strand: Reverse

Name: amyA [C]

Synonym: EAT1b_0567

Alternate gene names: 229916298

Gene position: 516411-514939 (Counterclockwise)

Preceding gene: 229916299

Following gene: 229916297

Centisome position: 17.21

GC content: 44.81

Gene sequence:

>1473_bases
ATGGAACGAAACCACACCATGATGCAATTTTTTGAATGGCATGTCGAGGCAGACCAAAAGCATTGGAAGCGACTAAAAGA
GAGAGCAGCCGACTTAAATAAAGCAGGGATCACTGCGCTATGGCTACCACCTGTCACAAAAGGGCAAAGTGATCAAGATA
CCGGATATGGAATCTATGACTTATATGATTTGGGCGAGTTCGATCAAAAAGGGTCGATTGCCACAAAATATGGAACGAAA
AAAGAACTACTTGAAGCAACGACCTATTGTCAACAAGAGCTTGGGATGCAGATTTATATGGATGTCGTCATGAATCATAA
AGCCGGGGCGGATGCGACCGAGAAGTTTGACGTGTTAGAAGTCGATCCCGACGACCGTACGAAAGTCCTCTCGGATGAAG
CGTTTGAAATCGAAGGGTGGACAAAATTTGATTATGAAGCTCGTGATGGGAAATACTCTTCATTTAAATGGGACTTCACA
CATTTTAACGGAACGGATTATGATGCGGCGACTGATCGTTCCGGTATTTTCTTTATCGTTGGAGAAAACAAAAGATGGAA
CGATAACGTCGACGATGAATTCGGTGTCTATGACTATTTAATGTTTGCGAACATCGACTACCACCATCCCGATGTGCGCG
AAGAAGTGTTGAATTGGGGGAAATGGTTCATCGAAGAGACGAAATGTGATGGATTCCGTCTTGACGCCATTAAACACATC
AACTATGACTTTGTAAAAGAGTTCAGTCAGACGATGCGGGAAGAGAAAGGTGACCAATTCTATTTTGTCGGCGAGTTTTG
GAAATCTGACCTTGATGCCTGTCGTGAATTTCTCGATAACATCGATTACCATGTCGATTTATTCGATGTCCCGCTCCACT
ACAAATTACATCAAGCCTCAGAGGAAGGGGCCGCGTTTGACATGCGAACCATTTTTGATGATACGCTCGTTCAGTCACAC
CCGCTTCATTCCGTGACGTTCGTAGATAATCACGATTCACAACCTGAAGAGGCGCTTGAGTCATGGGTGAAAGATTGGTT
CAAGCAAATCGCCTATGGTCTCGTCTTACTTCGTAAAGATGGTTATCCATGTGTCTTCTATGGCGATTACTACGGTATAG
ACGGTCCTCACCCAACGGATGGGATGAAGCTCGCCATTGATACGCTTCTGTATGCGCGCCGAGAGAAAGCGTATGGGGAG
CAGGATGATTACTTTGATGATGCAAACGTCATTGGGTGGGTACGAAAAGGTGTGGAGGAAATCGAACGTTCCGGATGTGC
CGTTCTCATTTCAACTTCTGATGAAGCTGAAAAGAGAATGTTTGTCGGGGAAGAACGTGCCGGTGAAACTTGGGTCGATT
TAACGAATACGTTGGATGATCGAATTGAGATTGATGAAGAAGGATTTGGGGTATTTAAAGTGTCGGCGGGGTCGATTTCA
GTCTGGGCATTGCCAGAAGAAGATGTCGAATAA

Upstream 100 bases:

>100_bases
TCACATTGTGAACGTCGCTTTTTTGTTTTCAATCTAAAATTGCTGGGTAAATACTCGTTAGCATTTTGTTTTTTGAAAAA
TATGAAATGAGGCGGACAAA

Downstream 100 bases:

>100_bases
AGCATTACAATTGAGTCGACCGTCACATATCTGTTACATTTAAGACGAACGTTGTTCAAAACACTTTTAAGGGGGATTTT
AGTAATGGCTAAATTTGAAT

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: Exo-maltohexaohydrolase; G6-amylase; Maltohexaose-producing amylase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MERNHTMMQFFEWHVEADQKHWKRLKERAADLNKAGITALWLPPVTKGQSDQDTGYGIYDLYDLGEFDQKGSIATKYGTK
KELLEATTYCQQELGMQIYMDVVMNHKAGADATEKFDVLEVDPDDRTKVLSDEAFEIEGWTKFDYEARDGKYSSFKWDFT
HFNGTDYDAATDRSGIFFIVGENKRWNDNVDDEFGVYDYLMFANIDYHHPDVREEVLNWGKWFIEETKCDGFRLDAIKHI
NYDFVKEFSQTMREEKGDQFYFVGEFWKSDLDACREFLDNIDYHVDLFDVPLHYKLHQASEEGAAFDMRTIFDDTLVQSH
PLHSVTFVDNHDSQPEEALESWVKDWFKQIAYGLVLLRKDGYPCVFYGDYYGIDGPHPTDGMKLAIDTLLYARREKAYGE
QDDYFDDANVIGWVRKGVEEIERSGCAVLISTSDEAEKRMFVGEERAGETWVDLTNTLDDRIEIDEEGFGVFKVSAGSIS
VWALPEEDVE

Sequences:

>Translated_490_residues
MERNHTMMQFFEWHVEADQKHWKRLKERAADLNKAGITALWLPPVTKGQSDQDTGYGIYDLYDLGEFDQKGSIATKYGTK
KELLEATTYCQQELGMQIYMDVVMNHKAGADATEKFDVLEVDPDDRTKVLSDEAFEIEGWTKFDYEARDGKYSSFKWDFT
HFNGTDYDAATDRSGIFFIVGENKRWNDNVDDEFGVYDYLMFANIDYHHPDVREEVLNWGKWFIEETKCDGFRLDAIKHI
NYDFVKEFSQTMREEKGDQFYFVGEFWKSDLDACREFLDNIDYHVDLFDVPLHYKLHQASEEGAAFDMRTIFDDTLVQSH
PLHSVTFVDNHDSQPEEALESWVKDWFKQIAYGLVLLRKDGYPCVFYGDYYGIDGPHPTDGMKLAIDTLLYARREKAYGE
QDDYFDDANVIGWVRKGVEEIERSGCAVLISTSDEAEKRMFVGEERAGETWVDLTNTLDDRIEIDEEGFGVFKVSAGSIS
VWALPEEDVE
>Mature_490_residues
MERNHTMMQFFEWHVEADQKHWKRLKERAADLNKAGITALWLPPVTKGQSDQDTGYGIYDLYDLGEFDQKGSIATKYGTK
KELLEATTYCQQELGMQIYMDVVMNHKAGADATEKFDVLEVDPDDRTKVLSDEAFEIEGWTKFDYEARDGKYSSFKWDFT
HFNGTDYDAATDRSGIFFIVGENKRWNDNVDDEFGVYDYLMFANIDYHHPDVREEVLNWGKWFIEETKCDGFRLDAIKHI
NYDFVKEFSQTMREEKGDQFYFVGEFWKSDLDACREFLDNIDYHVDLFDVPLHYKLHQASEEGAAFDMRTIFDDTLVQSH
PLHSVTFVDNHDSQPEEALESWVKDWFKQIAYGLVLLRKDGYPCVFYGDYYGIDGPHPTDGMKLAIDTLLYARREKAYGE
QDDYFDDANVIGWVRKGVEEIERSGCAVLISTSDEAEKRMFVGEERAGETWVDLTNTLDDRIEIDEEGFGVFKVSAGSIS
VWALPEEDVE

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=496, Percent_Identity=43.75, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR006046
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.98 [H]

Molecular weight: Translated: 56969; Mature: 56969

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNHTMMQFFEWHVEADQKHWKRLKERAADLNKAGITALWLPPVTKGQSDQDTGYGIYD
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
LYDLGEFDQKGSIATKYGTKKELLEATTYCQQELGMQIYMDVVMNHKAGADATEKFDVLE
ECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCHHEEEHHHHCCCCCCCCCCCCEEEE
VDPDDRTKVLSDEAFEIEGWTKFDYEARDGKYSSFKWDFTHFNGTDYDAATDRSGIFFIV
ECCCCCCEECCCCCEEECCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
GENKRWNDNVDDEFGVYDYLMFANIDYHHPDVREEVLNWGKWFIEETKCDGFRLDAIKHI
ECCCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHC
NYDFVKEFSQTMREEKGDQFYFVGEFWKSDLDACREFLDNIDYHVDLFDVPLHYKLHQAS
CHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCC
EEGAAFDMRTIFDDTLVQSHPLHSVTFVDNHDSQPEEALESWVKDWFKQIAYGLVLLRKD
CCCCCEEHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECC
GYPCVFYGDYYGIDGPHPTDGMKLAIDTLLYARREKAYGEQDDYFDDANVIGWVRKGVEE
CCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
IERSGCAVLISTSDEAEKRMFVGEERAGETWVDLTNTLDDRIEIDEEGFGVFKVSAGSIS
HHHCCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCEE
VWALPEEDVE
EEECCHHCCC
>Mature Secondary Structure
MERNHTMMQFFEWHVEADQKHWKRLKERAADLNKAGITALWLPPVTKGQSDQDTGYGIYD
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
LYDLGEFDQKGSIATKYGTKKELLEATTYCQQELGMQIYMDVVMNHKAGADATEKFDVLE
ECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCHHEEEHHHHCCCCCCCCCCCCEEEE
VDPDDRTKVLSDEAFEIEGWTKFDYEARDGKYSSFKWDFTHFNGTDYDAATDRSGIFFIV
ECCCCCCEECCCCCEEECCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
GENKRWNDNVDDEFGVYDYLMFANIDYHHPDVREEVLNWGKWFIEETKCDGFRLDAIKHI
ECCCCCCCCCCCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHC
NYDFVKEFSQTMREEKGDQFYFVGEFWKSDLDACREFLDNIDYHVDLFDVPLHYKLHQAS
CHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCC
EEGAAFDMRTIFDDTLVQSHPLHSVTFVDNHDSQPEEALESWVKDWFKQIAYGLVLLRKD
CCCCCEEHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEECC
GYPCVFYGDYYGIDGPHPTDGMKLAIDTLLYARREKAYGEQDDYFDDANVIGWVRKGVEE
CCCEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
IERSGCAVLISTSDEAEKRMFVGEERAGETWVDLTNTLDDRIEIDEEGFGVFKVSAGSIS
HHHCCCEEEEECCCCHHHHHCCCCCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCEE
VWALPEEDVE
EEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3258152 [H]