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The map label for this gene is ruvB [H]

Identifier: 229586658

GI number: 229586658

Start: 524111

End: 525139

Strand: Direct

Name: ruvB [H]

Synonym: RAF_ORF0495

Alternate gene names: 229586658

Gene position: 524111-525139 (Clockwise)

Preceding gene: 229586657

Following gene: 229586659

Centisome position: 40.99

GC content: 37.71

Gene sequence:

>1029_bases
ATGACTAATATATTATCACCTGAAAAAAGTGAAAATGATCAAGAATTGCCGATAAGGCCGTCATATTTGCAAGAATTTGT
CGGTCAACAGCAGATTAAAGAAAATCTTTCGGTATTTATTAAGGCTGCAAAATCTCGAAATGAGCATCTTGATCATACTT
TATTTTACGGTCCGCCCGGGCTTGGTAAAACTACGCTTGCTAAAATTATCTCTAACGAAATCGGCGGTAATTTTAAATCC
ACCTCCGGTCCTGCTATACTTAAGGTCGCTGATCTTGCTGCTATTCTTACCAATCTTGAAAAGAACGATGTATTATTTAT
TGATGAAATCCACCGCTTAAATACTGCGATTGAAGAGGTTTTATATCCTGCTATGGAAGATTTTGAGCTTGATATAATTA
TCGGTGAAGGATCTGCCGCAAGGTCAGTGAAAATAACCTTGCCGAAATTTACCTTAATCGGTGCTACCACACGCCTCGGG
CTGCTTAGTAATCCTTTGCGTGATAGGTTCGGTATCCCTATGCGGCTAAATTTTTATAATACCGAGGAATTGAAGAAAGT
TCTAAATAGAGCAAGTAAACTACTTGATATTGATTTGACCGATTCGGGTTCTGAAGAAATAGCTAAAAGAAGTAGAGGAA
CGCCAAGAATTGCATTAAGATTGCTTCGTCGTATAAGAGATTTTGCAGCAGTTGACGGTAAATCAAGAGTAGATAAGGAA
ATCTCTGATTTTGGGTTAAATCGTTTGGAAGTTGATTGCATAGGACTTGATAGCAATGATTATCGTTATCTAAAATTCAT
AGCGGATAATTATAACGGCGGTCCTGTCGGAATTGAAACGATTGCAGCTGCCTTATCTGAACAACGTGATGCGCTTGAAG
AAACCATAGAGCCTTATCTTATACAGATAGGTTTACTACAAAGAACTCCTAGAGGTAGGGTAATCACGATTGCCGCTTTT
GAGCATTTGAAAATGCCTGTACCGAATCAATCACATCATCAATTTAATATTTTTAACGAGAATGAATAA

Upstream 100 bases:

>100_bases
CACTAGTTTCTATGAAGAGGGCTAATATAAAAGTCTAAAATAATTTATAGCATAAAACTATTTTTTGGCTATTATTAGTC
ATTATATTCGATTTAAAATA

Downstream 100 bases:

>100_bases
CAATAATTTAAATGATAACTACACAGCCGCAGATGTTATAAAAAGGCTGATAAAAGATCATGTTAAACCTTACGCAAATA
AAATATATTTTTCGATATTT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTNILSPEKSENDQELPIRPSYLQEFVGQQQIKENLSVFIKAAKSRNEHLDHTLFYGPPGLGKTTLAKIISNEIGGNFKS
TSGPAILKVADLAAILTNLEKNDVLFIDEIHRLNTAIEEVLYPAMEDFELDIIIGEGSAARSVKITLPKFTLIGATTRLG
LLSNPLRDRFGIPMRLNFYNTEELKKVLNRASKLLDIDLTDSGSEEIAKRSRGTPRIALRLLRRIRDFAAVDGKSRVDKE
ISDFGLNRLEVDCIGLDSNDYRYLKFIADNYNGGPVGIETIAAALSEQRDALEETIEPYLIQIGLLQRTPRGRVITIAAF
EHLKMPVPNQSHHQFNIFNENE

Sequences:

>Translated_342_residues
MTNILSPEKSENDQELPIRPSYLQEFVGQQQIKENLSVFIKAAKSRNEHLDHTLFYGPPGLGKTTLAKIISNEIGGNFKS
TSGPAILKVADLAAILTNLEKNDVLFIDEIHRLNTAIEEVLYPAMEDFELDIIIGEGSAARSVKITLPKFTLIGATTRLG
LLSNPLRDRFGIPMRLNFYNTEELKKVLNRASKLLDIDLTDSGSEEIAKRSRGTPRIALRLLRRIRDFAAVDGKSRVDKE
ISDFGLNRLEVDCIGLDSNDYRYLKFIADNYNGGPVGIETIAAALSEQRDALEETIEPYLIQIGLLQRTPRGRVITIAAF
EHLKMPVPNQSHHQFNIFNENE
>Mature_341_residues
TNILSPEKSENDQELPIRPSYLQEFVGQQQIKENLSVFIKAAKSRNEHLDHTLFYGPPGLGKTTLAKIISNEIGGNFKST
SGPAILKVADLAAILTNLEKNDVLFIDEIHRLNTAIEEVLYPAMEDFELDIIIGEGSAARSVKITLPKFTLIGATTRLGL
LSNPLRDRFGIPMRLNFYNTEELKKVLNRASKLLDIDLTDSGSEEIAKRSRGTPRIALRLLRRIRDFAAVDGKSRVDKEI
SDFGLNRLEVDCIGLDSNDYRYLKFIADNYNGGPVGIETIAAALSEQRDALEETIEPYLIQIGLLQRTPRGRVITIAAFE
HLKMPVPNQSHHQFNIFNENE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=311, Percent_Identity=55.6270096463022, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1787119, Length=229, Percent_Identity=28.82096069869, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 38343; Mature: 38212

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNILSPEKSENDQELPIRPSYLQEFVGQQQIKENLSVFIKAAKSRNEHLDHTLFYGPPG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
LGKTTLAKIISNEIGGNFKSTSGPAILKVADLAAILTNLEKNDVLFIDEIHRLNTAIEEV
CCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
LYPAMEDFELDIIIGEGSAARSVKITLPKFTLIGATTRLGLLSNPLRDRFGIPMRLNFYN
HHHCCCCCEEEEEEECCCCCCEEEEEECCEEEEECHHHHHHHCCCHHHHCCCCEEEECCC
TEELKKVLNRASKLLDIDLTDSGSEEIAKRSRGTPRIALRLLRRIRDFAAVDGKSRVDKE
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ISDFGLNRLEVDCIGLDSNDYRYLKFIADNYNGGPVGIETIAAALSEQRDALEETIEPYL
HHHCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
IQIGLLQRTPRGRVITIAAFEHLKMPVPNQSHHQFNIFNENE
EEHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
TNILSPEKSENDQELPIRPSYLQEFVGQQQIKENLSVFIKAAKSRNEHLDHTLFYGPPG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
LGKTTLAKIISNEIGGNFKSTSGPAILKVADLAAILTNLEKNDVLFIDEIHRLNTAIEEV
CCHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
LYPAMEDFELDIIIGEGSAARSVKITLPKFTLIGATTRLGLLSNPLRDRFGIPMRLNFYN
HHHCCCCCEEEEEEECCCCCCEEEEEECCEEEEECHHHHHHHCCCHHHHCCCCEEEECCC
TEELKKVLNRASKLLDIDLTDSGSEEIAKRSRGTPRIALRLLRRIRDFAAVDGKSRVDKE
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ISDFGLNRLEVDCIGLDSNDYRYLKFIADNYNGGPVGIETIAAALSEQRDALEETIEPYL
HHHCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
IQIGLLQRTPRGRVITIAAFEHLKMPVPNQSHHQFNIFNENE
EEHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA