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The map label for this gene is rffE [C]

Identifier: 229586601

GI number: 229586601

Start: 453703

End: 454836

Strand: Direct

Name: rffE [C]

Synonym: RAF_ORF0427

Alternate gene names: 229586601

Gene position: 453703-454836 (Clockwise)

Preceding gene: 229586600

Following gene: 229586602

Centisome position: 35.49

GC content: 35.63

Gene sequence:

>1134_bases
ATGCTTAAGGTAATGACGATTGTTGGTACTCGTCCTGAACTTATTAAACTATGCTGCGTGATTTCTGAGTTTGATAAATA
TACTCATCACATCCTAGTTCACACCGGTCAAAATTATGCATATGAATTACATCAGGTTTTTTTTGATGATATGGGAATTA
GAAAACCCGATTATTTCTTAGAAGTAGCAGCAGATAATACGGCAAAATCTATCGGGCTTATTATCGAAAAAGTTGATGCA
GTACTTGAGAAAGAAAAGCCCGACGCCGTTCTATTTTATGGTGATACTAATTCATGTTTATCGGCAATTGCCGCTAAACG
CCGTAAAATTCCGATTTTTCATATGGAAGCGGGAAATCGTTGTTTTGATCAGCGTGTACCTGAAGAAATAAACCGTAAAA
TCATTGATCATATTAGTGACGTTAACATTACGTTAACCGAGCATGCAAGACGTTATTTAATTTTTGAAGGTTTACCGCCT
GAGCTTATTTTTAAATCAGGTTCGCATATGCCTGAGGTCCTTGATCGCTTTATGTCTAAAATATTAAAGTCTGATATTTT
AGATAAATTGTCACTTACCACAAAGCAATATTTCTTAATTAGCTCACATCGTGAGGAAAATGTTGATGTCAAAAATAACC
TGAAAGAATTATTAAGTAGTTTACAAACGCTTATTAAAGAGTATAATTTTCCTGTAATCTTCTCAACACACCCAAGGACT
AAAAAAAGGCTTGAGGATTTAGAGGAGTTTAAAGAACTTGGCGATAAAATAAGATTTTTGCCAGCCTTCGGGTTTACGGA
CTACGTCAAACTTCAGATGAATGCATTTTGTATTTTATCCGATAGCGGTACTATCACTGAAGAGGCATCTATCCTTAATT
TACCTGCTTTAAATATTCGAGAAGCACATGAACGTCCTGAAGGTATGGATGCCGGAACGCTTATTATGTCGGGTTTTAAG
GCTAAACGGGTACTACAATCGGTGAAAGCTATTACGGAAGAACACGAGAATAATAAACGCTTGCAAGAGATCGTGCCGGA
TTATGCAGAAGCAGGACTTGTATCAAAGAAAATATTACGTATAGTTTTAAGTTATGTGGATTATGTTAACCATACAGTAT
GGTTTAAAAAGTAA

Upstream 100 bases:

>100_bases
CTTTTAGAGGATTACACTTCCCATAATACAAAACGTTTAAATTTAGAAGAAGTAAAAGAGTTATTACTGACTCTTGATTA
CGTACAAGAGGAGCTAAAAA

Downstream 100 bases:

>100_bases
TCCTATTCATTATTATCATATCAAAAACATGAAATTTTTTAAATTAATAAAACTTAACACTTTATTTTTAAGTGTTTTTT
TACTAATAACTCTTATATCT

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MLKVMTIVGTRPELIKLCCVISEFDKYTHHILVHTGQNYAYELHQVFFDDMGIRKPDYFLEVAADNTAKSIGLIIEKVDA
VLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNRCFDQRVPEEINRKIIDHISDVNITLTEHARRYLIFEGLPP
ELIFKSGSHMPEVLDRFMSKILKSDILDKLSLTTKQYFLISSHREENVDVKNNLKELLSSLQTLIKEYNFPVIFSTHPRT
KKRLEDLEEFKELGDKIRFLPAFGFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIREAHERPEGMDAGTLIMSGFK
AKRVLQSVKAITEEHENNKRLQEIVPDYAEAGLVSKKILRIVLSYVDYVNHTVWFKK

Sequences:

>Translated_377_residues
MLKVMTIVGTRPELIKLCCVISEFDKYTHHILVHTGQNYAYELHQVFFDDMGIRKPDYFLEVAADNTAKSIGLIIEKVDA
VLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNRCFDQRVPEEINRKIIDHISDVNITLTEHARRYLIFEGLPP
ELIFKSGSHMPEVLDRFMSKILKSDILDKLSLTTKQYFLISSHREENVDVKNNLKELLSSLQTLIKEYNFPVIFSTHPRT
KKRLEDLEEFKELGDKIRFLPAFGFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIREAHERPEGMDAGTLIMSGFK
AKRVLQSVKAITEEHENNKRLQEIVPDYAEAGLVSKKILRIVLSYVDYVNHTVWFKK
>Mature_377_residues
MLKVMTIVGTRPELIKLCCVISEFDKYTHHILVHTGQNYAYELHQVFFDDMGIRKPDYFLEVAADNTAKSIGLIIEKVDA
VLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNRCFDQRVPEEINRKIIDHISDVNITLTEHARRYLIFEGLPP
ELIFKSGSHMPEVLDRFMSKILKSDILDKLSLTTKQYFLISSHREENVDVKNNLKELLSSLQTLIKEYNFPVIFSTHPRT
KKRLEDLEEFKELGDKIRFLPAFGFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIREAHERPEGMDAGTLIMSGFK
AKRVLQSVKAITEEHENNKRLQEIVPDYAEAGLVSKKILRIVLSYVDYVNHTVWFKK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=375, Percent_Identity=27.4666666666667, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 43354; Mature: 43354

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVMTIVGTRPELIKLCCVISEFDKYTHHILVHTGQNYAYELHQVFFDDMGIRKPDYFL
CEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEE
EVAADNTAKSIGLIIEKVDAVLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNR
EEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCH
CFDQRVPEEINRKIIDHISDVNITLTEHARRYLIFEGLPPELIFKSGSHMPEVLDRFMSK
HHHHCCCHHHHHHHHHHHCCCEEEEHHCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHH
ILKSDILDKLSLTTKQYFLISSHREENVDVKNNLKELLSSLQTLIKEYNFPVIFSTHPRT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
KKRLEDLEEFKELGDKIRFLPAFGFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIR
HHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCCEECCCCCCHH
EAHERPEGMDAGTLIMSGFKAKRVLQSVKAITEEHENNKRLQEIVPDYAEAGLVSKKILR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
IVLSYVDYVNHTVWFKK
HHHHHHHHHCCEEEECC
>Mature Secondary Structure
MLKVMTIVGTRPELIKLCCVISEFDKYTHHILVHTGQNYAYELHQVFFDDMGIRKPDYFL
CEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEE
EVAADNTAKSIGLIIEKVDAVLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNR
EEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCH
CFDQRVPEEINRKIIDHISDVNITLTEHARRYLIFEGLPPELIFKSGSHMPEVLDRFMSK
HHHHCCCHHHHHHHHHHHCCCEEEEHHCCCEEEEECCCCHHHHHCCCCCCHHHHHHHHHH
ILKSDILDKLSLTTKQYFLISSHREENVDVKNNLKELLSSLQTLIKEYNFPVIFSTHPRT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
KKRLEDLEEFKELGDKIRFLPAFGFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIR
HHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCCEECCCCCCHH
EAHERPEGMDAGTLIMSGFKAKRVLQSVKAITEEHENNKRLQEIVPDYAEAGLVSKKILR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
IVLSYVDYVNHTVWFKK
HHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA