Click here to switch to the map view.

The map label for this gene is tolC [H]

Identifier: 229586472

GI number: 229586472

Start: 309597

End: 310958

Strand: Direct

Name: tolC [H]

Synonym: RAF_ORF0284

Alternate gene names: 229586472

Gene position: 309597-310958 (Clockwise)

Preceding gene: 229586470

Following gene: 229586473

Centisome position: 24.21

GC content: 36.71

Gene sequence:

>1362_bases
ATGCGTAAATTAATTACATTTATTGTTACATTATTACTAACTAGTTCGGTTGCAGCAGTTGATTTGCAGGGAGCTTTAAC
TGAAGGATATAAGAATAATGAGGAGCTGAAAGCTGCTAGAATTAAGTTTTTAGACTCGATTGAGCAGTTTCCGCGAGCTT
TTTCGGGGTTTATGCCTAGTGCCGGATTACAGATTAATAGACAACATAATAAGACTAAATATAATAAAAAATATGCTGAT
AGGCTTGGTCTTACTCCAAGGGACACAGATAGTGATCAAGGTGCATTAACAATTCAACAGTCTCTATTTAATGGTGGTTC
TAGTGTTGCGGCTCTTAAAGCTGCTCAAGCAGGATTTAGAGCTGCACGGGGTGCATATTATGCCGGTGAACAAAAAATAT
TATTAAATTTAATAACTGCGTATCTTGATTGTTTTGAGAGCAAAGAGAAATATGATATTTCCGAAAGTAGAGTTCGCACT
AACATACAACAAGTTAACACCGTTGAGGAAAAATTAAGACTTGGAGAAGCAACGGAAGTAGATATAGCAACCGCAAGAGC
GGGGCTTGCAGCAGCAGAAACGAATAAACTAGCTGCTTATGCCGATTTTCAAGGAAAAAAAGCAAATTTTATTAGAGTAT
TTGGAATAGAGCCGACTAATATAACTATGCCTGAGTTACCTAAAATGTTACCTGCTTCATTAGATGAGTTAACTGGAAGA
GCTGCTAAACTCAATCCTGATATTGATTCGGCAAGGCATAATGTAACTGCGGCTAAAGCTTTAGAAATGGCAGAAAAAGG
GAAATTATTACCGCAAGTAAGTGTGCAACTGCAATCCGGTAGAACATACTACAATCCACAAGGTGATATAAATGCTATAA
ATAATAGAAGCTATACTACTACTCTTTCGGTAAATATCCCTATTTATCCTGAGGGAGGAGCTCAATATTCAAGAATCAGA
TCGGCTAAAAATCAAACAAGAAATAGTGCAGTTCAACTTGATAATGTGATAAAGCAGACCCAAGCAGGGGTTGTAAGCGT
GTGGGAGGGATTTGAAGCAGCAAAATCTCGTATTGTTGCCGCTAATCAAGGAGTGGATGCTGCTCAAATATCATATGACG
GTACTGTGCAAGAGGAAATAGTCGGATCTAAAACAATATTAGATGTTTTAACTGCCGCAGAAAAATTATACGAAGCAAAA
ATAACTCGTGTGGATGCGTATAAATCTTCAGTACTTGCGGCATATCAAATGAAATTGTTAACTGGCGAGCTAACGGCTCA
AAGTTTAAAACTTAAAGTAAAATATTTTAGTCCTGAAGAAGAGTTTAAGACTATTAAAAAGAAAATGTTTATAGGTTTCT
AA

Upstream 100 bases:

>100_bases
CATTGCTACAATAGATTTATATTGTTTTATTAGGATATAGGTATATTCTTGAAAGATTAAACTAATCTAACTAACATATT
ATCAATGATTCAAGAAATTT

Downstream 100 bases:

>100_bases
CGTGAGTAAGGACAATAAAAAGAACCAAGATATGTCGATAGAAGACATATTAAAATCGATTAAAGGAGTAATAAACGAGC
GTAAAAATCCTATTCATGAA

Product: Type I secretion outer membrane protein TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MRKLITFIVTLLLTSSVAAVDLQGALTEGYKNNEELKAARIKFLDSIEQFPRAFSGFMPSAGLQINRQHNKTKYNKKYAD
RLGLTPRDTDSDQGALTIQQSLFNGGSSVAALKAAQAGFRAARGAYYAGEQKILLNLITAYLDCFESKEKYDISESRVRT
NIQQVNTVEEKLRLGEATEVDIATARAGLAAAETNKLAAYADFQGKKANFIRVFGIEPTNITMPELPKMLPASLDELTGR
AAKLNPDIDSARHNVTAAKALEMAEKGKLLPQVSVQLQSGRTYYNPQGDINAINNRSYTTTLSVNIPIYPEGGAQYSRIR
SAKNQTRNSAVQLDNVIKQTQAGVVSVWEGFEAAKSRIVAANQGVDAAQISYDGTVQEEIVGSKTILDVLTAAEKLYEAK
ITRVDAYKSSVLAAYQMKLLTGELTAQSLKLKVKYFSPEEEFKTIKKKMFIGF

Sequences:

>Translated_453_residues
MRKLITFIVTLLLTSSVAAVDLQGALTEGYKNNEELKAARIKFLDSIEQFPRAFSGFMPSAGLQINRQHNKTKYNKKYAD
RLGLTPRDTDSDQGALTIQQSLFNGGSSVAALKAAQAGFRAARGAYYAGEQKILLNLITAYLDCFESKEKYDISESRVRT
NIQQVNTVEEKLRLGEATEVDIATARAGLAAAETNKLAAYADFQGKKANFIRVFGIEPTNITMPELPKMLPASLDELTGR
AAKLNPDIDSARHNVTAAKALEMAEKGKLLPQVSVQLQSGRTYYNPQGDINAINNRSYTTTLSVNIPIYPEGGAQYSRIR
SAKNQTRNSAVQLDNVIKQTQAGVVSVWEGFEAAKSRIVAANQGVDAAQISYDGTVQEEIVGSKTILDVLTAAEKLYEAK
ITRVDAYKSSVLAAYQMKLLTGELTAQSLKLKVKYFSPEEEFKTIKKKMFIGF
>Mature_453_residues
MRKLITFIVTLLLTSSVAAVDLQGALTEGYKNNEELKAARIKFLDSIEQFPRAFSGFMPSAGLQINRQHNKTKYNKKYAD
RLGLTPRDTDSDQGALTIQQSLFNGGSSVAALKAAQAGFRAARGAYYAGEQKILLNLITAYLDCFESKEKYDISESRVRT
NIQQVNTVEEKLRLGEATEVDIATARAGLAAAETNKLAAYADFQGKKANFIRVFGIEPTNITMPELPKMLPASLDELTGR
AAKLNPDIDSARHNVTAAKALEMAEKGKLLPQVSVQLQSGRTYYNPQGDINAINNRSYTTTLSVNIPIYPEGGAQYSRIR
SAKNQTRNSAVQLDNVIKQTQAGVVSVWEGFEAAKSRIVAANQGVDAAQISYDGTVQEEIVGSKTILDVLTAAEKLYEAK
ITRVDAYKSSVLAAYQMKLLTGELTAQSLKLKVKYFSPEEEFKTIKKKMFIGF

Specific function: Involved in the efflux of toxic and relatively hydrophobic compounds. Influences survival inside the host [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=414, Percent_Identity=23.1884057971014, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49728; Mature: 49728

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLITFIVTLLLTSSVAAVDLQGALTEGYKNNEELKAARIKFLDSIEQFPRAFSGFMPS
CHHHHHHHHHHHHHCCHHEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGLQINRQHNKTKYNKKYADRLGLTPRDTDSDQGALTIQQSLFNGGSSVAALKAAQAGFR
CCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHH
AARGAYYAGEQKILLNLITAYLDCFESKEKYDISESRVRTNIQQVNTVEEKLRLGEATEV
HHCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
DIATARAGLAAAETNKLAAYADFQGKKANFIRVFGIEPTNITMPELPKMLPASLDELTGR
EHHHHHCCCHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHCCC
AAKLNPDIDSARHNVTAAKALEMAEKGKLLPQVSVQLQSGRTYYNPQGDINAINNRSYTT
CEECCCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCEEECCCEEEE
TLSVNIPIYPEGGAQYSRIRSAKNQTRNSAVQLDNVIKQTQAGVVSVWEGFEAAKSRIVA
EEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEE
ANQGVDAAQISYDGTVQEEIVGSKTILDVLTAAEKLYEAKITRVDAYKSSVLAAYQMKLL
ECCCCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGELTAQSLKLKVKYFSPEEEFKTIKKKMFIGF
HHHHCCEEEEEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLITFIVTLLLTSSVAAVDLQGALTEGYKNNEELKAARIKFLDSIEQFPRAFSGFMPS
CHHHHHHHHHHHHHCCHHEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGLQINRQHNKTKYNKKYADRLGLTPRDTDSDQGALTIQQSLFNGGSSVAALKAAQAGFR
CCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHH
AARGAYYAGEQKILLNLITAYLDCFESKEKYDISESRVRTNIQQVNTVEEKLRLGEATEV
HHCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCE
DIATARAGLAAAETNKLAAYADFQGKKANFIRVFGIEPTNITMPELPKMLPASLDELTGR
EHHHHHCCCHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCHHHHCCC
AAKLNPDIDSARHNVTAAKALEMAEKGKLLPQVSVQLQSGRTYYNPQGDINAINNRSYTT
CEECCCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCEEECCCEEEE
TLSVNIPIYPEGGAQYSRIRSAKNQTRNSAVQLDNVIKQTQAGVVSVWEGFEAAKSRIVA
EEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEE
ANQGVDAAQISYDGTVQEEIVGSKTILDVLTAAEKLYEAKITRVDAYKSSVLAAYQMKLL
ECCCCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGELTAQSLKLKVKYFSPEEEFKTIKKKMFIGF
HHHHCCEEEEEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]