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The map label for this gene is sfcA

Identifier: 229259764

GI number: 229259764

Start: 820200

End: 821888

Strand: Reverse

Name: sfcA

Synonym: VC0395_A0809

Alternate gene names: 229259764

Gene position: 821888-820200 (Counterclockwise)

Preceding gene: 147673379

Following gene: 147675734

Centisome position: 27.18

GC content: 50.74

Gene sequence:

>1689_bases
ATGAATAACGATAAACGTCCGCTGTATATTTCCTACGCAGGCCCAGCTCTTCTCAGTACTCCTCTGCTCAACAAAGGTAG
TGCCTTTAGTGCGGAAGAACGTGCTTCTTTTAACCTAGAAGGTTTACTGCCCGAAGCGACGGAAACCATTCAAGAACAAG
TGGTACGCGCATACCAACAGTACCGTGGTTTTGAAAGCGACATGGATAAGCATATTTATCTGCGCAACATTCAAGACACC
AACGAGACCCTATTCTACCGCTTGGTGCAGAACCATATTTCCGAGATGATGCCAATCATCTATACCCCGACAGTGGGTGC
GGCGTGTGAGAACTTCTCCAACATTTATCGCCGTGGCCGTGGCTTGTTTATCTCTTACGCTAACCGTGATCGTATTGATG
ACTTGTTGAACAATGCCGCCAACCACAACGTCAAAGTGATTGTGGTGACCGATGGCGAGCGTATTCTCGGCCTTGGCGAC
CAAGGTATCGGCGGTATGGGGATTCCTATCGGTAAACTGTCACTTTACACCGCTTGTGGTGGCATTAGCCCAGCGTATAC
GCTTCCTGTGGTTCTGGATGTGGGTACGAATAACCCGCAACGTCTTGCTGACCCTATGTACATGGGCTGGCGTCATCCAC
GTATCACTGGACCTGACTACGACAACTTTGTTGAAGAGTTTATGCAAGCCGTGCAACGTCGCTGGCCAGATGCATTGATC
CAGTTTGAAGACTTTGCCCAAAAGAACGCCATGCCACTGCTTGAGCGTTACAAAAATCGCGTGTGCTGTTTTAACGACGA
CATTCAGGGCACAGCGGCGGTCACTGTCGGCTCACTGCTTGCTGCCTGTAAAGCGGCGGGTAGCCAGCTTTCACAACAAC
GTATTACCTTCTTGGGCGCGGGCTCTGCAGGCTGCGGTATCGCGGAAGCGATCATTGCGCAAATGGTGTCTGAAGGCATC
AGCGATGAGCAAGCGCGCTCACAAGTTTACATGGTGGATCGCTGGGGCCTGCTCGAAGAAGGCATGCCAAATTTGCTCGA
CTTCCAACAGCGCCTAGTGCAGAAAAAAGCCAACACGCAACATTGGACAACGGAAAACAACGGCTACTCGCTGCATGATG
TGATCCGCAATGCCAAACCGACTGTACTGGTTGGTGTGTCTGGTGCGCCCGGTCTGTTTAGTGAAGAGATCATCAAAGAG
ATGCATCAGCACTGTCCACGCCCTATTGTCTTCCCGCTATCGAACCCAACAAGCCGTGTTGAAGCTCTGCCGAGCGATAT
TATCCGTTGGACTAACGGTGAGGCTTTGGTTGCAACAGGTAGCCCGTTTGATCCTGTGCTGCATGAAGGCAAAACTTACC
CAATTGTGCAATGTAACAACAGCTACATTTTCCCGGGTATCGGTTTAGGAGTTTTGGCAGCAAACGCACGCCGCGTTACC
GACGAAATGCTGATGGAGTCGAGCCGTGCACTGGCAAGTTGCTCACCGCTTGCCATCAATGGTCATGGACCATTATTGCC
GCCTCTGGAATCTATCCACTCTGTTTCGAAGAAGATTGCTTTCGCAGTAGCGAAAAAAGCGATTGAACAGGGTGTGGCTC
CAGAAGTCACCGATGAAGCGCTGGAAGCCTCAATTGAACAACATTTCTGGCAACCAGTTTACCGCCGTTATAAGCGCACT
GCGTTTTAA

Upstream 100 bases:

>100_bases
ATAATCAACAGAGGCAAGCCGTTTGTCTATTTACAATCCAACCAGCAAGTCGGTATCATTTTCGCGTTTCACTTCTACCC
TACCAATCAATAGGCCGAAC

Downstream 100 bases:

>100_bases
TTTATGCGGAGAAGCCCCTGCAATCAGGGGCTTTCTAATCTCATGCAACTATTTACTTATCTTGCTCCTGCAGGTCAGGC
TCTACTCCCCTACCTGAGCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDT
NETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVTDGERILGLGD
QGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPRITGPDYDNFVEEFMQAVQRRWPDALI
QFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQRITFLGAGSAGCGIAEAIIAQMVSEGI
SDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDVIRNAKPTVLVGVSGAPGLFSEEIIKE
MHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYPIVQCNNSYIFPGIGLGVLAANARRVT
DEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAPEVTDEALEASIEQHFWQPVYRRYKRT
AF

Sequences:

>Translated_562_residues
MNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDT
NETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVTDGERILGLGD
QGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPRITGPDYDNFVEEFMQAVQRRWPDALI
QFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQRITFLGAGSAGCGIAEAIIAQMVSEGI
SDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDVIRNAKPTVLVGVSGAPGLFSEEIIKE
MHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYPIVQCNNSYIFPGIGLGVLAANARRVT
DEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAPEVTDEALEASIEQHFWQPVYRRYKRT
AF
>Mature_562_residues
MNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDT
NETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVTDGERILGLGD
QGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPRITGPDYDNFVEEFMQAVQRRWPDALI
QFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQRITFLGAGSAGCGIAEAIIAQMVSEGI
SDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQHWTTENNGYSLHDVIRNAKPTVLVGVSGAPGLFSEEIIKE
MHQHCPRPIVFPLSNPTSRVEALPSDIIRWTNGEALVATGSPFDPVLHEGKTYPIVQCNNSYIFPGIGLGVLAANARRVT
DEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAPEVTDEALEASIEQHFWQPVYRRYKRT
AF

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family

Homologues:

Organism=Homo sapiens, GI4505143, Length=522, Percent_Identity=45.4022988505747, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI62420882, Length=562, Percent_Identity=41.6370106761566, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI62420880, Length=562, Percent_Identity=41.6370106761566, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI239049447, Length=562, Percent_Identity=41.6370106761566, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4505145, Length=554, Percent_Identity=42.057761732852, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI270265879, Length=463, Percent_Identity=44.4924406047516, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI87081919, Length=558, Percent_Identity=69.8924731182796, Blast_Score=810, Evalue=0.0,
Organism=Escherichia coli, GI1788806, Length=407, Percent_Identity=25.3071253071253, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17537199, Length=549, Percent_Identity=43.5336976320583, Blast_Score=430, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322823, Length=559, Percent_Identity=44.0071556350626, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI281362674, Length=527, Percent_Identity=43.4535104364326, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI21356279, Length=527, Percent_Identity=43.4535104364326, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI281362672, Length=527, Percent_Identity=43.4535104364326, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24646388, Length=526, Percent_Identity=41.6349809885932, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24646386, Length=526, Percent_Identity=41.6349809885932, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI78707232, Length=509, Percent_Identity=37.721021611002, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281363505, Length=511, Percent_Identity=37.3776908023483, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI78707236, Length=511, Percent_Identity=37.3776908023483, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281363503, Length=509, Percent_Identity=37.1316306483301, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78707242, Length=534, Percent_Identity=37.8277153558052, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78707238, Length=534, Percent_Identity=37.8277153558052, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78707240, Length=510, Percent_Identity=38.4313725490196, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922384, Length=553, Percent_Identity=30.0180831826401, Blast_Score=246, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAO1_VIBC3 (A5F1Z0)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F1Z0
- SMR:   A5F1Z0
- STRING:   A5F1Z0
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0809
- eggNOG:   COG0281
- HOGENOM:   HBG289821
- ProtClustDB:   PRK13529
- BioCyc:   VCHO345073:VC0395_A0809-MONOMER
- HAMAP:   MF_01619
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000106
- PRINTS:   PR00072
- SMART:   SM00919

Pfam domain/function: PF00390 malic; PF03949 Malic_M

EC number: =1.1.1.38

Molecular weight: Translated: 62154; Mature: 62154

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 101-101 ACT_SITE 172-172 BINDING 154-154 BINDING 267-267 BINDING 415-415

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQ
CCCCCCCEEEEECCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
YRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGR
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCC
GLFISYANRDRIDDLLNNAANHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACG
CEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHC
GISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPRITGPDYDNFVEEFMQAVQRRWPDALI
CCCCCEEEEEEEECCCCCHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
QFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQRITFLGA
HHHHHHHCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
GSAGCGIAEAIIAQMVSEGISDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQ
CCCCCHHHHHHHHHHHHCCCCCHHHHCEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCC
HWTTENNGYSLHDVIRNAKPTVLVGVSGAPGLFSEEIIKEMHQHCPRPIVFPLSNPTSRV
CCCCCCCCEEHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHH
EALPSDIIRWTNGEALVATGSPFDPVLHEGKTYPIVQCNNSYIFPGIGLGVLAANARRVT
HHHHHHHHEECCCCEEEECCCCCCHHHHCCCCCEEEEECCCEECCCCCHHHHHCCHHHHH
DEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAPEVTDEA
HHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LEASIEQHFWQPVYRRYKRTAF
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNDKRPLYISYAGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQ
CCCCCCCEEEEECCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
YRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGR
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCC
GLFISYANRDRIDDLLNNAANHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACG
CEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHC
GISPAYTLPVVLDVGTNNPQRLADPMYMGWRHPRITGPDYDNFVEEFMQAVQRRWPDALI
CCCCCEEEEEEEECCCCCHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
QFEDFAQKNAMPLLERYKNRVCCFNDDIQGTAAVTVGSLLAACKAAGSQLSQQRITFLGA
HHHHHHHCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
GSAGCGIAEAIIAQMVSEGISDEQARSQVYMVDRWGLLEEGMPNLLDFQQRLVQKKANTQ
CCCCCHHHHHHHHHHHHCCCCCHHHHCEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCCC
HWTTENNGYSLHDVIRNAKPTVLVGVSGAPGLFSEEIIKEMHQHCPRPIVFPLSNPTSRV
CCCCCCCCEEHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHH
EALPSDIIRWTNGEALVATGSPFDPVLHEGKTYPIVQCNNSYIFPGIGLGVLAANARRVT
HHHHHHHHEECCCCEEEECCCCCCHHHHCCCCCEEEEECCCEECCCCCHHHHHCCHHHHH
DEMLMESSRALASCSPLAINGHGPLLPPLESIHSVSKKIAFAVAKKAIEQGVAPEVTDEA
HHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LEASIEQHFWQPVYRRYKRTAF
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA