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The map label for this gene is yhfE [H]

Identifier: 229916283

GI number: 229916283

Start: 499415

End: 500437

Strand: Reverse

Name: yhfE [H]

Synonym: EAT1b_0552

Alternate gene names: 229916283

Gene position: 500437-499415 (Counterclockwise)

Preceding gene: 229916284

Following gene: 229916282

Centisome position: 16.68

GC content: 49.95

Gene sequence:

>1023_bases
ATGATTGAAACATTAAAAACACTCGTTAACATCCCGAGTCCTTCCGGGATGACGGAAGAGGCCATCCGCTTCGCGGAGCA
AGAATTGAATGCATTGGGTGTCAAGACGTCACGTCTACATAAAGGGGCACTGCTGGCCACATTTTCAGGTCGTTCTGACA
AGGCGCGTCTGTTGACGGCACACGTGGACACACTCGGTGCGATGGTCAAAGACATCTTGCCGAGCGGGCGTCTTCGTTTG
ACTCAAGTGGGAGGATACGCATGGACGGCGATTGAGGGAGAGAACTGCCTCGTCCACCGTGAAGAAGGAGAGGCGATTGA
AGGGACGATCTTGATCCATCAGTCGAGTGTTCACGTCTATAAGGACACGAACTCTGCCGAGCGATCTGCATCAAACATCG
AAGTCCGATTGGATGAAAAAGTGCATTCTCGTGAAGATGTGACGGCGCTTGGGATTGAAATCGGGGACTTCGTTTCATTC
GATCCACGCTTTCGAGTGACCTCTTCAGGATACGTGAAATCACGCCACTTGGATGATAAGGCGTCGGTCGCCATTCTATT
GGACCTTGCGAAAGACTTAATCCGTTATGACTTACCGCATACCGTTCACTTCTTGATTTCGAATTATGAAGAGGTCGGAT
TTGGAGGGAACGCGGGTATTCCGGAAGACGTCGTGGAATACATTGCCGTTGATATGGGCGCGCTCGGGGATGGACAGCAT
TCCGATGAGTACACAGTCTCGATTTGTGCCAAGGATTCGTCTGGACCGTATGATATCGGGTTACGACGGAAGTTCACGGC
GTTGTGTAAAGCTCATGAGATCCCGTATAAGGTAGATATTTATCCGTATTATGGGTCAGATGCGTCGGCGGCGATTCGAG
CAGGGTATGATGTGCGACATGCCTTGATTGGGCCAGGAATCGAATCGAGCCATAGTTATGAGCGAACGCATGTGTCATCA
ATGGAAGCAACGAGAGAATTGTTAAATCGTTATGTACAATCGACGATGGAGGAAGAAGCATGA

Upstream 100 bases:

>100_bases
GGATGAGAAGAACCGTATACAACGAATCTTATTTTCCGTACTCACCATCTCGATGTTTGGACTATTCGTCGGGTACGGAT
TTTGGTAAGGGGAGAATTCA

Downstream 100 bases:

>100_bases
AAAACTTTTATGATGTCCAACAGTTTTTAAAATCGTTCGGTACCATTATTTACATCGGAGAACGGGATGCTGAAATCTCA
CTCATGATGATGGAACTCGA

Product: peptidase M42 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MIETLKTLVNIPSPSGMTEEAIRFAEQELNALGVKTSRLHKGALLATFSGRSDKARLLTAHVDTLGAMVKDILPSGRLRL
TQVGGYAWTAIEGENCLVHREEGEAIEGTILIHQSSVHVYKDTNSAERSASNIEVRLDEKVHSREDVTALGIEIGDFVSF
DPRFRVTSSGYVKSRHLDDKASVAILLDLAKDLIRYDLPHTVHFLISNYEEVGFGGNAGIPEDVVEYIAVDMGALGDGQH
SDEYTVSICAKDSSGPYDIGLRRKFTALCKAHEIPYKVDIYPYYGSDASAAIRAGYDVRHALIGPGIESSHSYERTHVSS
MEATRELLNRYVQSTMEEEA

Sequences:

>Translated_340_residues
MIETLKTLVNIPSPSGMTEEAIRFAEQELNALGVKTSRLHKGALLATFSGRSDKARLLTAHVDTLGAMVKDILPSGRLRL
TQVGGYAWTAIEGENCLVHREEGEAIEGTILIHQSSVHVYKDTNSAERSASNIEVRLDEKVHSREDVTALGIEIGDFVSF
DPRFRVTSSGYVKSRHLDDKASVAILLDLAKDLIRYDLPHTVHFLISNYEEVGFGGNAGIPEDVVEYIAVDMGALGDGQH
SDEYTVSICAKDSSGPYDIGLRRKFTALCKAHEIPYKVDIYPYYGSDASAAIRAGYDVRHALIGPGIESSHSYERTHVSS
MEATRELLNRYVQSTMEEEA
>Mature_340_residues
MIETLKTLVNIPSPSGMTEEAIRFAEQELNALGVKTSRLHKGALLATFSGRSDKARLLTAHVDTLGAMVKDILPSGRLRL
TQVGGYAWTAIEGENCLVHREEGEAIEGTILIHQSSVHVYKDTNSAERSASNIEVRLDEKVHSREDVTALGIEIGDFVSF
DPRFRVTSSGYVKSRHLDDKASVAILLDLAKDLIRYDLPHTVHFLISNYEEVGFGGNAGIPEDVVEYIAVDMGALGDGQH
SDEYTVSICAKDSSGPYDIGLRRKFTALCKAHEIPYKVDIYPYYGSDASAAIRAGYDVRHALIGPGIESSHSYERTHVSS
MEATRELLNRYVQSTMEEEA

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1788727, Length=317, Percent_Identity=24.2902208201893, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: NA

Molecular weight: Translated: 37403; Mature: 37403

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETLKTLVNIPSPSGMTEEAIRFAEQELNALGVKTSRLHKGALLATFSGRSDKARLLTA
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCEEEEH
HVDTLGAMVKDILPSGRLRLTQVGGYAWTAIEGENCLVHREEGEAIEGTILIHQSSVHVY
HHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCEEEECCCCCEEEEEEEEEECCEEEE
KDTNSAERSASNIEVRLDEKVHSREDVTALGIEIGDFVSFDPRFRVTSSGYVKSRHLDDK
ECCCCHHCCCCCEEEEECCHHCCCCCEEEEEEEECCCCCCCCCEEEECCCCEEECCCCCH
ASVAILLDLAKDLIRYDLPHTVHFLISNYEEVGFGGNAGIPEDVVEYIAVDMGALGDGQH
HHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
SDEYTVSICAKDSSGPYDIGLRRKFTALCKAHEIPYKVDIYPYYGSDASAAIRAGYDVRH
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHCCCCHHH
ALIGPGIESSHSYERTHVSSMEATRELLNRYVQSTMEEEA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIETLKTLVNIPSPSGMTEEAIRFAEQELNALGVKTSRLHKGALLATFSGRSDKARLLTA
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCEEEEH
HVDTLGAMVKDILPSGRLRLTQVGGYAWTAIEGENCLVHREEGEAIEGTILIHQSSVHVY
HHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCEEEECCCCCEEEEEEEEEECCEEEE
KDTNSAERSASNIEVRLDEKVHSREDVTALGIEIGDFVSFDPRFRVTSSGYVKSRHLDDK
ECCCCHHCCCCCEEEEECCHHCCCCCEEEEEEEECCCCCCCCCEEEECCCCEEECCCCCH
ASVAILLDLAKDLIRYDLPHTVHFLISNYEEVGFGGNAGIPEDVVEYIAVDMGALGDGQH
HHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
SDEYTVSICAKDSSGPYDIGLRRKFTALCKAHEIPYKVDIYPYYGSDASAAIRAGYDVRH
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHCCCCHHH
ALIGPGIESSHSYERTHVSSMEATRELLNRYVQSTMEEEA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]