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The map label for this gene is metG1 [H]

Identifier: 229916147

GI number: 229916147

Start: 379554

End: 381197

Strand: Reverse

Name: metG1 [H]

Synonym: EAT1b_0416

Alternate gene names: 229916147

Gene position: 381197-379554 (Counterclockwise)

Preceding gene: 229916148

Following gene: 229916145

Centisome position: 12.71

GC content: 38.87

Gene sequence:

>1644_bases
ATGAGTATATTTATTGGTGGTGCTTGGGTTTATGCAAATGGTTCATTGCATCTAGGGCATATTTCTAGCCTACTTTCAGG
TGATATCTTAGCAAGATATTATCGGAGTAAAGGGGAGGACGTTCTATATGTTTCAGGAAGTGATTGTAATGGAACACCAA
TCACTGTTCGTGCTAAACAAGAAGGTGTATCTGTTACGGAAATTGCTGATCGATACCATGATGAGTTCGTAGCGTGTTAT
TCATCCTTAGGTTTCTCTTATGATGTGTATGCACGTACCGATCATTCCCAGCATCATGAAGTGGTACAAGACATATTTGC
TGAACTCTACTCAAAAGGTTTCATTTATAAAAAAGAAGTGGAACAAGCCTATTGTGAAACATGTGATCAATTTTTACCGG
ATCGCTATGTTGAAGGTACTTGTCCAGTTTGTCATGGTCCCTCTCGTGGCGATCAATGTGATCACTGCTCAACAATTTTG
GAACCATTAGATTTATTAGAACCTCGATGTAAGCATTGCTTAAACGAACCGTCCACACGCTCTACCGAACATTTTTACTT
TTCATTGAGTTCGTTTCAAAAATTTCTTGAGAAATATACAGAAAATGCGGACCGTCAATCGTTATGGAGGGAAAACGCGA
TTTCTTTGACGAAGCGTTACTTACAAGAAGGTCTTCACGACCGTGCGGTATCACGTGATTTATCTACTGGTGTGAATATT
CCAATTCTAGGATATGAAGAGAAAAAGGTGTATGTGTGGATTGAGGCGATTTCAGGATATTACTCTGCAAGCCGATTGTG
GGCGCAATCAGTAGGAGCTGATGAGGCTACTTTCTGGTCCAATACAACTCGAGCTTATTACGTTCATGGGAAAGATAATA
TCCCTTTCCATTCTATCATCTGGCCAGCGATTTTAGAGGCTCTTGGAGACAAAGCACTCCCGACTCATATCGTATCTAAC
GAATATCTGACGCTAGAAAAAAGAAAGCTCTCAACAAGTAGGAATTGGGCAGTGTGGCTTCCTGATATGTTAGAACGATA
TGAACCAGATTCTATTCGCTATTTCTTAACGATTAATGCACCTGAAAATCGGGATGCAGATTTCTCATGGAAGGAGTTTA
TTTATAGCCACAATGCAGAACTCTTAGGGGCCTATGGGAACTTTGTAAATCGAACATTAAAATTTATCGAGAAATCTTTT
GATCATTCTATACCGAACGACAATATTTCAAGTGAGATTGAAGAACGGATAAGCAATTTGTACGCAGAAGTAGGTCAGTT
AATCGAAGATACGAATTTTAAACAAGCATTGGAATTGATTTTTGAAGCGATTCGATTTTCGAACAAATATTTCGATCAAA
CACAACCATGGAATACGATTAAGGAAAATCCTGCGGAATGCAGACAAACATTAGCGGACTGTACGTTTTTAATCGTGAAT
TTTGGTAATGTATTGAGGCCATTCTTACCATTTTCGAGTCAAAAAATACGTGATGCAATAGAGGTGGACAAGGAAGGGTG
GGAGGTATTGAAAGTGAAATCTTCGATTCTTCAATATGACGGTCCATTATTTTCAAGAATCGATACCCGTCAGATTGATG
ATGAAAGGGAAAGACTATTTCAGCATGCAATAGAAGCCAAATAA

Upstream 100 bases:

>100_bases
TAAGGTGGTACCGCGAGAATTCAAACCTCGCCCTTAACTGTTGTTATTGACAGTTAAGGGCGAGGTTTTTTCAATTTTAA
GGATGGGAGAGGTTCTAAGA

Downstream 100 bases:

>100_bases
AGTATAATCGGCTTAATCATTATTTAGTTATGAATGTCCTACGTGCCATATTTCGATAACACTTATAAACAGACAAAAAC
ATGACCCACCCTTCACGAGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSIFIGGAWVYANGSLHLGHISSLLSGDILARYYRSKGEDVLYVSGSDCNGTPITVRAKQEGVSVTEIADRYHDEFVACY
SSLGFSYDVYARTDHSQHHEVVQDIFAELYSKGFIYKKEVEQAYCETCDQFLPDRYVEGTCPVCHGPSRGDQCDHCSTIL
EPLDLLEPRCKHCLNEPSTRSTEHFYFSLSSFQKFLEKYTENADRQSLWRENAISLTKRYLQEGLHDRAVSRDLSTGVNI
PILGYEEKKVYVWIEAISGYYSASRLWAQSVGADEATFWSNTTRAYYVHGKDNIPFHSIIWPAILEALGDKALPTHIVSN
EYLTLEKRKLSTSRNWAVWLPDMLERYEPDSIRYFLTINAPENRDADFSWKEFIYSHNAELLGAYGNFVNRTLKFIEKSF
DHSIPNDNISSEIEERISNLYAEVGQLIEDTNFKQALELIFEAIRFSNKYFDQTQPWNTIKENPAECRQTLADCTFLIVN
FGNVLRPFLPFSSQKIRDAIEVDKEGWEVLKVKSSILQYDGPLFSRIDTRQIDDERERLFQHAIEAK

Sequences:

>Translated_547_residues
MSIFIGGAWVYANGSLHLGHISSLLSGDILARYYRSKGEDVLYVSGSDCNGTPITVRAKQEGVSVTEIADRYHDEFVACY
SSLGFSYDVYARTDHSQHHEVVQDIFAELYSKGFIYKKEVEQAYCETCDQFLPDRYVEGTCPVCHGPSRGDQCDHCSTIL
EPLDLLEPRCKHCLNEPSTRSTEHFYFSLSSFQKFLEKYTENADRQSLWRENAISLTKRYLQEGLHDRAVSRDLSTGVNI
PILGYEEKKVYVWIEAISGYYSASRLWAQSVGADEATFWSNTTRAYYVHGKDNIPFHSIIWPAILEALGDKALPTHIVSN
EYLTLEKRKLSTSRNWAVWLPDMLERYEPDSIRYFLTINAPENRDADFSWKEFIYSHNAELLGAYGNFVNRTLKFIEKSF
DHSIPNDNISSEIEERISNLYAEVGQLIEDTNFKQALELIFEAIRFSNKYFDQTQPWNTIKENPAECRQTLADCTFLIVN
FGNVLRPFLPFSSQKIRDAIEVDKEGWEVLKVKSSILQYDGPLFSRIDTRQIDDERERLFQHAIEAK
>Mature_546_residues
SIFIGGAWVYANGSLHLGHISSLLSGDILARYYRSKGEDVLYVSGSDCNGTPITVRAKQEGVSVTEIADRYHDEFVACYS
SLGFSYDVYARTDHSQHHEVVQDIFAELYSKGFIYKKEVEQAYCETCDQFLPDRYVEGTCPVCHGPSRGDQCDHCSTILE
PLDLLEPRCKHCLNEPSTRSTEHFYFSLSSFQKFLEKYTENADRQSLWRENAISLTKRYLQEGLHDRAVSRDLSTGVNIP
ILGYEEKKVYVWIEAISGYYSASRLWAQSVGADEATFWSNTTRAYYVHGKDNIPFHSIIWPAILEALGDKALPTHIVSNE
YLTLEKRKLSTSRNWAVWLPDMLERYEPDSIRYFLTINAPENRDADFSWKEFIYSHNAELLGAYGNFVNRTLKFIEKSFD
HSIPNDNISSEIEERISNLYAEVGQLIEDTNFKQALELIFEAIRFSNKYFDQTQPWNTIKENPAECRQTLADCTFLIVNF
GNVLRPFLPFSSQKIRDAIEVDKEGWEVLKVKSSILQYDGPLFSRIDTRQIDDERERLFQHAIEAK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=558, Percent_Identity=32.9749103942652, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI24308436, Length=517, Percent_Identity=26.6924564796905, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788432, Length=554, Percent_Identity=28.5198555956679, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1786861, Length=144, Percent_Identity=32.6388888888889, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=555, Percent_Identity=31.7117117117117, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=23.9766081871345, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71992781, Length=400, Percent_Identity=23.5, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=30.6737588652482, Blast_Score=296, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=24.6777163904236, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655311, Length=564, Percent_Identity=31.2056737588652, Blast_Score=301, Evalue=1e-81,
Organism=Drosophila melanogaster, GI21358483, Length=525, Percent_Identity=24.7619047619048, Blast_Score=129, Evalue=7e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 63053; Mature: 62922

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFIGGAWVYANGSLHLGHISSLLSGDILARYYRSKGEDVLYVSGSDCNGTPITVRAKQ
CEEEEEEEEEEECCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGVSVTEIADRYHDEFVACYSSLGFSYDVYARTDHSQHHEVVQDIFAELYSKGFIYKKEV
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEHHHH
EQAYCETCDQFLPDRYVEGTCPVCHGPSRGDQCDHCSTILEPLDLLEPRCKHCLNEPSTR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCC
STEHFYFSLSSFQKFLEKYTENADRQSLWRENAISLTKRYLQEGLHDRAVSRDLSTGVNI
CCHHHEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PILGYEEKKVYVWIEAISGYYSASRLWAQSVGADEATFWSNTTRAYYVHGKDNIPFHSII
EEEECCCCEEEEEEEHHHCHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCHHHHH
WPAILEALGDKALPTHIVSNEYLTLEKRKLSTSRNWAVWLPDMLERYEPDSIRYFLTINA
HHHHHHHHCCCCCCHHHCCCCEEEEEHHHCCCCCCEEEECHHHHHHCCCCCEEEEEEEEC
PENRDADFSWKEFIYSHNAELLGAYGNFVNRTLKFIEKSFDHSIPNDNISSEIEERISNL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
YAEVGQLIEDTNFKQALELIFEAIRFSNKYFDQTQPWNTIKENPAECRQTLADCTFLIVN
HHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
FGNVLRPFLPFSSQKIRDAIEVDKEGWEVLKVKSSILQYDGPLFSRIDTRQIDDERERLF
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHCCCHHHHHHH
QHAIEAK
HHHHCCC
>Mature Secondary Structure 
SIFIGGAWVYANGSLHLGHISSLLSGDILARYYRSKGEDVLYVSGSDCNGTPITVRAKQ
EEEEEEEEEEECCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGVSVTEIADRYHDEFVACYSSLGFSYDVYARTDHSQHHEVVQDIFAELYSKGFIYKKEV
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEHHHH
EQAYCETCDQFLPDRYVEGTCPVCHGPSRGDQCDHCSTILEPLDLLEPRCKHCLNEPSTR
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCC
STEHFYFSLSSFQKFLEKYTENADRQSLWRENAISLTKRYLQEGLHDRAVSRDLSTGVNI
CCHHHEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PILGYEEKKVYVWIEAISGYYSASRLWAQSVGADEATFWSNTTRAYYVHGKDNIPFHSII
EEEECCCCEEEEEEEHHHCHHHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCHHHHH
WPAILEALGDKALPTHIVSNEYLTLEKRKLSTSRNWAVWLPDMLERYEPDSIRYFLTINA
HHHHHHHHCCCCCCHHHCCCCEEEEEHHHCCCCCCEEEECHHHHHHCCCCCEEEEEEEEC
PENRDADFSWKEFIYSHNAELLGAYGNFVNRTLKFIEKSFDHSIPNDNISSEIEERISNL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
YAEVGQLIEDTNFKQALELIFEAIRFSNKYFDQTQPWNTIKENPAECRQTLADCTFLIVN
HHHHHHHHHCCCHHHHHHHHHHHHHHCCHHCCCCCCCCHHCCCHHHHHHHHHHHHHHHHH
FGNVLRPFLPFSSQKIRDAIEVDKEGWEVLKVKSSILQYDGPLFSRIDTRQIDDERERLF
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCHHCCCHHHHHHH
QHAIEAK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA