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The map label for this gene is ecoT [C]

Identifier: 229587019

GI number: 229587019

Start: 952155

End: 952451

Strand: Direct

Name: ecoT [C]

Synonym: RAF_ORF0922

Alternate gene names: 229587019

Gene position: 952155-952451 (Clockwise)

Preceding gene: 229587018

Following gene: 229587024

Centisome position: 74.47

GC content: 33.67

Gene sequence:

>297_bases
TTGCACCCTATCCTACACCACTTAAAAATCAAAAGTATTTATGTAATTCATTTACCTACAGAATCCAATGAAGAAAAGCT
AAAAGTAGAATTGCAAGCAACAAAAAATGCAATGCAAGATTGTAATCGTGTTTGGTTTGGTGGTAAGCTGGAAACAAAAA
CTTTAGAAGGATGGGGATATAATTATTATATAATCGATCAAGTTAACGATCATCCTGCTAGTACAATGATGGCATGCCCA
AACGTAAAAGCAACCATACAAACGGTTAGTGTATTTTTAGGCGATGAAACATTTTGA

Upstream 100 bases:

>100_bases
TAATTAAAAAAATAATCATATGCCAAATAAAATTATATTATTTTATCATGTTTTTTAAATACTGATTTATTAGCCGATAC
CGATAAGCGGTTAAAAGATA

Downstream 100 bases:

>100_bases
AGCTATAATAGCAAACTACCTATCGTAGTATATGCTCCAAAAGATGTTGAACTACATTACGTTATTTGGCATCAAAATGA
TACAATCAATTCTGCAAAGG

Product: Ecotin precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MHPILHHLKIKSIYVIHLPTESNEEKLKVELQATKNAMQDCNRVWFGGKLETKTLEGWGYNYYIIDQVNDHPASTMMACP
NVKATIQTVSVFLGDETF

Sequences:

>Translated_98_residues
MHPILHHLKIKSIYVIHLPTESNEEKLKVELQATKNAMQDCNRVWFGGKLETKTLEGWGYNYYIIDQVNDHPASTMMACP
NVKATIQTVSVFLGDETF
>Mature_98_residues
MHPILHHLKIKSIYVIHLPTESNEEKLKVELQATKNAMQDCNRVWFGGKLETKTLEGWGYNYYIIDQVNDHPASTMMACP
NVKATIQTVSVFLGDETF

Specific function: General Inhibitor Of Pancreatic Serine Proteases: Inhibits Chymotrypsin, Trypsin, Elastases, Factor X, Kallikrein As Well As A Variety Of Other Proteases. The Strength Of Inhibition Does Not Appear To Be Correlated With A Particular Protease Specificity.

COG id: COG4574

COG function: function code R; Serine protease inhibitor ecotin

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protease inhibitor I11 (ecotin) family [H]

Homologues:

Organism=Escherichia coli, GI1788538, Length=81, Percent_Identity=51.8518518518518, Blast_Score=84, Evalue=1e-18,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005658
- InterPro:   IPR023189 [H]

Pfam domain/function: PF03974 Ecotin [H]

EC number: NA

Molecular weight: Translated: 11222; Mature: 11222

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPILHHLKIKSIYVIHLPTESNEEKLKVELQATKNAMQDCNRVWFGGKLETKTLEGWGY
CCCCHHHEEEEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHEEEECCEECCEEECCCCC
NYYIIDQVNDHPASTMMACPNVKATIQTVSVFLGDETF
EEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCC
>Mature Secondary Structure
MHPILHHLKIKSIYVIHLPTESNEEKLKVELQATKNAMQDCNRVWFGGKLETKTLEGWGY
CCCCHHHEEEEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHEEEECCEECCEEECCCCC
NYYIIDQVNDHPASTMMACPNVKATIQTVSVFLGDETF
EEEEEECCCCCCCCEEEECCCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA