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The map label for this gene is lpxB [H]

Identifier: 229586584

GI number: 229586584

Start: 434925

End: 436265

Strand: Direct

Name: lpxB [H]

Synonym: RAF_ORF0408

Alternate gene names: 229586584

Gene position: 434925-436265 (Clockwise)

Preceding gene: 229586583

Following gene: 229586586

Centisome position: 34.02

GC content: 32.21

Gene sequence:

>1341_bases
ATGACAAAAATTTACTTTATAGCTGGTGAGGTGTCAGGAGACTTTGTAGGTGGTCGTATAATGCAGCATTTAAAAAACAA
TACAGGAGTACAACTCAATTCACCTGTATCAAGTTTTGTGAATGACGCTGTACAGTTTGTTGGTGTTGGCGGCAAATATA
TGGAGGAAGCCGGTAGCTTTAAAAGCTTATTTCCTATTACTTCCATAAATTTAATGGGTTTTGTAGAAATTTTGCCTCAT
ATTTTTAAGCTTAAAAAATTAATTGATAAAACTGTGGAGGATATAATAAATAGTAAAGCTGATTTATTAATTACCATAGA
TTCACCAGGGTTTACTTATCGTGTGGCAAAGCGAGTAAGGAAACTTTTACCAAAGCTGAAAATGATTCATATCGTTGCAC
CATCAGTTTGGGCATATAAAGAGGGTAGGGCAGTAAAATACGCTAAAATTTATGATTGTTTATTTGCTTTACTACCGTTT
GAACCTCCATATTTTACTAAAGTCGGTCTTGATTGTAGATATATAGGTCATCCGATTATGGAGCAAGAGTTTTATAGTGA
TAAAATAGCTTTACGTGAAGAGTTTAAAATAGATGAGAATGAGAGAGTTTTGTGTGTTACTCTTGGTAGTAGGAATGGTG
AGATTCTAAGGCATTTATCGGTTTTTGTTTCTTCTATTGAAGAAATATTCAAGAGTTGTAATAATCTTAAAGTTATATTT
ACTCTTGCAAATCCTGCTCATGAGGCAATAATAAAACCGTTTCTAGAAGATGTTAAGTTTAATTATTTATTTTCAAGTGA
GAGACTTAAAACTTATGCTGTTGCGGATGTAGCTTTAGCAAAATCCGGTACTAATACTTTAGAGATAGCAGCTTCTGGTA
CTCCTATGATTGTGGCTTATAAAGTTAATCTTATAAGCTTTTTTATTATCAGGCTATTGATAAAAATAAAATATGTTACG
TTGATAAATATTATAGCCGATAAAGAAATAATTCCGGAATTTATCCAATTTAATTGCCGAGCTAATCTTATTAGTAATAA
GCTTCAAGAGTTATTATTTAATTCTAAGAAAGCTTATGAGCAGGTAATAGAAAGCCAAAAAATTTTGCAGCAATTAGGAT
TTAAATCAAACCGATTACAGCTAAATTCAGAAAGTTTTAGACACGATGAATTTAAAGGCAAGCTAGCTAGGTGTACAAAA
GTACGTGAACACAGGCTTAGCTTGGAAAATTCGCTTGTATCAAGTGATCGAGATGACGCTGTACCTTCTTATATAGCTGC
AGCAATTATTAAACAGGAGTTCTTAGAACCTAAAATAAAGTTGTTAAAAGAGAAAGATTAG

Upstream 100 bases:

>100_bases
AAACTCGATAGAAAAAATACATGAGATTACTGAAGTATCTATAAAAGAAATAGAGCGATTACAAACAGAAATTGAGAACC
TTAAAAAATAACTATCTTTA

Downstream 100 bases:

>100_bases
TACTTGAAACCGGTGGTCCACAACATTTTCTGAATTATAATAAACATTAATATTAATATAATAACAAAATATATTTCCAT
GTTTTTTCTTATAGTATTAA

Product: lipid-A-disaccharide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTKIYFIAGEVSGDFVGGRIMQHLKNNTGVQLNSPVSSFVNDAVQFVGVGGKYMEEAGSFKSLFPITSINLMGFVEILPH
IFKLKKLIDKTVEDIINSKADLLITIDSPGFTYRVAKRVRKLLPKLKMIHIVAPSVWAYKEGRAVKYAKIYDCLFALLPF
EPPYFTKVGLDCRYIGHPIMEQEFYSDKIALREEFKIDENERVLCVTLGSRNGEILRHLSVFVSSIEEIFKSCNNLKVIF
TLANPAHEAIIKPFLEDVKFNYLFSSERLKTYAVADVALAKSGTNTLEIAASGTPMIVAYKVNLISFFIIRLLIKIKYVT
LINIIADKEIIPEFIQFNCRANLISNKLQELLFNSKKAYEQVIESQKILQQLGFKSNRLQLNSESFRHDEFKGKLARCTK
VREHRLSLENSLVSSDRDDAVPSYIAAAIIKQEFLEPKIKLLKEKD

Sequences:

>Translated_446_residues
MTKIYFIAGEVSGDFVGGRIMQHLKNNTGVQLNSPVSSFVNDAVQFVGVGGKYMEEAGSFKSLFPITSINLMGFVEILPH
IFKLKKLIDKTVEDIINSKADLLITIDSPGFTYRVAKRVRKLLPKLKMIHIVAPSVWAYKEGRAVKYAKIYDCLFALLPF
EPPYFTKVGLDCRYIGHPIMEQEFYSDKIALREEFKIDENERVLCVTLGSRNGEILRHLSVFVSSIEEIFKSCNNLKVIF
TLANPAHEAIIKPFLEDVKFNYLFSSERLKTYAVADVALAKSGTNTLEIAASGTPMIVAYKVNLISFFIIRLLIKIKYVT
LINIIADKEIIPEFIQFNCRANLISNKLQELLFNSKKAYEQVIESQKILQQLGFKSNRLQLNSESFRHDEFKGKLARCTK
VREHRLSLENSLVSSDRDDAVPSYIAAAIIKQEFLEPKIKLLKEKD
>Mature_445_residues
TKIYFIAGEVSGDFVGGRIMQHLKNNTGVQLNSPVSSFVNDAVQFVGVGGKYMEEAGSFKSLFPITSINLMGFVEILPHI
FKLKKLIDKTVEDIINSKADLLITIDSPGFTYRVAKRVRKLLPKLKMIHIVAPSVWAYKEGRAVKYAKIYDCLFALLPFE
PPYFTKVGLDCRYIGHPIMEQEFYSDKIALREEFKIDENERVLCVTLGSRNGEILRHLSVFVSSIEEIFKSCNNLKVIFT
LANPAHEAIIKPFLEDVKFNYLFSSERLKTYAVADVALAKSGTNTLEIAASGTPMIVAYKVNLISFFIIRLLIKIKYVTL
INIIADKEIIPEFIQFNCRANLISNKLQELLFNSKKAYEQVIESQKILQQLGFKSNRLQLNSESFRHDEFKGKLARCTKV
REHRLSLENSLVSSDRDDAVPSYIAAAIIKQEFLEPKIKLLKEKD

Specific function: Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell [H]

COG id: COG0763

COG function: function code M; Lipid A disaccharide synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RPE3 insert domain [H]

Homologues:

Organism=Escherichia coli, GI1786379, Length=374, Percent_Identity=28.0748663101604, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003835
- InterPro:   IPR022437 [H]

Pfam domain/function: PF02684 LpxB [H]

EC number: =2.4.1.182 [H]

Molecular weight: Translated: 50700; Mature: 50569

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIYFIAGEVSGDFVGGRIMQHLKNNTGVQLNSPVSSFVNDAVQFVGVGGKYMEEAGSF
CCEEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHCCCC
KSLFPITSINLMGFVEILPHIFKLKKLIDKTVEDIINSKADLLITIDSPGFTYRVAKRVR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
KLLPKLKMIHIVAPSVWAYKEGRAVKYAKIYDCLFALLPFEPPYFTKVGLDCRYIGHPIM
HHHHHHHEEEEECCCHHEECCCCEEHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHH
EQEFYSDKIALREEFKIDENERVLCVTLGSRNGEILRHLSVFVSSIEEIFKSCNNLKVIF
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TLANPAHEAIIKPFLEDVKFNYLFSSERLKTYAVADVALAKSGTNTLEIAASGTPMIVAY
EECCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEE
KVNLISFFIIRLLIKIKYVTLINIIADKEIIPEFIQFNCRANLISNKLQELLFNSKKAYE
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHH
QVIESQKILQQLGFKSNRLQLNSESFRHDEFKGKLARCTKVREHRLSLENSLVSSDRDDA
HHHHHHHHHHHHCCCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPSYIAAAIIKQEFLEPKIKLLKEKD
HHHHHHHHHHHHHHHCHHHHHHCCCC
>Mature Secondary Structure 
TKIYFIAGEVSGDFVGGRIMQHLKNNTGVQLNSPVSSFVNDAVQFVGVGGKYMEEAGSF
CEEEEEEECCCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHCCCC
KSLFPITSINLMGFVEILPHIFKLKKLIDKTVEDIINSKADLLITIDSPGFTYRVAKRVR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
KLLPKLKMIHIVAPSVWAYKEGRAVKYAKIYDCLFALLPFEPPYFTKVGLDCRYIGHPIM
HHHHHHHEEEEECCCHHEECCCCEEHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHH
EQEFYSDKIALREEFKIDENERVLCVTLGSRNGEILRHLSVFVSSIEEIFKSCNNLKVIF
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TLANPAHEAIIKPFLEDVKFNYLFSSERLKTYAVADVALAKSGTNTLEIAASGTPMIVAY
EECCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEE
KVNLISFFIIRLLIKIKYVTLINIIADKEIIPEFIQFNCRANLISNKLQELLFNSKKAYE
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHH
QVIESQKILQQLGFKSNRLQLNSESFRHDEFKGKLARCTKVREHRLSLENSLVSSDRDDA
HHHHHHHHHHHHCCCCCCEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPSYIAAAIIKQEFLEPKIKLLKEKD
HHHHHHHHHHHHHHHCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]