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The map label for this gene is sbcD [H]

Identifier: 229916110

GI number: 229916110

Start: 341317

End: 342420

Strand: Reverse

Name: sbcD [H]

Synonym: EAT1b_0379

Alternate gene names: 229916110

Gene position: 342420-341317 (Counterclockwise)

Preceding gene: 229916114

Following gene: 229916109

Centisome position: 11.41

GC content: 48.28

Gene sequence:

>1104_bases
ATGAAATGGATTCACACGGCCGATTGGCATCTAGGAAAGATTGTCCACGGTCGACATATGACAGAAGATCAACGGAATAT
CTTGTTCCATTCATTTTTACAAATCGTAGATGAAGAGCGACCCGATGCGGTCATCATCGCAGGTGATTTATATGACCGTG
CCGTCCCTCCCGTCGAGGCGGTCGATTTACTCGATGAGATGTGGCGAGAGCTCGTCTTGAACCGCGGAATCCCAGTCGTA
GCCATCGCAGGAAACCATGATTCGGCAGAACGTCTCGAATATAGTTCGAAGTTATTGAGACAAGTAGGACTTCACATCGT
CGGGAAGCTAGACCCGACATCTGGTCCCCTTGATGTCGCAGGGGTCCCATTTTATCCGATTCCATTCCTTGAGCCGGCTC
GCGTACGACATATCCTCGGTATCGACTCGATTCGAACGCATCACGATGCACTGGAGGCAGTCATTGAAGCTTACGGTACA
ATCGAAAAAGATGCGGTTACAATCGGTCACAGTTTTGTCGCGGGCGGACTGGAGACGGATTCAGAGCGTCAGTTATCCGT
TGGCACGGCTGGACAGGTCGCAAAAGGGTTATATGAGCCTTTTTCGTATACGGCATTAGGTCATTTACATAATCGTGATG
CGATTCAATCAGAAAGAATTGCATATAGCGGGTCGCTCATGAAATATTCGTTTTCCGAGGTCAATCATGAAAAGTCCGTC
GATATTCTGGAATGGAACGGTCAATGGACACGTCGTCGCCGTGCCTTGACACCAGAGCGAGATATGCGACAACTCACGGG
AACATTGAACGAACTCCTTGCCCCAACTTTTTATCAGCAACAACAGACAGATGACTACCTTAAAATTGTATTGACCGACG
ATACGGCGCTCCATGATCCGATGGCGAAACTCCGGACTGTCTATCCGAATATCCTTCATCTTGAACTGGAACTGTTACGT
CGTCAGGAACAGACATCCACGTTTGAACGCGAAACGCTCGAAAGACAGTCCGTAGAAGAGGTATATCTTCAATTTTTTGA
GGCCGTTCATGATTGCACTGCACCAGATGCCGTGAAGCAAATGATAGAAGAGGGTGATGTCTGA

Upstream 100 bases:

>100_bases
GTCCTCCTCTCCTCTTACTTTCGCTGTTTCAAAAACGGGTGTCAAGACGTCGAATCATACGAATGACTCATGCTAGAATA
GGAAAGAGAGGTGATACGTG

Downstream 100 bases:

>100_bases
TGAGACCGGTCGAATTGATGATTCAAGCATTCGGACCATATGCAGAACGTGAAACGATTGACTTCACCCAGTTAGTTGGC
CGCTCGATGTTTGTCGTCAG

Product: nuclease SbcCD, D subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKWIHTADWHLGKIVHGRHMTEDQRNILFHSFLQIVDEERPDAVIIAGDLYDRAVPPVEAVDLLDEMWRELVLNRGIPVV
AIAGNHDSAERLEYSSKLLRQVGLHIVGKLDPTSGPLDVAGVPFYPIPFLEPARVRHILGIDSIRTHHDALEAVIEAYGT
IEKDAVTIGHSFVAGGLETDSERQLSVGTAGQVAKGLYEPFSYTALGHLHNRDAIQSERIAYSGSLMKYSFSEVNHEKSV
DILEWNGQWTRRRRALTPERDMRQLTGTLNELLAPTFYQQQQTDDYLKIVLTDDTALHDPMAKLRTVYPNILHLELELLR
RQEQTSTFERETLERQSVEEVYLQFFEAVHDCTAPDAVKQMIEEGDV

Sequences:

>Translated_367_residues
MKWIHTADWHLGKIVHGRHMTEDQRNILFHSFLQIVDEERPDAVIIAGDLYDRAVPPVEAVDLLDEMWRELVLNRGIPVV
AIAGNHDSAERLEYSSKLLRQVGLHIVGKLDPTSGPLDVAGVPFYPIPFLEPARVRHILGIDSIRTHHDALEAVIEAYGT
IEKDAVTIGHSFVAGGLETDSERQLSVGTAGQVAKGLYEPFSYTALGHLHNRDAIQSERIAYSGSLMKYSFSEVNHEKSV
DILEWNGQWTRRRRALTPERDMRQLTGTLNELLAPTFYQQQQTDDYLKIVLTDDTALHDPMAKLRTVYPNILHLELELLR
RQEQTSTFERETLERQSVEEVYLQFFEAVHDCTAPDAVKQMIEEGDV
>Mature_367_residues
MKWIHTADWHLGKIVHGRHMTEDQRNILFHSFLQIVDEERPDAVIIAGDLYDRAVPPVEAVDLLDEMWRELVLNRGIPVV
AIAGNHDSAERLEYSSKLLRQVGLHIVGKLDPTSGPLDVAGVPFYPIPFLEPARVRHILGIDSIRTHHDALEAVIEAYGT
IEKDAVTIGHSFVAGGLETDSERQLSVGTAGQVAKGLYEPFSYTALGHLHNRDAIQSERIAYSGSLMKYSFSEVNHEKSV
DILEWNGQWTRRRRALTPERDMRQLTGTLNELLAPTFYQQQQTDDYLKIVLTDDTALHDPMAKLRTVYPNILHLELELLR
RQEQTSTFERETLERQSVEEVYLQFFEAVHDCTAPDAVKQMIEEGDV

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=379, Percent_Identity=28.4960422163588, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: 3.1.11.-

Molecular weight: Translated: 41721; Mature: 41721

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWIHTADWHLGKIVHGRHMTEDQRNILFHSFLQIVDEERPDAVIIAGDLYDRAVPPVEA
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCHHH
VDLLDEMWRELVLNRGIPVVAIAGNHDSAERLEYSSKLLRQVGLHIVGKLDPTSGPLDVA
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCC
GVPFYPIPFLEPARVRHILGIDSIRTHHDALEAVIEAYGTIEKDAVTIGHSFVAGGLETD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCHHHHCCCCCCC
SERQLSVGTAGQVAKGLYEPFSYTALGHLHNRDAIQSERIAYSGSLMKYSFSEVNHEKSV
CCCEEECCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCHHEEHHHHCCCCCCC
DILEWNGQWTRRRRALTPERDMRQLTGTLNELLAPTFYQQQQTDDYLKIVLTDDTALHDP
EEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCHHHHH
MAKLRTVYPNILHLELELLRRQEQTSTFERETLERQSVEEVYLQFFEAVHDCTAPDAVKQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MIEEGDV
HHHHCCC
>Mature Secondary Structure
MKWIHTADWHLGKIVHGRHMTEDQRNILFHSFLQIVDEERPDAVIIAGDLYDRAVPPVEA
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCHHH
VDLLDEMWRELVLNRGIPVVAIAGNHDSAERLEYSSKLLRQVGLHIVGKLDPTSGPLDVA
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCC
GVPFYPIPFLEPARVRHILGIDSIRTHHDALEAVIEAYGTIEKDAVTIGHSFVAGGLETD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCHHHHCCCCCCC
SERQLSVGTAGQVAKGLYEPFSYTALGHLHNRDAIQSERIAYSGSLMKYSFSEVNHEKSV
CCCEEECCCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCHHEEHHHHCCCCCCC
DILEWNGQWTRRRRALTPERDMRQLTGTLNELLAPTFYQQQQTDDYLKIVLTDDTALHDP
EEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCHHHHH
MAKLRTVYPNILHLELELLRRQEQTSTFERETLERQSVEEVYLQFFEAVHDCTAPDAVKQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MIEEGDV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA