Click here to switch to the map view.

The map label for this gene is rrmJ

Identifier: 229586405

GI number: 229586405

Start: 215211

End: 215894

Strand: Direct

Name: rrmJ

Synonym: RAF_ORF0196

Alternate gene names: 229586405

Gene position: 215211-215894 (Clockwise)

Preceding gene: 229586404

Following gene: 229586407

Centisome position: 16.83

GC content: 33.63

Gene sequence:

>684_bases
ATGACAAATAATTTAAGCGGTTATAGAAATAAATTCGTTAGAGTTAAGACTTCTAAAAAACGCACCGTCTCTTCTAGTAA
TTGGCTTAGACGCCAATTAAACGATCCGTATGTTGCAAAAGCACGTATGGACGGCTTTAGGTCGAGAGCTGCATATAAAC
TACTTGAAATTCATGAAAAATTTAAACTTTTTACTCCTAACATGAAAATTGTTGATTTAGGAGCAGCTCCTGGGGGATGG
AGTCAAGTAGCTTCCAAGCTGATTAAAGCTTCGGATAATAACCTAAATAATAAAATAATCTCTATCGATGTGTTAGAAAT
TGAACATGTTGCTGGAGTTGAATTTGTTCAAAAAGACTTTTTTGAAGCAGATACGGAAGAATTAATTATTCAGGCTTTAG
ACGGTAGAGCTGATATAGTAATGAGTGATATGGCATCAAATACCATAGGTCATAAAGCTACAGACCATATAAGAACCTTA
CTTTTATGTGAGCAAGCTTTTGAATTTGCTTTAAAAGTACTAAAACCCTCCGGTCATTTTATTGCTAAAATTTTTCGTGG
CGGTGCAGAAAATGAATTATTATATAAAGTAAAGCGTGAATTTAAAACAGTGAAACATTTTAAACCTTCATCCAGCCGCA
GTGAATCTACGGAAATCTATTTAGTTGCTCTAAATAAGAAATAA

Upstream 100 bases:

>100_bases
TATTAACGGCTTTTGTGCCTCATCTTGTGGATTCCTTGCTCTTTTCCAGATTAAACTTAGTCTGCTTATTCTTCATTTAT
TTATAAGTATAAAAGATTTT

Downstream 100 bases:

>100_bases
TGAATATGAAAGGCGAAAAGTGATTTCTGCCTATAAATTTATTTTTTTATTTAACATATTAGAGGATATAGATGATAATT
ACTTATATCTCCTGTTGTTT

Product: Ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTNNLSGYRNKFVRVKTSKKRTVSSSNWLRRQLNDPYVAKARMDGFRSRAAYKLLEIHEKFKLFTPNMKIVDLGAAPGGW
SQVASKLIKASDNNLNNKIISIDVLEIEHVAGVEFVQKDFFEADTEELIIQALDGRADIVMSDMASNTIGHKATDHIRTL
LLCEQAFEFALKVLKPSGHFIAKIFRGGAENELLYKVKREFKTVKHFKPSSSRSESTEIYLVALNKK

Sequences:

>Translated_227_residues
MTNNLSGYRNKFVRVKTSKKRTVSSSNWLRRQLNDPYVAKARMDGFRSRAAYKLLEIHEKFKLFTPNMKIVDLGAAPGGW
SQVASKLIKASDNNLNNKIISIDVLEIEHVAGVEFVQKDFFEADTEELIIQALDGRADIVMSDMASNTIGHKATDHIRTL
LLCEQAFEFALKVLKPSGHFIAKIFRGGAENELLYKVKREFKTVKHFKPSSSRSESTEIYLVALNKK
>Mature_226_residues
TNNLSGYRNKFVRVKTSKKRTVSSSNWLRRQLNDPYVAKARMDGFRSRAAYKLLEIHEKFKLFTPNMKIVDLGAAPGGWS
QVASKLIKASDNNLNNKIISIDVLEIEHVAGVEFVQKDFFEADTEELIIQALDGRADIVMSDMASNTIGHKATDHIRTLL
LCEQAFEFALKVLKPSGHFIAKIFRGGAENELLYKVKREFKTVKHFKPSSSRSESTEIYLVALNKK

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=211, Percent_Identity=35.5450236966825, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI29029591, Length=191, Percent_Identity=36.6492146596859, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI29029589, Length=191, Percent_Identity=36.6492146596859, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7110661, Length=191, Percent_Identity=36.6492146596859, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI194097365, Length=194, Percent_Identity=32.4742268041237, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1789569, Length=216, Percent_Identity=43.5185185185185, Blast_Score=177, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17553474, Length=218, Percent_Identity=33.0275229357798, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17554650, Length=196, Percent_Identity=35.7142857142857, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71987550, Length=194, Percent_Identity=32.9896907216495, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71987561, Length=194, Percent_Identity=32.9896907216495, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17553860, Length=194, Percent_Identity=32.9896907216495, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=287, Percent_Identity=28.9198606271777, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6319535, Length=196, Percent_Identity=34.6938775510204, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=33.5078534031414, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356387, Length=214, Percent_Identity=37.8504672897196, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24648639, Length=188, Percent_Identity=36.1702127659575, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18859957, Length=190, Percent_Identity=33.1578947368421, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24647580, Length=196, Percent_Identity=34.1836734693878, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_RICAE (C3PMK3)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844906.1
- ProteinModelPortal:   C3PMK3
- GeneID:   7815205
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0196
- OMA:   SDMAANT
- ProtClustDB:   CLSK870770
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 25738; Mature: 25607

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: ACT_SITE 183-183 BINDING 78-78 BINDING 80-80 BINDING 103-103 BINDING 119-119 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNLSGYRNKFVRVKTSKKRTVSSSNWLRRQLNDPYVAKARMDGFRSRAAYKLLEIHEK
CCCCCCCHHCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FKLFTPNMKIVDLGAAPGGWSQVASKLIKASDNNLNNKIISIDVLEIEHVAGVEFVQKDF
HHEECCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHH
FEADTEELIIQALDGRADIVMSDMASNTIGHKATDHIRTLLLCEQAFEFALKVLKPSGHF
HCCCHHHHHHHHHCCCHHEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IAKIFRGGAENELLYKVKREFKTVKHFKPSSSRSESTEIYLVALNKK
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TNNLSGYRNKFVRVKTSKKRTVSSSNWLRRQLNDPYVAKARMDGFRSRAAYKLLEIHEK
CCCCCCHHCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FKLFTPNMKIVDLGAAPGGWSQVASKLIKASDNNLNNKIISIDVLEIEHVAGVEFVQKDF
HHEECCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHH
FEADTEELIIQALDGRADIVMSDMASNTIGHKATDHIRTLLLCEQAFEFALKVLKPSGHF
HCCCHHHHHHHHHCCCHHEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IAKIFRGGAENELLYKVKREFKTVKHFKPSSSRSESTEIYLVALNKK
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA