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The map label for this gene is cysS

Identifier: 229586323

GI number: 229586323

Start: 111304

End: 112683

Strand: Direct

Name: cysS

Synonym: RAF_ORF0106

Alternate gene names: 229586323

Gene position: 111304-112683 (Clockwise)

Preceding gene: 229586321

Following gene: 229586324

Centisome position: 8.71

GC content: 33.26

Gene sequence:

>1380_bases
ATGCAAATTCAATTTCACTTATATAACACTCTTAGTCGTACTAAAGAGGTTTTTAATCCTCAAGATCAAGCTAACGTAAA
AATGTATGTATGTGGACCGACTGTTTATTATAATCCTCATATAGGCAATAGTAGGTCGGGAGTAGTATATGATTTACTTT
ATCGTATAGTTATAAAAATATTCGGTGAGAAGGCAGTAAAATATGTACGTAACATTACGGATGTTGACGATAAAATTATC
GATAGAGCTGCACTACTTGGTGTTACTATTGATGAGTTGACTGATAAAGTTACGAAAGAGTTTCATAAAAATATGGCATA
TTTAGGTTGTATGCTACCTAGTATTGAGCCGAAAGCTACAAAGCATATTGATGTAATGATCGCAATAATAGAACGCTTAA
TAGCAAAAGATCACGCATATATTGCTGATAATCATGTTTATTTTGATGTTTTATCAGCGCCAAATTATACGGAATTATCT
AACCGAAATTTAGAAGAAATGTTTGAGGGAGTGCATGTAGAAAATAGTAAAACCAAAAAAAATCCACAAGATTTTGTATT
ATGGAAACCGGCAAAGCAAAATGAATCGGCAAATATGAATTTTGAAAGCCCCTGGGGACTTGGTCGCCCTGGATGGCATA
TTGAATGTTCAGCCATGAGCTACAAATATTTAGGTGAGAATTTTGATATTCACGGCGGCGGAGCAGATTTAATCTTTCCG
CATCATACCAATGAGATTGCTCAAAGTAGATGTGCTTTTCCAAGTTCTACCTATGCTAAATATTGGGTACATAATGGGTT
TCTAACGGTTAACGGTGAGAAAATGAGTAAATCTCTTGGTAATTTTATTACTGTAAGGGATTTAATGGATAAACAAATTC
AAGGAGAAGTAGTAAGGTTATTTTTATTAAGTAGTCACTATAGGCGTCCTCTTGATTATAATGATAAAGCTATAGAAGAT
GCTAAGAAAACTTTAAATTATTGGTATAGAGCTATAGAAAATATTAATGTGCAGAAAATAGATTTACCTCATGATTTCAT
GCAAAGCTTACTTGATGATATGAATACGCCGCTTGCCGTTAAAATCATTAATGACTACGCTAAAGGTGTTTTTATTTCTA
AAACTGAAGAAGAAAGGCAGCTTAACGCCTCTGCTATTATTACTTGTGCTAATTTTATTGGTTTAATGAATAAGACTCCA
AACGAGTGGTTTAATAGCGGTGTGGATGAACTCTATGTAAATGAGCTTGTAAATAAGCGTTTGGAAGCAAAAAAACAAAA
AAATTGGTTATTAGCCGATCAAATTCGTAACCAGTTATTAGAGGAAAAAATAATTCTAGAAGATCGACCTGATGGTACTA
CTATTTGGCGAAAAGAATAA

Upstream 100 bases:

>100_bases
AAGCTAATTTATACTCCGAATTTTAAACTTTTTTCAAAAGGAGTATGTAGTAATACTACTTTTGAAAGAAATGTACTTCA
GTTTTTGGTAAATATAAAAT

Downstream 100 bases:

>100_bases
AAGCTTGTAAATTACGCAATATGCGTATATAATTTATGGGCTTGTAAGTCGTCATTGCGAGGAAATTATGTAATAATTGA
CGAAGCAATCTCACAGGATA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MQIQFHLYNTLSRTKEVFNPQDQANVKMYVCGPTVYYNPHIGNSRSGVVYDLLYRIVIKIFGEKAVKYVRNITDVDDKII
DRAALLGVTIDELTDKVTKEFHKNMAYLGCMLPSIEPKATKHIDVMIAIIERLIAKDHAYIADNHVYFDVLSAPNYTELS
NRNLEEMFEGVHVENSKTKKNPQDFVLWKPAKQNESANMNFESPWGLGRPGWHIECSAMSYKYLGENFDIHGGGADLIFP
HHTNEIAQSRCAFPSSTYAKYWVHNGFLTVNGEKMSKSLGNFITVRDLMDKQIQGEVVRLFLLSSHYRRPLDYNDKAIED
AKKTLNYWYRAIENINVQKIDLPHDFMQSLLDDMNTPLAVKIINDYAKGVFISKTEEERQLNASAIITCANFIGLMNKTP
NEWFNSGVDELYVNELVNKRLEAKKQKNWLLADQIRNQLLEEKIILEDRPDGTTIWRKE

Sequences:

>Translated_459_residues
MQIQFHLYNTLSRTKEVFNPQDQANVKMYVCGPTVYYNPHIGNSRSGVVYDLLYRIVIKIFGEKAVKYVRNITDVDDKII
DRAALLGVTIDELTDKVTKEFHKNMAYLGCMLPSIEPKATKHIDVMIAIIERLIAKDHAYIADNHVYFDVLSAPNYTELS
NRNLEEMFEGVHVENSKTKKNPQDFVLWKPAKQNESANMNFESPWGLGRPGWHIECSAMSYKYLGENFDIHGGGADLIFP
HHTNEIAQSRCAFPSSTYAKYWVHNGFLTVNGEKMSKSLGNFITVRDLMDKQIQGEVVRLFLLSSHYRRPLDYNDKAIED
AKKTLNYWYRAIENINVQKIDLPHDFMQSLLDDMNTPLAVKIINDYAKGVFISKTEEERQLNASAIITCANFIGLMNKTP
NEWFNSGVDELYVNELVNKRLEAKKQKNWLLADQIRNQLLEEKIILEDRPDGTTIWRKE
>Mature_459_residues
MQIQFHLYNTLSRTKEVFNPQDQANVKMYVCGPTVYYNPHIGNSRSGVVYDLLYRIVIKIFGEKAVKYVRNITDVDDKII
DRAALLGVTIDELTDKVTKEFHKNMAYLGCMLPSIEPKATKHIDVMIAIIERLIAKDHAYIADNHVYFDVLSAPNYTELS
NRNLEEMFEGVHVENSKTKKNPQDFVLWKPAKQNESANMNFESPWGLGRPGWHIECSAMSYKYLGENFDIHGGGADLIFP
HHTNEIAQSRCAFPSSTYAKYWVHNGFLTVNGEKMSKSLGNFITVRDLMDKQIQGEVVRLFLLSSHYRRPLDYNDKAIED
AKKTLNYWYRAIENINVQKIDLPHDFMQSLLDDMNTPLAVKIINDYAKGVFISKTEEERQLNASAIITCANFIGLMNKTP
NEWFNSGVDELYVNELVNKRLEAKKQKNWLLADQIRNQLLEEKIILEDRPDGTTIWRKE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=318, Percent_Identity=40.5660377358491, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI10835051, Length=287, Percent_Identity=33.1010452961672, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI21269877, Length=287, Percent_Identity=33.1010452961672, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI303304987, Length=287, Percent_Identity=33.1010452961672, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI62240992, Length=287, Percent_Identity=33.1010452961672, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1786737, Length=468, Percent_Identity=45.0854700854701, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17509795, Length=252, Percent_Identity=36.1111111111111, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17509797, Length=251, Percent_Identity=36.2549800796813, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324082, Length=336, Percent_Identity=31.8452380952381, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI19922218, Length=490, Percent_Identity=34.0816326530612, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI19922364, Length=252, Percent_Identity=37.3015873015873, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_RICAE (C3PMC1)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844824.1
- ProteinModelPortal:   C3PMC1
- GeneID:   7815358
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0106
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52974; Mature: 52974

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIQFHLYNTLSRTKEVFNPQDQANVKMYVCGPTVYYNPHIGNSRSGVVYDLLYRIVIKI
CEEEEEEHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHH
FGEKAVKYVRNITDVDDKIIDRAALLGVTIDELTDKVTKEFHKNMAYLGCMLPSIEPKAT
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
KHIDVMIAIIERLIAKDHAYIADNHVYFDVLSAPNYTELSNRNLEEMFEGVHVENSKTKK
HHHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCC
NPQDFVLWKPAKQNESANMNFESPWGLGRPGWHIECSAMSYKYLGENFDIHGGGADLIFP
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECEEHHHCCCCEEECCCCCEEECC
HHTNEIAQSRCAFPSSTYAKYWVHNGFLTVNGEKMSKSLGNFITVRDLMDKQIQGEVVRL
CCCHHHHHHHCCCCCCCCHHHEEECCEEEECHHHHHHHHCCEEEHHHHHHHHHHHHHHHH
FLLSSHYRRPLDYNDKAIEDAKKTLNYWYRAIENINVQKIDLPHDFMQSLLDDMNTPLAV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCEEE
KIINDYAKGVFISKTEEERQLNASAIITCANFIGLMNKTPNEWFNSGVDELYVNELVNKR
EEEHHHHCCEEEECCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
LEAKKQKNWLLADQIRNQLLEEKIILEDRPDGTTIWRKE
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIQFHLYNTLSRTKEVFNPQDQANVKMYVCGPTVYYNPHIGNSRSGVVYDLLYRIVIKI
CEEEEEEHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHH
FGEKAVKYVRNITDVDDKIIDRAALLGVTIDELTDKVTKEFHKNMAYLGCMLPSIEPKAT
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
KHIDVMIAIIERLIAKDHAYIADNHVYFDVLSAPNYTELSNRNLEEMFEGVHVENSKTKK
HHHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCC
NPQDFVLWKPAKQNESANMNFESPWGLGRPGWHIECSAMSYKYLGENFDIHGGGADLIFP
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECEEHHHCCCCEEECCCCCEEECC
HHTNEIAQSRCAFPSSTYAKYWVHNGFLTVNGEKMSKSLGNFITVRDLMDKQIQGEVVRL
CCCHHHHHHHCCCCCCCCHHHEEECCEEEECHHHHHHHHCCEEEHHHHHHHHHHHHHHHH
FLLSSHYRRPLDYNDKAIEDAKKTLNYWYRAIENINVQKIDLPHDFMQSLLDDMNTPLAV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCEEE
KIINDYAKGVFISKTEEERQLNASAIITCANFIGLMNKTPNEWFNSGVDELYVNELVNKR
EEEHHHHCCEEEECCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
LEAKKQKNWLLADQIRNQLLEEKIILEDRPDGTTIWRKE
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA