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The map label for this gene is lepA

Identifier: 229586525

GI number: 229586525

Start: 369247

End: 371049

Strand: Direct

Name: lepA

Synonym: RAF_ORF0344

Alternate gene names: 229586525

Gene position: 369247-371049 (Clockwise)

Preceding gene: 229586523

Following gene: 229586527

Centisome position: 28.88

GC content: 39.1

Gene sequence:

>1803_bases
ATGAATCATCAAAAATATATAAGGAACTTCTCAATAATCGCCCATATTGATCACGGTAAATCTACGTTAGCCGATAGGTT
AATTGAACATTGTGGCGGCTTGCAGGCTAGGGAAATGAGCCAGCAAGTGCTAGATTCGATGGATATTGAAAAAGAGAGAG
GTATAACTATTAAGGCACAAACCGTGCGGCTTGTATATAAAGCTAAAGACGGTAACACCTACTATTTGAATCTAATGGAC
ACTCCCGGGCATGTCGATTTTGCTTATGAAGTTAGTAGGTCACTTGCTGCTTGTGAAGGCTCGTTATTGGTAGTTGATAG
TACGCAAGGCGTAGCAGCACAAACGCTTGCCAATGTTTACCAAGCAATCGAGAATGATCATGAGATTGTACTGGTGCTTA
ACAAGCTTGACCTACCGGCGTCAGAACCTGAACAGGTTAAGCAGCAAATAGAGGATATAATCGGAATCGATACAAGTGAT
GCTGTGCTAATCTCTGCTAAAAGCGGTATAGGGATTGATTTAGTTTTAGAGGCTATAGTAAATAAATTACCTCCGCCAAA
AGAAAGTAGTTCTGATATTTTGAAAGCTTTGCTTGTTGATAGTTGGTATGACCAATATCTTGGCGTGGTTATTTTAGTAC
GTGTTATTGATGGCTACTTACGTAAGAATATGCGTATTAAAATGATGGCTACTAACTCAGTTTATACCGTTGAGAATGTT
GGCTATTTTACTCCGAAAAAACATATTTCGGATGTTCTGCATGCAGGCGAAATCGGCTTCTTTACTGCGGCTATAAAGCA
AGTAGCGGATTGTAAAGTTGGGGATACGATCACTGATGAAAAAAAGCCTTGTGAGCAAGCTCTACCGGGTTTTAAACCAA
ACTTACCCGTAGTGTTTTGTGGTCTTTATCCAACGGATAGCTCGGAATTTGAGCATTTAAAGGATTCACTGGCTAAATTA
CGCCTTAATGATGCTAGTTTTGAGTATGAAATGGAAAGCTCTTCGGCACTCGGAGTAGGTTTTAGATGCGGCTTTTTAGG
GCTGTTACATTTAGAGATAATCCAAGAACGTTTAAGTAGGGAATTCGATTTAGATTTAATCACTACCGCTCCAAGCGTGG
TGTATAAAATTCATATGCAGGACGGTGAGAACTTAGAGATACATAACCCAGCAGATTTGCCTAATTTACAAAAAATCGAA
TCAATGGAAGAGCCTTGGATTAAGGCAACTATAATGGTACCTGATGAGTTTTTAGGTGCGGTACTATCGCTTTGTACGGA
AAAGAGAGGGATGCAGCTTGATCATAGCTATATAGCAAATAGGGCTAAAATAATTTATAAATTACCGCTAAATGAGATAG
TTTACGATTTCTACGATCGTTTAAAAAGCTGTTCTAAAGGTTATGCAAGTTTTGAATGGCAAATGGATGTTTATGAACCT
TCCGAACTTGTTAAGCTTGGGATTTTGGTTAACGCAGAGGTGGTTGATGCACTATCCACAATCGTACACCGCTCACGTGC
CGAGCAAAGGGGGAGGGCATTATGCGTAAGGTTAAAGGATTTAATACCGAGACAGCAGATTGATATAGCAATTCAAGCAA
GTATAGGTAGTCGTATTATAGCTCGTGAGACTATTAAAGCCCTACGTAAAGATGTTTTATCTAAATGTTACGGCGGTGAT
ATAAGTCGTAAACGTAAATTACTTGAGAAACAAAAAGCAGGTAAAAAGAGAATGAGACAGTACGGTAATATCGAAATTCC
GCAATCTGCGTTTATTGCAGCTTTAAAAATAGGTGATGAATAA

