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The map label for this gene is trmE [H]

Identifier: 229587120

GI number: 229587120

Start: 1087853

End: 1089190

Strand: Direct

Name: trmE [H]

Synonym: RAF_ORF1071

Alternate gene names: 229587120

Gene position: 1087853-1089190 (Clockwise)

Preceding gene: 229587113

Following gene: 229587121

Centisome position: 85.09

GC content: 33.26

Gene sequence:

>1338_bases
GTGGAAACGATATTTGCACAAAGCTCTGCATTCGGTAAAGCAGGCGTAGCAGTTTTTAGGATTTCTGGTCCTAAAAGTTT
AGAAGTTTTACAGCTACTTACCGGTAGAAAGGACTTTAAATCAAGATTAATGTATTATCAACAAATTACAGTCCCTGAAA
CTAAGGAGCTAATCGATAATGTTATGGTTGTTTATTTTAAGTCACCTGGTAGCTTTACCGGGGAAGACGTAGTCGAAATT
CACACGCATGGCAGTAAAGCTATATCTATAATGCTGACAAATGCATTATTAAATATAGCTGGTATTCGTTTGGCAGAAGC
AGGAGAGTTTACCAAAAGAGCTTTTTTAAATAACAAATTTGATTTAACAGCAGCAGAGGGGATAGCCGATTTAATTAATG
CCGAAACTATAATGCAGCATAAGCAAGCAATTAGACAAGCAAGCGGCAAACTTGAAGCATTATATAATAATTGGCGTAGT
CAGCTTTTAAAAATGATCTCTCTGCTTGAAGCTTATATAGATTTCCCGGATGAAGATATACCTGATACCGTCCTTAATGA
AGTTACTAATACTCATACAATTCTTGTAAATACAATATCGGAATATCTTAATGATAACAGAAAAGGAGAATTACTACGAA
GTGGTCTTAAGCTTGCAATTATCGGTCCGCCAAACGTTGGTAAGTCTAGCTTACTAAACTTCTTAATGCAGAGAGATATA
GCGATTGTCTCAAATATTGCAGGAACTACTAGAGATATAATTGAGGGACATTTAGACATTGGCGGCTATCCTATTATTTT
ACAGGATACAGCAGGTATAAGAGAAGAAAGTAGTGACATTATCGAGCAGGAAGGTATAAAACGAGCTATAAATTCAGCTA
AAACAGCAGATATTAAAATTATAATGTTTGATGCCGAAAAATTAGACTCCTCTATAAATGAAGATATTATTAATTTAATT
GATGAAAATACTATCACAATAATTAATAAAATCGATTTAATCGAAGCTAGTAAAATCTTTTCTATTGAAAATAAATATAA
ATGCTTAAGAGTTTCAGTAAAAAATAATATTGCGCTTTCAAGTATATTAAAAAATATTGAAAATATTGCCGAAAATATGG
CAGGATTTACTGAAACTCCTTATATAACAAATCAGCGTCATCGTAACTATTTACAACAAGCTCTCTCACATTTAACGGCT
TTTAGCTTAGATAATGATTTAGTTCTAGCAACTGAAGATATTAGAATGACGGCACGCTGCATCGGTGCAATTACAGGTGT
TATCAACGTAGAAGAAATACTAGGCGAGATCTTTAAAAATTTCTGCATAGGTAAGTAA

Upstream 100 bases:

>100_bases
TTGTTTTTGGGGTCATGATTTGCTTAATTAATAATAATATGATTATTTCTGTTGTTATTATATTATATAAAATAACTTAA
GCAAGAGGCATAGAAAATAA

Downstream 100 bases:

>100_bases
TATTGTATAGCTCGTTCTATGTCATCCCCGCAAGGTATTATTGCATGGACACCAAATCATCATTGCGAGCGACTGTAAGG
AGCGTGGCAACCTCATGAAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGRKDFKSRLMYYQQITVPETKELIDNVMVVYFKSPGSFTGEDVVEI
HTHGSKAISIMLTNALLNIAGIRLAEAGEFTKRAFLNNKFDLTAAEGIADLINAETIMQHKQAIRQASGKLEALYNNWRS
QLLKMISLLEAYIDFPDEDIPDTVLNEVTNTHTILVNTISEYLNDNRKGELLRSGLKLAIIGPPNVGKSSLLNFLMQRDI
AIVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKIIMFDAEKLDSSINEDIINLI
DENTITIINKIDLIEASKIFSIENKYKCLRVSVKNNIALSSILKNIENIAENMAGFTETPYITNQRHRNYLQQALSHLTA
FSLDNDLVLATEDIRMTARCIGAITGVINVEEILGEIFKNFCIGK

Sequences:

>Translated_445_residues
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGRKDFKSRLMYYQQITVPETKELIDNVMVVYFKSPGSFTGEDVVEI
HTHGSKAISIMLTNALLNIAGIRLAEAGEFTKRAFLNNKFDLTAAEGIADLINAETIMQHKQAIRQASGKLEALYNNWRS
QLLKMISLLEAYIDFPDEDIPDTVLNEVTNTHTILVNTISEYLNDNRKGELLRSGLKLAIIGPPNVGKSSLLNFLMQRDI
AIVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKIIMFDAEKLDSSINEDIINLI
DENTITIINKIDLIEASKIFSIENKYKCLRVSVKNNIALSSILKNIENIAENMAGFTETPYITNQRHRNYLQQALSHLTA
FSLDNDLVLATEDIRMTARCIGAITGVINVEEILGEIFKNFCIGK
>Mature_445_residues
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGRKDFKSRLMYYQQITVPETKELIDNVMVVYFKSPGSFTGEDVVEI
HTHGSKAISIMLTNALLNIAGIRLAEAGEFTKRAFLNNKFDLTAAEGIADLINAETIMQHKQAIRQASGKLEALYNNWRS
QLLKMISLLEAYIDFPDEDIPDTVLNEVTNTHTILVNTISEYLNDNRKGELLRSGLKLAIIGPPNVGKSSLLNFLMQRDI
AIVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKIIMFDAEKLDSSINEDIINLI
DENTITIINKIDLIEASKIFSIENKYKCLRVSVKNNIALSSILKNIENIAENMAGFTETPYITNQRHRNYLQQALSHLTA
FSLDNDLVLATEDIRMTARCIGAITGVINVEEILGEIFKNFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=459, Percent_Identity=37.6906318082789, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI193082993, Length=447, Percent_Identity=37.8076062639821, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI193082988, Length=491, Percent_Identity=35.4378818737271, Blast_Score=308, Evalue=9e-84,
Organism=Escherichia coli, GI2367268, Length=461, Percent_Identity=35.5748373101952, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=30.327868852459, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=32.3725055432373, Blast_Score=218, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=35.5555555555556, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI45550813, Length=470, Percent_Identity=38.7234042553192, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR008144
- InterPro:   IPR002917
- InterPro:   IPR022436
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49401; Mature: 49401

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGRKDFKSRLMYYQQITVPETKELIDN
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCC
VMVVYFKSPGSFTGEDVVEIHTHGSKAISIMLTNALLNIAGIRLAEAGEFTKRAFLNNKF
EEEEEECCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCEEECCCHHHHHHHCCCCC
DLTAAEGIADLINAETIMQHKQAIRQASGKLEALYNNWRSQLLKMISLLEAYIDFPDEDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDTVLNEVTNTHTILVNTISEYLNDNRKGELLRSGLKLAIIGPPNVGKSSLLNFLMQRDI
CHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
AIVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKI
HHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IMFDAEKLDSSINEDIINLIDENTITIINKIDLIEASKIFSIENKYKCLRVSVKNNIALS
EEEEHHHHCCHHHHHHHHHHCCCCEEEEEEECCEEHHHHEECCCCCEEEEEECCCCHHHH
SILKNIENIAENMAGFTETPYITNQRHRNYLQQALSHLTAFSLDNDLVLATEDIRMTARC
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
IGAITGVINVEEILGEIFKNFCIGK
HHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METIFAQSSAFGKAGVAVFRISGPKSLEVLQLLTGRKDFKSRLMYYQQITVPETKELIDN
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHCC
VMVVYFKSPGSFTGEDVVEIHTHGSKAISIMLTNALLNIAGIRLAEAGEFTKRAFLNNKF
EEEEEECCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCEEECCCHHHHHHHCCCCC
DLTAAEGIADLINAETIMQHKQAIRQASGKLEALYNNWRSQLLKMISLLEAYIDFPDEDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDTVLNEVTNTHTILVNTISEYLNDNRKGELLRSGLKLAIIGPPNVGKSSLLNFLMQRDI
CHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
AIVSNIAGTTRDIIEGHLDIGGYPIILQDTAGIREESSDIIEQEGIKRAINSAKTADIKI
HHHHHHCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
IMFDAEKLDSSINEDIINLIDENTITIINKIDLIEASKIFSIENKYKCLRVSVKNNIALS
EEEEHHHHCCHHHHHHHHHHCCCCEEEEEEECCEEHHHHEECCCCCEEEEEECCCCHHHH
SILKNIENIAENMAGFTETPYITNQRHRNYLQQALSHLTAFSLDNDLVLATEDIRMTARC
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
IGAITGVINVEEILGEIFKNFCIGK
HHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA