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The map label for this gene is smpB

Identifier: 229586708

GI number: 229586708

Start: 584653

End: 585111

Strand: Direct

Name: smpB

Synonym: RAF_ORF0555

Alternate gene names: 229586708

Gene position: 584653-585111 (Clockwise)

Preceding gene: 229586707

Following gene: 229586709

Centisome position: 45.73

GC content: 32.03

Gene sequence:

>459_bases
ATGACTGAATATAAGAAAGTTATTGCACAAAATAAGAAAGCCCTTTTTAATTATTTTATTGCAGAAAGATTAGAAGCGGG
TATTGTATTAAAGGGTAGTGAAGTACGGTCTCTGCGTCAGGGCAAAGCTTCTATAGAAGAAAGTCATGCGGCAGATACCG
GACATGAGGTATTTTTGTATAACTGCCATATTGCAGAATATGAAAAAGCGAATAGATTTAATCATGCTACTCGTAGACCG
CGTAAATTATTGTTGCATACTAAAGAGATAAAGAAAATTATCGGTAGAATTAGAATAAAAGGCTATACGCTAGTAGCTCT
TTCTATGTATTTTAATAAAAAAAATAAAGTGAAAGTTGAACTTGGTATTGCTAAAGGTAAGAAATTACATGATAAAAGAG
AGTCAATTAAAGAAAAAGACTGGAAAAGAGATCAGAGCAGATTAATAAGACAGAAGTAA

Upstream 100 bases:

>100_bases
TAGGTTTATGTGAAAATGAAATTAGACCCCCATTAACTGAAGCAAGCGATAGTGCCAAAAAACAAATTGAAAACATCATA
ACATCACTATCTATAAAAAT

Downstream 100 bases:

>100_bases
TGGACTTTGCTACACCGGATGACAAGCCGGCTTTGTTACATAGCTCGTTTTATGTCATTCCCGCTTTCGCGGGAATGACA
TATAGAATTACTTAGACTTT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MTEYKKVIAQNKKALFNYFIAERLEAGIVLKGSEVRSLRQGKASIEESHAADTGHEVFLYNCHIAEYEKANRFNHATRRP
RKLLLHTKEIKKIIGRIRIKGYTLVALSMYFNKKNKVKVELGIAKGKKLHDKRESIKEKDWKRDQSRLIRQK

Sequences:

>Translated_152_residues
MTEYKKVIAQNKKALFNYFIAERLEAGIVLKGSEVRSLRQGKASIEESHAADTGHEVFLYNCHIAEYEKANRFNHATRRP
RKLLLHTKEIKKIIGRIRIKGYTLVALSMYFNKKNKVKVELGIAKGKKLHDKRESIKEKDWKRDQSRLIRQK
>Mature_151_residues
TEYKKVIAQNKKALFNYFIAERLEAGIVLKGSEVRSLRQGKASIEESHAADTGHEVFLYNCHIAEYEKANRFNHATRRPR
KLLLHTKEIKKIIGRIRIKGYTLVALSMYFNKKNKVKVELGIAKGKKLHDKRESIKEKDWKRDQSRLIRQK

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=147, Percent_Identity=44.8979591836735, Blast_Score=120, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_RICAE (C3PNF6)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845209.1
- GeneID:   7814972
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0555
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17822; Mature: 17691

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYKKVIAQNKKALFNYFIAERLEAGIVLKGSEVRSLRQGKASIEESHAADTGHEVFLY
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NCHIAEYEKANRFNHATRRPRKLLLHTKEIKKIIGRIRIKGYTLVALSMYFNKKNKVKVE
ECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHCCCCCEEEE
LGIAKGKKLHDKRESIKEKDWKRDQSRLIRQK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEYKKVIAQNKKALFNYFIAERLEAGIVLKGSEVRSLRQGKASIEESHAADTGHEVFLY
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NCHIAEYEKANRFNHATRRPRKLLLHTKEIKKIIGRIRIKGYTLVALSMYFNKKNKVKVE
ECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEHHCCCCCEEEE
LGIAKGKKLHDKRESIKEKDWKRDQSRLIRQK
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA