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The map label for this gene is uvrD [H]

Identifier: 229586731

GI number: 229586731

Start: 607809

End: 609770

Strand: Direct

Name: uvrD [H]

Synonym: RAF_ORF0580

Alternate gene names: 229586731

Gene position: 607809-609770 (Clockwise)

Preceding gene: 229586730

Following gene: 229586732

Centisome position: 47.54

GC content: 33.23

Gene sequence:

>1962_bases
ATGCAAAATCAAGATTTCATACATACATTAAATCCAGAACAGCAAAAAGCAGTTCTTCACACTGAAGGACCGCTTTTATT
GCTTGCAGGTGCAGGGACAGGTAAAACAAAGGTACTTACCTCAAGAATAGCTAATATTATTCACCAAAATTTAGCCTCAC
CTCAAAATATTCTAGCTGTTACTTTTACTAATAAAGCTGCTAAAGAAATGGCTGAAAGAGTTAATAGTCTAATTAATTGC
TATGGTCTTAATATAGGTACTTTCCACTCAATGGCAGCTAGGATATTACGTGACCAGATTGAGCATTTAAATCTTGGTTT
AAATAATAGATTTACTATTATTAGTCATGATGATCAATTAAAACTAGTGAAAGATATAGTAAAGCTAAAAGATATTGATA
CTAAAAAATATGCTCCTAAATTAATACATATCATAATTTCTAGATGGAAAGATCAAGGATTATTACCAACTAAACTATCA
GTATCAGATACTAATTTACCGTTGCAGAGGGTTGCAAAACTTGTATACGAAGAATATCAGAAAAATTTACTTATTTCTAA
TGTTTTAGATTTTGGAGATTTGCTACTTTATAATAATGAGCTTTTTATTAAAAATTCTGAAATACTAAGATATTACCAAG
AAAAATATCGATATATTTTAATTGATGAATATCAAGACACTAACGTTGTCCAGTATTTATGGGCAAGAATGCTCGCAAGT
TTATATAAAAATATTTGCTGTGTAGGGGATGATGATCAATCTATTTATGGTTGGCGAGGAGCAGAAGTCGGGAATATATT
ACGTTTTGAAAAAGATTTTGCAGGTGCAACAATTATTAAATTAGAGCAGAATTATAGATCAACTTTGCCAATACTTGCTG
CTGCTTCCAATGTTATCAATAATAATAAAAACCGTCACGGTAAAACGTTATGGACGGATAGGGAAAACGGTGAAAAGATA
AAAATTATCTCTTGTTGGAGTGATAAAGAAGAAGCAAGATATATAGCCAGTGAAATAGATAATTTAGTGCGAGAAAACCG
GTATAATGCCGGCAATATTGCAATATTAGTACGAGCCGTATTTCAAACAAGAAGTTTTGAGGAAGCGTTTATAAATAGTG
CTATGCCTTATAAAATTATAGGTGGTTTGCGGTTCTATGAACGTATGGAAATAAGAGATGTACTTGCTTATATCCGTATA
TCTTTGAATCAAAATGACAATCTAGCACTTGAGCGTATTATAAATGTGCCGAAAAGGGCAATAGGTGCAGCTAGCTTAAA
TAAAATTAGAGGCTATGCACTTGAGCGAAATATTTCTAATTTCGCAGCCATTAAAGAAATGCTAGAAATAGGTGAGATTA
AAGCAAAATCATATGAAACTCTAAAAGATTTGGTTACCAAAATCGATAATTGGTACGAAAGATTTAGTATAGATGCTCCA
ATAAACGTAGTTAAAGCAATACTTGATGATTCCGGTTATCTTGAAATGCTTCAAGAAGAAAAAACCGAGGAAGCATTCGG
TAGAATCGAAAATATTAACGAAATGCTTAGAGCTATTGCTGAATTTAACGATGTTCATGATTTTATCGAACATTCAAGCT
TAGTTATGGAAAATGAAGTGCTTGAAACCAATTACGGTGGCTCTGTTACTATAATGACCCTGCACGCAGCTAAAGGGCTT
GAGTTTGACGTGGTATTTTTACCGGGCTGGGAAGAGGGGGTATTTCCGTCTCAAAGATCTCTAGACGAAGACGGCGAAAA
AGGCTTAGAAGAAGAACGCCGCATTGCTTATGTCGGTATTACGAGAGCCAAAAAAGACCTCTACATTACTCATGCTGAAA
GCAGAAAAATATTTTATGAAATAGTCCGTTCTTACCCTTCAAGGTTCATAACTGAAATCCCTGATGAAATTACGATTAGG
ACCTCTTCTATGAAAAAATACAATTCTTTTTATAAGTTTTAA

Upstream 100 bases:

>100_bases
AAAATCTAAGAAGAATAAGGTGATATTTTAATGCTTTAATGTCATTCCCGCTTTTGCGGGAATGACATAGTAGAGCCATA
GTACAACATCCTTAAAATAA

Downstream 100 bases:

>100_bases
TTAATTTTCATTCATAAATTTTAAATTGGTTTGCCATAGATTTAAGTGAGTCTCAAGTAGAGCAGATGCACGACGAAAAA
CGTAATGGCTTAAGCGAAGA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MQNQDFIHTLNPEQQKAVLHTEGPLLLLAGAGTGKTKVLTSRIANIIHQNLASPQNILAVTFTNKAAKEMAERVNSLINC
YGLNIGTFHSMAARILRDQIEHLNLGLNNRFTIISHDDQLKLVKDIVKLKDIDTKKYAPKLIHIIISRWKDQGLLPTKLS
VSDTNLPLQRVAKLVYEEYQKNLLISNVLDFGDLLLYNNELFIKNSEILRYYQEKYRYILIDEYQDTNVVQYLWARMLAS
LYKNICCVGDDDQSIYGWRGAEVGNILRFEKDFAGATIIKLEQNYRSTLPILAAASNVINNNKNRHGKTLWTDRENGEKI
KIISCWSDKEEARYIASEIDNLVRENRYNAGNIAILVRAVFQTRSFEEAFINSAMPYKIIGGLRFYERMEIRDVLAYIRI
SLNQNDNLALERIINVPKRAIGAASLNKIRGYALERNISNFAAIKEMLEIGEIKAKSYETLKDLVTKIDNWYERFSIDAP
INVVKAILDDSGYLEMLQEEKTEEAFGRIENINEMLRAIAEFNDVHDFIEHSSLVMENEVLETNYGGSVTIMTLHAAKGL
EFDVVFLPGWEEGVFPSQRSLDEDGEKGLEEERRIAYVGITRAKKDLYITHAESRKIFYEIVRSYPSRFITEIPDEITIR
TSSMKKYNSFYKF

Sequences:

>Translated_653_residues
MQNQDFIHTLNPEQQKAVLHTEGPLLLLAGAGTGKTKVLTSRIANIIHQNLASPQNILAVTFTNKAAKEMAERVNSLINC
YGLNIGTFHSMAARILRDQIEHLNLGLNNRFTIISHDDQLKLVKDIVKLKDIDTKKYAPKLIHIIISRWKDQGLLPTKLS
VSDTNLPLQRVAKLVYEEYQKNLLISNVLDFGDLLLYNNELFIKNSEILRYYQEKYRYILIDEYQDTNVVQYLWARMLAS
LYKNICCVGDDDQSIYGWRGAEVGNILRFEKDFAGATIIKLEQNYRSTLPILAAASNVINNNKNRHGKTLWTDRENGEKI
KIISCWSDKEEARYIASEIDNLVRENRYNAGNIAILVRAVFQTRSFEEAFINSAMPYKIIGGLRFYERMEIRDVLAYIRI
SLNQNDNLALERIINVPKRAIGAASLNKIRGYALERNISNFAAIKEMLEIGEIKAKSYETLKDLVTKIDNWYERFSIDAP
INVVKAILDDSGYLEMLQEEKTEEAFGRIENINEMLRAIAEFNDVHDFIEHSSLVMENEVLETNYGGSVTIMTLHAAKGL
EFDVVFLPGWEEGVFPSQRSLDEDGEKGLEEERRIAYVGITRAKKDLYITHAESRKIFYEIVRSYPSRFITEIPDEITIR
TSSMKKYNSFYKF
>Mature_653_residues
MQNQDFIHTLNPEQQKAVLHTEGPLLLLAGAGTGKTKVLTSRIANIIHQNLASPQNILAVTFTNKAAKEMAERVNSLINC
YGLNIGTFHSMAARILRDQIEHLNLGLNNRFTIISHDDQLKLVKDIVKLKDIDTKKYAPKLIHIIISRWKDQGLLPTKLS
VSDTNLPLQRVAKLVYEEYQKNLLISNVLDFGDLLLYNNELFIKNSEILRYYQEKYRYILIDEYQDTNVVQYLWARMLAS
LYKNICCVGDDDQSIYGWRGAEVGNILRFEKDFAGATIIKLEQNYRSTLPILAAASNVINNNKNRHGKTLWTDRENGEKI
KIISCWSDKEEARYIASEIDNLVRENRYNAGNIAILVRAVFQTRSFEEAFINSAMPYKIIGGLRFYERMEIRDVLAYIRI
SLNQNDNLALERIINVPKRAIGAASLNKIRGYALERNISNFAAIKEMLEIGEIKAKSYETLKDLVTKIDNWYERFSIDAP
INVVKAILDDSGYLEMLQEEKTEEAFGRIENINEMLRAIAEFNDVHDFIEHSSLVMENEVLETNYGGSVTIMTLHAAKGL
EFDVVFLPGWEEGVFPSQRSLDEDGEKGLEEERRIAYVGITRAKKDLYITHAESRKIFYEIVRSYPSRFITEIPDEITIR
TSSMKKYNSFYKF

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=644, Percent_Identity=41.4596273291925, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI48994965, Length=648, Percent_Identity=38.4259259259259, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=88, Percent_Identity=43.1818181818182, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322369, Length=514, Percent_Identity=31.5175097276265, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=685, Percent_Identity=23.2116788321168, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75096; Mature: 75096

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQDFIHTLNPEQQKAVLHTEGPLLLLAGAGTGKTKVLTSRIANIIHQNLASPQNILAV
CCCCCCEECCCCCHHCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFTNKAAKEMAERVNSLINCYGLNIGTFHSMAARILRDQIEHLNLGLNNRFTIISHDDQL
EECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
KLVKDIVKLKDIDTKKYAPKLIHIIISRWKDQGLLPTKLSVSDTNLPLQRVAKLVYEEYQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHH
KNLLISNVLDFGDLLLYNNELFIKNSEILRYYQEKYRYILIDEYQDTNVVQYLWARMLAS
HHHHHHHHHHHCCEEEECCEEEEECHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
LYKNICCVGDDDQSIYGWRGAEVGNILRFEKDFAGATIIKLEQNYRSTLPILAAASNVIN
HHHCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCHHCCHHHHHHHHHHC
NNKNRHGKTLWTDRENGEKIKIISCWSDKEEARYIASEIDNLVRENRYNAGNIAILVRAV
CCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
FQTRSFEEAFINSAMPYKIIGGLRFYERMEIRDVLAYIRISLNQNDNLALERIINVPKRA
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCHHH
IGAASLNKIRGYALERNISNFAAIKEMLEIGEIKAKSYETLKDLVTKIDNWYERFSIDAP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCC
INVVKAILDDSGYLEMLQEEKTEEAFGRIENINEMLRAIAEFNDVHDFIEHSSLVMENEV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LETNYGGSVTIMTLHAAKGLEFDVVFLPGWEEGVFPSQRSLDEDGEKGLEEERRIAYVGI
HCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHCCCHHHCEEEEEEE
TRAKKDLYITHAESRKIFYEIVRSYPSRFITEIPDEITIRTSSMKKYNSFYKF
EECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MQNQDFIHTLNPEQQKAVLHTEGPLLLLAGAGTGKTKVLTSRIANIIHQNLASPQNILAV
CCCCCCEECCCCCHHCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFTNKAAKEMAERVNSLINCYGLNIGTFHSMAARILRDQIEHLNLGLNNRFTIISHDDQL
EECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
KLVKDIVKLKDIDTKKYAPKLIHIIISRWKDQGLLPTKLSVSDTNLPLQRVAKLVYEEYQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHH
KNLLISNVLDFGDLLLYNNELFIKNSEILRYYQEKYRYILIDEYQDTNVVQYLWARMLAS
HHHHHHHHHHHCCEEEECCEEEEECHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
LYKNICCVGDDDQSIYGWRGAEVGNILRFEKDFAGATIIKLEQNYRSTLPILAAASNVIN
HHHCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCHHCCHHHHHHHHHHC
NNKNRHGKTLWTDRENGEKIKIISCWSDKEEARYIASEIDNLVRENRYNAGNIAILVRAV
CCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
FQTRSFEEAFINSAMPYKIIGGLRFYERMEIRDVLAYIRISLNQNDNLALERIINVPKRA
HHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCHHH
IGAASLNKIRGYALERNISNFAAIKEMLEIGEIKAKSYETLKDLVTKIDNWYERFSIDAP
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCC
INVVKAILDDSGYLEMLQEEKTEEAFGRIENINEMLRAIAEFNDVHDFIEHSSLVMENEV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LETNYGGSVTIMTLHAAKGLEFDVVFLPGWEEGVFPSQRSLDEDGEKGLEEERRIAYVGI
HCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHCCCHHHCEEEEEEE
TRAKKDLYITHAESRKIFYEIVRSYPSRFITEIPDEITIRTSSMKKYNSFYKF
EECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]