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The map label for this gene is parE [H]

Identifier: 229916010

GI number: 229916010

Start: 244162

End: 246129

Strand: Reverse

Name: parE [H]

Synonym: EAT1b_0278

Alternate gene names: 229916010

Gene position: 246129-244162 (Counterclockwise)

Preceding gene: 229916011

Following gene: 229916009

Centisome position: 8.2

GC content: 54.78

Gene sequence:

>1968_bases
ATGTCAACAGATCTATTTAATTACAATGAAGACGCCATCCAGGTGCTCGAAGGACTCACGGCCGTACGGAAACGTCCGGG
GATGTACATCGGGTCAACCGACCATCGCGGACTTCACCATCTCGTCTATGAGATCGTCGACAACGCGATCGATGAGGCAC
TCGCAGGCTTTGGCGGTCAAATCGATGTGACGATTCATAAAAATGATGCCATCACGGTCCGTGACCACGGGCGTGGGATG
CCGACAGGAATGCACGCCTCTGGAAAGCCTACCCCAGAAGTCATCTTCACGGTCCTCCACGCCGGCGGGAAGTTCGGCCA
AGGCGGCTACAAGACGTCGGGTGGACTTCACGGCGTCGGCGCCTCAGTCGTCAACGCCCTCTCGAGCAAGGTCGTCGTGA
CGATTCACCGTGACGGCAAAGTGTTTGAACAGACTTTCGCGGATGGCGGTGTGCCTCAAACGACACTGATTGAGACAGGC
AAGACACGCCAGACGGGCACGAGCGTCTATTTCAAACCGGATGCGACCATCTTCTCGACGACGACATATAACCATGACAC
GCTCGCCGAACGCCTCCGTGAATCGGCCTTCCTCTTAAAAGGATTGACGATCACGCTCACAGACGAGCGTACGGAGAAAC
AAGAGACGTTCCATTACGAGGAAGGAATCGCCGAGTTCGTCCAATATTTGAACGACGACAAGGCGACGCTTCATCCGATC
GTCTATTTCGAAGGGACAGAGAACGAGATCGAGATCGAACTCGCGTTTCAGTTCACGGATGCCTATTCCGAAAATGTGCT
CTCGTTCGTCAACAACGTCCGGACACGCGATGGTGGAACGCACGAGGTCGGTGCCAAAACGGCGATGACCCGGATCATGA
ATGAGTATGCCCGTAAGAACGGACTGCTCAAAGAGAAGGATAAGAACCTAGACGGCGGTGATGTCCGTGAAGGACTCACG
CTCATCGTCTCGGTACGGATTCCGGAGGAGTTCCTCCAGTTCGAGGGGCAGACGAAATCGAAGCTTGGTTCCCCGGAGGC
GCGTGCGAGTGCCGATAGCGTCATGGCGAAGCAACTGACGATCTTCTTAGAAGAGAACCCACAGATGGCGACAATGCTCA
TCAAGAAGGCGATTCGTGCGGCTCAGGCTCGCGAGGCGGCTCGAAAGGCCCGTGAAGAGGCACGTAACGGGAAGAAAAAG
AAACGTTCGAGCATCTTGAACGGGAAATTGACGCCGGCGACGTCGAAGAATGCCGCCAAGAACGAACTGTTCCTCGTCGA
GGGTGATTCGGCCGGTGGTTCCGCCAAACAAGGGCGCGACCGTACGTTCCAAGCCATCCTCCCACTACGCGGGAAGGTCA
TCAACTCAGAGAAGTCGAAACTTCAGGACATCATGAAAAACGAAGAGATTAACACGATCATCCACGCGATCGGTGCCGGG
GTCGGTCATGATTTCGACTTGGATGACTGCAACTTCGATAAGATCATCATCATGACGGATGCCGATACGGACGGTGCGCA
TATCCAAGTGCTCCTTCTCACTTTCTTCTTCCGTTATATGCGACCGCTCGTCGAGGCGGGCCGTGTGTTCGTCGCCTTGC
CACCGCTCTATCGCATCTCAAAAGGGAAAGGCAAGTCCGAACAGTTCGACTATGCCTGGGACGAAGATGCGCTCGAGAAG
CTCGTCAAAGTGTATAAGAAAGGCTATATCCTCCAACGCTACAAGGGTCTCGGTGAGATGAACGCGGACCAGTTATGGGA
GACGACGATGAATCCGGATACCCGTACGCTCATCCGTGTCACGGTGGACGACGCGGCTGTCGCGGACAAGCGTGTATCGG
TCTTGATGGGTGACAACGTCACGCATCGCCGTGAGTGGATCGAGGAGAACGTCGCCTTCGGACTCCAGGAAGATGATTCG
ATCATCGCCAATGAACATGTGACTAAAGCGATTGAGGAAGTGATGTAA

Upstream 100 bases:

>100_bases
TTTTTCAGCGATGGCTGACAAGGGAGGCTTGCCAGAATCGATGAACAGCTATACAATAAGAAAGAAAAACGAACGTGTGT
TCGAAATTGAGGTGACTGCT

Downstream 100 bases:

>100_bases
CGATGTCGACGATTCAACACATTTTAAACCTATCTCTCGAACAGGTCGTCGGTGACCGGTTCGGGCGTTACTCAAAATAT
ATCATTCAAGACCGTGCCTT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSTDLFNYNEDAIQVLEGLTAVRKRPGMYIGSTDHRGLHHLVYEIVDNAIDEALAGFGGQIDVTIHKNDAITVRDHGRGM
PTGMHASGKPTPEVIFTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSSKVVVTIHRDGKVFEQTFADGGVPQTTLIETG
KTRQTGTSVYFKPDATIFSTTTYNHDTLAERLRESAFLLKGLTITLTDERTEKQETFHYEEGIAEFVQYLNDDKATLHPI
VYFEGTENEIEIELAFQFTDAYSENVLSFVNNVRTRDGGTHEVGAKTAMTRIMNEYARKNGLLKEKDKNLDGGDVREGLT
LIVSVRIPEEFLQFEGQTKSKLGSPEARASADSVMAKQLTIFLEENPQMATMLIKKAIRAAQAREAARKAREEARNGKKK
KRSSILNGKLTPATSKNAAKNELFLVEGDSAGGSAKQGRDRTFQAILPLRGKVINSEKSKLQDIMKNEEINTIIHAIGAG
VGHDFDLDDCNFDKIIIMTDADTDGAHIQVLLLTFFFRYMRPLVEAGRVFVALPPLYRISKGKGKSEQFDYAWDEDALEK
LVKVYKKGYILQRYKGLGEMNADQLWETTMNPDTRTLIRVTVDDAAVADKRVSVLMGDNVTHRREWIEENVAFGLQEDDS
IIANEHVTKAIEEVM

Sequences:

>Translated_655_residues
MSTDLFNYNEDAIQVLEGLTAVRKRPGMYIGSTDHRGLHHLVYEIVDNAIDEALAGFGGQIDVTIHKNDAITVRDHGRGM
PTGMHASGKPTPEVIFTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSSKVVVTIHRDGKVFEQTFADGGVPQTTLIETG
KTRQTGTSVYFKPDATIFSTTTYNHDTLAERLRESAFLLKGLTITLTDERTEKQETFHYEEGIAEFVQYLNDDKATLHPI
VYFEGTENEIEIELAFQFTDAYSENVLSFVNNVRTRDGGTHEVGAKTAMTRIMNEYARKNGLLKEKDKNLDGGDVREGLT
LIVSVRIPEEFLQFEGQTKSKLGSPEARASADSVMAKQLTIFLEENPQMATMLIKKAIRAAQAREAARKAREEARNGKKK
KRSSILNGKLTPATSKNAAKNELFLVEGDSAGGSAKQGRDRTFQAILPLRGKVINSEKSKLQDIMKNEEINTIIHAIGAG
VGHDFDLDDCNFDKIIIMTDADTDGAHIQVLLLTFFFRYMRPLVEAGRVFVALPPLYRISKGKGKSEQFDYAWDEDALEK
LVKVYKKGYILQRYKGLGEMNADQLWETTMNPDTRTLIRVTVDDAAVADKRVSVLMGDNVTHRREWIEENVAFGLQEDDS
IIANEHVTKAIEEVM
>Mature_654_residues
STDLFNYNEDAIQVLEGLTAVRKRPGMYIGSTDHRGLHHLVYEIVDNAIDEALAGFGGQIDVTIHKNDAITVRDHGRGMP
TGMHASGKPTPEVIFTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSSKVVVTIHRDGKVFEQTFADGGVPQTTLIETGK
TRQTGTSVYFKPDATIFSTTTYNHDTLAERLRESAFLLKGLTITLTDERTEKQETFHYEEGIAEFVQYLNDDKATLHPIV
YFEGTENEIEIELAFQFTDAYSENVLSFVNNVRTRDGGTHEVGAKTAMTRIMNEYARKNGLLKEKDKNLDGGDVREGLTL
IVSVRIPEEFLQFEGQTKSKLGSPEARASADSVMAKQLTIFLEENPQMATMLIKKAIRAAQAREAARKAREEARNGKKKK
RSSILNGKLTPATSKNAAKNELFLVEGDSAGGSAKQGRDRTFQAILPLRGKVINSEKSKLQDIMKNEEINTIIHAIGAGV
GHDFDLDDCNFDKIIIMTDADTDGAHIQVLLLTFFFRYMRPLVEAGRVFVALPPLYRISKGKGKSEQFDYAWDEDALEKL
VKVYKKGYILQRYKGLGEMNADQLWETTMNPDTRTLIRVTVDDAAVADKRVSVLMGDNVTHRREWIEENVAFGLQEDDSI
IANEHVTKAIEEVM

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=576, Percent_Identity=26.0416666666667, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI19913408, Length=634, Percent_Identity=24.7634069400631, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI48994957, Length=559, Percent_Identity=49.7316636851521, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1789408, Length=633, Percent_Identity=39.652448657188, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17535065, Length=665, Percent_Identity=24.2105263157895, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=611, Percent_Identity=24.5499181669394, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=199, Percent_Identity=28.643216080402, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=684, Percent_Identity=26.1695906432749, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17136538, Length=627, Percent_Identity=25.1993620414673, Blast_Score=123, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 72677; Mature: 72546

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDLFNYNEDAIQVLEGLTAVRKRPGMYIGSTDHRGLHHLVYEIVDNAIDEALAGFGGQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
IDVTIHKNDAITVRDHGRGMPTGMHASGKPTPEVIFTVLHAGGKFGQGGYKTSGGLHGVG
EEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ASVVNALSSKVVVTIHRDGKVFEQTFADGGVPQTTLIETGKTRQTGTSVYFKPDATIFST
HHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCEEEEE
TTYNHDTLAERLRESAFLLKGLTITLTDERTEKQETFHYEEGIAEFVQYLNDDKATLHPI
ECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VYFEGTENEIEIELAFQFTDAYSENVLSFVNNVRTRDGGTHEVGAKTAMTRIMNEYARKN
EEEECCCCEEEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
GLLKEKDKNLDGGDVREGLTLIVSVRIPEEFLQFEGQTKSKLGSPEARASADSVMAKQLT
CCCCCCCCCCCCCHHHCCEEEEEEEECCHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHEE
IFLEENPQMATMLIKKAIRAAQAREAARKAREEARNGKKKKRSSILNGKLTPATSKNAAK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCC
NELFLVEGDSAGGSAKQGRDRTFQAILPLRGKVINSEKSKLQDIMKNEEINTIIHAIGAG
CEEEEEECCCCCCCCCCCCCCHHHHHHCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHCC
VGHDFDLDDCNFDKIIIMTDADTDGAHIQVLLLTFFFRYMRPLVEAGRVFVALPPLYRIS
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHEEC
KGKGKSEQFDYAWDEDALEKLVKVYKKGYILQRYKGLGEMNADQLWETTMNPDTRTLIRV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEE
TVDDAAVADKRVSVLMGDNVTHRREWIEENVAFGLQEDDSIIANEHVTKAIEEVM
EECCHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
STDLFNYNEDAIQVLEGLTAVRKRPGMYIGSTDHRGLHHLVYEIVDNAIDEALAGFGGQ
CCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE
IDVTIHKNDAITVRDHGRGMPTGMHASGKPTPEVIFTVLHAGGKFGQGGYKTSGGLHGVG
EEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
ASVVNALSSKVVVTIHRDGKVFEQTFADGGVPQTTLIETGKTRQTGTSVYFKPDATIFST
HHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEECCCCEEEEE
TTYNHDTLAERLRESAFLLKGLTITLTDERTEKQETFHYEEGIAEFVQYLNDDKATLHPI
ECCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VYFEGTENEIEIELAFQFTDAYSENVLSFVNNVRTRDGGTHEVGAKTAMTRIMNEYARKN
EEEECCCCEEEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
GLLKEKDKNLDGGDVREGLTLIVSVRIPEEFLQFEGQTKSKLGSPEARASADSVMAKQLT
CCCCCCCCCCCCCHHHCCEEEEEEEECCHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHEE
IFLEENPQMATMLIKKAIRAAQAREAARKAREEARNGKKKKRSSILNGKLTPATSKNAAK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCC
NELFLVEGDSAGGSAKQGRDRTFQAILPLRGKVINSEKSKLQDIMKNEEINTIIHAIGAG
CEEEEEECCCCCCCCCCCCCCHHHHHHCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHCC
VGHDFDLDDCNFDKIIIMTDADTDGAHIQVLLLTFFFRYMRPLVEAGRVFVALPPLYRIS
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHEEC
KGKGKSEQFDYAWDEDALEKLVKVYKKGYILQRYKGLGEMNADQLWETTMNPDTRTLIRV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEE
TVDDAAVADKRVSVLMGDNVTHRREWIEENVAFGLQEDDSIIANEHVTKAIEEVM
EECCHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377 [H]