Upstream 100 bases:

>100_bases
ATTTTGATTTTGTTTATATATTATCGTGGTTTAGTGTCATTCTGCGACTTGATCGCGGGATAACAGACAATAAACAAATG
CAAATTATATCACAATAATT

Downstream 100 bases:

>100_bases
AACTTTTTTATTTTATATAAAGAAAATGCTAGACGTTATAATAAAGATTTGTTATAAGGAAGTATCTGAATCTTCCAGAT
GCTTCTAATGTTCCTCGGTA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MNHQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMSQQVLDSMDIEKERGITIKAQTVRLVYKAKDGNTYYLNLMD
TPGHVDFAYEVSRSLAACEGSLLVVDSTQGVAAQTLANVYQAIENDHEIVLVLNKLDLPASEPEQVKQQIEDIIGIDTSD
AVLISAKSGIGIDLVLEAIVNKLPPPKESSSDILKALLVDSWYDQYLGVVILVRVIDGYLRKNMRIKMMATNSVYTVENV
GYFTPKKHISDVLHAGEIGFFTAAIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFCGLYPTDSSEFEHLKDSLAKL
RLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSREFDLDLITTAPSVVYKIHMQDGENLEIHNPADLPNLQKIE
SMEEPWIKATIMVPDEFLGAVLSLCTEKRGMQLDHSYIANRAKIIYKLPLNEIVYDFYDRLKSCSKGYASFEWQMDVYEP
SELVKLGILVNAEVVDALSTIVHRSRAEQRGRALCVRLKDLIPRQQIDIAIQASIGSRIIARETIKALRKDVLSKCYGGD
ISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE

Sequences:

>Translated_600_residues
MNHQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMSQQVLDSMDIEKERGITIKAQTVRLVYKAKDGNTYYLNLMD
TPGHVDFAYEVSRSLAACEGSLLVVDSTQGVAAQTLANVYQAIENDHEIVLVLNKLDLPASEPEQVKQQIEDIIGIDTSD
AVLISAKSGIGIDLVLEAIVNKLPPPKESSSDILKALLVDSWYDQYLGVVILVRVIDGYLRKNMRIKMMATNSVYTVENV
GYFTPKKHISDVLHAGEIGFFTAAIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFCGLYPTDSSEFEHLKDSLAKL
RLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSREFDLDLITTAPSVVYKIHMQDGENLEIHNPADLPNLQKIE
SMEEPWIKATIMVPDEFLGAVLSLCTEKRGMQLDHSYIANRAKIIYKLPLNEIVYDFYDRLKSCSKGYASFEWQMDVYEP
SELVKLGILVNAEVVDALSTIVHRSRAEQRGRALCVRLKDLIPRQQIDIAIQASIGSRIIARETIKALRKDVLSKCYGGD
ISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE
>Mature_600_residues
MNHQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMSQQVLDSMDIEKERGITIKAQTVRLVYKAKDGNTYYLNLMD
TPGHVDFAYEVSRSLAACEGSLLVVDSTQGVAAQTLANVYQAIENDHEIVLVLNKLDLPASEPEQVKQQIEDIIGIDTSD
AVLISAKSGIGIDLVLEAIVNKLPPPKESSSDILKALLVDSWYDQYLGVVILVRVIDGYLRKNMRIKMMATNSVYTVENV
GYFTPKKHISDVLHAGEIGFFTAAIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFCGLYPTDSSEFEHLKDSLAKL
RLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSREFDLDLITTAPSVVYKIHMQDGENLEIHNPADLPNLQKIE
SMEEPWIKATIMVPDEFLGAVLSLCTEKRGMQLDHSYIANRAKIIYKLPLNEIVYDFYDRLKSCSKGYASFEWQMDVYEP
SELVKLGILVNAEVVDALSTIVHRSRAEQRGRALCVRLKDLIPRQQIDIAIQASIGSRIIARETIKALRKDVLSKCYGGD
ISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=599, Percent_Identity=48.2470784641068, Blast_Score=593, Evalue=1e-169,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=41.6107382550336, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=44.6969696969697, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=37.3333333333333, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=41.7910447761194, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=41.7910447761194, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=41.7910447761194, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI217272894, Length=140, Percent_Identity=35, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI217272892, Length=140, Percent_Identity=35, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=41.2844036697248, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=41.2844036697248, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=41.2844036697248, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI223555963, Length=282, Percent_Identity=22.3404255319149, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI94966752, Length=98, Percent_Identity=40.8163265306122, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI5729864, Length=282, Percent_Identity=22.3404255319149, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=57.7181208053691, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=521, Percent_Identity=27.2552783109405, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=31.4102564102564, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=32.258064516129, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789559, Length=223, Percent_Identity=28.2511210762332, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=39.1803278688525, Blast_Score=455, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17506493, Length=187, Percent_Identity=35.2941176470588, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=36.5671641791045, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=36.5671641791045, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=454, Percent_Identity=25.1101321585903, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17552882, Length=139, Percent_Identity=34.5323741007194, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=32.8671328671329, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI32566303, Length=316, Percent_Identity=23.1012658227848, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=43.8127090301003, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=39.5973154362416, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=39.5973154362416, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323098, Length=188, Percent_Identity=36.1702127659575, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=163, Percent_Identity=32.5153374233129, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=158, Percent_Identity=34.1772151898734, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322675, Length=145, Percent_Identity=30.3448275862069, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.5742904841402, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=45.3237410071942, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=36.6013071895425, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=36.6013071895425, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=36.6013071895425, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24582462, Length=150, Percent_Identity=37.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221458488, Length=154, Percent_Identity=36.3636363636364, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357743, Length=139, Percent_Identity=33.8129496402878, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28572034, Length=223, Percent_Identity=29.1479820627803, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_RICAE (C3PMX3)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845026.1
- GeneID:   7815019
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0344
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67209; Mature: 67209

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMSQQVLDSMDIEKERGITIKAQ
CCCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEE
TVRLVYKAKDGNTYYLNLMDTPGHVDFAYEVSRSLAACEGSLLVVDSTQGVAAQTLANVY
EEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
QAIENDHEIVLVLNKLDLPASEPEQVKQQIEDIIGIDTSDAVLISAKSGIGIDLVLEAIV
HHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
NKLPPPKESSSDILKALLVDSWYDQYLGVVILVRVIDGYLRKNMRIKMMATNSVYTVENV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
GYFTPKKHISDVLHAGEIGFFTAAIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFC
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
GLYPTDSSEFEHLKDSLAKLRLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSR
ECCCCCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
EFDLDLITTAPSVVYKIHMQDGENLEIHNPADLPNLQKIESMEEPWIKATIMVPDEFLGA
CCCCEEEECCCEEEEEEEECCCCCEEECCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHH
VLSLCTEKRGMQLDHSYIANRAKIIYKLPLNEIVYDFYDRLKSCSKGYASFEWQMDVYEP
HHHHHHHHCCCCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
SELVKLGILVNAEVVDALSTIVHRSRAEQRGRALCVRLKDLIPRQQIDIAIQASIGSRII
HHHHEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHH
ARETIKALRKDVLSKCYGGDISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MNHQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMSQQVLDSMDIEKERGITIKAQ
CCCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEE
TVRLVYKAKDGNTYYLNLMDTPGHVDFAYEVSRSLAACEGSLLVVDSTQGVAAQTLANVY
EEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
QAIENDHEIVLVLNKLDLPASEPEQVKQQIEDIIGIDTSDAVLISAKSGIGIDLVLEAIV
HHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
NKLPPPKESSSDILKALLVDSWYDQYLGVVILVRVIDGYLRKNMRIKMMATNSVYTVENV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCC
GYFTPKKHISDVLHAGEIGFFTAAIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFC
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEE
GLYPTDSSEFEHLKDSLAKLRLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSR
ECCCCCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCC
EFDLDLITTAPSVVYKIHMQDGENLEIHNPADLPNLQKIESMEEPWIKATIMVPDEFLGA
CCCCEEEECCCEEEEEEEECCCCCEEECCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHH
VLSLCTEKRGMQLDHSYIANRAKIIYKLPLNEIVYDFYDRLKSCSKGYASFEWQMDVYEP
HHHHHHHHCCCCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
SELVKLGILVNAEVVDALSTIVHRSRAEQRGRALCVRLKDLIPRQQIDIAIQASIGSRII
HHHHEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHH
ARETIKALRKDVLSKCYGGDISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA