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The map label for this gene is glcK [H]

Identifier: 229916281

GI number: 229916281

Start: 498168

End: 499130

Strand: Reverse

Name: glcK [H]

Synonym: EAT1b_0550

Alternate gene names: 229916281

Gene position: 499130-498168 (Counterclockwise)

Preceding gene: 229916282

Following gene: 229916280

Centisome position: 16.64

GC content: 49.01

Gene sequence:

>963_bases
ATGAAATGGTTATTAGGTATCGACATCGGTGGTACGACGGTGAAGATGGCCGTTTTAGACATGAACGGCATCATTTCGGA
TAAGTGGGAAGTCAAAACGGATATTCGAGAGAATGGTGTACATATTCCAAAAGACATCGCAGCTTCTTTTGAAACATACT
TACAAACATCAGGTAAGAAGAAAGAAGATTTTGCGGGTGCGGGAATCGGGGCACCAGGATTCGTCAACTTCTCAGAAGGA
GTTGTCGAATATTCTCCGAACATCGGGTGGAAAGATTTTGCTCTCGTGAGTGAGTTCGAAAAAGCAGTCGGTCTTCCGGC
CGTGCTCGAAAATGATGCGAACGCCGCGGCCCTTGGTGAGATGTGGAAAGGTGCCGGCGAAGGGGCATCAGAATTATTGG
CGATCACGCTCGGTACAGGTGTCGGTGGTGGCGTGATCACGAACGGAAATATCGTTCATGGGACGGCAGGAATGGCCGGA
GAAATCGGGCATATTACCGTGGAACGAGACGAAACGAAAGCTGTGAAGTGTGGTTGTGGTCGCCTCGGGTGTATTGAGAC
GATTGCTTCAGCGACCGGCATCTCGCGTTTGGCGTTACAGAAGCGTAAAAATCAAGATACTTCATTAAACGAGTTAAAAG
AAGTGACTGCACGTGACGTGTTTGAAGCATATAAGGCTGGTGACTCGATCGCGACAGAAGTCATTGAAGAGATGACGGAA
TACCTCGGCTTGACCATCTCAAACATCGCGAACACGCTCAATCCGAAAATGATTGTCATCGGTGGAGGTGTCTCAAAAGC
TGGTGACGCGTTATTGGAGCCGCTTGATGCACAGTTCAAACGGTTTGCGCTCGAGCGGGTATACGAGTCGACAACATTCA
AAATCGCTGAACTCGAAAACGATGCCGGTGTGATTGGATGTGCTTGGCTGGCACGGAAACACTTCCTTCCGACACGAGTA
TGA

Upstream 100 bases:

>100_bases
AGATTGACAGAAATCCCCTTAGTGCAAAGGGTTGCACTAAGGGGATAATAGTGTACACTATTCAATGAAAGCGATTTAGT
TGAGATGAGGAGTGAACGGT

Downstream 100 bases:

>100_bases
AACGGACGCGTCTGCACATTGCAGGCGTGTTTTTTTGTGGGAAAGGGTATGGGATATTGTAAAATTTGTAACGTCACAGT
TGATTTTTTATTAAAAAAGG

Product: glucokinase, ROK family

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKWLLGIDIGGTTVKMAVLDMNGIISDKWEVKTDIRENGVHIPKDIAASFETYLQTSGKKKEDFAGAGIGAPGFVNFSEG
VVEYSPNIGWKDFALVSEFEKAVGLPAVLENDANAAALGEMWKGAGEGASELLAITLGTGVGGGVITNGNIVHGTAGMAG
EIGHITVERDETKAVKCGCGRLGCIETIASATGISRLALQKRKNQDTSLNELKEVTARDVFEAYKAGDSIATEVIEEMTE
YLGLTISNIANTLNPKMIVIGGGVSKAGDALLEPLDAQFKRFALERVYESTTFKIAELENDAGVIGCAWLARKHFLPTRV

Sequences:

>Translated_320_residues
MKWLLGIDIGGTTVKMAVLDMNGIISDKWEVKTDIRENGVHIPKDIAASFETYLQTSGKKKEDFAGAGIGAPGFVNFSEG
VVEYSPNIGWKDFALVSEFEKAVGLPAVLENDANAAALGEMWKGAGEGASELLAITLGTGVGGGVITNGNIVHGTAGMAG
EIGHITVERDETKAVKCGCGRLGCIETIASATGISRLALQKRKNQDTSLNELKEVTARDVFEAYKAGDSIATEVIEEMTE
YLGLTISNIANTLNPKMIVIGGGVSKAGDALLEPLDAQFKRFALERVYESTTFKIAELENDAGVIGCAWLARKHFLPTRV
>Mature_320_residues
MKWLLGIDIGGTTVKMAVLDMNGIISDKWEVKTDIRENGVHIPKDIAASFETYLQTSGKKKEDFAGAGIGAPGFVNFSEG
VVEYSPNIGWKDFALVSEFEKAVGLPAVLENDANAAALGEMWKGAGEGASELLAITLGTGVGGGVITNGNIVHGTAGMAG
EIGHITVERDETKAVKCGCGRLGCIETIASATGISRLALQKRKNQDTSLNELKEVTARDVFEAYKAGDSIATEVIEEMTE
YLGLTISNIANTLNPKMIVIGGGVSKAGDALLEPLDAQFKRFALERVYESTTFKIAELENDAGVIGCAWLARKHFLPTRV

Specific function: Acts As A Repressor Of The Nagebacd Operon And Acts Both As An Activator And A Repressor For The Transcription Of The Glmsu Operon. [C]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=273, Percent_Identity=30.4029304029304, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI298566315, Length=273, Percent_Identity=30.4029304029304, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI4885285, Length=273, Percent_Identity=30.4029304029304, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI190014632, Length=273, Percent_Identity=30.4029304029304, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1786891, Length=260, Percent_Identity=30, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787878, Length=267, Percent_Identity=28.4644194756554, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787363, Length=273, Percent_Identity=28.9377289377289, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081733, Length=317, Percent_Identity=29.9684542586751, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI87082230, Length=264, Percent_Identity=30.6818181818182, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87082127, Length=285, Percent_Identity=24.9122807017544, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1790522, Length=258, Percent_Identity=26.7441860465116, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 33984; Mature: 33984

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWLLGIDIGGTTVKMAVLDMNGIISDKWEVKTDIRENGVHIPKDIAASFETYLQTSGKK
CCEEEEEECCCCEEEEEEEECCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHCCCCC
KEDFAGAGIGAPGFVNFSEGVVEYSPNIGWKDFALVSEFEKAVGLPAVLENDANAAALGE
CHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHH
MWKGAGEGASELLAITLGTGVGGGVITNGNIVHGTAGMAGEIGHITVERDETKAVKCGCG
HHCCCCCCHHHEEEEEECCCCCCCEEECCCEEECCCCCCCCCEEEEEECCCCCCEECCCC
RLGCIETIASATGISRLALQKRKNQDTSLNELKEVTARDVFEAYKAGDSIATEVIEEMTE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YLGLTISNIANTLNPKMIVIGGGVSKAGDALLEPLDAQFKRFALERVYESTTFKIAELEN
HHCEEHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DAGVIGCAWLARKHFLPTRV
CCCEEHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKWLLGIDIGGTTVKMAVLDMNGIISDKWEVKTDIRENGVHIPKDIAASFETYLQTSGKK
CCEEEEEECCCCEEEEEEEECCCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHCCCCC
KEDFAGAGIGAPGFVNFSEGVVEYSPNIGWKDFALVSEFEKAVGLPAVLENDANAAALGE
CHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHH
MWKGAGEGASELLAITLGTGVGGGVITNGNIVHGTAGMAGEIGHITVERDETKAVKCGCG
HHCCCCCCHHHEEEEEECCCCCCCEEECCCEEECCCCCCCCCEEEEEECCCCCCEECCCC
RLGCIETIASATGISRLALQKRKNQDTSLNELKEVTARDVFEAYKAGDSIATEVIEEMTE
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YLGLTISNIANTLNPKMIVIGGGVSKAGDALLEPLDAQFKRFALERVYESTTFKIAELEN
HHCEEHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DAGVIGCAWLARKHFLPTRV
CCCEEHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]