Click here to switch to the map view.

The map label for this gene is nrdF [H]

Identifier: 229586746

GI number: 229586746

Start: 629649

End: 630635

Strand: Direct

Name: nrdF [H]

Synonym: RAF_ORF0598

Alternate gene names: 229586746

Gene position: 629649-630635 (Clockwise)

Preceding gene: 229586745

Following gene: 229586747

Centisome position: 49.25

GC content: 36.37

Gene sequence:

>987_bases
ATGTCACTACTTGATGCAAGTCCAATTTATAAACCGTTTTCATATCCATGGGCATATGAAGCTTGGCACACTCAACAAAA
AATTCATTGGCTACCGGAAGAAGTACCGCTTGCCGACGATGTTAAAGATTGGAAATATAACTTAACTCCAGGGGAAAAAC
ATCTATTAACGCAAATATTTCGTTTTTTTACACAGGCAGATATTGAAGTGAATAATTGCTATATGAAGCATTATTCACGA
GTATTTAAACCGACTGAAATATTAATGATGTTATCAGCATTTTCTAATATGGAAACGGTACATATCGCGGCATATTCACA
TTTACTTGATACGGTAGGTATGCCGGAAGTAGAATATTCAGCATTTCTTAAATATAAGGAAATGAAAGACAAGTATGATT
ATATGCAGCAATTCGGAGTGGATGCCAAAGAAGATATAGCAACTACACTCGCAGTATTTGGAGCTTTTACTGAAGGATTA
CAGCTATTTGCTTCTTTTGCGATCATGCTTAATTTCCCTCGTTTTAATAAAATGAAAGGTATGGGACAGATTATTGCTTG
GTCAGTGCGTGATGAAACACTTCATACTAACTCAATTATTACTCTGTTTAAGACTTTTGTAAGAGAAAACCCTGAAGTTT
GGACAGAAACTTTACGTAAACGTATTTATGAAGCTTGTACGACTATTGTGCATTTTGAAGATGCCTTTATTGAACTTGCT
TTTGAAGTTGGCGGTATTGAGGGATTAACGGCATATGAAGTAAGACAATATATTCGTTATATTGCCGACCGTCGTTTAAT
GCAGCTAGGACTCAAAGATGTATATTTGGTAAATCATAACCCCCTTCCTTGGCTTGATGAAATGCTAAACGGAGTCGAAC
ATACGAACTTTTTTGAAAATAGAACAACTGAATATTCAAAAGCAGCGACTACCGGTTCATGGGAAGAAGTATTCACTGAG
ATGGACGCCACAGCGGAGGCTAAATAA

Upstream 100 bases:

>100_bases
TAGCAAAAGAAATGTTAGCGGATAATGAGCCGATCGAGAAAATAATTAAATATACTAAGCTTTCAAAAGAAGAAATAGAA
AAATTAAAAGAAGAAAAATT

Downstream 100 bases:

>100_bases
TATACTCAGATAGCACTTATGCTGAACATGTTGTGTCGAAGTATACATATTAACAATAACAAAAAATTAAAAATACCTGA
TCTTTATTTTTAAAAATTTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSLLDASPIYKPFSYPWAYEAWHTQQKIHWLPEEVPLADDVKDWKYNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYSR
VFKPTEILMMLSAFSNMETVHIAAYSHLLDTVGMPEVEYSAFLKYKEMKDKYDYMQQFGVDAKEDIATTLAVFGAFTEGL
QLFASFAIMLNFPRFNKMKGMGQIIAWSVRDETLHTNSIITLFKTFVRENPEVWTETLRKRIYEACTTIVHFEDAFIELA
FEVGGIEGLTAYEVRQYIRYIADRRLMQLGLKDVYLVNHNPLPWLDEMLNGVEHTNFFENRTTEYSKAATTGSWEEVFTE
MDATAEAK

Sequences:

>Translated_328_residues
MSLLDASPIYKPFSYPWAYEAWHTQQKIHWLPEEVPLADDVKDWKYNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYSR
VFKPTEILMMLSAFSNMETVHIAAYSHLLDTVGMPEVEYSAFLKYKEMKDKYDYMQQFGVDAKEDIATTLAVFGAFTEGL
QLFASFAIMLNFPRFNKMKGMGQIIAWSVRDETLHTNSIITLFKTFVRENPEVWTETLRKRIYEACTTIVHFEDAFIELA
FEVGGIEGLTAYEVRQYIRYIADRRLMQLGLKDVYLVNHNPLPWLDEMLNGVEHTNFFENRTTEYSKAATTGSWEEVFTE
MDATAEAK
>Mature_327_residues
SLLDASPIYKPFSYPWAYEAWHTQQKIHWLPEEVPLADDVKDWKYNLTPGEKHLLTQIFRFFTQADIEVNNCYMKHYSRV
FKPTEILMMLSAFSNMETVHIAAYSHLLDTVGMPEVEYSAFLKYKEMKDKYDYMQQFGVDAKEDIATTLAVFGAFTEGLQ
LFASFAIMLNFPRFNKMKGMGQIIAWSVRDETLHTNSIITLFKTFVRENPEVWTETLRKRIYEACTTIVHFEDAFIELAF
EVGGIEGLTAYEVRQYIRYIADRRLMQLGLKDVYLVNHNPLPWLDEMLNGVEHTNFFENRTTEYSKAATTGSWEEVFTEM
DATAEAK

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=318, Percent_Identity=29.2452830188679, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI289177074, Length=318, Percent_Identity=29.2452830188679, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4557845, Length=317, Percent_Identity=27.7602523659306, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI260064013, Length=317, Percent_Identity=27.7602523659306, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI289177076, Length=297, Percent_Identity=29.2929292929293, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551968, Length=283, Percent_Identity=27.5618374558304, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322434, Length=303, Percent_Identity=26.0726072607261, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18079279, Length=317, Percent_Identity=28.391167192429, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38328; Mature: 38197

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLDASPIYKPFSYPWAYEAWHTQQKIHWLPEEVPLADDVKDWKYNLTPGEKHLLTQIF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
RFFTQADIEVNNCYMKHYSRVFKPTEILMMLSAFSNMETVHIAAYSHLLDTVGMPEVEYS
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHH
AFLKYKEMKDKYDYMQQFGVDAKEDIATTLAVFGAFTEGLQLFASFAIMLNFPRFNKMKG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIIAWSVRDETLHTNSIITLFKTFVRENPEVWTETLRKRIYEACTTIVHFEDAFIELA
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEVGGIEGLTAYEVRQYIRYIADRRLMQLGLKDVYLVNHNPLPWLDEMLNGVEHTNFFEN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
RTTEYSKAATTGSWEEVFTEMDATAEAK
CCCHHHHHCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLLDASPIYKPFSYPWAYEAWHTQQKIHWLPEEVPLADDVKDWKYNLTPGEKHLLTQIF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
RFFTQADIEVNNCYMKHYSRVFKPTEILMMLSAFSNMETVHIAAYSHLLDTVGMPEVEYS
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHH
AFLKYKEMKDKYDYMQQFGVDAKEDIATTLAVFGAFTEGLQLFASFAIMLNFPRFNKMKG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIIAWSVRDETLHTNSIITLFKTFVRENPEVWTETLRKRIYEACTTIVHFEDAFIELA
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEVGGIEGLTAYEVRQYIRYIADRRLMQLGLKDVYLVNHNPLPWLDEMLNGVEHTNFFEN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
RTTEYSKAATTGSWEEVFTEMDATAEAK
CCCHHHHHCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]