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The map label for this gene is alsT [H]

Identifier: 229916048

GI number: 229916048

Start: 284314

End: 285732

Strand: Reverse

Name: alsT [H]

Synonym: EAT1b_0316

Alternate gene names: 229916048

Gene position: 285732-284314 (Counterclockwise)

Preceding gene: 229916049

Following gene: 229916047

Centisome position: 9.52

GC content: 49.47

Gene sequence:

>1419_bases
TTGGATATTCGTGAACTGATTATGCAAATCAATGACTATTTATGGTCGTACCTTCTCATCTTCGTATTGATTGCAGGAGG
TCTTTATTTTACGATTCGGATGGGGTTCGTTCAATTTCGAATGTTACCGGAAATGTTTAAACTTTTATTCGTGAAAGGAA
TCAAGCATGAGAAAGGACAGGTCTCATCTTTCCAAGCATTCGCCATCGGAACGGCGGCCCGTGTCGGTACGGGGAACATC
GCCGGCGTATCGACGGCAATCGCTTTAGGCGGACCTGGCGCCGTATTTTGGATGTGGCTCATTGCACTGATTGGAGCGGC
GTCGGCATTCGTTGAAAGTACGCTCGCCCAGGTGTATAAAGTGCATGACGGCAAGGCGTGGCGTGGTGGTCCGGCATACT
ATATTGAGCGCGCTCTCGGTGCACGCTGGTTAGGCATCGTATTTGCCATTTTGATTACGTTCGCCTTCGGTTTTGCCTTC
AACTCCGTCCAGGCGAATACAATCGCACTCTCCGTGAATAACGTATTTGAGATCGATACGGTCTGGGTCGGCATCATCTT
ATCCGTTGTCACCGCTATCGTCATCTTCGGTGGGATTCGTTCCATCTCGACCTTATCGGGAATCATTGTACCAATCATGG
CGGGCGGTTATATCTTACTAGCCATTTGGGTCGTCCTTACGAATCTTGATGTCGTGCCACAAGTGTTCTCGGCCATCTTC
TCTGAAGGTGTCCTCGGTTTCCAACAGTTGTTCGGTGGAGCGGTCGGTGCAGCTGTCCTCCAAGGTATTCGACGAGGCCT
GTTCTCGAACGAGGCAGGGATGGGGTCTGCCCCGAACGCCGCAGCTACCGCTGAGGTGAGTCACCCGGTCAAACAAGGAT
TGATTCAATCGCTTAGTGTATTTGTGGACACATTGTTCATCTGTACCGCAACGGCGATGATTGTCCTCATCTCAGGTGTC
GAAGGCTCTGAAGCATTCCAAGGGATCGAGCTTGCCCAAGAGGCGCTTCTCGTCGAAATTGGACCGATTGCGACGACGCT
CATGACAATCGCCATCTTACTCTTTGCCTTCAGTTCGATTTTGGCCAATTATTATTATGGGGAGACGAACATCGAGTTCA
TTTCTGAGAAGAAGCGGTATTTGATTATCTATCGGATTGCCGTTGTCGGGATGGTCATGTTCGGGTCAGTTCGATCGGCG
GGACTCGTCTGGTCGATTGCCGACATCTTTATGGGGCTGATGGCGCTCGTCAACATGTATGCGATCTTCCGATTGAGTAA
AGTCGCCAAGACGCTCCTCGATGACTATATGGTTCAAAAGAAAGAAGGAAGAGACCCGGAATTCTTTAAAGATGGTGTCG
ATCTTCCTGGTAAAGAGCACATCGAAGCGTGGGAAACGAAAAAAGAAGAAATTAGTTAA

Upstream 100 bases:

>100_bases
CACATCGTCTATGTGAAGAGATTCTTAAAAACGTGCAATGAACCCGCTGTTTCTGTCAGATTCAGAGCGGGTAAAAACCC
GATAAAAGGAGGCGATTTCG

Downstream 100 bases:

>100_bases
AGGATTATCATTGGGACGCCCCTGGCATCAGGGGTTTCCTCTTCATTTTAAATTATTTATTTCAAATACCGCTATTATTT
TATTAAAAGAAAGTAGAGAG

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDIRELIMQINDYLWSYLLIFVLIAGGLYFTIRMGFVQFRMLPEMFKLLFVKGIKHEKGQVSSFQAFAIGTAARVGTGNI
AGVSTAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYKVHDGKAWRGGPAYYIERALGARWLGIVFAILITFAFGFAF
NSVQANTIALSVNNVFEIDTVWVGIILSVVTAIVIFGGIRSISTLSGIIVPIMAGGYILLAIWVVLTNLDVVPQVFSAIF
SEGVLGFQQLFGGAVGAAVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSLSVFVDTLFICTATAMIVLISGV
EGSEAFQGIELAQEALLVEIGPIATTLMTIAILLFAFSSILANYYYGETNIEFISEKKRYLIIYRIAVVGMVMFGSVRSA
GLVWSIADIFMGLMALVNMYAIFRLSKVAKTLLDDYMVQKKEGRDPEFFKDGVDLPGKEHIEAWETKKEEIS

Sequences:

>Translated_472_residues
MDIRELIMQINDYLWSYLLIFVLIAGGLYFTIRMGFVQFRMLPEMFKLLFVKGIKHEKGQVSSFQAFAIGTAARVGTGNI
AGVSTAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYKVHDGKAWRGGPAYYIERALGARWLGIVFAILITFAFGFAF
NSVQANTIALSVNNVFEIDTVWVGIILSVVTAIVIFGGIRSISTLSGIIVPIMAGGYILLAIWVVLTNLDVVPQVFSAIF
SEGVLGFQQLFGGAVGAAVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSLSVFVDTLFICTATAMIVLISGV
EGSEAFQGIELAQEALLVEIGPIATTLMTIAILLFAFSSILANYYYGETNIEFISEKKRYLIIYRIAVVGMVMFGSVRSA
GLVWSIADIFMGLMALVNMYAIFRLSKVAKTLLDDYMVQKKEGRDPEFFKDGVDLPGKEHIEAWETKKEEIS
>Mature_472_residues
MDIRELIMQINDYLWSYLLIFVLIAGGLYFTIRMGFVQFRMLPEMFKLLFVKGIKHEKGQVSSFQAFAIGTAARVGTGNI
AGVSTAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYKVHDGKAWRGGPAYYIERALGARWLGIVFAILITFAFGFAF
NSVQANTIALSVNNVFEIDTVWVGIILSVVTAIVIFGGIRSISTLSGIIVPIMAGGYILLAIWVVLTNLDVVPQVFSAIF
SEGVLGFQQLFGGAVGAAVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSLSVFVDTLFICTATAMIVLISGV
EGSEAFQGIELAQEALLVEIGPIATTLMTIAILLFAFSSILANYYYGETNIEFISEKKRYLIIYRIAVVGMVMFGSVRSA
GLVWSIADIFMGLMALVNMYAIFRLSKVAKTLLDDYMVQKKEGRDPEFFKDGVDLPGKEHIEAWETKKEEIS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=474, Percent_Identity=40.9282700421941, Blast_Score=327, Evalue=7e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51215; Mature: 51215

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRELIMQINDYLWSYLLIFVLIAGGLYFTIRMGFVQFRMLPEMFKLLFVKGIKHEKGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSSFQAFAIGTAARVGTGNIAGVSTAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYK
CCCHHHHHHHCHHCCCCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHDGKAWRGGPAYYIERALGARWLGIVFAILITFAFGFAFNSVQANTIALSVNNVFEIDT
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
VWVGIILSVVTAIVIFGGIRSISTLSGIIVPIMAGGYILLAIWVVLTNLDVVPQVFSAIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHH
SEGVLGFQQLFGGAVGAAVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSLSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FVDTLFICTATAMIVLISGVEGSEAFQGIELAQEALLVEIGPIATTLMTIAILLFAFSSI
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANYYYGETNIEFISEKKRYLIIYRIAVVGMVMFGSVRSAGLVWSIADIFMGLMALVNMY
HHHHHCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIFRLSKVAKTLLDDYMVQKKEGRDPEFFKDGVDLPGKEHIEAWETKKEEIS
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIRELIMQINDYLWSYLLIFVLIAGGLYFTIRMGFVQFRMLPEMFKLLFVKGIKHEKGQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSSFQAFAIGTAARVGTGNIAGVSTAIALGGPGAVFWMWLIALIGAASAFVESTLAQVYK
CCCHHHHHHHCHHCCCCCCCCHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VHDGKAWRGGPAYYIERALGARWLGIVFAILITFAFGFAFNSVQANTIALSVNNVFEIDT
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
VWVGIILSVVTAIVIFGGIRSISTLSGIIVPIMAGGYILLAIWVVLTNLDVVPQVFSAIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHH
SEGVLGFQQLFGGAVGAAVLQGIRRGLFSNEAGMGSAPNAAATAEVSHPVKQGLIQSLSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FVDTLFICTATAMIVLISGVEGSEAFQGIELAQEALLVEIGPIATTLMTIAILLFAFSSI
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANYYYGETNIEFISEKKRYLIIYRIAVVGMVMFGSVRSAGLVWSIADIFMGLMALVNMY
HHHHHCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AIFRLSKVAKTLLDDYMVQKKEGRDPEFFKDGVDLPGKEHIEAWETKKEEIS
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]