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The map label for this gene is ywbI [H]

Identifier: 229915962

GI number: 229915962

Start: 199357

End: 200241

Strand: Reverse

Name: ywbI [H]

Synonym: EAT1b_0230

Alternate gene names: 229915962

Gene position: 200241-199357 (Counterclockwise)

Preceding gene: 229915964

Following gene: 229915961

Centisome position: 6.67

GC content: 46.1

Gene sequence:

>885_bases
ATGGAATTCCGAGAGATGCAATACTTCATAGCGGTTGCACGAGCCAAGAGTTTCACGCGAGCAGCCGAAGCGCTACACGT
CACACAACCTACGTTGAGTAAAACGATTCGACACTTAGAACAAGAACTCGGGGTCGAACTGTTCGATCGCCAGAGACGGC
AAATTGAATTAACAGATGTGGGAGAAGCCTTGATGCTTGAGAGCGAGCTATTAATACGTCAGCTCGAGGACTTACCGAAT
CACGTTTTCGATATCACCAATTCCAAGCGAGGGCTCATTCGACTTGGTCTCCCCCCGCTAATCGGTTCATTATTTTTCAC
GAAGTTCTTGACTGTATTCCATTCAAGCTATCCGAACATTCGTGTTGAGATTACAGAAGCCGGTGGACACACGCTTGAAC
ACGCACTCGAAAAAGGAGAATTAGATGTGATTGCAACTGTTTTACCGACAAGTTCCACGTTTGAAGAAACTCCTTTTGTC
ACTGAGGAACTGTATGCTTACTTGCCCGAACGTCACCCTTTAGCATCACGTAAACAACTCCTGCTCGAAGAACTCGCATC
TGAGCCTTTCGTCTTGTTTACAGATGAGTTTTCGCTCCATCACGTCGTTCGAACTGCCTGTGAACAAGCGGGATTCACCC
CCCGACTTATCGGGGAAAGTGGGCAATGGGATTTTTTATGCATGTCCGTCGCACAAGGTAACGCGGTAACCATATTACCT
CGCTCCATTATGCAAGAGATGAATATATATGGGGTAAAGGCGATTTTACTCAAACAAAGGATTCCGTGGCAACTTGGATT
AGCGACTGCAAAAGGCAGATATTTACCGTATGCCACGAGAGAGTTTATCCGTTACTTGACCGAAGAGTATTCGTTTGATG
CATAA

Upstream 100 bases:

>100_bases
TTCATTGACCTTTACAAACTTCATTGTATACTTAGTTAAATGATAGTAAAAATGAATATTTATTATACAAACCATGCCAC
GATAGAATAGGATGAGCAGA

Downstream 100 bases:

>100_bases
CTGATATAAAAAAGATGAATTGGAAGCATGGCAAAAGAATTGCTACGATGGATTCAGCTTCAATAGAGAGGATGAATCGA
AAATGGAAACAAGACATGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEFREMQYFIAVARAKSFTRAAEALHVTQPTLSKTIRHLEQELGVELFDRQRRQIELTDVGEALMLESELLIRQLEDLPN
HVFDITNSKRGLIRLGLPPLIGSLFFTKFLTVFHSSYPNIRVEITEAGGHTLEHALEKGELDVIATVLPTSSTFEETPFV
TEELYAYLPERHPLASRKQLLLEELASEPFVLFTDEFSLHHVVRTACEQAGFTPRLIGESGQWDFLCMSVAQGNAVTILP
RSIMQEMNIYGVKAILLKQRIPWQLGLATAKGRYLPYATREFIRYLTEEYSFDA

Sequences:

>Translated_294_residues
MEFREMQYFIAVARAKSFTRAAEALHVTQPTLSKTIRHLEQELGVELFDRQRRQIELTDVGEALMLESELLIRQLEDLPN
HVFDITNSKRGLIRLGLPPLIGSLFFTKFLTVFHSSYPNIRVEITEAGGHTLEHALEKGELDVIATVLPTSSTFEETPFV
TEELYAYLPERHPLASRKQLLLEELASEPFVLFTDEFSLHHVVRTACEQAGFTPRLIGESGQWDFLCMSVAQGNAVTILP
RSIMQEMNIYGVKAILLKQRIPWQLGLATAKGRYLPYATREFIRYLTEEYSFDA
>Mature_294_residues
MEFREMQYFIAVARAKSFTRAAEALHVTQPTLSKTIRHLEQELGVELFDRQRRQIELTDVGEALMLESELLIRQLEDLPN
HVFDITNSKRGLIRLGLPPLIGSLFFTKFLTVFHSSYPNIRVEITEAGGHTLEHALEKGELDVIATVLPTSSTFEETPFV
TEELYAYLPERHPLASRKQLLLEELASEPFVLFTDEFSLHHVVRTACEQAGFTPRLIGESGQWDFLCMSVAQGNAVTILP
RSIMQEMNIYGVKAILLKQRIPWQLGLATAKGRYLPYATREFIRYLTEEYSFDA

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=34.2756183745583, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790399, Length=241, Percent_Identity=30.7053941908714, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=31.6, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=31.0810810810811, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI2367136, Length=274, Percent_Identity=32.4817518248175, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI145693105, Length=187, Percent_Identity=31.0160427807487, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=26.9387755102041, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790262, Length=254, Percent_Identity=25.9842519685039, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787530, Length=243, Percent_Identity=23.8683127572016, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=27.3255813953488, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789506, Length=134, Percent_Identity=31.3432835820896, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=240, Percent_Identity=24.1666666666667, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787806, Length=265, Percent_Identity=23.3962264150943, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788296, Length=243, Percent_Identity=22.6337448559671, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788748, Length=239, Percent_Identity=23.8493723849372, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789173, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787128, Length=203, Percent_Identity=24.6305418719212, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786401, Length=123, Percent_Identity=26.8292682926829, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=191, Percent_Identity=26.1780104712042, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081904, Length=147, Percent_Identity=29.2517006802721, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=285, Percent_Identity=22.8070175438596, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994962, Length=177, Percent_Identity=28.2485875706215, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33606; Mature: 33606

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFREMQYFIAVARAKSFTRAAEALHVTQPTLSKTIRHLEQELGVELFDRQRRQIELTDV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHEEEHHHC
GEALMLESELLIRQLEDLPNHVFDITNSKRGLIRLGLPPLIGSLFFTKFLTVFHSSYPNI
CHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCE
RVEITEAGGHTLEHALEKGELDVIATVLPTSSTFEETPFVTEELYAYLPERHPLASRKQL
EEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHH
LLEELASEPFVLFTDEFSLHHVVRTACEQAGFTPRLIGESGQWDFLCMSVAQGNAVTILP
HHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEECCCCEEEEEC
RSIMQEMNIYGVKAILLKQRIPWQLGLATAKGRYLPYATREFIRYLTEEYSFDA
HHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEFREMQYFIAVARAKSFTRAAEALHVTQPTLSKTIRHLEQELGVELFDRQRRQIELTDV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHEEEHHHC
GEALMLESELLIRQLEDLPNHVFDITNSKRGLIRLGLPPLIGSLFFTKFLTVFHSSYPNI
CHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCE
RVEITEAGGHTLEHALEKGELDVIATVLPTSSTFEETPFVTEELYAYLPERHPLASRKQL
EEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHH
LLEELASEPFVLFTDEFSLHHVVRTACEQAGFTPRLIGESGQWDFLCMSVAQGNAVTILP
HHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEECCCCEEEEEC
RSIMQEMNIYGVKAILLKQRIPWQLGLATAKGRYLPYATREFIRYLTEEYSFDA
HHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]