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The map label for this gene is trmD

Identifier: 229586349

GI number: 229586349

Start: 153126

End: 153830

Strand: Direct

Name: trmD

Synonym: RAF_ORF0140

Alternate gene names: 229586349

Gene position: 153126-153830 (Clockwise)

Preceding gene: 229586348

Following gene: 229586350

Centisome position: 11.98

GC content: 37.59

Gene sequence:

>705_bases
ATGTCTATACTTCATGCCACAATCTTAACCGTTTTTCCTGAAATGTTTCCTGGAACTCTAGGACATTCTTTAGCAGGACA
AGCACTAAATAAAAATATTTGGTCATATGACGTTATTAATATTCGAGATTTCGGTTTAACTAAGCATAAAAATATTGATG
ATGAGGCTTATGGCGGTGGTAACGGGCTTATAATGCGTCCTGATGTTCTAGGCAGTAGCATAGATCATGCTCTTGCTTTA
AATCCTAATGCTGAAATGTACTATCCTTCACCTCGCGGTAGAGTCTTTACCCAAAGCTTTGCTAAAGAGATGTTAAAAAA
TAAAAACCTCATATTCTTATGTGGACGTTACGAAGGGATTGACGAACGTGTAATTGAAGAGTATAATGTTAAAGAAATTA
GTGTAGGCGACTACATTTTATCCGGCGGAGAAATACCTACCCTCACCATTCTCGATTGCTTAATTAGACTACTGCCAGGT
GTTTTAATGAACCAAAATACCTTATCTTCTGAGTCTTTTGAAGAAGACGGGGAATTTAAGGGTGGGCTTGAATGCAGCCT
ATATACAAGACCTGAAATTTGGCGTGATCGAGCTGTACCGAGTGTTTTGTTATCCGGTAACCACAGACTAATTAATGAAT
GGAAAAAAGAGCAATCACATATGATTACTAAATTACGCCGTCCGGAGTTACTTAAAGATTTATAG

Upstream 100 bases:

>100_bases
TAAAAAACAAAGAAAAGAAGATTATAGGACAGGTGGGTGGGATAAATAATTAAATTATAATTTTATTGCGTTAATTGGTA
CTGTCTACTATTTTAAACGT

Downstream 100 bases:

>100_bases
AGTTAAGGAGTTAAAAAATGAATATTATTGACCGCTTTGAACAGGAAAATATTTCAAAGCGTACTGCAAATAAAAAAATT
CCTGATTTTGAAGCCGGCGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSILHATILTVFPEMFPGTLGHSLAGQALNKNIWSYDVINIRDFGLTKHKNIDDEAYGGGNGLIMRPDVLGSSIDHALAL
NPNAEMYYPSPRGRVFTQSFAKEMLKNKNLIFLCGRYEGIDERVIEEYNVKEISVGDYILSGGEIPTLTILDCLIRLLPG
VLMNQNTLSSESFEEDGEFKGGLECSLYTRPEIWRDRAVPSVLLSGNHRLINEWKKEQSHMITKLRRPELLKDL

Sequences:

>Translated_234_residues
MSILHATILTVFPEMFPGTLGHSLAGQALNKNIWSYDVINIRDFGLTKHKNIDDEAYGGGNGLIMRPDVLGSSIDHALAL
NPNAEMYYPSPRGRVFTQSFAKEMLKNKNLIFLCGRYEGIDERVIEEYNVKEISVGDYILSGGEIPTLTILDCLIRLLPG
VLMNQNTLSSESFEEDGEFKGGLECSLYTRPEIWRDRAVPSVLLSGNHRLINEWKKEQSHMITKLRRPELLKDL
>Mature_233_residues
SILHATILTVFPEMFPGTLGHSLAGQALNKNIWSYDVINIRDFGLTKHKNIDDEAYGGGNGLIMRPDVLGSSIDHALALN
PNAEMYYPSPRGRVFTQSFAKEMLKNKNLIFLCGRYEGIDERVIEEYNVKEISVGDYILSGGEIPTLTILDCLIRLLPGV
LMNQNTLSSESFEEDGEFKGGLECSLYTRPEIWRDRAVPSVLLSGNHRLINEWKKEQSHMITKLRRPELLKDL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=228, Percent_Identity=42.5438596491228, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_RICAE (C3PME7)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844850.1
- ProteinModelPortal:   C3PME7
- SMR:   C3PME7
- GeneID:   7815389
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0140
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26413; Mature: 26282

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILHATILTVFPEMFPGTLGHSLAGQALNKNIWSYDVINIRDFGLTKHKNIDDEAYGGG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCC
NGLIMRPDVLGSSIDHALALNPNAEMYYPSPRGRVFTQSFAKEMLKNKNLIFLCGRYEGI
CCEEECCHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
DERVIEEYNVKEISVGDYILSGGEIPTLTILDCLIRLLPGVLMNQNTLSSESFEEDGEFK
HHHHHHHCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GGLECSLYTRPEIWRDRAVPSVLLSGNHRLINEWKKEQSHMITKLRRPELLKDL
CCCEEEEECCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
SILHATILTVFPEMFPGTLGHSLAGQALNKNIWSYDVINIRDFGLTKHKNIDDEAYGGG
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCC
NGLIMRPDVLGSSIDHALALNPNAEMYYPSPRGRVFTQSFAKEMLKNKNLIFLCGRYEGI
CCEEECCHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
DERVIEEYNVKEISVGDYILSGGEIPTLTILDCLIRLLPGVLMNQNTLSSESFEEDGEFK
HHHHHHHCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GGLECSLYTRPEIWRDRAVPSVLLSGNHRLINEWKKEQSHMITKLRRPELLKDL
CCCEEEEECCCHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA