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The map label for this gene is proS [H]

Identifier: 229586653

GI number: 229586653

Start: 519639

End: 520919

Strand: Direct

Name: proS [H]

Synonym: RAF_ORF0490

Alternate gene names: 229586653

Gene position: 519639-520919 (Clockwise)

Preceding gene: 229586652

Following gene: 229586654

Centisome position: 40.64

GC content: 33.96

Gene sequence:

>1281_bases
ATGTTATTATCAAAATATTTTTTACCTGTATTAAAGGAAGAGCCAAGTGAAGCTCAAGTAACTTCGCATAAATTAATGCT
TAGAAGCGGAATGATTAGGCAGCAAGCAGCAGGGATTTATACTTGGCTTCCGCTTGGCTTAAAAGTATTGAAGAATATCG
AGAATATAGTACGCTTAAATATGAATAAAGCGGGAGCTTTGGAAGTTCTAATGCCTTGCATTCAGCCGGCACATTTATGG
ATGGAATCGGGACGTTTTGATAATTACGGTAAAGAAATGCTGAAATTTCAAGATCGCCACGATAATACTTTACTATTCGG
TCCTACTAACGAAGATATGATTACGGATATTTTTCGTCATAATATTAAGTCATATAAGGATTTGCCGAAAAATCTTTATC
ATATCCAATGGAAATTTCGTGATGAGATTAGACCTCGTTTTGGTGTTATGAGAGGACGAGAGTTTTTAATGAAAGATGCT
TATTCTTTTGATATAAATGAAGAAAATGCCGTTAAAACCTATAATCAAATGTATAAGGCTTATATCAATGCTTTCAGGGA
TTTAGGCGTGTTTGTTATCCCTGTTATTGCCGATAACGGTCCGATCGGTGGCAATTTAAGCCATGAGTTTCATATAATCG
CTGAAACTGGTGAAAGTACTATTTATTACGATAAAAAATTTAAGACATTAAAAGATAATCCTGATATCGATGTAGAGGAA
ATTAAAAGTTGGTACGCAGCAGCTGAAGAAAAATATGAAGTAAATAAATTACCTATATCTGAGCAAGAAATAACCAGCAG
TAAAGGAATAGAAGTCGGGCATATTTTTTATATAGGCTCAAAATATTCGGTTAATATGAATGCACTTATTAATGATGAGT
ATGGTAAACTAACTCCTATAGAAATGAGTTCTTACGGCATAGGCATTTCAAGGTTAGTTGCTGCTATCATAGAAGCAAAT
TGCGATGAGAAAGGTATCATATGGCCTTCTAGCGTTGCTCCTTTCAAAGTTTCTCTAATTAATTTAAATATTCATGATAG
TAAATGCGTAGAGCTTGCGGAAATGGCTTATAAAGAGCTATCAGATAAAAATATAGAAGTGTTATATGATGATACCGAAG
CACGTCCAGGCAGTAAATTTGCAACGCATGATCTTATAGGCTCACCTCATCAAATTATAATTGGTCCTAAAAAAGCCGCA
AATAATATTGTTGAATTAAAAGATCGTAAAAGTGGTGTTATAGAAGATATTGAGGTAGGTAGTCTAATGAGTGTATTGTA
A

Upstream 100 bases:

>100_bases
TGCGGTATCCAGAAAAAAATTTAAAAAAACTGGATCCCGTGGTCAAGCCACGGGATAACCAAATAAATGATTATAAATAT
AGCAAGAAAGTAAATAAAAT

Downstream 100 bases:

>100_bases
AATAAAGAGATATACGTCATTACGAGAAAATTGCATAGCAATTGATGAGGCAATCTTAGGAGTTTGTTATTATTTCGTGA
GATTGCCACGCAGTCTACGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLLSKYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVRLNMNKAGALEVLMPCIQPAHLW
MESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDA
YSFDINEENAVKTYNQMYKAYINAFRDLGVFVIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFKTLKDNPDIDVEE
IKSWYAAAEEKYEVNKLPISEQEITSSKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPIEMSSYGIGISRLVAAIIEAN
CDEKGIIWPSSVAPFKVSLINLNIHDSKCVELAEMAYKELSDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAA
NNIVELKDRKSGVIEDIEVGSLMSVL

Sequences:

>Translated_426_residues
MLLSKYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVRLNMNKAGALEVLMPCIQPAHLW
MESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDA
YSFDINEENAVKTYNQMYKAYINAFRDLGVFVIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFKTLKDNPDIDVEE
IKSWYAAAEEKYEVNKLPISEQEITSSKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPIEMSSYGIGISRLVAAIIEAN
CDEKGIIWPSSVAPFKVSLINLNIHDSKCVELAEMAYKELSDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAA
NNIVELKDRKSGVIEDIEVGSLMSVL
>Mature_426_residues
MLLSKYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVRLNMNKAGALEVLMPCIQPAHLW
MESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDA
YSFDINEENAVKTYNQMYKAYINAFRDLGVFVIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFKTLKDNPDIDVEE
IKSWYAAAEEKYEVNKLPISEQEITSSKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPIEMSSYGIGISRLVAAIIEAN
CDEKGIIWPSSVAPFKVSLINLNIHDSKCVELAEMAYKELSDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAA
NNIVELKDRKSGVIEDIEVGSLMSVL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=395, Percent_Identity=35.4430379746835, Blast_Score=249, Evalue=4e-66,
Organism=Escherichia coli, GI1786392, Length=223, Percent_Identity=56.0538116591928, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI115532348, Length=381, Percent_Identity=31.496062992126, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI193203271, Length=94, Percent_Identity=30.8510638297872, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=233, Percent_Identity=38.1974248927039, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24656200, Length=415, Percent_Identity=32.0481927710843, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 48472; Mature: 48472

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSKYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVRLN
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHEEC
MNKAGALEVLMPCIQPAHLWMESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRH
CCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
NIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKA
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
YINAFRDLGVFVIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFKTLKDNPDIDVEE
HHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCHHH
IKSWYAAAEEKYEVNKLPISEQEITSSKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPI
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCEEEEEEEEEEECCCCCCCCE
EMSSYGIGISRLVAAIIEANCDEKGIIWPSSVAPFKVSLINLNIHDSKCVELAEMAYKEL
ECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHH
SDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAANNIVELKDRKSGVIEDIEVG
CCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEECCHHHHCCCEEECCCCCCCCCCCCHH
SLMSVL
HHHHCC
>Mature Secondary Structure
MLLSKYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVRLN
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHEEC
MNKAGALEVLMPCIQPAHLWMESGRFDNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRH
CCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
NIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKA
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
YINAFRDLGVFVIPVIADNGPIGGNLSHEFHIIAETGESTIYYDKKFKTLKDNPDIDVEE
HHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCHHH
IKSWYAAAEEKYEVNKLPISEQEITSSKGIEVGHIFYIGSKYSVNMNALINDEYGKLTPI
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCEEEEEEEEEEECCCCCCCCE
EMSSYGIGISRLVAAIIEANCDEKGIIWPSSVAPFKVSLINLNIHDSKCVELAEMAYKEL
ECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHH
SDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAANNIVELKDRKSGVIEDIEVG
CCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEECCHHHHCCCEEECCCCCCCCCCCCHH
SLMSVL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA