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The map label for this gene is ruvA

Identifier: 229586656

GI number: 229586656

Start: 522502

End: 523113

Strand: Direct

Name: ruvA

Synonym: RAF_ORF0493

Alternate gene names: 229586656

Gene position: 522502-523113 (Clockwise)

Preceding gene: 229586655

Following gene: 229586657

Centisome position: 40.87

GC content: 33.66

Gene sequence:

>612_bases
ATGATCGGTCAATTAAGTGGTAAAGTTGATGCCCAAGGCGATGATTACGTTATAATTGACGTAAACGGGGTAGGGTACTT
AGTCTATGCTTCAGGTAAAACTTTAGGTAAACTTGCTGAAGGGGAGTTTTATAAACTATTTATCGAAACCCATGTTAGAG
AGGAACATATTCATCTTTACGGTTTTTTGAATCTAGAAGAGAAGATTTTTTTTAATTTACTACAATCAGTAAACGGTATA
GGTACAAGAATGGCTTTATTTATCTTATCAAGCCTTACGCCTTCAGATATTCAAATTGCTGTAAATAATGAAGATAAAAA
TATATTTAAAGCAATATCCGGAGTAGGGGCTAAACTTGCTGAACGTATAGTGCTAGAGCTGAAAGGTAAAGTAGCGAAAA
TATCAAGCGGTTCAGCTATTATTAAAGAGAGCTTAAATATTAAGCATATCACACCCGTTGCAAGTAATGAAGTAATAAAA
GCTCTAGTAAATCTAGGATTTTCTAGATTTGAAGCTCAAAACGCTGTTCAAGGCATCATTACCCAAAATCCCGAAATCTC
TATTGATGAATTAATCAAAACAGCCCTGAAAAATCGAAATTCTAATTTTTAG

Upstream 100 bases:

>100_bases
ATGATGGATAGACCGTTTGTTATTCATTACATCTTAGCAGCAATTATTACCGCTTTACCGACACTAATTTTAATATATAG
TAAAAAGAAAAAGTAAATAT

Downstream 100 bases:

>100_bases
TTGATAAATTAGCAGTTATTTTATATTAATTTTAATTACTAAAATTAATAGGTACAATATATGTCCAAGTTAATAGAATA
TTTAGGAAAAATACAGGCTG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIGQLSGKVDAQGDDYVIIDVNGVGYLVYASGKTLGKLAEGEFYKLFIETHVREEHIHLYGFLNLEEKIFFNLLQSVNGI
GTRMALFILSSLTPSDIQIAVNNEDKNIFKAISGVGAKLAERIVLELKGKVAKISSGSAIIKESLNIKHITPVASNEVIK
ALVNLGFSRFEAQNAVQGIITQNPEISIDELIKTALKNRNSNF

Sequences:

>Translated_203_residues
MIGQLSGKVDAQGDDYVIIDVNGVGYLVYASGKTLGKLAEGEFYKLFIETHVREEHIHLYGFLNLEEKIFFNLLQSVNGI
GTRMALFILSSLTPSDIQIAVNNEDKNIFKAISGVGAKLAERIVLELKGKVAKISSGSAIIKESLNIKHITPVASNEVIK
ALVNLGFSRFEAQNAVQGIITQNPEISIDELIKTALKNRNSNF
>Mature_203_residues
MIGQLSGKVDAQGDDYVIIDVNGVGYLVYASGKTLGKLAEGEFYKLFIETHVREEHIHLYGFLNLEEKIFFNLLQSVNGI
GTRMALFILSSLTPSDIQIAVNNEDKNIFKAISGVGAKLAERIVLELKGKVAKISSGSAIIKESLNIKHITPVASNEVIK
ALVNLGFSRFEAQNAVQGIITQNPEISIDELIKTALKNRNSNF

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=202, Percent_Identity=32.6732673267327, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_RICAE (C3PNA4)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845157.1
- GeneID:   7815559
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0493
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22220; Mature: 22220

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGQLSGKVDAQGDDYVIIDVNGVGYLVYASGKTLGKLAEGEFYKLFIETHVREEHIHLY
CCCCCCCEEECCCCCEEEEEECCCEEEEEECCCCHHHHCCCCEEEEEEEHHCCCCEEEEE
GFLNLEEKIFFNLLQSVNGIGTRMALFILSSLTPSDIQIAVNNEDKNIFKAISGVGAKLA
EEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
ERIVLELKGKVAKISSGSAIIKESLNIKHITPVASNEVIKALVNLGFSRFEAQNAVQGII
HHHHHHHCCCEEEECCCCHHHHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
TQNPEISIDELIKTALKNRNSNF
CCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIGQLSGKVDAQGDDYVIIDVNGVGYLVYASGKTLGKLAEGEFYKLFIETHVREEHIHLY
CCCCCCCEEECCCCCEEEEEECCCEEEEEECCCCHHHHCCCCEEEEEEEHHCCCCEEEEE
GFLNLEEKIFFNLLQSVNGIGTRMALFILSSLTPSDIQIAVNNEDKNIFKAISGVGAKLA
EEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
ERIVLELKGKVAKISSGSAIIKESLNIKHITPVASNEVIKALVNLGFSRFEAQNAVQGII
HHHHHHHCCCEEEECCCCHHHHHCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
TQNPEISIDELIKTALKNRNSNF
CCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA